ABSTRACT
Objective To investigate the effects of chlorogenic acid (CGA) at high concentration ( > 100 μmol/L) on the lipid accumulation and oxidative stress in L02 cells under normal and non alcoholic liver disease (NAFLD) status induced by oleic acid (OA) and palmic acid (PA) treatment. Methods L02 cells were treatment by OA (666 μmol/L)-PA (333 μmol/L) for 24 h to induce intracellular steatosis. After normal and OA-PA treated cells were treated by CGA (0, 0.5, 1, and 2 mmol/L) for 24 h, the intracellular contents of lipid droplets and reactive oxygen species (ROS) were determined by Oid red staining and fluorospectrophotometry, respectively. And the mRNA and protein expression levels of SREBP-1C, PNPLA3, and CYP2E1 were detected by Real-time PCR and Western blotting, respectively. Results CGA treatment dose-dependently increased the levels of intracellular lipid droplets and ROS, as well as the mRNA and protein expression levels of SREBP-1C, PNPLA3, and CYP2E1 in normal and OA-PA treated L02 cells. Conclusion CGA treatment at high concentration can accelerate the lipid accumulation and oxidative stress injury in L02 cells under normal and NAFLD status.
ABSTRACT
BACKGROUND: To solve the dislocation following hip arthroplasty, a double mobility acetabular prosthesis has been developed, and its effect still needs to be confirmed through follow-up observation.OBJECTIVE: To explore the early clinical effectiveness of total hip replacement with double mobility acetabular prosthesis.METHODS: Thirty patients admitted from January 2013 to November 2015, were given total hip replacement with double mobility acetabular prosthesis, and were then followed up for 1 year. The hip function and loosening were evaluated by Harris hip scores, Beijing program scaling and imaging examination.RESULTS AND CONCLUSION: (1) All patients were followed up for 1 year. (2) The mean Harris hip score was significantly increased from preoperatively (39.7±3.87) to postoperatively (93.6±3.82), and the mean score of the Beijing program scaling was also significantly increased from (10.5±2.46) to (17.3±1.87) (both P < 0.05). (3) All patients suffered from moderate to severe pain before replacement, and 28 patients complained mild or no pain and 2 patients with moderate pain after operation. X-ray showed a stable hip. (4) To conclude, the total hip arthroplasty with double mobility acetabular prosthesis achieves satisfactory short-term treatment outcomes, but its long-term effect needs to be observed further.