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1.
Article in Chinese | WPRIM | ID: wpr-746619

ABSTRACT

OBJECTIVE@#To clarify the association between regulatory region of HLA-DPB1 (3'UTR) with Naso pharyngeal carcinoma in Guangdong Province Hans.@*METHOD@#The allelic types of HLA-DPB1-3'UTR were detected by sequence specific primer (SSP) in 104 patients with NPC and 105 control individuals from Guangdong Province Hans.@*RESULT@#The frequencies of allelic types B/B, haplotype B were higher in patients with NPC than those of the control individuals.@*CONCLUSION@#Positive association may exist between certain HLA-DPB1 alleles and NPC in Guangdong Province Hans.


Subject(s)
Alleles , Asians , Genetics , Base Sequence , Case-Control Studies , Gene Frequency , HLA-DP Antigens , Genetics , HLA-DP beta-Chains , Haplotypes , Humans , Molecular Sequence Data , Nasopharyngeal Neoplasms , Genetics , Polymorphism, Single Nucleotide
2.
Chinese Medical Journal ; (24): 827-830, 2003.
Article in English | WPRIM | ID: wpr-294222

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the changes of subgroups of peripheral blood T lymphocytes in patients with severe acute respiratory syndrome (SARS) and its clinical significance.</p><p><b>METHODS</b>Subgroups of blood T lymphocytes in 93 patients with SARS were detected by flow cytometer. The results detected in 64 normal subjects and 50 patients with AIDS served as controls.</p><p><b>RESULTS</b>The numbers of CD(3)(+), CD(4)(+), and CD(8)(+) lymphocytes all significantly decreased in acute phase of patients with SARS [(722 +/- 533)/microliter, (438 +/- 353)/microliter, (307 +/- 217)/microliter] compared with those in normal controls [(1527 +/- 470)/microliter, (787 +/- 257)/microliter, (633 +/- 280)/microliter, all P <0.01], which was different from what we observed in patients with AIDS who had decreased CD(4)(+) [(296 +/- 298)/microliter] but increased CD(8)(+) [(818 +/- 566)/microliter] counts. The counts of CD(3)(+), CD(4)(+), and CD(8)(+) lymphocytes decreased more apparently in patients with severe SARS. All the five patients who died had CD(4)(+) counts less than 200/microliter. As the patients' condition improved, CD(3)(+), CD(4)(+), and CD(8)(+) counts gradually returned to normal ranges.</p><p><b>CONCLUSION</b>The damage of cellular immunity is probably an important mechanism of pathogenesis of SARS.</p>


Subject(s)
Acquired Immunodeficiency Syndrome , Allergy and Immunology , Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Leukocyte Count , Male , Middle Aged , Severe Acute Respiratory Syndrome , Allergy and Immunology , T-Lymphocyte Subsets , Allergy and Immunology
3.
Article in Chinese | WPRIM | ID: wpr-245366

ABSTRACT

<p><b>OBJECTIVE</b>To make a comparative study of HLA-DQA1 and HLA-DRB1 allele frequencies in the cases of endometriosis and adeonmyosis.</p><p><b>METHODS</b>The allelic types of HLA-DQA1 and HLA-DRB1 were detected by polymerase chain reaction-sequence specific primers (PCR-SSP) technique in 51 cases of endometriosis, 45 cases of adenomyosis, and 44 normal individuals as the control.</p><p><b>RESULTS</b>The frequencies of HLA-DQA1*0401(7.8%, 10.0%) were significantly increased in the endometriosis group and the adenomyosis group (Pc=0.03, Pc=0.01), and the frequencies of HLA-DQA1*0301(8.8%, 5.6%) were significantly decreased in these two groups (Pc=0.00, Pc=0.00).There was no significant difference between the frequencies of HLA-DQA1 and HLA-DRB1 of endometriosis and adenomyosis.</p><p><b>CONCLUSION</b>The results indicate that HLA-DQA1*0301 and *0401 alleles are associated with both endometriosis and adenomyosis, and there is perhaps common mechanism involved in both endometriosis and adenomyosis based on HLA-DQA1 and HLA-DRB1 allele frequencies.</p>


Subject(s)
Asians , Genetics , Endometriosis , Genetics , Female , Gene Frequency , Genetic Predisposition to Disease , Genotype , HLA-DQ Antigens , Genetics , HLA-DQ alpha-Chains , HLA-DR Antigens , Genetics , HLA-DRB1 Chains , Humans , Polymerase Chain Reaction , Methods
4.
Article in Chinese | WPRIM | ID: wpr-675359

ABSTRACT

Objective Vessel injury provokes the release of angiotensin II that influences the proliferation and migration of vascular smooth muscle cells (VSMCs). T lymphocytes produced, interleukin 10 is of immunoregulatory function. The purpose of this study is to determine the effects of recombinant human interleukin 10 (rhIL 10) on the proliferation of isolated rat vascular smooth muscle cell and the activity of p44/p42 mitogen activated protein kinase (MAPK) promoted by angiotensin II. Methods Rat aortic VSMCs were cultured and treated with rhIL 10 or angiotensin II, and then co treated with rhIL 10 and angiotensin II. The proliferation was quantified by colormetric assay. The p44/p42 MAPK activity was evaluated by the immunobloting technique using anti p44/p42 phospho MAPK antibody. Results Compared with control group, angiotensin II stimulated significantly VSMCs proliferation(1 311?0 201 vs 0 781?0 236, P 0 05), but significantly inhibited VSMCs proliferation induced by angiotensin II at a dose of 1,10,100 ng/ml (0 984?0 172 , 0 932?0 134 , 0 784?0 097 vs 1 311?0 201, P

5.
Article in Chinese | WPRIM | ID: wpr-683918

ABSTRACT

By making investigation on the animals with venom protein and peptied.In China sea we find that Coelenterata of them mainly includes 14 kinds of Jellyfist,15 kinds of Sea anemone,one kind of Corals and two kinds of Hydrozoa,altogether 32 kinds;Mollusea of them mainly includes 34 kinds of Conus,two kinds of Aplysia and 3 kinds of Cephalopoda,altogether 39 kinds;Nemartina of them has one kind;Echinodermata of them mainly includes two kinds of starfish and 8 kinds of Sea urchins,altogether 10 kinds;fishes of them mainly include one kind of Venomous fishes,two kinds of Ichthyocrinotoxic fishes and 74 kinds of Venomous fishes,altogether 77 kinds;Sea snake is 15 kinds.These animals with venom protein and peptide mainly are distributed in South sea of China.

6.
Article in Chinese | WPRIM | ID: wpr-520141

ABSTRACT

AIM: To determine the effects of recombinant human interleukin-10(rhIL-10) on proliferation of vascular smooth muscle cells(VSMC) by TNF-? and PDGF-BB and neointimal hyperplasia after rat carotid arterial injury.METHODS: Rat aortic VSMC was cultured and treated with rhIL-10 with or without tumor necrosis factor-?(TNF-?) and platelet-derived growth factor-BB (PDGF-BB), respectively.Proliferation of VSMC was quantified by colormetric assay.Cell cycle analysis was performed by flow cytomertry.SD rats were treated with recombinant human IL-10(rhIL-10) for 3 days after carotid arteries injury.Neointima to media area ratio at the site of arterial injury was measured at 28 days after balloon injury.RESULTS: Compared to control,both TNF-? and PDGF-BB stimulated VSMC proliferation. rhIL-10 alone had no effect on VSMC growth.With TNF-? or PDGF-BB stimulation,rhIL-10,at dose as low as 10 ?g/L,inhibited VSMC growth( P

7.
Article in Chinese | WPRIM | ID: wpr-519520

ABSTRACT

AIM: To investigate the association between HBV infection and HLA-DPB1 gene in population of Guangzhou Chinese. METHODS: 58 unrelated patients (test positive of HbsAg,HBeAg,HbcAb) and 75 unrelated healthy control individuals were typed by sequencing based typing (SBT) method in their HLA-DPB1 gene. RESULTS: The phenotype frequencies of HLA-DPB1 alleles of patients and control have no significant difference. CONCLUSION: These results indicate that there is no association between HLA-DPB1 gene and HBV infection.

8.
Article in Chinese | WPRIM | ID: wpr-541581

ABSTRACT

Objective:To study the HLA-DPB1 allele polymorphism in Ewenki from Inner Mongolian.Methods:HLA-DPB1 allele polymorphism in normal Ewenki were determined by PCR with sequencing-based-typing(SBT).Results:20 HLA-DPB1 alleles were observed and compared with other ethnic groups,the allele frequency of HLA-DPB1*02012(24.4%) and DPB1*0402(22.6%),DPB1*0401(20.2%),DPB1*0501(10.7%) are highest,while others are lower.Conclusion:The distributions of HLA-DPB1 alleles frequencies in normal Ewenki from Inner Mongolia has a unique style.It is most important to further study anthropology and related to illness in Ewenki nationality.

9.
Article in Chinese | WPRIM | ID: wpr-570788

ABSTRACT

Object The purpose of this study was to determine the effects of tea polyphenols on rat vascular smooth muscle cells (VSMCs) proliferation. Methods Rat aortic VSMCs were cultured and treated with tea polyphenols for 24 h. Cell proliferation was quantified by colormetric assay and definited by MTS/PES. Cell cycle analysis was performed by flow cytometry. Results Compared with the control group, VSMCs growth was significantly inhibited by tea polyphenols (P

10.
Article in Chinese | WPRIM | ID: wpr-682253

ABSTRACT

Object To study the chemical constituents of cytotoxicity portion in the bark of Reevesia longipetiolata Merr et Chun Methods The constituents were isolated and purified by various chromatographic methods, such as gel column chromatography under normal pressure and increased pressure, Sephadex LH 20 column chromatography and HPLC, and the structures were identified by physicochemical properties and spectral analysis Results Five compounds were obtained in the ethyl acetate fractions and identified as ? sitosterol (Ⅰ), daucosterol (Ⅱ), betulinic acid (Ⅲ), lupeol (Ⅳ) and (+) catechin (Ⅴ) Conclusion All above compounds are obtained from the plants of Reevesia Lindl for the first time, and their cytotoxicity is discussed

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