ABSTRACT
To compare the effect of 50% Aqueous and 50% Ethanolic extract of Syzygium aromaticum on blood glucose level in STZ induced diabetic rats in comparison with insulin. Randomized control trial. This study was conducted at National Institute of Health Islamabad from July 2011 to December 2011. Forty adult rats of Sprague dawaley specie were equally divided into 5 groups [I-V]. Group-1 control. Group [II-V] received a single intraperitoneal injection of STZ and rats having fasting blood glucose above 200mg/dl were selected. Group-11 served as diabetic control, group III received 50% aqueous extract at a dose of 750 mg/kg body weight for sixty days and group IVrats received 50% ethanolic extract of Syzygium aromaticum at a dose of 750 mg/kg body weight for sixty days. Group V [standard] received the dose of 0.6 units/kg body weight of humulin insulin 70/30 subcutaneously bid for sixty days. After giving the injection of STZ fasting blood samples were taken at zero 15,30 and 60 days and comparison is done between the glucose lowering effect of aqueous and ethanolic extract of Syzygium aromaticum. The 50% ethanolic extract of Syzygium aromaticum showed more reduction in blood glucose level than the 50% aqueous extract of Syzygium aromaticum. The levels of blood glucose markedly decreased in group-lV receiving 750 mg/kg body of ethanolic extract as compared to group III receiving the same dose of aqueous extract. Group V receiving insulin showed the level of this parameter almost closer to the blood glucose levels of group III rats. The reduction in blood glucose with 750 mg/kg body weight of ethanolic extract of Syzygium aromaticum is more than with aqueous extract and insulin
ABSTRACT
The purpose of the present study was to assess the effect of ethanolic extract of Syzygium aromaticum buds in streptozotocin [STZ] induced diabetes in rats. Randomized controlled trial. This study was conducted at the Department of Pharmacology, Islamic International Medical College, Rawalpindi from 1[st] Jan 2011 to 30 [st] June 2011. Single injection of STZ was given intraperitoneally to rats and rats showed fasting glucose level over 280 mg/dl were included in the study. After induction of diabetes all rats were divided into, normal control group [A], diabetes positive control group [B], and the two groups [C and D] served as experimental groups while group E served as standard as it received glibenclamide. Group C and D diabetic experimental rats received ethanolic extract of Syzygium aromaticum at 250 mg/kg and 500mg/kg of body weight orally for eight weeks on daily basis. On the other hand group E rats received glibenclamide at 0.5 mg/kg body weight orally for eight weeks. Blood samples were collected after eight weeks. The diabetic positive group rats showed variable increase in serum levels of glucose, triglycerides, low density dipoprotein [LDL] and total cholesterol levels. Serum high density lipoprotein [HDL] levels decreased in diabetic positive group. Syzygium aromaticum 250 mg/kg and 500 mg/kg dose and glibenclamide significantly decreased the levels of these parameters in rats. On comparison Syzygium aromaticum 500 mg/kg dose reduced glucose and lipid levels more, effectively than the 250mg/kg dose of Syzygium aromaticum and glibenclamide. Syzygium aromaticum constitutents, especially polyphenols and flavonoids have strong anti-oxidant activity which might be involved in glucose and lipid lowering effect. Syzygium aromaticum ethanolic extract decrease glucose and lipid levels in experimentally induced diabetic rats