ABSTRACT
Objective To investigate the vasodilating effects of protocatechuic aldehyde (PCA ) on rat superior mesenteric arterial rings as well as its mechanism .Methods The tension of rat superior mesenteric arterial rings was recorded by a sensitive myograph system in vitro . We measured isometric tension changes in preconstricted rat superior mesenteric artery rings induced by potassium chloride (KCl ,60 mmol/L) ,serotomin (5-HT ,10-5 mol/L ) , and phenylephrine (PE , 10-5 mol/L ) after PCA treatment at different concentrations , respectively .We also observed vasodilating effects of PCA on KCl (60 mmol/L ) preconstricted rat superior mesenteric arterial rings after incubation with different inhibitors ,i .e .,L-NAME ,Indo ,ODQ ,4-AP (KV channel blocker) ,TEA (KCa channel blocker) ,BaCl2 (Kir channel blocker) ,and Glib (KATP channel blocker) ,respectively . Results PCA (10-6 -10-3 mol/L ) could relax KCl (60 mmol/L ) and 5-HT (10-5 mol/L ) preconstricted rat superior mesenteric arterial rings in a concentration-dependent manner . Indo of endothelial mechanism inhibitor blocked the vasodilating effect of PCA . 4-AP and BaCl2 of potassium ion channel inhibitors affected the vasodilatation induced by PCA in KCl (60 mmol/L)-preconstricted rat mesenteric artery .Conclusion PCA can relax KCl (60 mmol/L) ,or 5-HT preconstricted rat superior mesenteric arterial rings .This effect is associated with the inhibition of potassium channels and endothelial mechanism .
ABSTRACT
AIM To determine the contents of Pb,Cd,Hg,Cu and As in vinegar-processed,wine-processed,honey-processed and steamed products of Schisandrae chinensis Fructus and to investigate their speciations.METHODS After various elements were extracted by Tessier method,the contents of Pb and Cd were determined by graphite furnace atomic absorption spectrometry,and those of Cu,As and Hg were determined by flame atomic absorption spectrometry,hydride generation-atomic fluorescence spectrometry and cold vapour atomic absorption spectrometry,respectively.RESULTS Pb content was decreased in four kinds of processed products,while Hg content was increased in them.Cd content was only increased in wine-processed product.As was only detected in wine-processed product.Cu content showed no obvious change within standard limit.Pb speciations mainly existed in ion exchange and organic binding states in vinegar-processed and wine-processed products,Fe-Mn oxidative binding and organic binding states in honey-processed product,and carbonate binding and Fe-Mn oxidative binding state in steamed product.Hg,Cu and Cd speciations mainly existed in ion exchange and carbonate binding,ion exchange and organic binding,and carbonate binding states in four kinds of processed products,respectively.CONCLUSION Different processing methods show obvious effects on the contents and speciations of five elements in Schisandra chinensis Fructus.
ABSTRACT
AIM To determine the contents of Pb,Cd,Hg,Cu and As in vinegar-processed,wine-processed,honey-processed and steamed products of Schisandrae chinensis Fructus and to investigate their speciations.METHODS After various elements were extracted by Tessier method,the contents of Pb and Cd were determined by graphite furnace atomic absorption spectrometry,and those of Cu,As and Hg were determined by flame atomic absorption spectrometry,hydride generation-atomic fluorescence spectrometry and cold vapour atomic absorption spectrometry,respectively.RESULTS Pb content was decreased in four kinds of processed products,while Hg content was increased in them.Cd content was only increased in wine-processed product.As was only detected in wine-processed product.Cu content showed no obvious change within standard limit.Pb speciations mainly existed in ion exchange and organic binding states in vinegar-processed and wine-processed products,Fe-Mn oxidative binding and organic binding states in honey-processed product,and carbonate binding and Fe-Mn oxidative binding state in steamed product.Hg,Cu and Cd speciations mainly existed in ion exchange and carbonate binding,ion exchange and organic binding,and carbonate binding states in four kinds of processed products,respectively.CONCLUSION Different processing methods show obvious effects on the contents and speciations of five elements in Schisandra chinensis Fructus.