ABSTRACT
It is reported that dihydroartemisinin could reduce the expression of phosphorylated adhesion kinase and matrix metalloproteinase-2, inhibit the growth, migration and invasion of ovarian cancer cells, promote the formation of Treg cells through TGF-beta/Smad signaling pathway, and play an immunosuppressive role; dihydroartemisinin could also inhibit the growth of lung cancer cells by inhibiting the expression of vascular endothelial growth factor(VEGF) receptor KDR. However, there are few studies on dihydroartemisinin in hepatocellular carcinoma cells. In order to preliminarily explore the effect of dihydroartemisinin on invasion and metastasis of hepatocellular carcinoma cells, CCK-8 method and crystal violet staining were used to detect the effect of dihydroartemisinin on the growth of hepatocellular carcinoma cell 7402 and highly metastatic hepatocellular carcinoma cell MHCC97 H. The effects of dihydroartemisinin on the invasion and metastasis of hepatocellular carcinoma cell 7402 and highly metastatic hepatocellular carcinoma cell MHCC97 H were studied by using cell wound healing and Transwell. Western blot was used to detect the protein expression of epidermal growth factor receptor(EGFR) and its downstream signaling pathway in cells treated with dihydroartemisinin for 48 hours. The results showed that dihydroartemisinin could inhibit the growth of hepatocellular carcinoma cell 7402 and highly metastatic hepatocellular carcinoma cell MHCC97 H at 25 μmol·L~(-1). As compared with the control group, the number of cell clones was significantly reduced, and the ability of cell migration and invasion was weakened. Western blot results showed that as compared with the control group, dihydroartemisinin group could down-regulate the protein expression of EGFR and its downstream signaling pathways p-AKT, p-ERK, N-cadherin, Snail and Slug, and up-regulate the expression of E-cadherin protein, thus affecting the migration, invasion and metastasis of hepatocellular carcinoma cells 7402 and MHCC97 H.
Subject(s)
Humans , Artemisinins/pharmacology , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Cell Movement , ErbB Receptors/metabolism , Liver Neoplasms/pathology , Neoplasm Invasiveness , Neoplasm Metastasis , Signal TransductionABSTRACT
Two new compounds, (22E)-25-carboxy-8β,14β-epoxy-4α,5α-dihydroxyergosta-2,22-dien-7-one (1) and fusidione (3), along with two known compounds, 5α,8α-epidioxy ergosta-6,22-diene-3β-ol (2) and microperfuranone (4), were isolated from the fermentation products of the marine-sourced fungus Acremonium fusidioides RZ01. The structures of compounds 1 and 3 were elucidated by extensive spectroscopic methods, especially 2D NMR, and their absolute configurations were suggested on the basis of the circular dichroism spectral analysis and the NOESY data. Both new compounds showed inhibitory activity against HL-60 cells with IC values being16.6 and 44.9 μmol·L, respectively.
Subject(s)
Humans , Acremonium , Chemistry , Classification , Metabolism , Antineoplastic Agents , Chemistry , Pharmacology , Cell Proliferation , Fermentation , HL-60 Cells , Magnetic Resonance Spectroscopy , Molecular Structure , Seawater , MicrobiologyABSTRACT
Seven sinapine analogs (6a-6g) were synthesized using cinnamon acid or benzoic acid and their derivatives as starting materials, which obtained from substituted benzaldehyde and malonate. The structures of target compounds were characterized by IR, 1H NMR and elemental analysis. The effects of compounds 6a-6g on the smooth muscle of intestine isolated from rabbit were studied, and the experimental results showed that compounds 6a, 6d and 6g had diastolic action, while 6f had contractile action.
Subject(s)
Animals , Rabbits , Choline , Chemistry , Pharmacology , In Vitro Techniques , Intestines , Physiology , Molecular Structure , Muscle Contraction , Muscle Tonus , Muscle, Smooth , PhysiologyABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of the roots of Stellera chamaejasme.</p><p><b>METHOD</b>The chemical constituents were separated and purified by chromatographic method after solvent extraction and were identified by spectroscopic analysis.</p><p><b>RESULT</b>Two phenolic compounds were obtained and determined as stelleranol (1) and umbelliferone-7-O-glucoside (2).</p><p><b>CONCLUSION</b>Compound 1 was a new compound, and compound 2 was isolated from this plant for the first time.</p>