ABSTRACT
Aim To investigate the antitumor effects of cimigenol ( KY17 ) , a novel cycloartane triterpenoid from Cimicifuga , in human colon cancer cells (HCT116).Methods MTT assay was used to deter-mine the effect of KY17 on the proliferation of mouse embryonic fibroblasts ( MEF) and human colon cancer HCT116 cell line.Flow cytometry was employed to de-tect the effect of KY17 on HCT116 cell cycle.Fluores-cence microscopy and flow cytometry were used to ana-lyze the apoptosis .Western blot was used to detect the expression of apoptotic protein (PARP).q-PCR ana-lyzed the expression of miRNA-34a.Results The IC50 of KY17 in MEF and HCT116 cells was 27.28 μmol· L-1 and 9.31μmol· L-1 , respectively.KY17 induced HCT116 cell cycle arrest in G2/M phase and the apoptotic protein PARP cleavage . In addition, KY17 up-regulated the expression of p 53 protein and miRNA-34a.Conclusions KY17 inhibits the prolifera-tion and the cell cycle is arrested in G 2/M, inducing the apoptosis of HCT116 cells. The mechanism is probably related to miRNA-34a up-regulation and p53 activation .
ABSTRACT
Chinese medicine (CM) basic theory differs substantially from modern medicine in methodology. CM views the healthy human body as an entity in yinyang equilibrium. CM practitioners typically diagnose patients with "Zheng (CM syndrome)"-yin-yang status of specific organs, and the ultimate goal of treatment in CM is to restore the yin-yang balance. This concept might seem strange to Western perceptions; however, CM philosophy and methodology have been gradually accepted and used in the West. There is growing evidence from prestigious worldwide journals like Nature, Science and Cell indicating that CM theory is becoming an important component in dealing with health issues in the West.