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Objective:To investigate the changes in γ-aminobutyric acid (GABA) receptor currents of hippocampal dentate gyrus granule cells in prepubertal and early pubertal female mice.Methods:Female mice were selected as the study objects; 3 to 4 -week-old mice were selected as the pre-puberty group ( n=6); and 5 to 6 -week-old mice were selected as the puberty group ( n=6). The whole-cell patch clamp technique was used to record the spontaneous inhibitory post-synaptic current (sIPSC), mini-inhibitory post-synaptic current (mIPSC), and tonic current of hippocampal granulosa cells in the DG region of pre-pubertal and early pubertal female mice, and their changes were analyzed. Results:The frequency of sIPSC in the pre-puberty group and puberty group was (2.22 ± 0.12) Hz and (2.30 ± 0.21) Hz, respectively. The amplitude of sIPSC in the pre-puberty group and the puberty group was (19.97 ± 2.01) pA and (23.80 ± 2.86) pA, respectively. The experimental results showed no significant changes in frequency and amplitude of sIPSC of hippocampal granulosa cells in pre-pubertal and early pubertal mice (all P > 0.05), and no statistical significance in the cumulative frequency and amplitude of sIPSC between two groups (all P > 0.05). The frequency of mIPSC in the pre-puberty group and the puberty group was (0.87 ± 0.08) Hz and (2.15 ± 0.21) Hz, respectively. The amplitude of mIPSC in the pre-puberty group and puberty group was (12.51 ± 0.11) pA and (29.67 ± 0.19) pA, respectively. Compared with the pre-pubertal mice, the frequency and amplitude of mIPSC from hippocampal granulosa cells in early pubertal mice are significantly increased ( P < 0.001). There was also a significant difference in the cumulative frequency and amplitude of sIPSC between the two groups ( P < 0.001). The tonic current of the pre-puberty group and puberty group was (17.40 ± 1.64) pA and (24.70 ± 2.81) pA, respectively, and the tonic current in early pubertal mice was significantly higher than that in pre-pubertal mice ( P < 0.05). Conclusions:GABA receptor current is enhanced in early pubertal female mice compared with pre-pubertal females. The inhibitory activity of hippocampal granulosa cells in early adolescent female mice was increased.
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Objective@#To analyze the association between fat mass percentage (FMP) and waist circumference (WC) with cardiovascular risk factors in children and adolescents in Yinchuan City, and to provide a reference for preventing and contyolling the risk factors of cardiovascular diseases among children and adolescents.@*Methods@#A questionnaire survey, physical examination, body composition determination and biochemical tests were conducted among 1 043 children aged 12-18 years in Yinchuan selected through a convenient cluster sampling method.@*Results@#Among boys and girls, both FMP and WC were associated with high rate of high blood pressure and high TG in children and adolescents(P<0.01). The increased risk of metabolic abnormalities by FMP and WC were 1.14-2.36 times and 1.32-2.09 times, respectively. About 4.9%-22.1% of cardiovascular disease risk was explained by the combination of FMP and WC, which was significantly higher than separate interpretation of FMP or WC (3.5%-22.0%). Standardized regression coefficient for TC and LDL-C (0.25 and 0.22) was greater than WC (0.17 and 0.14) by FMP, and the absolute value of the standardized regression coefficient for SBP, DBP, TG, HDL-C was less than WC by FMP. The predictive risk for high blood pressure, high TG, high TC, low HDL-C and high LDL-C by combination of FMP and WC was 3.67(95%CI=2.42-556), 3.09(95%CI=2.04-4.69), 3.37(95%CI=1.38-8.28), 2.00(95%CI=1.35-2.98) and 4.73(95%CI=2.15-10.44) times higher than different combinations risk groups(P<0.01).@*Conclusion@#The ability of fat mass percentage and waist circumference to predict the risk of cardiovascular risk factors is similar. It might be used as a predictor of cardiovascular risk factors in children and adolescents. It is recommended to combine FMP with WC to predict the risk of cardiovascular disease among children and adolescents.
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Objective To study the effect of network peer education on the 131I treatment adherence in patients with thyroid cancer.Methods A total of 120 patients with thyroid cancer who performing 131 I treatment from April 2012 to April 2014.They were divided into intervention group 61 cases and control group 59 cases according to the hospital ward number.Control group was given routine health education and intervention group was given network peer education.The data from the habits,drug therapy,grasp the situation,the equivalent monitoring of the relevant knowledge of 4 different dimensions of treatment adherence for patients were investigated and compared before discharge by using questionnaire and examination method.Results The incidence of the good habits,drug therapy,grasp the situation,and the equivalent monitoring reaching the standard were 96.7％(59/61),98.4％(60/61),96.7％(59/61),100.0％(61/61) in intervention group,and 54.2％(32/59),76.3％(45/59),79.7％(47/59),89.8％(53/59) in control group,there were significant differences,x2=29.54,13.38,8.47,4.56,P＜0.01 or ＜0.05.Conclusion Network peer education for patients with thyroid cancer during the 131I treatment can effectively improve the patient's treatment adherence,has positive significance for the treatment.
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<p><b>OBJECTIVE</b>To establish a quantitative HPLC method for determination of protopine, palmatine hydrochloride, bicuculline and tetrahydropalmatine, in Corydalis decumbens.</p><p><b>METHOD</b>The separation was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) at a flow rate of 1.0 mL x min(-1) using mixtures of two solvents [A(20 mmol x L(-1) ammonium acetate)-B(acetonitrile)]: with a gradient elution. The column oven temperature was 30 degrees C and the detection wavelength was set at 280 nm.</p><p><b>RESULT</b>The 4 alkaloids were well separated by this HPLC method. Linearifies of protopine, palmatine hydrochloride, bicuculline and tetrahydropalmatine were good in the ranges of 1.44-46.0 (r = 0.999 4), 1.2640.2 (r = 0.999 8), 1.37-44.0 (r = 0.999 9), and 1.3643.6 mg x L(-1) (r = 0.999 9), respectively. The average recoveries were 98.2% with RSD 2.7% for protopine, 101.9% with RSD 2.5% for palmatine hydrochloride, 102.8% with RSD 3.5% for tetrahydropalmatine, and 98.8% with RSD 3.1% for tetrahydropalmatine.</p><p><b>CONCLUSION</b>This method is proved to be convenient, reliable and accurate., and it can be used for quality control of C. decumbens.</p>
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Alkaloids , Chromatography, High Pressure Liquid , Corydalis , Chemistry , Drug Stability , Plants, Medicinal , Chemistry , Reproducibility of ResultsABSTRACT
Objective To understand the difference between psychological characteristics and stress response as a result of military stress and to evaluate the rate of stress response among individuals with different psychological characteristics.Methods The State-Trait Anxiety Inventory,blood pressure,breath,pulse wave,and serum levels of lactate acid and cortisol were measured at 8 to 9 am for 372 servicemen following 15 days'training.ResultsIn 97 subjects ,the value of special characteristic anxiety Was 2-standard deviation higher than normal model(i.e.high special characteristic anxiety).and low special characteristic anxiety was found in 79.The condition anxiety of 2 standard deviation higher than normal model wag found in 172 participants.A total of 132 subjects showed significant changes in condition anxiety,serum cortisol and lactic acid,and systolic blood pressure(P＜0.01).Sixty-one or 71 subjects with high or low special characteristic anxiety experienced condition anxiety,changed serum cortisol and lactic acid,and systolic blood pressure,respectively.Conclusion Psychological characteristics may be considered as a predicting factor of stress response.
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Stressors can be classified as controllable and uncontrollable events.Self-choice behavior plays an important role in the "problem-solution" process of coping when individual faces controllable stressors.As to the uncontrollable stressors there are 2 different coping modes: transformation and compensation coping.This paper reviews the research achievements in the relationship between stress and self-choice behavior.
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BUMwith high insulin level.