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National Journal of Andrology ; (12): 110-119, 2017.
Article in Chinese | WPRIM | ID: wpr-812801


Objective@#To screen lentiviral vectors carrying siRNA which can specifically down-regulate the gene expression of the sphingosine-1-phosphate receptor 3 (S1PR3) in the corpus cavernosum smooth muscle (CCSM) cells of rats with spontaneous hypertension (SHT) and investigate the influence of the vectors on the signaling pathways of ROCK1, ROCK2 and eNOS in the CCSM cells of SHT rats.@*METHODS@#Using the S1PR3 mRNA sequence of the rat as an interfering target, we designed and synthesized three pairs of siRNA sequences (siRNA1, 2 and 3) targeting S1PR3 and one pair of negative control, and then constructed and packaged them into lentiviral vectors. We cultured the CCSM cells of SHT and Wistar-Kyoto (WKY) rats in vitro and randomly divided them into groups A (SHT untransfected control), B (SHT transfected and carrying negative control virus), C (SHT transfected and carrying siRNA1 targeting S1PR3), D (SHT transfected and carrying siRNA2 targeting S1PR3), E (SHT transfected and carrying siRNA3 targeting S1PR3), and F (WKY untransfected control). With the multiplicity of infection (MOI) = 60, we transfected the CCSM cells of the SHT rats with the lentiviral vector and then determined the expression of the green fluorescent protein (GFP) as well as the mRNA and protein expressions of S1PR3, ROCK1, ROCK2 and eNOS in the CCSM cells of the SHT and WKY rats by RT-PCR and Western blot.@*RESULTS@#Gene sequencing proved the successful construction of the lentiviral vector. The transfection efficiency of the CCSM cells of the rats was >80% in groups B, C, D and E. Compared with group A, the mRNA and protein expressions of S1PR3, ROCK1 and ROCK2 exhibited no significant difference in group B but were remarkably decreased in groups C, D, E and F (P0.05) but remarkably lower than those in group F (P0.05) but markedly increased in groups A, B, C and D (P< 0.05), while those of eNOS remarkably decreased in groups A, B, C, D and E (P< 0.05).@*CONCLUSIONS@#The three constructed lentiviral vectors carrying siRNA targeting different loci of the S1PR3 gene could significantly inhibit the expression of S1P3 as well as RhoA/Rho kinase signaling pathways in the CCSM cells of SHT rats, and the vector carrying siRNA3 exhibited the highest inhibitory effect.

Animals , Down-Regulation , Gene Expression , Genetic Vectors , Green Fluorescent Proteins , Metabolism , Lentivirus , Genetics , Male , Myocytes, Smooth Muscle , Metabolism , Nitric Oxide Synthase Type III , Metabolism , Penis , Metabolism , RNA, Messenger , RNA, Small Interfering , Genetics , Metabolism , Random Allocation , Rats , Rats, Inbred WKY , Receptors, Lysosphingolipid , Genetics , Metabolism , Signal Transduction , Sphingosine-1-Phosphate Receptors , Transfection , rho-Associated Kinases , Metabolism
National Journal of Andrology ; (12): 256-261, 2017.
Article in Chinese | WPRIM | ID: wpr-812776


Objective@#To systematically analyze the correlation between psoriasis and erectile dysfunction (ED).@*METHODS@#We searched the Cochrane Library, EMbase, PubMed, OVID, Medline, VIP, WanFang, China National Knowledge Infrastructure (CNKI), and Chinese Biomedical Literature Database (CBM via SinoMed) for the published literature about the relationship between psoriasis and ED up to June 2016. According to inclusion and exclusion criteria, two researchers respectively extracted the relevant data and made a meta-analysis on the correlation of psoriasis with ED and IIEF-5 scores using the Review Manager 5.3 software.@*RESULTS@#A total of 6 studies were included in this analysis. The analysis with the fixed-effects model revealed a significant correlation between psoriasis and ED (OR = 1.92, 95% CI: 1.53-2.40, P <0.01), and that on 3 of the studies with the random-effects model showed that the IIEF-5 scores were significantly lower in psoriasis patients than in non-psoriasis males (MD = -3.11, 95% CI: -4.85--1.37, P <0.01).@*CONCLUSIONS@#There is a certain correlation between psoriasis and ED. Psoriasis patients may have a higher incidence of ED though it is to be further confirmed by more higher-quality studies.

Erectile Dysfunction , Epidemiology , Humans , Male , Psoriasis , Epidemiology
National Journal of Andrology ; (12): 659-662, 2016.
Article in Chinese | WPRIM | ID: wpr-304694


Psoriasis is a chronic inflammatory disease involving several systems. Recent epidemiological studies show that psoriasis is closely related to erectile dysfunction (ED) and may be an independent factor of ED. Psoriasis-induced ED may be associated with vascular endothelial injury, oxidative stress, mental depression, and so on. An insight into the incidence and pathogenesis of psoriasis-related ED will help to improve psoriasis patients' early understanding of ED, prevent its development and progression, and improve the patients' quality of life.

Depression , Endothelium, Vascular , Erectile Dysfunction , Humans , Incidence , Male , Oxidative Stress , Psoriasis , Quality of Life , Risk Factors
National Journal of Andrology ; (12): 99-103, 2016.
Article in Chinese | WPRIM | ID: wpr-304744


Phosphodiesterase-5 inhibitors (PDE5i) have been used as the first-line treatment for erectile dysfunction (ED) in recent years. However, with the increased clinical application of PDE5i, the incidence rate of PDE5i-induced adverse reactions is on the rise, which may involve the cardiovascular, digestive, nervous, respiratory, and reproductive systems. Most of the adverse reactions are mild to moderate, occasionally with serious or rare complications. The probability and severity of the adverse reactions are associated with the dosage and frequency of medication as well as with individual differences. Therefore individualized medication is necessitated and, for the patients with cardiovascular disease, epilepsy, psychosis, or anaphylactic conditions, PDE5i should be cautiously given or avoided. This review provides an overview of PDE5i-induced adverse reactions and countermeasures in the treatment of ED.

Erectile Dysfunction , Drug Therapy , Humans , Male , Phosphodiesterase 5 Inhibitors