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1.
Sichuan Mental Health ; (6): 531-536, 2022.
Article in Chinese | WPRIM | ID: wpr-987359

ABSTRACT

ObjectiveTo investigate the status and influencing factors of medication adherence in patients with severe mental disorders in Zhengzhou, so as to provide references for the formulation of prevention and treatment measures for severe mental disorders. MethodsFrom March to June 2021, a stratified multistage cluster sampling method was applied to select 342 patients from the National Information System for Severe Mental Disorders in Zhengzhou. The general demographic data of patients were collected via self-designed questionnaire, and the medication status was investigated, then the influential factors were summarized. The differences in influential factors of medication adherence were compared between the medication adherence group and the medication non-adherence group. Thereafter, Logistic regression analysis was applied to explore the factors influencing medication adherence. ResultsA total of 320 patients were included in the final analysis, altogether 76.56% of patients (n=245) complied with medication. The differences between patients in the medication adherence group and those in the medication non-adherence group were statistically significant in terms of residence, occupation, and outpatient chronic disease reimbursement (χ2=14.015, 7.502, 13.106, P<0.05 or 0.01). In the questionnaire of influential factors on medication adherence, there were statistically significant differences in the scores of lack of insight, stigma and drug-related factors between the two groups (Z=7.588, 2.379, 2.893, P<0.05 or 0.01). Outpatient chronic disease reimbursement was a protective factor for medication adherence (OR=2.727, 95% CI: 1.320~5.634, P<0.01), while rural residence (OR=0.465, 95% CI: 0.221~0.977, P<0.05) and lack of insight (OR=0.398, 95% CI: 0.286~0.553, P<0.01) were risk factors for medication adherence. ConclusionPatients with severe mental disorders in Zhengzhou have a high rate of medication adherence, moreover, the outpatient chronic disease reimbursement, lack of insight and residence may be influencing factors for medication adherence in patients with severe mental disorders.

2.
Article in Chinese | WPRIM | ID: wpr-514865

ABSTRACT

BACKGROUND:CD133 has been used as a marker to separate and study different tumor stem cells by using its protein specific features. OBJECTIVE:To investigate the expression of stem cell surface marker CD133 in hepatocel ular carcinoma and the in vitro proliferation of its positive subsets. METHODS:The human hepatoma MHCC97-H cell lines were cultured and sorted, CD133+and CD133-MHCC97-H cells were obtained, and the expression of CD133 and cell migration and invasion were detected. After 7 days of culture, cell proliferation ability and Notch expression were detected. After 14 days of culture, cell cloning efficiency was detected. CD133+and CD133-MHCC97-H cells were implanted into nude mice subcutaneously, and 4 weeks later, tumor formation in mice was detected. RESULTS AND CONCLUSION:(1) CD133 expression and cell clone formation:The expression of CD133 and cell cloning efficiency of CD133+MHCC97-H cells were significantly higher than those of CD133-MHCC97-H cells (P<0.05). (2) Cell proliferation:After 3-7 days of culture, the absorbance value of CD133+MHCC97-H cells was significantly higher than that of CD133-MHCC97-H cells (P<0.05). (3) Cell migration and invasion:The number of CD133+MHCC97-H cells passing through the cell membrane was significantly more than that of CD133-MHCC97-H cells (P<0.05). (4) The Notch protein expression of CD133+MHCC97-H cells was significantly higher than that of CD133-MHCC97-H cells (P<0.05). (6) Tumor formation test:The tumor size of CD133 -MHCC97-H cells was larger than that of CD133-MHCC97-H cells (P<0.05). To conclude, CD133+hepatocel ular carcinoma cell subsets with strong proliferation ability have some characteristics of cancer stem cells, and strong invasion, metastasis and tumorigenic abilities.

3.
China Modern Doctor ; (36): 63-66, 2015.
Article in Chinese | WPRIM | ID: wpr-1037759

ABSTRACT

Objective To observe the application of improved Parks incision combined with proparacaine surface anes-thesia in the correction surgery of adolescent concomitant strabismus and the postoperative recovery situation. Methods Fifty-five patients who had received concomitant strabismus correction surgery in our hospital were randomly selected and divided into the improved group and the traditional group, of which the improved group (35 patients) was given the improved Parks incision combined with proparacaine surface anesthesia surgical method and the traditional group (20 patients) was given traditional corneal limbus trapezoid incision combined with lidocaine local anesthesia surgical method. The surgical anesthesia tolerance, postoperative cosmetic results and preoperative and postoperative tear film stability of the two groups were observed. The data were analyzed statistically. Results The patients received propara caine surface anesthesia and the patients received lidocaine local anesthesia were not significantly different in the toler-ance(P>0.05). Regarding to cosmetic results, the Parks incision concealed itself better than the traditional corneal limbus trapezoid incision, and showed faster and better recovery and better postoperative orthotopic effects, with statis-tically significant differences. The improved group was better than the traditional group in the fluorescein staining tear film break-up time (FBUT) and schirmer's Ⅰ experiment (S1T) 30 days after surgery, with statistically significant dif-ferences. Conclusion The improved surgery has better anesthetic tolerance effect, less invasive operation, less damage to normal tissue, better postoperative cosmetic results and smaller influence on ocular surface tear film function than the traditional surgery, thereby worthy of promotion.

4.
Clinical Medicine of China ; (12): 601-602, 2009.
Article in Chinese | WPRIM | ID: wpr-394585

ABSTRACT

Objective To observe the effect of stellate ganglion block (SGB) in the treatment of peri-men-opanse syndrome of clinical efficacy. Methods 30 patients diagnosed as perimenepausal syndorme by the gynecolo-gy clinic in our hospital from February 2007 to December 2008 were selected. All patients experienced vaginal cytolo-gy and examination of blood estradiol (E2),follicle-stimulating generation Su (FSH),luteinizing hormone (LH),in line with perimenopausal syndrome and no other chronic diseases, and in the last 3 months the patients had not taken hormone treatment drugs. Anterior SGB once a day,around the turn was adopted,taking 10 times as a course of treat-ment. All patients were treated for two courses. The blood FSH, LH, E2 changes were recorded. Results Blood E<,2> in-creased from (31.29±19.36) pmol/L to (159.47±88.21) pmol/L(t=-24.976, P<0.01). FSH decreased from (54.67±19.24) U/L to (38.15±13.50) U/L (t=13.872, P<0.01), and LH dropped from (36.1± 15.6)U/L to (26.7±8.7)U/L (t=9.188,P<0.01). Conclusion SGB has the disorder and autonomic Endo-crine function to achieve a new balance because it can adjust perimenopausal autonomic nervous imbalance, so it is the effective treatment for elimination of peri-menopause syndrome.

5.
Article in Chinese | WPRIM | ID: wpr-621678

ABSTRACT

Objective To study the toxic effects of 5-amionlevulinic acid-based photodynamic therapy (ALA-PDT) on human peripheral blood mononuclear cells (PBMCs), cord blood mononuclear cells (CBMCs) and peripheral blood stem cells (PBSCs), and furthermore, to understand the possible causes of this response. Methods We used MTT assay to detect the survival rate of PBMCs, CBMCs and PBSCs after treated by ALA-PDT under the optimum experiment conditions with U937 as control;Annexin V-FITC/PI was used to detect the pattern of cell death induced by ALA-PDT. By using flow cytometry, we detected intracellular PpIX fluorescence intensity. Results After ALA-PDT treatment the survival rate of PBMCs had no significant change;however in PBSCs and CBMCs, the survival rate reduced to 70%, and the survival rate of leukemia cell U937 was the lowest, about 30%. After incubation with ALA,except for PBMCs, intraceUuiar PplX fluorescence intensity of the other two kinds of normal haemocytes and U937 increased obviously. These results combined with the flow cytometry suggested that the main pattern of cell death here was apoptosts. Conclusion Under the optimum experiment conditions, ALA-PDT has a slight effect on normal haemocytes but excellent depletions of leukemia cells. Therefore, it can effectively purify autologons bone marrow or stem cell grafts.

6.
Academic Journal of Xi&#39 ; an Jiaotong University;(4): 250-255, 2008.
Article in Chinese | WPRIM | ID: wpr-844811

ABSTRACT

Objective: To study the toxic effects of 5-amionlevulinic acid-based photodynamic therapy (ALA-PDT) on human peripheral blood mononuclear cells (PBMCs), cord blood mononuclear cells (CBMCs) and peripheral blood stem cells (PBSCs), and furthermore, to understand the possible causes of this response. Methods: We used MTT assay to detect the survival rate of PBMCs, CBMCs and PBSCs after treated by ALA-PDT under the optimum experiment conditions with U937 as control; Annexin V-FITC/PI was used to detect the pattern of cell death induced by ALA-PDT. By using flow cytometry, we detected intracellular PpIX fluorescence intensity. Results: After ALA-PDT treatment the survival rate of PBMCS had no significant change; however in PBSCS and CBMCs, the survival rate reduced to 70%, and the survival rate of leukemia cell U937 was the lowest, about 30%. After incubation with ALA, except for PBMCs, intracellular PpIX fluorescence intensity of the other two kinds of normal haemocytes and U937 increased obviously. These results combined with the flow cytometry suggested that the main pattern of cell death here was apoptosis. Conclusion: Under the optimum experiment conditions, ALA-PDT has a slight effect on normal haemocytes but excellent depletions of leukemia cells. Therefore, it can effectively purify autologous bone marrow or stem cell grafts.

7.
Article in Chinese | WPRIM | ID: wpr-621777

ABSTRACT

Objective To optimize experimental parameters for the photosensitization of 5-aminolevulinic acid (ALA) in promyelocytic leukemia cell HL60 and compare them with normal human peripheral blood mononuclear cell (PBMC). Methods ALA incubation time, wavelength applied to irradiate, concentration of ALA incubated, irradiation fluence may modulate the effect of 5-aminolevulinic acid based Photodynamic Therapy (ALA-PDT).The high-pressure mercury lamps of 400W served as light source, the interference filter of 410nm, 432nm, 545nm, 577nm were used to select the specific wavelength. Fluorescence microscope was used to detect the fluorescence intensity and location of protoporphyrin IX (PpIX) endogenously produced by ALA. MTT assay was used to measure the survival of cell. Flow cytometry with ANNEXIN V FITC kit (contains annexin V FITC, binding buffer and PI) was used to detect the mode of cell death. Results ① 1mmol/L ALA incubated 1×105/mL HL60 cell line for 4 hours, the maximum fluorescence of ALA induced PpIX was detected in cytomembrane. ② Irradiated with 410nm for 14.4J/cm2 can result in the minimum survivability of HL60 cell. ③ The main mode of HL60 cell death caused by ALA-PDT is necrosis. Conclusion ALA for 1mmol/L, 4 hours for dark incubation time, 410nm for irradiation wavelength, 14.4J/cm2 for irradiation fluence were the optimal parameters to selectively eliminate promyelocytic leukemia cell HL60 by ALA based PDT. The photosensitization of ALA based PDT caused the necrosis of HL60 cell, so it could be used for inactivation of certain leukemia cells.

8.
J. biomed. eng ; Sheng wu yi xue gong cheng xue za zhi;(6): 525-529, 2005.
Article in Chinese | WPRIM | ID: wpr-354258

ABSTRACT

This experiment was designed to explore the pattern of K562 and HL60 leukemia cells death, the effects on their cell cycle and the cytoplasmic free calcium concentration ([Ca2+]i) induced by 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT). Under the transmission electron microscope (TEM), two kinds of leukemia cells' ultrastructure were observed. Flow cytometry combined with Annexin V-FITC/PI labeling was used to detect the pattern of K562 and HL60 cells' death induced by ALA-PDT. Flow cytometry combined with PI labeling was used to analyze the change in the cell cycle induced by ALA-PDT, and confocal laser scanning microscopy (CLSM) combining with calcium fluorescence probe was used to detect the change in the cytoplasmic free calcium concentration ([Ca2+]i). Immediately after irradiation, many typical apoptotic bodies were seen in the cells treated. Most of the cells treated were necrotic at 24 hours following irradiation. Flow cytometry analysis suggested that the main patterns of the cells' death were apoptosis immediately after irradiation and necrosis post-apoptosis at 24 hours post irradiation. Immediately and 24 hours after irradiation, the proportion of S phase of K562 was 57. 67% +/- 1.13% and 84.77% +/- 6.20% respectively, and the proportion of S phase of HL60 was 74.60% +/- 7.27% and 84.60% 1.74% respectively. Both [Ca+]i of the treated K562 and HL60 were increased obviously. In the best experiment condition, the initial pattern of the K562 and HL60 leukemia cells' death induced by PDT was apoptosis and the main pattern was necrosis post apoptosis. The two kinds of cells were arrested at S phase by ALA-PDT. During the death of the leukemia cells, the increase in intracellular free calcium concentration could be responsible for the ALA photodynamically induced damage to K562 and HL60 cells.


Subject(s)
Humans , Aminolevulinic Acid , Pharmacology , Apoptosis , Calcium , Chemistry , Cell Division , Cytosol , Chemistry , HL-60 Cells , K562 Cells , Photochemotherapy
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