Fertility preservation in nonepithelial ovarian malignancy / 中国实用妇科与产科杂志

Non-epithelial ovarian malignant tumors account for about 10% of ovarian tumors,and mostly develops in young women.Although surgery and radiotherapy and chemotherapy can make patients get the chances of survival,but often lead to different degrees of damage to reproductive function,causing physical and psychological suffering.While improving the survival rate of patients with malignant ovarian tumors,it is an important research issue to protect the reproductive function of patients as much as possible in the treatment of malignant ovarian tumors.The fertilitysparing treatment has a good effect on non-epithelial ovarian malignant tumors,especially malignant ovarian germ tumors.In this article,we discuss the surgical treatment of fertility protection,the implementation of postoperative adjuvant treatment and the application of assisted reproductive technology in non-epithelial ovarian malignant tumors.

Beclin-1 expression is upregulated by spermine in rat myocardial ische-mia-reperfusion injury / 中国病理生理杂志

AIM:To observe the effects of spermine(SP)on myocardial ischemia-reperfusion(IR)injury in rats.METHODS:SD rats(weighing 220~250 g)were equally randomized to 3 groups: sham control group, in which the rats were only treated with thoracotomy;IR group,in which the rats were treated with ischemia for 30 min and reperfu-sion for 60 min;and IR+SP group,in which 0.5 mmol/L SP(2 mL/kg)was intravenously injected just 15 min before reperfusion.The morphological changes of myocardial tissues were assessed by HE staining.The levels of cardiac troponin I(cTnI)and creatine kinase isoenzyme MB(CK-MB)in plasma were determined.Myocardial infarct size and no-reflow range of the myocardium were measured by Evans blue and thioflavin S staining.Inflammatory responses in the myocardial tissues were detected by myeloperoxidase(MPO)assay.The autophagy function was detected by measuring the protein ex-pression of beclin-1 by Western blot.RESULTS: The myocardial injury and inflammatory infiltration in IR +SP group were reduced under light microscope.Treatment with SP decreased the plasma levels of cTnI and CK-MB,and reduced the IR-induced infarct size and no-reflow range size of the left ventricle(P<0.05).Tissue MPO assay showed that myocardial inflammatory responses were attenuated in IR +SP group compared with IR group.Beclin-1 was upregulated in IR +SP group compared with IR group(P<0.05).CONCLUSION: Exogenous SP attenuates myocardial ischemia-reperfusion injury by upregulating the expression of beclin-1.

Effect of glucagon-like peptide-1 on hypoxia-reoxygenation induced injury in neonatal rat cardiomyocytes / 中华心血管病杂志

<p><b>OBJECTIVE</b>To observe the effect of glucagon-like peptide-1 (GLP-1) on hypoxia-reoxygenation (H/R) induced injury in neonatal rat cardiomyocytes.</p><p><b>METHODS</b>Cultured neonatal rat cardiomyocytes were randomly divided into seven groups: normal control group, H/R group, GLP-1 + H/R group, GLP-1 + H/R + UO126 group, GLP-1 + H/R + LY294002 group, H/R + UO126 group, H/R + LY294002 group. LDH activity, apoptosis rate of cardiomyocytes, Caspase-3 activity were detected.</p><p><b>RESULTS</b>Compared with normal control group, the activity of LDH, cardiomyocyte apoptosis rate, Caspase-3 activity were all significantly increased in H/R group (all P < 0.01). However, compared with H/R group, these changes were significantly attenuated in GLP-1 + H/R group [the activity of LDH (128.47 +/- 7.96) U/L vs. (223.96 +/- 22.10) U/L, P < 0.01, and cardiomyocyte apoptosis rate (2.84 +/- 2.56)% vs. (12.58 +/- 6.69)%, P < 0.01, and Caspase-3 activity (36,809 +/- 4750) RLU vs. (57,602 +/- 9161) RLU, P < 0.01], while LY294002 (PI3K inhibitor) and UO126 (MAPK inhibitor) could block the effects of GLP-1 in cardiomyocytes underwent H/R injury.</p><p><b>CONCLUSIONS</b>GLP-1 could protect H/R injury mainly by inhibiting cardiomyocytes apoptosis via activating PI3K/Akt and MAPK signaling pathway.</p>

Protective effect of estrogen against chemotherapy-induced ovarian damage in rats / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the protective effect of estrogen against cyclophosphamide (CTX)-induced ovarian failure in female rats.</p><p><b>METHODS</b>Sixty female Wistar rats (2-3 months old) were randomized into 4 groups to receive treatments with normal saline, CTX, estradiol (E2) or CTX+E2. Serum estradiol and follicle-stimulating hormone (FSH) concentrations were measured regularly in the 4 groups, and the weight of the ovary and uterus, follicle number and mean diameter of the follicles were compared.</p><p><b>RESULTS</b>Compared with CTX+EE2 group, the CTX group showed significantly increased FSH levels and decreased EE2 levels (P<0.05). The weight of the ovary and uterus and follicle number were significantly lower in CTX group than in CTX+EE2 group (P<0.05). No obvious differences in the indexes were found between the control and CTX+E2 groups.</p><p><b>CONCLUSION</b>Estrogen administration provides protection against CTX-induced ovarian damage in rats.</p>

Myocardial polyamine metabolism and the ischemia-reperfusion injury in the rat heart / 中华心血管病杂志

<p><b>OBJECTIVE</b>To observe the polyamines metabolism changes in rat cardiomyocytes underwent ischemia-reperfusion (I/R) injury.</p><p><b>METHODS</b>A branch of the descending left coronary artery was occluded to induce rat myocardial I/R injury (30 min ischemia followed by 2 h, 6 h, 12 h, and 24 h reperfusion). RT-PCR and Western blot were performed to detect the expression of spermidine/spermine N1-acetyltransferase (SSAT) and ornithine decarboxylase (ODC), the concentrations of polyamines were measured with high performance liquid chromatography in hearts with or without I/R.</p><p><b>RESULTS</b>The myocardial transcription and expression of SSAT and ODC were significantly upregulated. Compared with the sham group, ODC mRNA and SSAT mRNA respectively increased 3.1 fold and 3.8 fold and their proteins respectively increased 3.1 fold and 2.9 fold at 24 h of reperfusion (P < 0.01); the concentrations of spermidine, spermine and the total polyamine pool respectively decreased by 33.6%, 35.3% and 32.9% while putrescine concentration increased by 58.9% at 24 h of reperfusion (P < 0.01).</p><p><b>CONCLUSION</b>Our results suggest that ischemia-reperfusion in the heart may affect polyamine metabolism and the disturbance of polyamine metabolism might thus play a critical role in myocardial I/R injury in this model.</p>

Effect of artemisinin on ischemia/reperfusion injury of isolated rat myocardium / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effect of artemisinin on the ischemia/reperfusion injury of the iisolated rat myocardium and to preliminarily study the possible mechanism.</p><p><b>METHOD</b>Fifty Wistar rats were randomly divided into 5 groups: a control group, an ischemia/reperfusion (I/R) group, and 3 artemisinin (AS) groups (10, 100, 1000 micromol x L(-1)), 10 rats in each group. Ischemia/reperfusion injury of the isolated rat myocardium was induced by a Langendorff system. The electrocardiogram, the cardiac functional parameters, coronary flow, and the activities of LDH (lactate dehydrogenase), CPK (creatine phosphokinase), SOD (superoxide dis-mutase) and the level of malondiadehyde (MDA) in myocardial tissue, and the myocardial ultrastructures were investigated.</p><p><b>RESULT</b>AS (10,100 micromol x L(-1)) could significantly improve the index of the myocardial function (+/- dp/dt(max), LVSP) after the ischemia/reperfusion, increase the coronary flow, decrease the leakage of LDH and CPK, and increase the SOD activity and decrease the MDA level in cardiac tissues, and alleviate the myocardial ultrastructure injury. But, AS (1000 micromo x L(-1)) did not have the above effects.</p><p><b>CONCLUSION</b>AS (10, 100 micromol x L(-1)) alleviate the myocardial ischemia/reperfusion injury in rats. The mechanism may be related to its functions of antioxidation and scavenging free radicals.</p>

Association between calcium-sensing receptor protein expression and rat cardiomyocyte apoptosis / 中华心血管病杂志

<p><b>OBJECTIVE</b>To investigate the relationship between calcium-sensing receptor protein (CaSR) expression and rat cardiomyocyte apoptosis and related signal transduction pathways.</p><p><b>METHODS</b>The CaSR, BCl2, Caspase3 protein and ERK1/2 phosphorylation or non-phosphorylation were detected by Western blot. Cardiomyocyte apoptosis was detected by flow cytometry and immunofluorescence.</p><p><b>RESULTS</b>CaSR protein was detected in rat cardiac tissue and CaSR activator gadolinium (GdCl3) induced cardiomyocyte apoptosis and increased ERK1/2 phosphorylation and expression of BCl2 and activated Caspase3. The selective mitogen-activated protein kinase (MAPK) inhibitor PD98059 abolished gadolinium -induced ERK1/2 activation and BCl2 expression, further increased the activation of Caspase3 and cardiomyocyte apoptosis.</p><p><b>CONCLUSION</b>Our results demonstrate the CaSR existence in cardiomyocytes and CaSR activation by gadolinium can induce myocyte apoptosis by activating Caspase3 and tyrosine protein kinase pathway.</p>

Clinical Characteristics and Outcome of Cytomegalovirus Infection in Infants / 实用儿科临床杂志

Objective To study the clinical characteristics and outcome of cytomegalovirus(CMV) infection in infants.Methods All data including time of infection,clinical characteristics,laboratory tests and outcome of CMV infections in hospitalized infants were collec-(ted) and analyzed from January,1994 to July,2004.Results In 87 infected infants,congenitally infected newborns,perinatal infection in infants and postnatal infection in infants accounted for 27.6%,62.0%,16.6%,respectively.CMV hepatitis was the most frequent type of disease with the incidence of 41.3%,in which the incidence of splenomegaly was 10.3%.Most of CMV hepatitis infants had a good prognosis with the improved rate 80.5%.Central nervous system abnormality(including abnormal intension of muscle,convulsion,ocular and hearing abnormalities) occurred only in congenital and perinatal infection with the incidence of 20.4%.Generalized infection,the incidence of congenital infection and perinatal infection was 16.7%,1.8%,respectively.It did not occur in postnatal infection.The mortality rate of congenital infection and perinatal infection were 12.5% and 1.85%,respectively.Conclusions CMV infection is the main cause of infant hepatitis and it can also cause neurologic sequelae.The outcome of generalized infection in congenital infection is bad and the mortality rate is high.

Bioremediation of quinoline-contaminated soil using bioaugmentation in slurry-phase reactor / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the possibility of using bioaugmentation as a strategy for remediating quinoline-contaminated soil.</p><p><b>METHODS</b>Microorganisms were introduced to the soil to assess the feasibility of enhancing the removal of quinoline from quinoline-contaminated soil. Slurry-phase reactor was used to investigate the bioremediation of quinoline-contaminated soil. HPLC (Hewlett-Packard model 5050 with an UV detector) was used for analysis of quinoline concentration.</p><p><b>RESULTS</b>The biodegradation rate of quinoline was increased through the introduction of Burkholderia pickettii. Quinoline, at a concentration of 1 mg/g soil, could be removed completely within 6 and 8 hours with and without combined effect of indigenous microbes, respectively. Although the indigenous microbes alone had no quinoline-degrading ability, they cooperated with the introduced quinoline-degrader to remove quinoline more quickly than the introduced microbes alone. Bioaugmentaion process was accelerated by the increase of inoculum size and bio-stimulation. The ratio of water to soil in slurry had no significant impact on bioremediation results.</p><p><b>CONCLUSION</b>Bioaugmetation is an effective way for bioremediation of quinoline-contaminated soil.</p>

Vitamin K deficiency in low-birth weight premature infants and its relationship with intraventricular hemorrhage / 实用儿科临床杂志

Objective To study the incidence of vitamin K deficiency in low-birth weight premature infants and its relationship with intraventricular hemorrhage.Methods We use emzymoimmunoelectrophoresis to detect prophrombin protein precursors(PIVKA-Ⅱ) in vein blood in premature infants