ABSTRACT
To investigate the effects of lipopolysaccharide (LPS) on autophagy in rat hepatic stellate cells (HSC-T6) and involvement of NF-κB pathway in it.Methods 1)HSC-T6 cells were treated with LPS at the concentraction of 0, 0.01, 0.1, 1, and 10 mg/L for 0, 3, 6, 12, 18, and 24 h respectively, Microtubule-associated protein light chain Ⅱ (LC3Ⅱ) and Beclin1 levels were detected by Western blot; 2)HSC-T6 cells were randomized into groups of control group, LPS group, PDTC group, LPS+PDTC group, PMA group and LPS+PMA group, Western blot assay was used to measure the levels of LC 3Ⅱand Beclin1, immunofluoresence was used to measure NF-κB P65 intracellular distribution.The levels of hydroxyproline (Hyp) were determined by the method of colorimetry and the levels of laminin (LN) and hyaluronic acid (HA) were determined by ELISA in the culture supernatants after corresponding processing .Results The levels of LC3Ⅱand Beclin1 were significantly increased after HSC-T6 cells were treated with LPS at the concentraction of 0.1 mg/L for 6 h and the levels of Hyp, LN, and HA in the culture supernatants increased remarkably as well (P<0.05).PDTC pretreatment increased the levels of LC3Ⅱand Beclin1 in the LPS-treated HSC-T6 cells while decreased the levels of Hyp , LN, and HA significantly (P<0.05).PMA pretreatmen decreased the levels of LC 3Ⅱand Beclin1 in the LPS-treated HSC-T6 cells while increased the levels of Hyp, LN, and HA (P<0.05).Conclusions LPS can promote autophagy and activation of NF -κB pathway in HSC-T6 cells.Activation of NF-κB pathway may inhibit the LPS-induced autophagy of HSC-T6 cells.
ABSTRACT
Objective To investigate the relationship of carotid intimal-medial wall thickness(IMT) and the expression of IL-6 and Fetuin-A in two type diabetes.Methods 80 patients with two type diabetes were chosen,and 60 cases healthypeople of examination for the control group.The levels of IL-6 and Fetuin-A were measured,and measured the intimal-medial thickness (IMT) of internal carotid artery by carotid duplex ultrasonography scanning simultaneously.Results The level of IL-6 was significantly higher in two type diabetes than those in control group,and the level of Fetuin-A was declind (t=8.34 ～15.65,all P＜0.05).IL-6 level in normal IMT (A group,20 cases) was 2.24±0.21 pg/L,Fetuin level was 5.41±0.32 ng/ ml.IL-6 level in abnormal IMT (B group,18 cases) was 3.44±0.18 mm,and Fetuin level was 3.86±0.42 ng/ml.Relatively,IL-6 level in IMT with plaque (C group,22 cases) was 4.95-±-0.31 ng/ml,Fetuin-A level was 2.41±0.32 ng/ml.IL-6 level in lumen narrow (D group,20 cases) was 5.35±0.31 ng/ml,and Fetuin-A level was 2.02 ± 0.08 ng/ml.There were obvious differences for four groups to detect IL-6 was Fetuin-A levels (F=8.69 ～ 11.02,all P＜ 0.05).The level of IL-6 were rised little by little from A group to D group.There were obvious differences for comparison among groups (t=5.32～9.01,all P＜0.05).The level of Fetuin-A were declined little by little from A group to D group,there were obvious differnce for comparison among groups (t=6.14～11.53,all P＜0.05).Conclusion There was a close correlation between IL-6,Fetuin-A level and carotid intimal-medial wall thickness(IMT) in two type diabetes.To detection IL-6 and Fetuin-A levels is a target to distinguish two type diabetes whether or not with atherosclerosis.
ABSTRACT
Objective To evaluate the treatment of hypervascular hepatic metastasis with TACE. Methods One hundred and twenty nine cases of hepatic metastasis treated by TACE were selected retrospectively and then analyzed the survival rate,clinical effectiveness and lipidol deposition quantity in tumor.Results Malformation of tumor vessels and rich blood supply were found in all cases of this study.The survival rates of 6 months,1 year and 3 years were 100%,73.6% and 26.4% respectively.The clinical effective rate was 68.2%(88/129)and no-progress rate was 23.3%(30/129).The satisfactory lipidol deposition quantity was obtained in 80.9%(97/129).Conclusions TACE is a favorable method for hepatic metastasis,and discerning the hypervascular subgroup could improve the treating effectiveness and be useful to make an appropriate planning.