ABSTRACT
AIM To evaluate the quality of fifteen varieties of Epimedii Folium from different growing areas.METHODS UPLC-MS/MS was adopted in the content determination of icariin,icariside Ⅰ,icariside Ⅱ,epimedin A,epimedin B,epimedin C,sagittatoside A,sagittatoside B,2"-O-rhamnosylicariside Ⅱ and baohuoside Ⅱ.The analysis of 40％ ethanol extract of Epimedii Folium was performed on a 40 ℃ thermostatic Welch C18 column (50 mm × 2.1 mm,3 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner.Then principal component analysis was made.RESULTS Ten constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 97.6％-104.5％ with the RSDs of 1.37％-2.93％.In 46 batches of samples,epimedin C content was the highest,followed by epimedin A content,and icariside Ⅰ content was the lowest.The contents of various constituents showed obvious differences between small-flowered taxa and big-flowered taxa.CONCLUSION This sensitive and rapid method can provide a reference for the development and utilization of Epimedii Folium.
ABSTRACT
AIM To evaluate the quality of fifteen varieties of Epimedii Folium from different growing areas.METHODS UPLC-MS/MS was adopted in the content determination of icariin,icariside Ⅰ,icariside Ⅱ,epimedin A,epimedin B,epimedin C,sagittatoside A,sagittatoside B,2"-O-rhamnosylicariside Ⅱ and baohuoside Ⅱ.The analysis of 40％ ethanol extract of Epimedii Folium was performed on a 40 ℃ thermostatic Welch C18 column (50 mm × 2.1 mm,3 μm),with the mobile phase comprising of 0.1％ formic acid-acetonitrile flowing at 0.2 mL/min in a gradient elution manner.Then principal component analysis was made.RESULTS Ten constituents showed good linear relationships within their own ranges (r ＞ 0.999 0),whose average recoveries were 97.6％-104.5％ with the RSDs of 1.37％-2.93％.In 46 batches of samples,epimedin C content was the highest,followed by epimedin A content,and icariside Ⅰ content was the lowest.The contents of various constituents showed obvious differences between small-flowered taxa and big-flowered taxa.CONCLUSION This sensitive and rapid method can provide a reference for the development and utilization of Epimedii Folium.