ABSTRACT
Object To study the chemical constituents of Hypericum sampsonii Hance from Yunnan Province Methods Cytotoxicity screening of extracts from wild and cultivated H sampsonii was carried out by L 1210 cell and KB cell Chemical constituents for wild H sampsonii were isolated by column chromatography The chemical structures were identified by physical and chemical properties and spectral data analysis Results Six compounds have been isolated and established as 1 benzoyl 3 (3 methyl 2 butenyl) 6, 6, 13, 13 tetramethyl 11 geranyl 5 lxatetracycol[7 3 1 0 3, 7 0 4, 11 ] tridecane 2, 12 dione (sampsonione A, Ⅰ); 1 benzoyl 5 (1 hydroxy isopropyl) 6, 6, 13, 13 tetramethyl 11 geranyl tetracycol [7 3 1 1 0 3, 7 ] tetradecane 2, 12, 14 trione (sampsonione F, Ⅱ); 3 (1 hydroxy 5 methyl 4 hexenyl) 6, 10 di (3 methyl 2 butyryl) 8 benzoyl 9, 9 dimethyl 4 oxatricycol [6 3 1 0 1, 5 ] 5 dodecene 7, 12 dione (sampsonione K, Ⅲ); 1,2 dihydro 3, 6, 8 trihydroxy 1, 1 bis (3 methyl but 2 enyl) xanthen 2, 9 dione (patulone, Ⅳ); 1, 7 dihydroxy 4 methoxy xanthone (Ⅴ) and 1, 3, 6, 7 tetrahydroxy 8 (3 methyl but 2 enyl) xanthone (Ⅵ) Conclusion Compounds Ⅳ-Ⅵ are first obtained from H sampsonii In addition, the fractions of chloroform extracting and ethyl acetate extracting possess anticancer activities by cytotoxicity tests
ABSTRACT
Objective To study the chemical constituents in plants of Hypericum hookerianum and their activities by pharmacological experiment. Methods Cytotoxicity screening of the extracts from H. hookerianum were carried to isolate and purify the chemical constituents. The chemical structures were (identified) by physicochemical properties and spectral data analysis. Results Seven compounds were isola-(ted) and (established) as quercetin (Ⅰ), luteolin (Ⅱ), apigenin (Ⅲ), hyperin (Ⅳ), astragalin (Ⅴ), hyper-(olactone) A (Ⅵ), and hyperolactone C (Ⅶ). Conclusion All the compounds are isolated from H. hookerianum for the first time. Compounds Ⅲ and Ⅴ are isolated from the plants of Hypericum Linn. for the first time. In addition, the fractions by chlorform and ethylacete extracting, quercetin, and luteolin possess the cytotoxicity.