ABSTRACT
BACKGROUND: Surgically assisted rapid maxillary expansion (SARME) has been proved to exert good therapeutic efficacy on maxillary transverse deficiency (MTD) in adults, but its long-term efficacy and stability still remain controversial.OBJECTIVE: To analyze the stability improvement in adult MTD after SARME through a Meta-analysis.METHODS: A computer-based search of PubMed, Medline, EMBASE, Central Register of Controlled Trials, CNKI, and WanFang databases was conducted for the randomized, quasi-randomized controlled trials and clinical case-control trials published before May 31st, 2015. The quality evaluation and data extraction in the middle- and high-quality literature were performed by two professional researchers independently, and a Meta-analysis was then performed using Revman5.3 software.RESULTS AND CONCLUSION: Totally 13 articles involving 513 cases were included. The results of Meta-analysis showed that: (1) the stability of the teeth: the difference of the first molar (WMD=-1.03, 95%CI: -2.00--0.06), canine (WMD=-1.26, 95%CI: -2.02 to -0.49) had significant difference after surgery and the follow-up; (2) the stability of the bone: the maxillary alveolar width of the first molar (WMD=-0.43, 95%CI: -1.82 to 0.97) at post-operation and the follow-up had no significant difference; (3) the stability of the nasal airway: in the short-term, the function of the nasal airway was improved significantly, while the long-term efficacy needed to be confirmed further. To conclude, after SARME, the dental and the maxillary alveolar in adult MTD have kept long-term stability, and the nasal airway is improved significantly in short-term.
ABSTRACT
Objective SCAP are seen as seed cells of peri-apical tissue regeneration and used in periapical tissue regeneration project based on stem cells .In this study, we aim to explore the ef-fectdifferent mechanical stretch stress on the proliferation and differen-tiation potential of human stem cells from the apical papilla ( SCAP ) ,and to clarify the mechanism of how mechanical stretch stress regulate human SCAP ,which will provide theoretical guidance for ortho-dontic treatment . Methods Human SCAP was isolated , cultured and identified by combined explants method and enzymatic separa-tion method and limited dilution .MTT assay was used to detect the effect different static mechanical stretch stress stimulation have on the proliferation of SCAP .Western blot was used to detect the expression changes of SCAP osteogenesis /odontoblast differentiation-re-lated protein (ALP, OSX,DSP) under mechanical stretch stressand to detect the expression of SCAP endoplasmic reticulum stress mo -lecular chaperone GRP 78 under different static mechanical stretch stress . Results SCAP were successfully isolated and cultured , and we induced SCAP to differentiate into osteoblasts and adipocytes successfully by osteogenic medium and adipogenic medium .Flow cytometry was performed in accordance with SCAP immunophenotype .Compared with the control group , 150g mechanical stretch stress stimulation promoted SCAP proliferation first and then inhibited SCAP proliferation [(0.481±0.226),(1.375±0.104),(1.425± 0.136),(1.556±0.268),(0.589±0.29),P<0.05].It was same in the 200g group.250g mechanical stretch stress stimulation signifi-cantly inhibited SCAP proliferation [(0.373±0.146),(0.545±0.069),(0.745±0.273),(0.967±0.278),(1.060±0.362),P<0.05]. The expression levels of ALP , OSX and DSP protein in each group were higher than those in the control group (P<0.05).Compared with the control group, the expression of GRP78 protein was up-regulated (P<0.05). Conclusion Mechanical stretch stress could regulate the SCAP proliferation and osteogenesis/odontoblast differentiation .What′s more,endoplasmic reticulum stress played a role in osteogenesis/odontoblast differentiation under mechanical stretch stress and promoted SCAP osteogenesis /odontoblast differentiation .
ABSTRACT
Objective SCAP are seen as seed cells of peri-apical tissue regeneration and used in periapical tissue regeneration project based on stem cells .In this study, we aim to explore the ef-fectdifferent mechanical stretch stress on the proliferation and differen-tiation potential of human stem cells from the apical papilla ( SCAP ) ,and to clarify the mechanism of how mechanical stretch stress regulate human SCAP ,which will provide theoretical guidance for ortho-dontic treatment . Methods Human SCAP was isolated , cultured and identified by combined explants method and enzymatic separa-tion method and limited dilution .MTT assay was used to detect the effect different static mechanical stretch stress stimulation have on the proliferation of SCAP .Western blot was used to detect the expression changes of SCAP osteogenesis /odontoblast differentiation-re-lated protein (ALP, OSX,DSP) under mechanical stretch stressand to detect the expression of SCAP endoplasmic reticulum stress mo -lecular chaperone GRP 78 under different static mechanical stretch stress . Results SCAP were successfully isolated and cultured , and we induced SCAP to differentiate into osteoblasts and adipocytes successfully by osteogenic medium and adipogenic medium .Flow cytometry was performed in accordance with SCAP immunophenotype .Compared with the control group , 150g mechanical stretch stress stimulation promoted SCAP proliferation first and then inhibited SCAP proliferation [(0.481±0.226),(1.375±0.104),(1.425± 0.136),(1.556±0.268),(0.589±0.29),P<0.05].It was same in the 200g group.250g mechanical stretch stress stimulation signifi-cantly inhibited SCAP proliferation [(0.373±0.146),(0.545±0.069),(0.745±0.273),(0.967±0.278),(1.060±0.362),P<0.05]. The expression levels of ALP , OSX and DSP protein in each group were higher than those in the control group (P<0.05).Compared with the control group, the expression of GRP78 protein was up-regulated (P<0.05). Conclusion Mechanical stretch stress could regulate the SCAP proliferation and osteogenesis/odontoblast differentiation .What′s more,endoplasmic reticulum stress played a role in osteogenesis/odontoblast differentiation under mechanical stretch stress and promoted SCAP osteogenesis /odontoblast differentiation .