ABSTRACT
OBJECTIVES@#To observe the clinical efficacy of timing umbilical therapy for neurogenic bladder after spinal cord injury based on the midnight-noon and ebb-flow doctrine.@*METHODS@#Sixty patients with neurogenic bladder after spinal cord injury were randomly divided into a trial group and a control group, with 30 patients in each group. In the trial group, based on the midnight-noon and ebb-flow doctrine, umbilical therapy was given at the time zone, 15:00 to 17:00. In the control group, umbilical therapy was delivered at any time zones except the period 15:00 to 17:00. The herbal plaster was remained on the umbilicus for 4 h each time, once daily. One course of treatment was composed of 2 weeks and the treatment lasted 4 weeks. Before and after treatment, the urodynamic indexes (maximum urinary flow rate [Qmax], maximum detrusor pressure [Pdet-max], residual urine volume [RUV]), voiding diary (average daily number of voiding, average daily number of leakage, average daily voided volume), neurogenic bladder symptom score (NBSS), the score of urinary symptom distress scale (USDS) and the score of World Health Organization quality of life assessment-BREF (WHOQOL-BREF) were compared between the two groups; and the clinical efficacy of the two groups was assessed.@*RESULTS@#After treatment, Qmax, Pdet-max, the average daily voided volume and the scores of WHOQOL-BREF were increased (P<0.05); and RUV, the average daily number of voiding, the average daily number of leakage, NBSS and the scores of USDS were all reduced (P<0.05) in comparison with those before treatment in the two groups. When compared with those in the control group, Qmax, Pdet-max, the average daily voided volume and the score of WHOQOL-BREF were all higher (P<0.05); and RUV, the average daily number of voiding, the average daily number of leakage, NBSS and the score of USDS were lower (P<0.05) in the trial group. The total effective rate was 96.7% (29/30) in the trial group, higher than that (76.7%, 23/30) in the control group (P<0.05).@*CONCLUSIONS@#Timing umbilical therapy, based on the midnight-noon and ebb-flow doctrine, effectively relieves the symptoms of dysuria and improves the quality of life in patients with neurogenic bladder after spinal cord injury.
Subject(s)
Humans , Urinary Bladder, Neurogenic/therapy , Quality of Life , Umbilicus , Urinary Bladder , Spinal Cord Injuries/complicationsABSTRACT
Objective To investigate PLCE1 protein expression in esophageal squamous cell carcinoma (ESCC)tissues,and understand the effect of PLCE1 protein expression on the prognosis of patients with ESCC.Methods The PLCE1 protein were detected in 85 patients with surgically resected ESCC paraffin-embedded tissue using immunohistochemical staining,the patients' information were selected from March 1997 to December 2011 in the database of the Henan Key Laboratory for Esophageal Cancer Research,and the survival analysis were operated with Kaplan Meier single factor and Cox multivariate regression.Results In the 85 cases,the lost to follow-up were 5 cases,the follow-up rate was 94.1％ (80/85).In the 80 cases with ESCC,the positive expression rate of PLCE1 was 77.5％ (62/80).PLCE1 positive expression was not significantly associated with differentiation,infiltration depth,lymph node metastasis and family history with ESCC (P ＞ 0.05).The univariate survival analysis by Kaplan-Meier showed that protein expression of PLCE1 had no significant association with overall survival (P ＞ 0.05).Cox regression survival analysis showed that the lymph node metastasis was associated with the survival time of ESCC patients [the relative risk were 1.763,95％ CI(1.008,3.084),P =0.047].Conclusion The expression of PLCE1 protein was involved in mechanism of ESCC,and might not predicted the prognosis of the patients with ESCC.
ABSTRACT
<p><b>OBJECTIVE</b>To identify the relationship between amino acid mutations in Neisseria gonorrhoeae isolates and their antibiotic resistance.</p><p><b>METHODS</b>PI gene fragments of Neisseria gonorrhoeae from 17 clinical isolates were obtained with PCR amplification. They were cloned into the PCR cloning vector pBS-T to form pBS-T-PI and sequenced. The sequences of PI genes were analyzed. At the same time, minimum inhibitory concentration (MIC) of penicillin and tetracycline to these 17 isolates were measured and contrasted with the corresponding PI sequence.</p><p><b>RESULTS</b>The recombinants of PI gene from 17 clinical isolates of Neisseria gonorrhoeae were successfully constructed and sequenced. They were divided into PIA and PIB subtypes according to the results from blastn software by comparing the sequences with the GenBank. Mutations were found at the sites of 120 and 121. There were only some of the sequences having an aspartic acid (D) mutation on 120 and 121 sites, which was not the same as reported. On the other hand,there were two PI sequences,5-9 and 6-1, whose mutations on No. 120 were lysine, similar to those documented.</p><p><b>CONCLUSION</b>Some relationship between PI amino acids mutations at sites 120 and 121 in Neisseria gonorrhoeae isolates from Chengdu, China and their resistance to penicillin and tetracycline were found. However,further studies need to be done in the future to confirm this hypothesis.</p>