ABSTRACT
Objective To investigate the effects of saponins from Allium Macrostemon Bunge Bulbs (SMBB) on platelet aggregation and platelet-neutrophil-interactions. Methods The effects of SMBB on platelet aggregation in SD rats were observed in vivo and in vitro. The adhesion of platelets to neutrophils was measured by rosette test. The effect of SMBB on activated platelet calcium levels was detected. Results Platelet aggregation induced by platelet activating factor (PAF), adenosine diphosphate (ADP) and arachidonic acid (AA) was significantly inhibited by SMBB in vitro concentration-dependent. Platelet aggregation induced by PAF, AA and ADP was significantly inhibited by 5 mg/kg of SMBB. SMBB could reduce the intracellular calcium concentration in the wash platelets. SMBB significantly reduced the adhesion between neutrophils and thrombin-activated platelets and inhibited neutrophil supernatant-induced platelet aggregation, with IC50of 2.7 μmol/L and 9.6 μmol/L, respectively. Conclusion SMBB can inhibit platelet aggregation in vitro and ex vivo, and inhibit the interactions between platelets and neutrophils.