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OBJECTIVE: To optimize the extraction technology of total vitamin E in Euryale ferox. METHODS: With the extraction amount of total vitamin E as reference index, using extraction time, extraction times, ultrasound power and comminution degree as reference factors, single fator test and Box-Behnken design-response surface methodology was used to optimize the extraction technology of total vitamin E from E. ferox. The validation tests were conducted for 3 times (the amounts of E. ferox were 2.0, 20.0, 40.0 g). RESULTS: The optimal extraction technology of vitamin E included that extraction time of 80 min, extraction times of 3 times, ultrasound power of 240 W, comminution degree of 80 mesh. In validation test, extraction rates of total vitamin E were 2.063, 2.103, 2.085 mg/g (RSD=2.6%, 1.5%, 1.3%, n=3), the relative errors of which to predicted value (2.092 mg/g) were 0.14%, 0.53% and 0.33%, respectively. CONCLUSIONS: The optimal extraction technology is reasonable, stable and feasible, and can be used for the extraction of total vitamin E in E. ferox.
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OBJECTIVE To analyze the results of the inhalational allergen detection in children with allergic rhinitis in Tianjin, and to understand the common allergens and their distribution.METHODS Mediwiss (Screen Allergy) was used to determine the specificity of allergen specific IgE in serum. According to sex, age, season, the distribution of allergens was described.RESULTS The most common inhalant allergens in Tianjin were mixed fungus(52.35%), followed by Dermatophagoides pteronyssinus(35.88%), dog dander(24.31%), cat dander(15.10%). The positive single allergen(59.03%) was most common, followed by positive double allergens(25.88%); The positive rate of the boy with inhalation allergen was higher than that of the girl; The positive rate of the inhalation allergen in the infant stage was significantly lower than that in the preschool, the school age, and the adolescent; In summer, the inhaled allergens positive rate was significantly higher than that in winter and spring, above differences were statistically significant(P<0.05). CONCLUSION Mixed fungus, Dermatophagoides pteronyssinus, dog and cat dander are the most common inhalant allergens in children with AR in Tianjin area. The positive rate of allergens has certain regularity in sex, age and season.
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OBJECTIVE@#To analyze the distribution and drug resistance of the pathogenic bacteria in respiratory secretion in children with trachea bronchial foreign bodies so as to assist physicians in clinical prescription.@*METHOD@#Sputum specimens of 622 children with trachea bronchial foreign bodies were collected,and the drug susceptibility test was peformed.@*RESULT@#Pathogenic bacteria were detected in 124(19. 94%) of 622 sputum specimens. Most detected gram-negtive bacilli were highly sensitive to amikacin, ciprofloxacin, ceftriaxone, cefepime and ceftazidime, no strains were resistant to imipenem and meropenem; 42 strains were gram-positive bacilli. The former were highly sensitive to levofloxacin and chloramphenico,the latter were highly sensitive to ciprofloxacin, moxifloxacin and linezolid, no strains were resistant to rifampicin and vancomycin.@*CONCLUSION@#The frequent pathogenic bacteria in respiratory secretion in children with trachea bronchial foreign bodies include gram-negtive bacilli such as enterobacter cloacae, klebsiella pneumonia, escherichia coli, acinetobacter baumannii, serratia marcescens, and gram-positive bacilli such as streptococcus pneumonia,staphylococcus aureus. The detected gram-negtive bacilli were sensitive to imipenem and meropenem;the detected gram-positive bacilli were sensitive to rifampicin and vancomycin.
Subject(s)
Child , Humans , Bacteria , Disease Susceptibility , Drug Resistance, Bacterial , Foreign Bodies , Microbiology , Microbial Sensitivity Tests , Sputum , Microbiology , Trachea , Microbiology , PathologyABSTRACT
OBJECTIVE@#Analysis of multislice CT (MSCT) on the misdiagnosis reasons of children bronchial foreign body, avoid missed diagnosis, to achieve reasonable application.@*METHOD@#Fourteen cases of misdiagnosed cases of data were retrospectively analyzed in our department of suspicious in children with bronchial foreign body, and discuss the misdiagnosis reasons.@*RESULT@#Fourteen cases of misdiagnosis of children with 9 cases by MSCT examination showed no obvious foreign matter. Through bronchoscopy intraoperative found foreign body, 5 cases by multislice CT (MSCT) to found foreign body, 4 cases of phlegm scabs, 1 case is inflammatory granulation, all recovered after treatment. Reasons of misdiagnosis were threshold selection error, scanning level from the inception glottis, imaging error, etc.@*CONCLUSION@#MSCT is a very valuable diagnostic on airway foreign body check method, but there are certain limitations, we should improve the understanding of misdiagnosis and reduce the occurrence of this phenomenon.
Subject(s)
Child, Preschool , Female , Humans , Infant , Male , Bronchi , Diagnostic Errors , Foreign Bodies , Diagnostic Imaging , Retrospective Studies , Tomography, Spiral Computed , MethodsABSTRACT
<p><b>OBJECTIVE</b>To study the abdominal adipose area, serum adiponectin and leptin levels of nonalcoholic fatty liver disease in elderly males.</p><p><b>METHODS</b>A total of 238 elderly males (more than 60 years) were enrolled and divided into three groups: Nonalcoholic fatty liver disease (NAFLD) group (n = 76), matching group (age and body mass index matching with NAFLD group, n = 77), normal control group (n = 85). Serum levels of adiponectin and leptin were measured by RIA (radiological immunological assay). Abdominal adipose area was detected by computer tomography.</p><p><b>RESULT</b>(1) body mass index (BMI), abdominal subcutaneous adipose area, visceral adipose area, total adipose area of NAFLD group and matching group were (26.87+/-2.62) kg/m2 and (26.63+/-1.97) kg/m2, (166.59+/-54.27) cm2 and (147.89+/-50.14) cm2, (148.94+/-53.72) cm2 and (150.06+/-45.47) cm2, (315.25+/-89.42) cm2 and (297.93+/-75.12) cm2, respectively; and were higher than those in control group (P less than 0.01). The abdominal subcutaneous adipose area is higher in NAFLD group than in matching group, however, the abdominal visceral adipose area and total adipose area were not significantly different between those two groups. (2) The serum leptin level in NAFLD group and matching group was significantly higher than that in control group, and serum leptin level was not significantly different between NAFLD group and matching group. The serum adiponectin of NAFLD group [(6.31+/-3.31)mug/ml] was significantly lower than that of matching group [(9.87+/-7.071)mug/ml, P less than 0.01] and control group (P less than 0.01). There was no difference in adiponectin level between matching group and control group. 3) AST, TG, abdominal subcutaneous adipose area, abdominal visceral adipose area were risk factors of NAFLD, while serum adiponectin was protective factor of NAFLD.</p><p><b>CONCLUSION</b>These data indicate that elderly male NAFLD patients manifest abdominal obesity, high serum leptin, low serum adiponecin, and suggest that adiponectin may play a crucial role in the pathogenesis of NAFLD in elderly males.</p>
Subject(s)
Aged , Humans , Male , Adiponectin , Blood , Body Mass Index , Fatty Liver , Leptin , Non-alcoholic Fatty Liver Disease , Obesity , BloodABSTRACT
Objective:To investigate the effect of short-term intensive treatment with insulin on metabolic control,?-cell function and insulin resistance in newly diagnosed type 2 diabetic patients. Methods:Thirty-eight newly diagnosed type 2 diabetic patients were randomly divided into two groups and treated with continuous subcutaneous insulin infusion(CSII) or multiple daily insulin injection(MDI) for 1 month.Fasting blood glucose(FBG) and lipid profiles were measured,and oral glucose tolerance test(OGTT)was performed before and after the treatment.The second phase insulin and C peptide secretions were measured by calculating area under curve(AUC) in OGTT.HOMA model was used to evaluate ?-cell function((HOMA-?)) and insulin resistance. Results:Excellent control of FBG was achieved after 1 month intensive treatment.(HOMA-?) was improved significantly. Conclusion:The excellent metabolic control and ?-cell function improvement can be achieved by short-term intensive insulin therapies like CSII or MDI.