ABSTRACT
Objective To detect miR-125a/b-5p expression in serum and urine of patients with preeclampsia,explore the role of miR-125a/b-5p in peripheral circulation on the pathogenesis of preeclampsia.Methods Samples of serum and urine of pa-tients with preeclampsia(n=20),and the normal pregnancy group(n=20)were collected and the expression of miR-125a/b-5p was detected by stem-loop-based real-time fluorescence quantitative reverse transcriptase polymerase chain reaction.Re-sults The expression of miR-125b-5p in the serum of patients with preeclampsia were significantly decreased compared with the normal pregnancy(Z=-2.272,P=0.023).There were no statistically differences of the expression of miR-125a-5p in serum between the two groups(Z=-0.622,P=0.547).The differences of urinary miR-125a/b-5p between patients with preeclampsia and normal pregnancy were not statistically significant(Z=-0.663,-0.189,P>0.05).Urinary miR-125b-5p was negatively correlated with diastolic blood pressure in preeclampsia group(r=-0.513,P=0.021).Serum miR-125b-5p was positively correlated with urea nitrogen in normal pregnancy group(r=0.472,P=0.036).Urinary miR-125a/b-5p were positively correlated with systolic blood pressure in normal pregnancy group(r=0.526,0.502,P=0.017,0.024). Conclusion The decrease of serum miR-125b-5p was associated with the pathogenesis of preeclampsia.
ABSTRACT
Objective To investigate the relationship between lipoprotein-related phospholipase A2(Lp-PLA2),homocysteine (Hcy),beta-N-1-deoxy fructosyl component of hemoglobin(HbAlc),low density lipoprotein cholesterol(LDL-C)and cere-bral microbleeds in patients.Methods Patients with CMBs were consecutively recruited from the Department of Neurology, in Shaanxi Provincial People's Hospital by using the diagnostic criteria according to the diagnosis and treatment of cerebral cerebrovascular disease in China in 2014.In addition,volunteers were included as normal controls.The demographic charac-teristics and the history of patients were collected.The level of Lp-PLA2,Hcy,HbA1c and LDL-C in each group was ana-lyzed.Results 62 patients with CMBs were included in this study,including 48 males and 14 females.30 patients were in-cluded into the normal control group,including 20 males and 10 females.The levels of Lp-PLA2,Hcy and HbA1c in the CMBs group were significantly higher than those in the control group(t=2.67,2.97,3.24,P<0.05).The level of HbA1c levels were correlated with the number of CMBs(r=0.287,P=0.024).The level of HbA1c was correlated with the num-ber of CMBs in deep white matter(r=0.304,P=0.016),while it was not correlated with the number of CMBs in the cortex (P>0.05).Conclusion The levels of Lp-PLA2,Hcy and HbA1c in CMBs were higher than those in normal control group. HbA1c levels were associated with the number of CMBs,especially in deep white matter.