ABSTRACT
Aim To observe the influence of meisoindi-go on the alteration of Wnt/β-catenin signaling in type 1 diabetic rats ' myocardium and clarify its role in the development of diabetic cardiomyopathy .Methods The type 1 diabetes rat model was established by injec-tion of streptozocin after one-week adaptive feeding . The successful modeling rats were randomly divided in-to DM model group of 4 weeks and 8 weeks, meisoindi-go group of 4 weeks and 8 weeks.Fasting blood glu-cose(FBG) levels were tested.HE staining was used to observe the pathological changes of myocardial struc-tures.The alteration of GSK-3β, p-GSK-3β, Wnt2,β-catenin, NF-κB-p65, p-NF-κB-p65 in myocardium was determined by Western blot and immunohistochem-istry.Results Compared with control group , FBG levels of type 1 diabetic rats significantly increased ( P<0.01 ) , while body weight levels significantly de-creased ( P<0.01 ); compared with DM group , FBG levels of 8 weeks meisoindigo group significantly de-creased ( P <0.01 ) .Myocardial histological analysis revealed that DM induced myocardial focal myocyte hypertrophy , solubility , necrosis , fiber tissue hyperplasi-a; compared with DM group , these symptoms were eased in meisoindigo group of 4 weeks and 8 weeks. Compared with control group , the expression of p-GSK-3β, Wnt2, β-catenin, p-NF-κB-p65 level increased, especially with DM group of 8 weeks(P<0.01).The expression of p-GSK-3β, Wnt2,β-catenin, p-NF-κB-p65 level in meisoindigo group of 4 weeks and 8 weeks decreased significantly(P<0.01).Conclusions The repair effect of meisoindigo on myocardial damage in type 1 diabetic rats may be caused by lowering the ex-pression of proteins in Wnt/β-catenin signaling and in-hibiting the activation of Wnt/β-catenin signaling path-way, participating in the repair of myocardial damage and inflammatory in diabetic rats .Further researches on its mechanism in repairing diabetic myocardial dam-age may find new therapeutic targets for diabetic car-diomyopathy .