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1.
Arq. gastroenterol ; 57(3): 296-299, July-Sept. 2020. tab, graf
Article in English | LILACS | ID: biblio-1131665

ABSTRACT

ABSTRACT BACKGROUND: Rutin is a flavonol glycoside that can be found in a wide variety of vegetables and has activity, anti-cancer, anti-inflammatory and anti-diabetic properties. OBJECTIVE: This study investigated the effect of rutin oral administration on Wistar rats submitted to hepatic hyperplasia after partial hepatectomy (PH). METHODS: To achieve this, we considered the analysis of hepatic hyperplastic and plasma biochemical activity of Wistar rats, subjected to treatment with rutin 40 mg/kg/day for 10 days in group 1 (G1) or saline in group 2 (G2), followed by partial hepatectomy. RESULTS: The results indicated an increase in the number of mitoses after 24 hours and 48 hours (P=0.0022 and P=0.0152, respectively) of PH in the group that received rutin, as well as an increase in AST serum levels after 24 hours (P=0.0159) and 48 hours (P=0.0158) and alkaline phosphatase after 24 hours (P=0.015) in the same group, in relation to the respective controls. The group that received rutin showed a more evident variation than the control group when comparing the 24 hour and 48 hour results regarding AST, number of mitoses and number of apoptosis (P<0.005). CONCLUSION: It was concluded that rutin intervened in hepatic hyperplasia after 24 hours and 48 hours of PH, favoring hepatic hyperplasia.


RESUMO CONTEXTO: A rutina é um flavonoide que pode ser encontrado em grande variedade de vegetais e apresenta atividades anticâncer, anti-inflamatória e antidiabética. OBJETIVO: O objetivo deste estudo foi investigar o efeito da administração oral de rutina sobre a hiperplasia hepática em ratos Wistar submetidos à hepatectomia parcial. MÉTODOS: Foi realizada a análise da hiperplasia hepática e da bioquímica plasmática dos ratos Wistar tratados com rutina 40 mg/kg por 10 dias no grupo 1 (G1) ou salina no grupo 2 (G2), seguido da hepatectomia parcial. RESULTADOS: Os resultados indicaram aumento do número de mitoses após 24 e 48 horas (P=0,0022 e P=0,0152, respectivamente) da hepatectomia parcial no grupo que recebeu rutina, além de um aumento nos níveis séricos de AST após 24 horas (P=0,0159) e 48 horas (P=0,0158) e de fosfatase alcalina após 24 horas (P=0,015) no mesmo grupo, em relação aos respectivos controles. O grupo que recebeu rutina mostrou variação mais evidente do que o grupo controle quando se comparou os resultados de 24 horas e 48 horas em relação a AST, número de mitoses e número de apoptoses (P<0,005). CONCLUSÃO: Foi possível concluir que a rutina interferiu na hiperplasia hepática após 24 e 48 horas após a hepatectomia parcial, favorecendo a hiperplasia hepática.


Subject(s)
Animals , Rats , Rutin , Hyperplasia , Aspartate Aminotransferases , Rats, Wistar , Alanine Transaminase
2.
Rev. colomb. ciencias quim. farm ; 49(1): 5-16, Jan.-Apr. 2020. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1144335

ABSTRACT

SUMMARY Food supplements are easily acquired and used in various countries. Silymarin has been indicated for diseases of the liver and Chromium picolinate has been indicated for body weight loss and for the improvement of glycemic index. The objective of the present study was to assess the effects of short-term treatment with a combination of silymarin (50 mg/kg) and chromium picolinate (5 µg/kg) on the standard glibenclamide treatment (10 mg/kg) of rats with induced diabetes. DM2 was induced with streptozotocin. Experimental groups of rats: healthy control group, glibenclamide diabetic group, silymarin diabetic group, and silymarin, chromium picolinate and glibenclamide group. After 10 days of oral treatment, we determined body weight, fasting glycemia, glycemia 1 h after gastric gavage with sucrose, and AST and ALT transaminases. Statistical analysis of the data indicated that there was no change in body weight or fasting glycemia, but that glycemia increased after gavage with sucrose in the group submitted to combined therapy. Thus, we concluded that the combination of silymarin and chromium picolinate reduced the efficacy of glibenclamide in the short term, although the two substances had a protective effect on the liver as observed by the reduction of blood transaminase levels.


RESUMO Suplementos alimentares são de fácil aquisição e uso em diversos países. A silimarina tem sido indicada para desordens hepáticas e o picolinato de cromo tem sido utilizado para perda de peso corporal e melhoria do índice glicêmico. O objetivo deste trabalho foi avaliar os efeitos do tratamento utilizando uma combinação de silimarina (50 mg/kg) e picolinato de cromo (5 µg/kg) sobre o tratamento com glibencla-mida (10 mg/kg) em ratos com diabetes induzida com estreptozotocina. Os grupos experimentais foram: grupo controle sadio, grupo diabético glibenclamida, grupo diabético silimarina e grupo diabético silimarina, picolinato de cromo e glibencla-mida. Após 10 dias de tratamento via oral, determinou-se o peso corpóreo, glicemia de jejum, glicemia após uma hora de gavagem gástrica com sacarose e transaminases hepáticas. A análise estatística dos dados indicou que não ocorreu alteração significativa no peso corpóreo e na glicemia de jejum, mas ocorreu aumento significativo dos níveis glicêmicos no grupo diabético silimarina, picolinato de cromo e glibenclamida após a gavagem com sacarose no grupo com a terapia combinada. Portanto, conclui-se que a combinação utilizada reduziu a eicácia da glibenclamida em curto prazo, embora ambas substancias tenham exibido efeito hepatoprotetor, observado pela redução dos níveis plasmáticos de transaminases.

3.
Ciênc. rural (Online) ; 50(3): e20190587, 2020. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1089562

ABSTRACT

ABSTRACT: Vis-NIR-SWIR reflectance spectra of leaf samples, collected in the laboratory, allow the calibration of predictive models to quantify their physicochemical attributes in a practical manner and without producing chemical residues. This technique should enable the development of management strategies for intensification of pasture use. However, spectral analysis performed in the laboratory may be affected by the deterioration of plant material during transport from the field to the lab, so storage methods are necessary. This research aimed to evaluate the effects of different storage methods on the spectral response of Mombasa grass leaves. Three methods were evaluated: (i) artificially refrigerated environment, (ii) humid environment, and (iii) without microenvironment control. These methods were tested in five different storage times: 2 hours, 4 hours, 8 hours, 24 hours and 48 hours. The spectral behavior of the leaves still inserted in the plant was used as a quality reference. Results showed notable changes at the earliest storage time for the treatment without microenvironment control. Both methods with microenvironment control stabilized the occurrence of spectral changes over 48 hours of the samples storage, thus both were suggested for this species.


RESUMO: Espectros de reflectância vis-NIR-SWIR de amostras foliares, coletados em laboratório, permitem a calibração de modelos preditivos para quantificação de seus atributos físico-químicos de maneira prática e sem produção de resíduos químicos. Esta técnica permite o desenvolvimento de estratégias de manejo para a intensificação do uso de pastagens. Contudo, análises espectrais realizadas em laboratório podem ser afetadas pela deterioração do material vegetal durante o transporte do campo ao laboratório, fazendo-se necessário a utilização de métodos de armazenamento. O presente trabalho objetivou avaliar o efeito de diferentes métodos de armazenamento na resposta espectral de folhas de capim Mombaça. Avaliou-se três métodos: (i) ambiente refrigerado artificialmente; (ii) ambiente úmido; e (iii) ao ar livre, sem controle do microambiente; assim como, cinco diferentes tempos de armazenamento: 2 horas, 4 horas, 8 horas, 24 horas e 48 horas. O comportamento espectral das folhas ainda inseridas na planta foi utilizado como referência de qualidade. Os resultados mostraram alterações pronunciadas para o armazenamento ao ar livre já nos primeiros intervalos de tempo. Ambos métodos com controle de microambiente permitiram estabilizar a ocorrência de alterações espectrais ao longo das 48h de armazenamento das amostras, sendo ambos sugeridos para esta espécie.

4.
Biosci. j. (Online) ; 35(6): 1688-1694, nov./dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1049092

ABSTRACT

This study aimed to evaluate soil biomass and microbial activity and soybean yield under different limestone and gypsum doses and different cover crops. The experiment was carried out in the experimental area of the Fundação de Apoio a Pesquisa Agropecuária de Chapadão, on a Dystrophic Red Latosol, using cultivar Desafio. The experiment consisted of a randomized blocks design, in a split-plot factorial scheme (3x4x3), with three replications. Plots consisted of three gypsum doses: control (without gypsum), recommended dose (2.3 Mg ha-1), and double dose (4.6 Mg ha-1). Subplots consisted of four limestone doses (2, 4, and 6 Mg ha-1) and the control (without limestone). Each block had three different cover crops: Brachiaria, Millet, and allow. The values obtained with the test revealed that brachiaria had better basal respiration in the absence of gypsum. Conversely, millet had better basal respiration in with the gypsum dose. Basal respiration, using brachiaria as cover crop, was higher at the dose of 2700 kg ha-1 of limestone. However, for the fallow and the millet, basal respiration was higher when using the highest limestone dose of 6000 kg ha-1. The variable microbial biomass showed differences between cover crops only in the absence of gypsum. Brachiaria and fallow presented the highest mean for microbial biomass. The use of millet as a cover crop together with gypsum doses increased the microbial biomass. The variables mass of 100 grains and grain yield had higher mean at the limestone dose of 6000 kg ha-1 .


O objetivo do trabalho foi avaliar a biomassa e atividade microbiana do solo, e produtividade da soja sob efeito das diferentes doses de calcário e gesso e diferentes coberturas de solo. O experimento foi conduzido na área experimental da Fundação de Apoio a Pesquisa Agropecuária de Chapadão, sob Latossolo Vermelho Distrófico, com a cultivar Desafio. O delineamento experimental utilizado foi em blocos casualizados, em esquema fatorial (3x4x3) de parcelas subdividas, com 3 repetições. As parcelas foram constituídas por 3 quantidades de gesso agrícola, sendo: controle (sem gesso), recomendado (2,3 Mg ha-1) e o dobro (4,6 Mg ha-1), as subparcelas por 4 doses de calcário (2, 4 e 6 Mg ha-1), além do controle (sem calcário), onde cada bloco apresentou 3 coberturas diferentes: Braquiaria, Milheto e Pousio. Os resultados foram submetidos ao teste de Tukey 5%. Com os valores obtidos pudemos observar que a respiração basal foi melhor para braquiária na ausência de gesso, e na presença o milheto se saiu melhor. A respiração basal, utilizando a braquiária como cobertura, foi maior na dose de 2700 kg ha-1 de calcário. Já utilizando o pousio e o milheto foi na maior dose 6000 kg ha-1. A variável biomassa microbiana apresentou diferença entre as coberturas apenas na ausência de gesso, onde a braquiária e o pousio apresentaram as maiores médias para esta variável. A utilização do milheto como cobertura em conjunto com as doses de gesso acarretou aumento da biomassa microbiana. A massa de 100 grãos e a produtividade de grãos apresentaram maior média na dose de 6000 kg ha-1 de calcário.


Subject(s)
Soybeans , Biomass , Calcium Sulfate , Millets
5.
Rev. ciênc. méd., (Campinas) ; 28(1): 1-10, jan.-mar. 2019. tab
Article in English | LILACS | ID: biblio-1047794

ABSTRACT

Objective This study aimed to extract the essential oil of Curcuma longa rhizomes collected in Brazil, determine its composition by gas chromatography and mass spectrometry, and evaluate its hemolytic action and antimicrobial activity. Methods The oil extraction was performed by hydrodistillation; its composition was determined by GC-MS; the Minimum Inhibitory Concentration was evaluated through microdilution, and the hemolytic activity was analyzed in sheep red blood cells. Results The essential oil's major components are zingiberene (11%), sesquiphellandrene (10%), ß-turmerone (10%), and α-curcumene (5%). It proved efficient at inhibiting Staphylococcus aureus with Minimum Inhibitory Concentrations of 38.8µl/mL, Staphylococcus epidermidis (Minimum Inhibitory Concentrations of 50.0µl/mL), Escherichia coli (Minimum Inhibitory Concentrations of 44.4µl/mL), and Pseudomonas aeruginosa (Minimum Inhibitory Concentrations of 27.7µl/mL). Conclusion Despite provoking hemolysis in sheep red blood cells, the essential oil suggests promising results for a variety of purposes due to its antibacterial properties. Supplementary research is necessary to determine in vivo activity and the potential use of the C. longa essential oil as an antimicrobial agent in diverse situations.


Objetivo Este trabalho teve como objetivo determinar a composição e avaliar as atividades antimicrobiana e hemolítica do óleo essencial de rizomas da Curcuma longa L. Métodos A extração do óleo essencial foi realizada por hidrodestilação e sua composição determinada por cromatografia gasosa com espectrometria de massas. A Concentração Mínima Inibitória foi avaliada por microdiluição e a atividade hemolítica foi analisada em hemácias de carneiro. Resultados Os componentes majoritários encontrados foram zingibereno (11%), sesquipelenadieno (10%), ß-turmerona (10%) e α-curcumeno (5%). O óleo essencial provocou hemólise e inibiu Staphylococcus aureus (Concentração Mínima Inibitória=38,8µl/mL), Staphylococcus epidermidis (Concentração Mínima Inibitória=50,0µl/mL), Escherichia coli (Concentração Mínima Inibitória=44,4µl µl/mL) e Pseudomonas aeruginosa (Concentração Mínima Inibitória=27,7µl/mL). Conclusão Apesar de provocar hemólise, o óleo essencial analisado tem potencial para diversas finalidades, devido às suas propriedades antibacterianas. Estudos complementares são necessários para determinar a atividade in vivo e o potencial uso de óleo essencial de C. longa como agente antimicrobiano em diversas situações.


Subject(s)
Oils, Volatile , Curcuma , Gas Chromatography-Mass Spectrometry , Anti-Infective Agents , Hemolysis
6.
Acta cir. bras ; 34(6): e201900607, 2019. tab, graf
Article in English | LILACS | ID: biblio-1019264

ABSTRACT

Abstract Purpose Coleus forskohlii Briq., a medicinal plant originally from India, has been indicated against heart disease, expiratory disorders, convulsions, and hepatic changes, among others. In view of the broad pharmacological potential of the plant and the scarce information about its effects, the objective of the present study was to investigate the effect of its use for pretreatment of partially hepatectomized rats. Methods The animals were divided into two experimental groups: Control (CG) receiving physiological saline for 10 days before partial hepatetctomy, and Treated (TG) receiving 40 mg Coleus forskohlii/kg/day for 10 days before partial hepatectomy. The treatments were performed by gastric gavage. After the surgical procedure, treatment was continued according to the following groups: CG 24 h, CG 48 h, TG 24 h, and TG 48 hs, and liver tissue and intracardiac blood samples were obtained for histological and biochemical analysis, respectively. Results No significant differences were observed in mitotic or apoptotic index or in the concentrations of the enzymes AST, ALT and alkaline phosphatase, and no areas of fibrosis were detected. Conclusion Treatment with Coleus forskohlii did not interfere with the course of hepatic hyperplasia.


Subject(s)
Animals , Male , Rats , Plant Extracts/administration & dosage , Plectranthus/chemistry , Hepatectomy/methods , Liver/pathology , Aspartate Aminotransferases/blood , Biomarkers/blood , Hepatocytes/drug effects , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Hyperplasia/drug therapy , Liver/surgery , Liver/drug effects
7.
Acta cir. bras ; 33(5): 439-445, May 2018. graf
Article in English | LILACS | ID: biblio-949344

ABSTRACT

Abstract Purpose: To investigate the effects of capsiate treatment on hepatic hyperplasia in partially hepatectomized rats. Methods: The animals were divided into a Capsiate group (CPH), a Capsiate Post-Partial Hepatectomy group (CPPH) and a Partial Hepatectomy Control group (PH). CPH and CPPH animals received 60 mg/kg/day Capsiate for 30 days. Next, the rats underwent partial hepatectomy. CPPH animals continued to receive treatment for 48 h after partial hepatectomy. Liver tissue and intracardiac blood samples were obtained 24 or 48 h after PH. Results: Capsiate treatment interfered with hepatic parameters, reducing the number of mitoses and apoptosis and increasing blood ALT and alkaline phosphatase concentrations. Conclusion: Capsiate treatment preceding hepatic surgery may compromise the initial period of postoperative recovery.


Subject(s)
Animals , Male , Rats , Capsaicin/analogs & derivatives , Hepatectomy , Liver/enzymology , Aspartate Aminotransferases/metabolism , Capsaicin/pharmacology , Rats, Wistar , Apoptosis/drug effects , Alanine Transaminase/metabolism , Alkaline Phosphatase/metabolism , Liver/drug effects , Liver/pathology , Liver Regeneration/drug effects , Mitosis/drug effects
8.
Acta cir. bras ; 32(3): 194-202, Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-837694

ABSTRACT

Abstract Purpose: To investigate the effects of lycopene and resveratrol pretreatment on hepatic hyperplasia in partially hepatectomized rats. Methods: The lycopene group and the resveratrol group received 40 mg/kg/day of lycopene or resveratrol, respectively (dissolved in olive oil or in saline solution, respectively) and administered via a gastric tube for 30 days. The partially hepatectomzed (PH) control groups received saline or olive oil via a gastric tube for 30 days, respectively, and the normal control group received no treatment. Liver tissue and intracardiac blood samples were obtained 24, 36 or 48 h after PH. Results: No areas of fibrosis were detected. No significant changes in mitotic index, in the number of apoptosis events or in aspartate aminotransferase and alanine aminotransferase levels were observed. Conclusions: Lycopene and resveratrol pretreatment did not interfere on hepatic hyperplasia in partially hepatectomized rats.


Subject(s)
Animals , Male , Stilbenes/pharmacology , Carotenoids/pharmacology , Hepatectomy/methods , Liver/surgery , Liver/drug effects , Antioxidants/pharmacology , Aspartate Aminotransferases/blood , Time Factors , Reproducibility of Results , Treatment Outcome , Rats, Wistar , Apoptosis/drug effects , Oxidative Stress/drug effects , Alanine Transaminase/blood , Liver/enzymology , Liver/pathology , Liver Regeneration/drug effects , Mitotic Index
9.
Braz. arch. biol. technol ; 60: e16160138, 2017. graf
Article in English | LILACS-Express | LILACS | ID: biblio-839073

ABSTRACT

ABSTRACT Quercetin has potent antioxidant action and a hepatoprotective role. The aim of this study was to evaluate the hepatoprotective action of quercetin pretreatment in paracetamol-induced liver damage (PILD) and structural injury resulting from partial hepatectomy (PH). In the first model, Wistar rats received oral quercetin (50mg/kg/day) during 8 days. On the 8th day, 3g/kg paracetamol were added. In the second model, the same quercetin dose was given during 7 days and rats were submitted to PH on the 8th day. Blood samples were obtained for determination of enzyme levels. Liver, heart, kidney and lung tissue were also collected for assessment of quercetin biodistribution and/or histological analyses. The results obtained after PILD were more pronounced at 24 hours, as reflected by the reduction of serum ALT levels and by the lower concentration of quercetin in liver at this time point. Quercetin also had a protective effect in groups submitted to PH, as shown by decreased ALT levels after 18 hours, and of AST levels after 18 and 36h. The reduction in serum AST and ALT levels suggest that treatment with quercetin is useful as a preoperative pharmacologic measure and for prevention of liver damage caused by drugs.

10.
Braz. j. pharm. sci ; 50(4): 885-893, Oct-Dec/2014. tab, graf
Article in English | LILACS | ID: lil-741363

ABSTRACT

Curcuma longa is a ginger family aromatic herb (Zingiberaceae) whose rhizomes contain curcuminoid pigments, including curcumin, a compound known for its anti-inflammatory effects. The objective of this study was to obtain curcuminoid-rich extracts, develop topical formulations thereof, and assess the stability and skin permeation of these formulations. Curcuma longa extracts were obtained and used to develop formulations. Skin permeation studies were conducted in a modified Franz diffusion cell system, and skin retention of curcuminoid pigments was quantified in pig ear membrane. Prepared urea-containing gel-cream formulations were unstable, whereas all others had satisfactory stability and pseudoplastic rheological behavior. The amount of curcuminoid pigments recovered from the receptor solution was negligible. The skin concentration of curcuminoid pigments retained was positive (>20 µg/g of skin, mostly in the stratum corneum), considering the low skin permeability of curcumin. We conclude that development of topical formulations containing curcumin or Curcuma longa extract is feasible, as long as adjuvants are added to improve preservation and durability. The formulations developed in this study enabled penetration of curcumin limited to the superficial layers of the skin and then possibly without a risk of systemic action, thus permitting local use as a topical anti-inflammatory.


Curcuma longa é uma erva aromática, pertencente à família Zingiberaceae e seus rizomas contêm pigmentos curcuminoides, dentre eles a curcumina, conhecida por seu efeito anti-inflamatório. O objetivo deste trabalho foi obter extratos ricos nestes pigmentos, desenvolver e avaliar a estabilidade de formulações tópicas e realizar o estudo da penetração cutânea. Extratos de Curcuma longa foram obtidos e utilizados no desenvolvimento de formulações. A penetração e retenção cutâneas de pigmentos curcuminoides foi avaliada em pele de orelha de porco, utilizando célula de difusão de Franz modificada. As formulações de gel creme preparadas que continham ureia foram instáveis e as demais apresentaram estabilidade satisfatória e comportamento reológico pseudoplástico. A quantidade de pigmentos curcuminoides encontrada na solução receptora foi insignificante, enquanto que a quantidade retida na pele foi superior a 20 µg/g de pele. Concluiu-se como possível o desenvolvimento de formulações tópicas contendo curcumina ou extrato de Curcuma longa, conciliando a adição de adjuvantes para a preservação e maior durabilidade da mesma. As formulações promoveram a penetração da curcumina limitada às camadas superiores da pele, possivelmente sem o risco de ocorrência de ação sistêmica sendo possível a sua utilização para ação local com atividade anti-inflamatória.


Subject(s)
Animals , Chemistry, Pharmaceutical , Curcuma/classification , Pigments, Biological , Additives in Cosmetics , Anti-Inflammatory Agents/analysis , Biological Products/classification
11.
Rev. ciênc. méd., (Campinas) ; 17(1)jan.-fev. 2008. ilus
Article in Portuguese | LILACS | ID: lil-509367

ABSTRACT

Objetivo Avaliar comparativamente, por meio de morfometria, a resposta tecidual óssea em ratos a dois tipos de Agregado de Trióxido Mineral: cinza e branco. Métodos Utilizaram-se 16 ratos, machos, adultos, da variedade Wistar, para implantes de Agregado de Trióxido Mineral em falhas ósseas nas tíbias. Na pata esquerda implantouse o Agregado de Trióxido Mineral cinza e, na direita, o branco; outros 16 animais foram utilizados como controle. Ao final de duas, quatro, oito e 16 semanas pósimplante foram retiradas amostras de tecido para análises morfológica e morfométrica. As lâminas histológicas coradas em Hematoxilina e Eosina foram analisadas histomorfometricamente pelo software TPS Dig 1.38. Os resultados foram tratados estatisticamente por ANOVA seguido do teste de Tukey (p<0,05). Resultados O Agregado de Trióxido Mineral foi evidenciado somente na região do periósteo e tecido conjuntivo frouxo adjacente. Não se observou Agregado de Trióxido Mineral na matriz óssea. Na região da fratura, observou-se proliferação osteoblástica e formação de osso primário nos mesmos padrões e proporções daqueles observados nos animais do grupo controle. Na região do periósteo, não se evidenciou nenhum tipo de infiltrado celular que pudesse indicar o processo inflamatório. Não se observou deposição anormal de fibras colágenas ou vasos neoformados em quantidades significativas. Morfometricamente não houve diferenças significativas entre os dois tipos de Agregado de Trióxido Mineral. Conclusão Os dois tipos de Agregado de Trióxido Mineral não interferiram significativamente no processo de regeneração da falha óssea, mostrando-se, nas condições experimentais utilizadas, compatíveis com os tecidos ósseos


Objective The objective of this study is to use morphometry to compare rat bone tissue response to two types of mineral trioxide aggregate: gray and white. Methods Mineral trioxide aggregate was implanted in tibial bone defects of 16 adult, male Wistar rats. Gray mineral trioxide aggregate was implanted in the left hind leg and white in the right hind leg. Another 16 animals were used as controls. Tissue samples were collected for morphologic and morphometric analyses 2, 4, 6, 8 and 16 weeks after the implant. The histological slides were stained with hematoxylin and eosin and analyzed with the software TPS Dig 1.38. The results were analyzed statistically using ANOVA followed by the Tukey test (p<0.05). Results The mineral trioxide aggregate was evidenced only in the periosteal region and adjacent soft connective tissue. Mineral trioxide aggregate was not found in the bone matrix. Osteoblast proliferation and formation of primary bone in the fracture region occurred in the same patterns and proportions as those of the control animals. No type of cell infiltrate was observed in the periosteal region that could indicate an inflammatory process. Abnormal deposition of collagen fibers or significant amounts of newly formed vessels was also not observed. Morphometrically, there were no significant ifferences between the two types of mineral trioxide aggregate. Conclusion The two types of mineral trioxide aggregate did not interfere significantly with the bone regeneration process, thus they were compatible with the bone tissues in the experimental conditions used


Subject(s)
Humans , Analysis of Variance , Biocompatible Materials , Bone and Bones , Bone Regeneration , Eosine Yellowish-(YS) , Hematoxylin , Prostheses and Implants , Rats, Wistar
12.
Rev. ciênc. méd., (Campinas) ; 15(2): 123-130, mar.-abr. 2006. ilus
Article in Spanish | LILACS | ID: lil-467815

ABSTRACT

Blendas biodegradáveis e bioabsorvíveis de policaprolactona e NAFION foram utilizadas como materiais de implantes. A policaprolactona possui alta cristalinidade, o que garente um processo de degradação relativamente lento quando implantada em sistemas biológicos. A utilização do NAFION teve por finalidade a avaliação da influência de um polímero condutor iônico sobre a taxa de deposição de células que constituem o tecido ósseo e, portanto, sua restauração. Foram realizados testes in vivo com blendas, em ratos, contendo 90 e 70.


Subject(s)
Absorbable Implants , Biocompatible Materials , Rats
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