ABSTRACT
Based on the patents authorized for inventions in the Chinese medicine industry from 2005 to 2016, a patent co-occurrence network is constructed using international patent classification (IPC) information; And based on the K-shell decomposition method to identify core technologies in the network, the technical structure, research hotspot, and the absorption of other technical topics in core areas of traditional Chinese medicine are analyzed. The absorption of innovation in different periods is further described to explore the development trend of the traditional Chinese medicine industry, technological growth points and the main areas of absorption of core technologies of traditional Chinese medicine, in order to provide references and suggestions for industry technological innovation of traditional Chinese medicine.
ABSTRACT
To investigate the relationship between cytoskeleton and hyposmotic membrane stretch-induced increase in muscarinic current, the role of actin microfilament in hyposmotic membrane stretch-induced increase in muscarinic current was studied with the whole-cell patch clamp technique in guinea-pig gastric myocytes. In this study, the muscarinic current was induced by carbachol (50 micromol/L) or GTPgammaS (0.5 mmol/L). The results showed that hyposmotic superfusate (202 mOsmol/L) increased carbachol-induced current (I(CCh)) by 145+/-27% and increased GTPgammaS-induced current by 183+/-30%; but in the presence of cytochalasin-B (Cyt-B, 20 micromol/L), an actin cytoskeleton disruptor, hyposmotic membrane stretch increased I(CCh) by 70+/-6%. However, hyposmotic membrane stretch induced increase in I(CCh) was potentiated to 545+/-81% by phalloidin (20 micromol/L), an actin microfilament stabilizer. The results demonstrated that hyposmotic membrane stretch increased the muscarinic currents induced by carbachol or GTPgammaS and that the actin microfilament is involved in the process in guinea-pig gastric myocytes.