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Article | IMSEAR | ID: sea-187995


Background: Goat milk is recognized for its high nutritive profile. The practise of antimicrobials in feeding of animals produces resistance in bacteria. Therefore, the present study was proposed to study the incidence of drug-resistant E. coli from raw goat milk samples and investigate the genes responsible for the resistance. Methods: A total of 250 raw milk samples were obtained from different farms of Taif province, Saudi Arabia. Collected samples were cultured on MacConkey agar. Morphological and biochemical tests were achieved for the identification of isolates. Antimicrobial resistance pattern of E. coli was estimated by the disk diffusion method. The resistance genes tet(A) and tet(B), ere(A), aadA1, blaSHV, aac(3)-IV, sul1, catA1 and cmlA, were examined by PCR. Results: Results of the present study showed that out of the 250 samples examined, 100 (40%) were found to be infected with E. coli. Antimicrobial resistance profile evaluated showed a higher resistance against ceftriaxone (90 %) and ticarcillin (86%), followed by amikacin and cefotaxime (87%), and augmentin and penicillin (85%). Lower percentage was observed for gentamicin (58%), ampicillin (66%), bacitracin (75%) and imipenem (32%). Furthermore, multi-drug resistance was observed in most of the isolates. Among E. coli isolates, 86% gave positive amplicons for the blaSHV gene followed by tet(A) and tet(B) genes (85%). Conclusion: The results suggested a probability of possible public health risk of multi-drug resistance of E. coli strains collecting from raw goat milk samples. Consequently, appropriate handling of goat milk processing is significant to prevent E. coli infection.

Braz. j. microbiol ; 45(4): 1199-1209, Oct.-Dec. 2014. ilus, graf, tab
Article in English | LILACS | ID: lil-741269


Emergence and distribution of multi-drug resistant (MDR) bacteria in environments pose a risk to human and animal health. A total of 82 isolates of Escherichia spp. were recovered from cloacal swabs of migrating and non-migrating wild birds. All bacterial isolates were identified and characterized morphologically and biochemically. 72% and 50% of isolates recovered from non-migrating and migrating birds, respectively, showed positive congo red dye binding (a virulence factor). Also, hemolysin production (a virulence factor) was showed in 8% of isolates recovered from non-migrating birds and 75% of isolates recovered from migrating birds. All isolates recovered from non-migrating birds were found resistant to Oxacillin while all isolates recovered from migrating birds demonstrated resistance to Oxacillin, Chloramphenicol, Oxytetracycline and Lincomycin. Some bacterial isolates recovered from non-migrating birds and migrating birds exhibited MDR phenotype. The MDR isolates were further characterized by API 20E and 16S rRNA as E. coli and E. vulneris. MDR Escherichia isolates contain ~1-5 plasmids of high-molecular weights. Accordingly, wild birds could create a potential threat to human and animal health by transmitting MDR bacteria to water streams and other environmental sources through their faecal residues, and to remote regions by migration.

Animals , Anti-Bacterial Agents/pharmacology , Carrier State/veterinary , Drug Resistance, Multiple, Bacterial , Enterobacteriaceae Infections/veterinary , Escherichia/drug effects , Escherichia/isolation & purification , Bacterial Typing Techniques , Birds , Cluster Analysis , Carrier State/microbiology , Cloaca/microbiology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Enterobacteriaceae Infections/microbiology , Escherichia/classification , Molecular Sequence Data , Phylogeny , /genetics , Sequence Analysis, DNA , Virulence Factors/analysis