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This paper reviews the new progress in the research of fractional carbon dioxide laser in treating hypertrophic scar after burn injury, which remains a challenging problem for burn care surgeons. There have been many traditional therapeutic approaches, such as compression remedy, operation, and so on. However, a satisfactory method is lacking to date. In recent years, the newly developed fractional carbon dioxide laser has been employed to treat different kinds of scars, and it has been proved to be effective in terms of an improvement of scar color, texture, and rigidity. It seems to be a promising method for scar treatment in future.
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Humans , Burns , General Surgery , Cicatrix , General Surgery , Cicatrix, Hypertrophic , General Surgery , Lasers, Gas , Treatment OutcomeABSTRACT
Objective To construct the lentivirus carrying human ?-catenin-EGFP(enhanced green fluorescent protein)and observe its expression in human follicle stem cells.Methods The ?-catenin gene sequence was amplified by RT-PCR from extraction of total RNA of human vascular endothelial cells.TA cloning technique was utilized to acquire gene subcloned pUCm-T-?-catenin.After transformation reaction,candidate clone was further analyzed by PCR and gene sequencing.Then the plasmid was transfected into FT293 cells.After identification by Western blotting,the plasmid was transfected into FT293 cells again for packaging.Infection titer was monitored by green EGFP expression.The expression of ?-catenin-lentivirus in human follicle stem cells were observed under inverted fluorescence microscope.Results The ?-catenin gene was cloned into the lentivirus successfully.The high expression of green fluorescence protein in FT293 cell line was found under fluorescent microscope.Viral titer checked by real-time PCR was about 2.0?108 TU/mL.When the multiplicity of infection(MOI)was 10,the infection efficiency of ?-catenin-lentivirus in human follicle stem cells was nearly 80% after infection 48 h around.After 3 weeks of continuous observation,we found the infection efficiency still keeping in the range of 80%-90%.Conclusion The lentivirus expression vector for ?-catenin was successfully constructed.It can steadily infect human follicle stem cells and the infection efficiency is considerable high.
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0.05).The protein and mRNA expression of MIP-2 in high glucose group significantly increased after culture for 4 h,and guadually decreased then.The protein and mRNA expression of MCP-1 began to increase significantly after culture for 8 h,reached peak at 12 h,and slightly decreased after culture for 24 and 48 h. Conclusion High glucose promotes the protein and mRNA expression of MIP-2 and MCP-1 from mouse peritoneal macrophages cultured in vitro,which indicates that high glucose may delay the wound healing by increasing the expression of chemokines in diabetic mice.
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21 d groups,respectively. Conclusion IL-8 plays an important role in burn wound healing as a chemotactic factor.
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Objective To detect the concentrations of (soluble TNF receptor type,sTNFR-I, sTNFR-II) and (soluble vascular cell adhesion molecule-1,sVCAM-I) in deep burn wound fluids and the number of macrophages in different stage of human deep burn wound, and to analyse the correlation between them. Methods The wound fluid was collected with sponge during the dressing change, and then the concentrations of sTNFR-I, sTNFR-II and sVCAM-I were detected by ELISA. The tissues of burn wound were collected at operation, and the tissue slices were dyed with HE.The macrophages were marked by immunohistochemistry with antibody of CD68, and then the number of macrophages was counted under the microscope. Results The concentrations of sTNFR-I and sTNFR-II in burn wound fluid and the number of macrophages in burn wound were significantly increased two days postburn, which maintained at high levels until the wound healed. The concentration of sVCAM-I was decreased immediately after the burn until the wound healed. The correlation coefficient between the concentrations of sTNFR-I, sTNFR-II, sVCAM-I and the number of macrophages were 0.95, 0.97 and -0.37, respectively. Conclusion The number of macrophages has a strong correlation with sTNFR-I and sTNFR-II in human deep burn wound, and has a weak correlation with sVCAM-I,which suggests that the macrophages may play a cooperative role with sTNFR in burn wound healing.