The effect of platelet infusion on the repair of bone marrow hematopoietic niche damage in mice induced by (60)Co radiation and hematopoietic reconstruction after bone marrow transplantation / 中华血液学杂志

Objective: To observe the effect of platelets on hematopoietic stem cell (HSCs) implantation in mice with radiation-induced bone marrow injury and bone marrow transplantation models. Methods: ①Male C57BL/6 mice were divided into a single irradiation group and a radiation infusion group after receiving (60)Co semimyeloablative irradiation for 18-10 weeks. The irradiation infusion group received 1×10(8) platelets expressing GFP fluorescent protein. ② The allogeneic bone marrow transplantation model was established. The experimental groups included the simple transplantation group (BMT) and the transplantation infusion group (BMT+PLT). The BMT group was infused through the tail vein only 5 × 10(6) bone marrow cells, the BMT+PLT group needs to be infused with bone marrow cells at the same time 1× 10(8) platelets. ③ Test indicators included peripheral blood cell and bone marrow cell counts, flow cytometry to detect the proportion of hematopoietic stem cell (HSC) and hematopoietic progenitor cells, bone marrow cell proliferation and apoptosis, and pathological observation of vascular niche damage and repair. Results: ①On the 3rd, 7th, 14(th), and 21st days after irradiation, the bone marrow cell count of the infusion group was higher than that in the single irradiation group (P<0.05), and the peripheral blood cell count was also higher. A statistically significant difference was found between the white blood cell count on the 21st day and the platelet count on the 7th day (P<0.05). In the observation cycle, the percentage of bone marrow cell proliferation in the infusion group was higher, while the percentage of apoptosis was lower. ② The results of bone tissue immunofluorescence after irradiation showed that the continuity of hematopoietic niche with red fluorescence was better in the irradiation infusion group. ③The chimerism percentage in the BMT+PLT group was always higher than that in the BMT group after transplantation.④ The BMT+PLT group had higher bone marrow cell count and percentage of bone marrow cell proliferation on the 7th and 28th day after transplantation than that in the BMT group, and the percentage of bone marrow cell apoptosis on the 14th day was lower than that in the BMT group (P<0.05). After the 14th day, the percentage of stem progenitor cells in the bone marrow cells of mice was higher than that in the BMT group (P<0.05). ⑤The immunohistochemical results of bone marrow tissue showed that the continuity of vascular endothelium in the BMT+PLT group was better than that in the BMT group. Conclusion: Platelet transfusion can alleviate the injury of vascular niche, promotes HSC homing, and is beneficial to hematopoietic reconstruction.

Design and application of multifunctional patient suit / 医疗卫生装备

Objective To design a patient suit with simple structure,easy operation and multi function.Methods The suit was made of cotton and had L,M and S sizes.There were several openings and pockets at the jacket and trousers to hold the drainage tube,drainage bag,venous indwelling catheter and etc,so that the suit was gifted with multi function.Results The multifunctional suit protected the patient's privacy,avoided catheter from dropping off as well as the complications such as infection,and enhanced the patient's and nurse's satisfaction.Conclusion The suit is easy to manufacture and gains high comfort,and thus is worthy promoting for the patients of hemodialysis,peripherally inserted central venous catheter or femoral venous indwelling catheter.

Design and application of medical fixation plate / 医疗卫生装备

Objective To design a medical fixation plate with simple structure and easy operation which can be applied to hemodialysis and IV transfusion patients. Methods The plate was composed of a low-temperature thermoplastic board with semi-cylindric shape and net-like structure and an elastic fixation bandage, which was used for the treatment of hemodialysis and IV transfusion patients. Totally 40 hemodialysis patients including 22 males and 18 females from July to December 2016 were divided into an observation group and a control group randomly and equally. There were 400 IV transfusion patients including 188 males and 212 females enrolled into an observation group and a control group randomly and equally. The observation group applied the developed plate, and then compared with the control group on the rate for needle dropping off, the rate for needle displacement, nurse satisfaction and patient satisfaction. Results For the hemodialysis patients with 2020 times of punctures, there were no cases for needle dropping off and only 1 case of needle displacement due to improper fixation;the patient and nurse satisfaction (higher than 99%) in the observation group were obviously higher than that in the control group (P<0.001). For the IV transfusion patients, there were no cases of needle dropping off and 2 cases of needle displacement due to the patients' abusing of the developed plate;the patient and nurse satisfaction in the observation group was obviously higher than that in the control group (P<0.05). Conclusion The plate fixes the limbs of the hemodialysis and IV transfusion patients efficiently, decreases the incidences of needle dropping off and displacement, reduces the workload of medical staffs, enhances the patient and nurse satisfaction, and thus is worthy promoting practically.