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To develop and evaluate an ELISA kit for Anti-PLA2R IgG. Recombinant M-type phospholipase A2 receptor (PLA2R) protein expressed in HEK293 was taken as coating antigen, HRP-labeled rabbit anti-human IgG was taken as a tracer, to test the anti-PLA2R IgG on the basis of the principle of ELISA. The detection linear range, accuracy, linear correlation, repeatability, and stability were evaluated. In addition, we made a comparison with enrolled anti-PLA2R IgG kit. The detection linear range of the kit is no more than 2 RU/mL. The relative deviation of the kit accuracy is in -15%-15%. There is a linear correlation coefficient of higher than 0.990 0 within 2-500 RU/mL range. The CV of the repeatable test is lower than 15% when the kit was put in 37 ℃ one week, 2-8 ℃ one year, the performance remains. The consistency of testing with comparison in enrolled anti-PLA2R IgG kit is 98.9% (positive: 97.2%, negative: 100%). The Anti-PLA2R IgG ELISA Kit which we developed is nearly identical to the reference standard in specific and sensitive of clinics. It's successfully used to determine anti-PLA2R titer and help clinical diagnosis.
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Objective To assess the association of three polymorphisms in Megsin (rs1055901,rs1055902 and rs2689399) and susceptibility of IgA nephropathy in Asian population.Methods We conducted a comprehensive search of electronic CNKI,VIP,WangFang Data,CBM,Pubmed,Web of Science and Google Scholar database on the association between Megsin rs1055901,rs1055902 and rs2689399 polymorphism and susceptibility of IgA nephrology in Asian population (last search update on 2 May 2016).Stata 12.0 software was used to calculate the odds ratio (OR) and 95 ％ CI (confidence interval),as well as sensitivity and publication bias analyses.Results Six publications encompassing mine case-control studies were finally included,including 2 179 cases and 1 769 controls.Finally,no significant association between Megsin rs1055901 and rs1055902 polymorphism and IgA nephrology in Asian population was identified,while a significantly decreased risk of IgA nephrology for rs2689399 polymorphism,was identified in Asian population (G and C:OR=0.754,95％CI 0.592-0.961,P=0.022;GG and CC:OR=0.506,95％CI 0.287-0.892,P=0.019;GG and GC+CC:OR=0.551,95％CI 0.316-0.961,P=0.036).Conclusion Rs2689399 G allele and GG genotype of Megsin may be the protective factors for IgA nephropathy in Asian population.
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Objective To preliminary screen serum microRNA which was related to class Ⅳ and class Ⅳ+Ⅴ lupus nephritis (LN)by using microRNA array technology,and explore the role of serum microRNA in renal pathological classification of lupus nephritis.Methods Eight serum samples of lupus nephritis patients from biological specimen library in our hospital were enrolled. Experimental group was composed of 4 class Ⅳ cases and 4 class Ⅳ+Ⅴ cases,control group was composed of 4 healthy persons. MicroRNA array was used to screen the differentially expressing microRNA.Results There were part of same miRNA in class Ⅳand classⅣ+Ⅴ LN,among which expression of 22 miRNA were significantly different,29 miRNA of classⅣ was unique,3 miRNA of class Ⅳ+Ⅴ was unique.Conclusion Serum miRNA has a potential to serve as a biomarker to identify class Ⅳ and classⅣ+Ⅴlupus nephritis.
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Objective To investigate the clinical effect of intravenous iron dextran peritoneal dialysis patients with renal anemia.Methods Fifty cases of peritoneal dialysis patients with renal anemia in the Shenzhen Hospital of Peking University from Jan.2010 to Jun.2014 were randomly divided into control group and the observation group,and 25 cases in each group.Patients in observation group were given intravenous iron dextran at dose of 100 mg/time,and 3 times/week.Patients in control group were given ferrous succinate at dose of 200 mg/times orally,and 3 times/day.Both treatment period was eight weeks.Changes in clinical symptoms and treatment of the two groups of patients were recorded.Results Ater eight weeks treatment,the levels of hemoglobin(Hb),red blood cell count (RBC),hematocrit (Hct),reticulocyte count (Ret),total iron binding capacity(TS) and serum iron(SF) in two groups were significantly better than before treatment (P＜0.05 or P ＜0.01).The levels of Hb,RBC,Hct,Ret,TS and SF in observation group after treatment were (91.6± 13.8) g/L,(3.4± 0.4) × 109/L,0.31 ± 0.04,(1.7 ± 0.6) ％,(38.9 ± 6.7) ％ and (525.9 ± 81.4) μg/L,significantly better than those of control group((79.6 ± 13.9) g/L,(3.0±0.7) × 109/L,0.27±0.06,(1.4 ±0.6) ％,(28.1 ±8.4)％ and (377.2 ± 107.2) μg/L),and the differences were statistically significant (P ＜0.05).Total effective rate of observation group were 80％(20/25) and 76％(19/25) in the control group.The difference was not statistically significant(x2 =4.364,P＞0.05).Adverse reactions in observed group was 4％ (1/25) and 8％ (2/25) in control group (x2 =4.895,P ＞ 0.05).Conclusion The therapy of intravenous iron dextran on peritoneal dialysis patients with renal anemia can effectively improve the clinical symptoms,and supply iron for the patient.
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Objective The clinical data of postoperative perirenal hematoma after renal biopsy in recent 10 years were retrospectively analyzed,and its relationship with pathological type was explored.Methods From April 2003 to April 2013,2062 patients of renal biopsy were enrolled and divided into 3 groups:youth group (18-39 years,1634 cases),middle age group (40-59 years,323 cases) and aged group (≥60 years,105 cases).Relationship between renal hematoma and pathology was analyzed.Results There were 1370,255,69 cases of primary glomerular disease respectively in 3 groups,and 264,68,36 cases of secondary glomerular diseases.Three hundred and seventy-nine in all patients were complicated with perirenal hematoma,and the incidence rates were 15.8％ (325/2062),1.8％ (37/2062),0.8％ (17/2062) respectively.Incidence rate of hematoma in primary glomerular disease was higher than that in secondary diseases [19.0％ (322/1694) vs.15.5％ (57/368)].Three most common primary glomerular disease in which perirenal hematoma occured was IgA nephropathy 7.4％ (126/1694),focal/segmental lesions 4.2％(71/1694) and membranous nephropathy 2.4％ (41/1694); while the incidence rate of lupus nephritis hematoma was as high as 9.0％ (33/368).Conclusion Single-center data shows that the most common pathology types of perirenal hematoma are lupus nephritis,IgA nephropathy,focal/segmental lesions and membranous nephropathy.
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Objective: To investigate peroxisome proliferator activated receptor ? (PPAR ?) expressions in patients with actively proliferating glomerulonephritis such as type Ⅳ lupus nephritis (LN) and cellular crescentic glomerulonephritis (RPGN). Methods: All patients were divided into 4 groups as follows: RPGN (17 cases); LN type Ⅳ (15 cases); mild mesangial proliferative IgA nephropathy (IgAN, 7 cases) and minimal change disease (MCD, 10 cases). Clinical parameters, immunohisto chemistry stain and in situ hybridization of renal biopsy specimens were performed. Results: Clinically, proteinuria and hematouria were increased and Ccr were decreased in LN and RPGN patients, and increased ESR and decreased complement C3 were found in group LN. Active index of renal specimens were significantly higher in LN and RPGN groups than in IgAN and MCD groups. Renal specimens of MCD patients showed no positive PPAR ? staining in all sections; little immunoreactivity was detected in sections of glomerular, tubular and interstitial cells from IgAN patients. Glomerular positive staining of PPAR ? in renal sections in LN and RPGN patients[(3.3?1.8) and (2.8?1.2) cells per section of glomerulus, respectively] were significantly increased compared with that in IgAN patients [(0.7?0.5) cells per section of glomerulus]. Similarly, tubular positive staining of PPAR ? in LN and RPGN patients (27.38? 12.46, 23.36?10.55) were also elevated compared with that in IgAN and MCD patients(6.51?3.49, 1.72?0.31). The relevance assay results showed positive relationship between active index and glomerular or tubular PPAR ? immunohisto chemistry staining cell numbers (0.478, P
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AIM To explore effects of protein kinase C (PKC) inhibitor breviscapine on protein expression of c fos?c jun and collagen Ⅳ(C Ⅳ) production in rat glomerular mesangial cells (GMC) cultured under high glucose conditions. METHODS Rat GMCs were cultured under normal glucose, high glucose or high glucose and breviscapine. After 24 h, 48 h or 1 wk, protein expression of c fos?c jun, C Ⅳ and PKC activity were measured. RESULTS Increased protein expression of c fos?c jun, C Ⅳ and PKC activity were observed under high glucose compared to normal glucose. All the above mentioned effects of high glucose were inhibited completely or partly by breviscapine. CONCLUSION High glucose may increase protein expression of c fos?c jun and C Ⅳ production mediated by PKC activation in cultured GMCs. PKC inhibitor breviscapine may ameliorate these abnormalities efficiently.