ABSTRACT
Objective To explore the clinical effect of minocycline hydrochloride ointment and iodine glycerol in the treatment of chronic periodontitis and the effect on the inflammatory cytokines in gingival crevicular fluid (GCF).Methods A total of 90 patients with chronic periodontitis (115 affected teeth) in Stomatology Hospital of Zhangjiakou from December 2015 to December 2016 were included and randomly divided into observation group(57 affected teeth) and control group (58 effected teeth),with 45 patients in each group.All patients were given routine periodental non-surgical treatment,based on this,the patients in the control group were smeared iodine glycerol (20 g · L-1) in the periodontal pocket with a probe;the patients in the observation group were injected minocycline hydrochloride ointment in the periodontal pocket until the ointment overflowed the gingival margin.The patients in the two groups were treated one time a week for 4 weeks.After 4-week treatment,the gingival index (GI),gingival sulcus bleeding index (SBI),probing depth (PD),plaque index (PLI),periodontal attachment level (AL) and the clinical efficacy were recorded.The levels of hypersensitive C-reactive protein(hs-CRP),interleukin8 (IL-8),interleukin-10 (IL-10),tumor necrosis factor-α (TNF-α),matrix metalloproteinases-8 (MMP-8) and soluble intercellular adhesion molecule-1 (sICAM-1) were detected by enzyme linked immunosorbent assay before and after treatment and compared between the two groups.Results There was no statistic difference in GI,SBI,PD,PLI,and AL between the two groups before treatment (P ＞ 0.05);the GI,SBI,PD,PLI,and AL after treatment were significantly lower than those before treatment (P ＜ 0.05);the GI,SBI,PD,PLI and AL in observation group were significantly lower than those in the control group after treatment (P ＜ 0.05).There was no statistic difference in the levels of hs-CRP,IL-8,IL-10,TNF-α,MMP-8 and sICAM-1 in GCF between the two groups before treatment(P ＞ 0.05);the levels of hs-CRP,IL-8,TNF-α,MMP-8 and sICAM-1 in GCF after treatment were significantly lower than those before treatment (P ＜ 0.05),but the IL-10 level was significantly higher than that before treatment in the two groups(P ＜ 0.05);the levels of hs-CRP,IL-8,MMP-8,TNF-α and sICAM-1 in GCF in observation group were significantly lower than those in the control group after treatment (P ＜ 0.05);the level of IL-10 was significantly higher than that in the control group after treatment (P ＜ 0.05).The total effective rate of affected teeth in observation group(96.49％,55/57) was significantly higher than that in the control group (74.14％,43/58) (x2 =11.40,P ＜ 0.05).Conclusion Minocycline hydrochloride ointment can effectively improve the inflammatory reaction status in patients with chronic periodontitis,and it can control the inflammatory damage of periodontal tissues by inhibiting the inflammatory reaction,and effectively alleviate the clinical symptoms.