ABSTRACT
AIM:To investigate the expression of microRNA (miRNA)-93 in acute lymphocytic leukemia (ALL) and its effect on the proliferation of acute T-cell leukemia Jurkat cells.METHODS:The expression of miRNA-93 in the bone marrow samples of patients with ALL was measured by real-time PCR.After down-regulation of miRNA-93 by transfection with miRNA-93 inhibitor in the Jurkat cells,the cell viability,cell proliferation and cell cycle distribution were detected by CCK-8 assay,EdU assay and flow cytometry,respectively.Furthermore,the protein levels of cell cycle-related molecules such as cyclin D1,cyclin-dependent kinase 4 (CDK4),phosphorylation retinoblastoma (Rb) and P27 were measured by Western blot.RESULTS:miRNA-93 was highly expressed in the patients with ALL,and the expression level was highest in the high risk patients.Down-regulation of miRNA-93 inhibited Jurkat cell viability,arrested cell cycle in G1/S transition.In addition,the protein levels of cyclin D1,CDK4 and p-Rb were significantly decreased,the protein expression of P27 was increased in Jurkat cells trasfected with miRNA-93 inhibitor.CONCLUSION:miRNA-93 expression is increased in ALL patients.Down-regulation of miRNA-93 restrains cell proliferation in the acute T cell leukemia cell line Jurkat via regulating cell cycle-related molecules.
ABSTRACT
AIM:To investigate the expression of microRNA (miRNA)-93 in acute lymphocytic leukemia (ALL) and its effect on the proliferation of acute T-cell leukemia Jurkat cells.METHODS:The expression of miRNA-93 in the bone marrow samples of patients with ALL was measured by real-time PCR.After down-regulation of miRNA-93 by transfection with miRNA-93 inhibitor in the Jurkat cells,the cell viability,cell proliferation and cell cycle distribution were detected by CCK-8 assay,EdU assay and flow cytometry,respectively.Furthermore,the protein levels of cell cycle-related molecules such as cyclin D1,cyclin-dependent kinase 4 (CDK4),phosphorylation retinoblastoma (Rb) and P27 were measured by Western blot.RESULTS:miRNA-93 was highly expressed in the patients with ALL,and the expression level was highest in the high risk patients.Down-regulation of miRNA-93 inhibited Jurkat cell viability,arrested cell cycle in G1/S transition.In addition,the protein levels of cyclin D1,CDK4 and p-Rb were significantly decreased,the protein expression of P27 was increased in Jurkat cells trasfected with miRNA-93 inhibitor.CONCLUSION:miRNA-93 expression is increased in ALL patients.Down-regulation of miRNA-93 restrains cell proliferation in the acute T cell leukemia cell line Jurkat via regulating cell cycle-related molecules.