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1.
Journal of Clinical Hepatology ; (12): 135-141, 2021.
Article in Chinese | WPRIM | ID: wpr-862558

ABSTRACT

ObjectiveTo investigate new biomarkers for hepatic alveolar echinococcosis by screening out differentially expressed microRNAs (miRNAs) in the tissues and plasma of patients with hepatic alveolar echinococcosis, since hepatic alveolar echinococcosis is caused by the infection of multilocular hydatid cyst. MethodsPatients with hepatic alveolar echinococcosis diagnosed in Qinghai University Affilrated Hospital from June 2016 to May 2018 were in cluded. Two marginal tissue samples and three adjacent normal tissue samples were collected from patients with hepatic alveolar echinococcosis, and plasma samples were collected from three patients with hepatic alveolar echinococcosis and three healthy controls. Agilent Human miRNA microarray was used to obtain the miRNA expression profile in tissue and plasma, and differentially expressed miRNAs were screened out based on fold change (FC>1.2) and P value (P<0.05). Plasma miRNAs and tissue miRNAs associated with liver diseases were selected based on target gene prediction of differentially expressed miRNAs and literature reports, and quantitative real-time PCR (qRT-PCR) was used for validation. The t-test was used for comparison of continuous data between two groups. A spearman analysis was used to investigate correlcction. ResultsThere was a significant difference in microRNA expression profile between the patients with hepatic alveolar echinococcosis and the health individuals, and qRT-PCR found that three miRNAs (hsa-miR-4644, hsa-miR-136-5p, hsa-miR-483-3p) were significantly differentially expressed in patients with hepatic alveolar echinococcosis (P<0.05), among which hsa-miR-4644 and hsa-miR-483-3p were significantly upregulated (P<0.05) and hsa-miR-136-5p was significantly downregulated (P<0.05) in patients with hepatic alveolar echinococcosis. Target gene prediction was performed for miRNAs based on TargetScan, PITA, and microRNAorg databases, and the intersection of the target genes predicted by these three databases showed that 137 genes were targeted with miRNAs. The differentially expressed miRNA hsa-miR-483-3p was involved in the target regulation of the genes (IL17A, IL5, CD40LG, TAP2, and TNF) associated with immune response and liver diseases. Gene ontology and Kyoto Encyclopedia of Genes and Genome analyses showed that the target genes of hsa-miR-483-3p played an important role in the primary immunodeficiency signaling pathway, the IL-17 signaling pathway, and the TNF signaling pathway. ConclusionHepatic alveolar echinococcosis has a unique microRNA expression profile, among which hsa-miR-483-3p can be used as a new biomarker for hepatic alveolar echinococcosis, and the target genes regulated by this miRNA are mainly involved in the primary immunodeficiency signaling pathway, the IL-17 signaling pathway, and the TNF signaling pathway. However, further studies are needed to verify the regulatory relationship between these miRNAs and hepatic alveolar echinococcosis.

2.
Article in Chinese | WPRIM | ID: wpr-880464

ABSTRACT

Based on the existing information construction foundation of the isolation ward of the hospital, according to the relevant guidelines issued by the National Health Commission, the management of environmental isolation, disinfection, medical staff management and patient management are discussed, combining the application of Internet of things technology in hospital management, a series of new applications with distinctive features of Internet of Things (IoT) are built, and advanced technology and equipment such as Internet of Things are introduced. Realize the application scenario, implementation method and business mode of intelligent IoT in isolation ward, form an integrated data management center and monitoring system through data intelligent IoT, aggregation and operation, and realize the digital collection, processing, storage, transmission and analysis of medical information, equipment information, personnel information and management information, so as to realize medical closed-loop management, reduce the hidden danger of medical safety in isolated wards and improve the level of medical quality.


Subject(s)
Hospitals , Humans , Internet , Internet of Things , Monitoring, Physiologic , Technology
3.
Journal of Clinical Hepatology ; (12): 2914-2918, 2021.
Article in Chinese | WPRIM | ID: wpr-906888

ABSTRACT

Liver biopsy is the gold standard for the diagnosis of liver fibrosis stage in patients with chronic hepatitis B, but it has certain limitations due to its invasiveness. At present, elastography and serological examination have the advantages of convenience, good repeatability, and dynamic testing and are thus widely used in clinical practice. The accuracy of testing has been supported by a large number of studies, and they have been gradually recommended by various guidelines and expert consensus. However, the diagnostic accuracy of the two methods is affected by factors such as inflammation, obesity, ascites, eating, and intercostal space stenosis, and elastography combined with serological examination can greatly improve the accuracy, sensitivity, and specificity of liver fibrosis staging. With reference to related articles in China and globally, this article reviews the problems of the two methods in the staging of chronic hepatitis B liver fibrosis.

4.
Journal of Clinical Hepatology ; (12): 2737-2741, 2021.
Article in Chinese | WPRIM | ID: wpr-905033

ABSTRACT

Benign biliary stricture (BBS) refers to complete or incomplete stricture of the biliary tract caused by a series of non-malignant diseases. BBS often has complex and diverse etiologies, and severe complications may occur if it is not adequately treated. Diagnostic methods currently used in clinical practice include imaging, endoscopic retrograde cholangiopancreatography, endoscopic ultrasonography, and choledochoscopy, and treatment methods include balloon dilatation, stent implantation, percutaneous transhepatic biliary drainage, and surgical treatment. At present, endoscopic diagnosis and treatment of BBS has become the preferred method. However, there is still no clear classification of BBS, which needs further investigation. By consulting related literature in China and globally, this article summarizes the issues associated with the endoscopic diagnosis and treatment of BBS.

5.
Chinese Journal of Digestion ; (12): 241-246, 2021.
Article in Chinese | WPRIM | ID: wpr-885747

ABSTRACT

Objective:To investigate the efficacy of double balloon enteroscopy (DBE) in the treatment of bleeding from small intestinal vascular lesion and risk factors of bleeding recurrence .Methods:From April 2013 to May 2020, at Air Force Medical Center, the clinical data of 65 patients with confirmed or suspected bleeding from small intestinal vascular lesion were retrospectively analyzed. The patients were divided into DBE treatment group (patients of Yano classification 1a and 1b received argon plasma coagulation, and patients of Yano classification 2 and 3 accepted combination of titanium clip and submucosal injection of lauromacrogol sclerosing agent) and non-DBE treatment group (traditional treatments such as stopping anticoagulant or antiplatelet drugs, blood transfusion, and iron supplementation). The bleeding recurrence of patients with single small intestinal vascular lesion between DBE treatment group and non-DBE treatment group, and patients with single or mulitiple vascular lesion of DBE treatment group were compared. Univariate analysis was used to analyze the clinical data of patients with or without recurrent bleeding. Multivariate logistic regression model was used to analyze the independent risk factors and protective factors of recurrent bleeding in small intestinal vascular lesion. Independent sample t test, chi-square test and Fisher exact probability method were used for statistical analysis. Results:Forty-four (25 of single vascular lesion and 19 of multiple vascular lesion) patients were diagnosed with small intestinal vascular lesions and received DBE treatment (DBE treatment group). Twenty-one patients with single vascular lesion accepted traditional treatment (non-DBE treatment group). The recurrent rate of bleeding in patients with single vascular lesion of DBE treatment group was lower than that in patients with single vascular lesion of non-DBE treatment group and patients with multiple vascular lesion of DBE treatment group (24.0%, 6/25 vs. 71.4%, 15/21 and 12/19), and the differences were statistically significant ( χ2=10.348 and 6.848, P=0.001 and 0.009). The results of univariate analysis showed that the proportion of blood transfusion, hypertension, complicated with valvular heart disease and DBE treatment in patients with rebleeding or not rebleeding from small intestinal vascular lesion was different with statistically significant (69.7%(23/33) vs. 37.5%(12/32), 51.5%(17/33) vs. 18.8%(6/32), 42.4%(14/33) vs. 12.5%(4/32) and 54.5%(18/33) vs. 81.2%(26/32), χ2=6.777, 7.628, 7.265, and 5.298, all P<0.05). The results of multivariate logistic regression analysis indicated that blood transfusion during the course of disease (odds ratien ( OR)=3.736, 95% confidence interval ( CI) 1.082 to 12.898, P=0.037) and complication with valvular heart disease ( OR=4.916, 95% CI 1.107 to 21.829, P=0.036) were independent risk factors of bleeding recurrence in patients with small intestinal vascular lesions. DBE treatment was the protective factor of bleeding recurrence in patients with small intestinal vascular lesion ( OR=0.214, 95% CI 0.057 to 0.808, P=0.023). Conclusions:DBE is effective in the treatment of small intestinal vascular lesion bleeding, especially for single vascular lesion. Blood transfusion during disease course and complication with valvular heart disease are independent risk factors for bleeding recurrence in patients with small intestinal vascular lesion.

6.
Article in Chinese | WPRIM | ID: wpr-868814

ABSTRACT

Objective:To analyze the risk factors for bile leakage in patients undergoing cystic echinococcosis surgery to establish a nomogram model to predict the risk of bile leakage.Methods:The clinical data were retrospectively analyzed that 203 patients with cystic echinococcosis treated in Qinghai University Affiliated Hospital from January 2015 to October 2018. Logistic regression was used to screen out independent risk factors of biliary leakage in patients undergoing cystic hydatid surgery. Then the R software was used to establish a predictive nomogram model. Bootstrap method was used to validate the nomogram model and ROC curve was used to explore the predictive efficacy of the model in predicting the bile leakage.Results:There were 54 cases of biliary leakage in 203 patients with cystic echinococcosis, the incidence was 26.1%. ALP ( OR=11.193, 95% CI 5.066-24.731), GGT ( OR=2.728, 95% CI 1.246-5.975), cyst diameter ( OR=3.491, 95% CI 1.550-7.861), hilar cyst ( OR=2.503, 95% CI 1.176-5.329) were all independent risk factors for biliary leakage of cystic echinococcosis. The consistency index was 0.835 for predicting PPC risk, and the area was 0.823 (95% CI 0.754-0.892) under the curve for predicting the risk of cystic echinococcal bile leakage. Conclusions:ALP, GGT, cyst diameter and hepatic hilar cyst are independent risk factors for cystic echinococcal bile leakage. Nomogram model can evaluate the risk of cystic echinococcal bile leakage more intuitively, with potentially high clinical application value.

7.
Journal of Clinical Hepatology ; (12): 2627-2631, 2019.
Article in Chinese | WPRIM | ID: wpr-778682

ABSTRACT

Cholecystectomy is the most common cause of iatrogenic bile duct injury. The causes, classification and diagnosis of bile duct injury were analyzed. According to the different opportunity of bile duct injury, different treatment strategies and infection control were adopted. The selection of definitive repair of bile duct injury, especially the key points of Roux-Y cholangiojejunostomy, was described in detail. In addition, under the condition of strict indication, it can be combined with hepatectomy or even liver transplantation.

8.
Article in English | WPRIM | ID: wpr-311317

ABSTRACT

The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin (300 ng/kg chow) for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS. Treated rats showed extensive areas of chondrocyte necrosis and superficial cartilage defect in the articular cartilage. In addition, 8 metabolites were found to change significantly in these rats compared to the control group, including lysoPE (18:0/0:0), lysoPC(14:0), lysoPC[18:4 (6Z,9Z,12Z,15Z)], lysoPC[(16:1(9Z)], lysoPC(16:0), L-valine, hippuric acid, and asparaginyl-glycine. These 8 metabolites associated with cartilage injury are mainly involved in phospholipid and amino acid metabolic pathways.

9.
Article in Chinese | WPRIM | ID: wpr-702031

ABSTRACT

Objective To investigate the application of temporal and frontotemporal puncture or frontotemporal craniotomy for hypertensive intracerebral hemorrhage in basal ganglia region .Methods From January 2013 to April 2017,eighty patients with basal ganglia region hypertensive intracerebral hemorrhage in Shanxi Da Hospital were divided into observation group and control group according to random number table method ,with 40 cases in each group.The two groups were respectively given puncture of temporal and frontal temporal region and craniotomy with frontal and temporal bone flap for evacuation of hematoma and decompression of bone flap .The complications and treatment effect after surgery of the two groups were compared.Results Compared with the control group , the emptying rate of postoperative hematoma 1 day in the observation group was decreased [(60.1 ±9.7)%vs.(81.0 ± 9.9)%,t=-9.537,P<0.05];the operation time was shortened [(68.5 ±7.7) min vs.(110.2 ±9.1) min,t=-22.124,P<0.05];the use of postoperative mannitol decreased [(672.1 ±84.2) g vs.(1427.2 ±99.6) g,t=-36.617,P<0.05];the incidence rate of intracranial pneumatosis in the observation group was increased (7.5%vs. 30.0%,χ2=6.646,P<0.05).Conclusion Monitoring of intracranial pressure in lateral ventricles combined with puncture of temporal and frontal temporal region used in hypertensive intracerebral hemorrhage in basal ganglia region is helpful to reduce the surgical trauma and accelerate the recovery of postoperative patients ,and can be widely used.

10.
Article in Chinese | WPRIM | ID: wpr-690520

ABSTRACT

The type 2 diabetes rat model was induced with high-sugar and high-fat diet combined with low-dose STZ. This study investigated the hypolipidemic mechanism of Coptis chinensis and C. deltoidea. After 30 days of administration, HOMA-IR and the content of TG in serum were detected, and the expressions of SCAP, SREBP-1c were tested by the method of Western blot and Real-time PCR analysis. The test results showed that both components can significantly alleviate insulin resistance and down-regulate the expressions of SREBP-1c and SCAP in liver tissue of type two diabetes mellitus. Compared with the control group, there were significant differences in relevant protein expression (<0.05, <0.01). This indicates that the inhibition of SREBP-1c and SCAP expressions may be the hypolipidemic mechanism of Coptidis Rhizoma on type 2 diabetes mellitus. The results also showed that C. deltoidea has a better efficacy in lipid elimination, but a weaker hypoglycemic effect against C. chinensis.

11.
Article in Chinese | WPRIM | ID: wpr-688041

ABSTRACT

<p><b>OBJECTIVE</b>This study aimed to determine the effects of copper content on the corrosion resistance of CoCrMoCu alloy and its in vitro antibacterial performance.</p><p><b>METHODS</b>CoCrMoCu specimens with different Cu contents (2%, 3%, 5%, and 7%) were prepared by vacuum melting method. CoCrMo without Cu served as control. The corrosion resistance of the specimens was measured by electrochemistry. The antibacterial effects of the specimens on Staphylococcus aureus and Escherichia coli were analyzed by coating-film method.</p><p><b>RESULTS</b>Compared with CoCrMo without Cu, the addition of 2%-5% Cu to CoCrMo improved the pitting and uniform corrosion of CoCrMo alloy and decreased the corrosion current density. The antibacterial performance of CoCrMoCu alloy increased with increased Cu content. The antibacterial rate of alloy was 99% when Cu content exceeded 5%.</p><p><b>CONCLUSIONS</b>Cu addition had a statistically significant influence on the corrosion resistance and antibacterial performance of CoCrMoCu. CoCrMoCu has better corrosion resistance and antibacterial performance when Cu content is 5%.</p>

12.
Chinese Journal of Biotechnology ; (12): 1776-1785, 2017.
Article in Chinese | WPRIM | ID: wpr-243672

ABSTRACT

To gain more insights into the rice base editor (rBE3 and rBE4), we evaluated the mutation efficiency, off-target and inheritance of OsSERK1(D428N) and pi-ta(S918F) genes modified with rBE endonucleases. We predicted and analyzed the putative off-target sites of the sgRNA designed for OsSERK1(D428N) and pi-ta(S918F) by PCR amplification and Sanger sequencing. Then we further characterized the inheritance and stability of targeted base mutations and T-DNA segregation in the progeny of the self-fertilized T0 plants. Analysis of the DNA sequencing data of T0 plants of OsSERK1(D428N) revealed no nucleotide change at any of the four potential off-target sites. For OsSERK1(D428N) and Os08g07774 carry the same sgRNA targeting sites, base substitution at both two loci were detected at a frequency of 41.67%. The targeted base mutations could be transmitted readily to T1 progeny. Furthermore, genetic segregation caused the loss of T-DNA at a frequency between 25.0% and 40.9% in the T1 transgenic plants of OsSERK1(D428N) and pi-ta(S918F). These results demonstrated that the rBE3 and rBE4 systems could mediate specifically targeted base editing in one- or multi-site, and the targeted base editing could be stably inherited to next generation.

13.
Journal of Preventive Medicine ; (12): 989-991,998, 2015.
Article in Chinese | WPRIM | ID: wpr-792451

ABSTRACT

Objective To analyze the dominant genotypes of porin Ⅰ gene encoding major outer membrane protein of Neisseria gonorrhoeae in Zhejiang Province and to construct the prokaryotic expression systems of porinⅠA and porinⅠB genes.Methods Based on the previous research,the porinⅠA and porinⅠB genes were sequenced after T -A cloning, and the dominant genotypes of porinⅠA and porinⅠB genes were analyzed.Then the prokaryotic expression systems of the dominant genotypes of porinⅠA and porinⅠB genes were constructed.Ten percent SDS -PAGE was applied to measure the output of PⅠA and PⅠB proteins after inducement by 0.5 mmol/L IPTG.Results All 5 porinⅠA isolates sequenced were serovar ⅠA -6.Of the 11 porinⅠB isolates sequenced,there were 5 serovarⅠB -3 isolates,3 serovarⅠB -3 /6 isolates,1 serovarⅠ B -6 isolate and 2 mutant isolates.Outputs of P Ⅰ A and P Ⅰ B expressed by the constructed prokaryotic expression systems PET -42 -PⅠA and PET -42 -PⅠB were as high as 30% and 20% respectively. Conclusion ⅠA -6 is the dominant genotypes of porinⅠA gene and ⅠB -3 is the dominant genotypes of porinⅠB gene in Zhejiang Province.Comparing to the porinⅠB gene,porinⅠA gene is more conserved.The prokaryotic expression systems with high efficiency of porinⅠA and porinⅠB genes were successfully constructed,which may be helpful in the further research of genetic engineering vaccine and clinical detection of Neisseria gonorrhoeae.

14.
Article in Chinese | WPRIM | ID: wpr-286404

ABSTRACT

<p><b>OBJECTIVE</b>To explore the correlation between Pi and Shen by observing the relationship between the metabolism of aristolochic acid (AA) and mRNA and protein expression levels of organic anion transporting polypeptide (oatp) superfamily member 2a1 and 2 b1 (oatp2al and oatp2bl) in renal, small intestinal, and large intestinal tissues of Pi deficiency syndrome (PDS) model rats.</p><p><b>METHODS</b>Totally 46 Sprague-Dawley (SD) rats were randomly divided into four groups, i.e., the blank group (n = 12), the PDS group (n = 22), the AA-I group (n = 6), and the PDS AA-I group (n = 6). PDS model was established by subcutaneously injecting Reserpine at the daily dose of 5 mg/kg for 16 successive days. Carotid intubation was performed in 6 rats selected from the blank group and the PDS group. Pharmacokinetics of AA-I were detected at 5, 15, 30, 45, and 60 min after gastrogavage of AA-I. AA-I concentrations in renal, small intestinal, and large intestinal tissues of 10 rats selected from the PDS group were determined. Normal saline was administered to 6 rats selected from the PDS group and the blank group by gastrogavage. Renal, small intestinal, and large intestinal tissues were collected in the AA-I group and the PDS AA-I group at 60 min after gastrogavage of AA-I. mRNA and protein expression levels of oatp2a1 and oatp2b1 in each tissue were detected using real-time polymerase chain reaction (RT- PCR) and Western blot.</p><p><b>RESULTS</b>Compared with the blank group, plasma concentrations of in vivo AA-I were obviously higher in the PDS group at 15, 30, 45, and 60 min after gastrogavage of AA-I with statistical difference (P < 0.05). Plasma concentrations of AA-I were obviously decreased at 60 min after gastrogavage of AA-I; AA-I concentrations in renal and large intestinal tissues were elevated; AA-I concentrations in small intestinal tissues were obviously reduced in the PDS group. There was no statistical difference in mRNA expression levels of oatp2a1 and oatp2b1 in the aforesaid three tissues of rats between the blank group and the PDS group. Compared with the blank group, mRNA expression levels of oatp2a1 and oatp2b1 decreased in small intestinal tissues of the AA-I group, and the mRNA expression level of oatp2a1 in large intestinal tissues significantly decreased (P < 0.05, P < 0.01). Compared with the PDS group, mRNA expression levels of oatp2a1 and oatp2b1 increased in renal tissues of the PDS AA-I group (P < 0.05); mRNA expression levels of oatp2b1 increased in large intestinal tissues of the PDS AA-I group (P < 0.05).</p><p><b>CONCLUSIONS</b>The difference in AA-I metabolism might be associated with changed expression levels of oatp2a1 and oatp2b1 in renal, small intestinal, and large intestinal tissues under Pi deficiency induced loss of transportation. Shen and Dachang played important roles in substance metabolism under Pi deficiency state, which proved Pi-Shen correlated in Chinese medical theories.</p>


Subject(s)
Animals , Anions , Aristolochic Acids , Metabolism , Drugs, Chinese Herbal , Kidney , Medicine, Chinese Traditional , Organic Cation Transport Proteins , Metabolism , Peptides , RNA, Messenger , Rats , Rats, Sprague-Dawley
15.
Journal of Preventive Medicine ; (12): 893-896, 2014.
Article in Chinese | WPRIM | ID: wpr-792336

ABSTRACT

Objective To analyze the influence of different therapeutic environments on stigma of schizophrenia patients. Methods A total of 70 outpatients and 70 inpatients with schizophrenia in remission in Tongde hospital of Zhejiang province were enrolled and investigated by using Brief Psychiatric Rating Scale (BPRS),Positive and Negative Syndrome Scale (PANSS),Treatment Emergent Symptom Scale (TESS)and Clinician's Global Impressions-Severity of Illness Scale (CGI-SI).Results Both outpatients and inpatients had been discriminated and had the situation of stigma and disease concealing.After inpatient treatment,the scores of disease concealing factor and positive effect factor were 2.01 ±0.88 and 1.68 ±0.78 respectively,which were significantly lower than those of outpatients (2.43 ±0.98 and 2.44 ±0.78, respectively,P<0.05 ).Conclusion Outpatient treatment has the influence on reduce stigma and improve the life quality of schizophrenia patients in remission.

16.
Journal of Preventive Medicine ; (12): 557-560,564, 2014.
Article in Chinese | WPRIM | ID: wpr-792306

ABSTRACT

Objective The aim of our study is to establish a proper performance evaluation index system of community disease control and prevention for both urban and rural areas in Hangzhou city. Methods The evaluation indexes were selected by Delphi method with two rounds,and the weights of the indexes were determined by analytical hierarchy process. Results A total of 3 first grade indexes were selected with indexes weight of 0. 18,0. 46 and 0. 36,11 second grade indexes were selected of which 4 indexes including funds guarantee,disease control and prevention,immunization program and work performance had relative high weight of 0. 46,0. 24,0. 29 and 0. 62 respectively. Moreover,50 third grade indexes were determined and the indexes considering traditional disease control and prevention had higher indexes weight. Conclusion The newly index system have reasonable structure and comprehensive contents. It could be used to guide the practical work of community disease control and prevention in Hangzhou city.

17.
Article in Chinese | WPRIM | ID: wpr-313050

ABSTRACT

<p><b>OBJECTIVE</b>To explore different regulatory effects of electroacupuncture (EA) at the same acupoint on the heart and the stomach.</p><p><b>METHODS</b>Eighty male SD rats were randomly divided into eight groups equally, i.e., the Neiguan (PC6) group, the Quchi (LI11) group, the Tianshu (ST25) group, the Danzhong (RN17) group, the Zusanli (ST36) group, the Sanyinjiao (SP6) group, the Xinshu (BL15) group, and the Weishu (BL21) group, 10 in each group. The regulatory effects of EA (2/15 Hz, 3 mA, 2 min) at different acupoints on the gastric motility (intragastric pressure) and the cardiac function (the left ventricular pressure) were observed. The mean values of effect of two-min pre-EA and after-EA were compared.</p><p><b>RESULTS</b>Compared with the pre-EA in the same group, the left ventricular pressure decreased in the Neiguan (PC6) group, the Quchi (LI11) group, the Danzhong (RN17) group, the Sanyinjiao (SP6) group, and the Xinshu (BL15) group (P <0.05). The intragastric pressure increased in the Quchi (LI11) group, the Zusanli (ST36) group, the Sanyinjiao (SP6) group, and the Xinshu (BL15) group, while it decreased in the Tianshu (ST25) group, the Danzhong (RN17) group, and the Weishu (BL21) group (P <0.05). The difference of the left ventricular pressure was sequenced from high to low as Xinshu (BL15) >Danzhong (RN17) >Neiguan (PC6) >Sanyinjiao (SP6) >Quchi (LI11). The difference of the intragastric pressure was sequenced from high to low as Tianshu (ST25) >Weishu (BL21) > Xinshu (BL15) > Danzhong (RN17) > Zusanli (ST36) > Sanyinjiao (SP6) > Quchi (LI11).</p><p><b>CONCLUSIONS</b>EA at the same acupoint (2/15 Hz, 3 mA) showed different regulatory effects on the heart and stomach. There existed some difference in the direction and size due to the acupoint location, meridians, dominant nerve segment or relating organs. A fixed effect direction to the specific target was also shown.</p>


Subject(s)
Acupuncture Points , Animals , Electroacupuncture , Heart , Physiology , Male , Meridians , Rats, Wistar , Stomach , Physiology
18.
Article in Chinese | WPRIM | ID: wpr-324189

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the pollution of hexavalent chromium in the electroplating workplace and screen the biomarkers of chromium exposure.</p><p><b>MATERIAL</b>Field occupational health investigation was conducted in 25 electroplating workplaces. 157 electroplating workers and 93 healthy unexposed controls were recruited. The epidemiological information was collected with face to face interview. Chromium in erythrocytes was determined by graphite furnace atomic absorption spectrophotometer.</p><p><b>RESULTS</b>The median of short-term exposure concentration of chromium in the air at electroplating workplace was 0.06 mg/m(3) (median) and ranging from 0.01 (detect limit) to 0.53 mg/m(3)). The median concentration of Cr (VI) in erythrocytes in electroplating workers was 4.41 (2.50 ∼ 5.29) µg/L, which was significantly higher than that in control subjects [1.54 (0.61 ∼ 2.98) µg/L, P < 0.01]. After stratified by potential confounding factors such as gender, age, smoking status and alcohol consumption, significant differences still existed between electroplating workers and control subjects, except for the subjects of age less than 30 years old (P = 0.11).</p><p><b>CONCLUSION</b>There was hexavalent chromium pollution in electroplating workplace. Occupational hazards prevention measures should be taken to control the chromium pollution hazards.</p>


Subject(s)
Adult , Air Pollutants, Occupational , Chromium , Blood , Electroplating , Environmental Monitoring , Erythrocytes , Chemistry , Female , Humans , Male , Middle Aged , Occupational Exposure , Workplace , Young Adult
19.
Article in Chinese | WPRIM | ID: wpr-246357

ABSTRACT

<p><b>OBJECTIVE</b>To observe the efficacy of acupuncture and moxibustion on female obesity complicated with climacteric syndrome and its impact factors.</p><p><b>METHODS</b>One hundred and fifty-one cases of female obesity complicated with climacteric syndrome were treated according to the basic principle as "pattern/syndrome differentiation". Accordingly, the reinforcing or reducing technique was applied at Quchi (LI 11), Zhongwan (CV 12), Tianshu (ST 25), Daheng (SP 15), Zusanli (ST 36), Shangjuxu (ST 37), Sanyinjiao (SP 6), Neiting (ST 44), etc. The auricular point sticking was applied at Hunger Point, Ear-shenmen, Sympathetic Nerve, Endocrine and Stomach. The treatment was given once every two days. The duration of treatment was 3 months. The changes in the symptoms, physical signs, obesity index, Kupperman index, the vegetative equilibrium index (Value Y), estradiol (E2) and follicle stimulating hormone (FSH) were observed before and after treatment.</p><p><b>RESULTS</b>The obesity patients complicated with climacteric syndrome presented unusually high level of obesity index, Kupperman index and FSH levels and unusually low level of E2. After treatment, the obesity index, Kupperman index and FSH levels were all reduced significantly (all P < 0.01) while E2 level was increased significantly (P < 0.05).</p><p><b>CONCLUSION</b>Acupuncture and moxibustion achieve the significant efficacy on obesity complicated with climacteric syndrome, which is closely related with the pattern/syndrome of the disorder and obesity grade. For the effective and curative rate of obesity index, the efficacy of liver qi stagnation pattern/syndrome is better than that at liver stagnation and spleen deficiency pattern/syndrome than spleen and kidney yang deficiency pattern/syndrome than liver and kidney yin deficiency pattern/syndrome than yin and yang deficiency pattern/syndrome; the efficacy of mild obesity is better than that at moderate obesity than severe obesity.</p>


Subject(s)
Acupuncture Therapy , Adult , Female , Humans , Middle Aged , Moxibustion , Obesity , Metabolism , Therapeutics , Postmenopause , Metabolism
20.
Acta Pharmaceutica Sinica ; (12): 1134-1140, 2012.
Article in Chinese | WPRIM | ID: wpr-274687

ABSTRACT

This study is to establish an artificial neural network (ANN) for predicting blood tacrolimus concentration in liver transplantation recipients. Tacrolimus concentration samples (176 samples) from 37 Chinese liver transplantation recipients were collected. ANN established after network parameters were optimized by using momentum method combined with genetic algorithm. Furthermore, the performance of ANN was compared with that of multiple linear regression (MLR). When using accumulated dose of 4 days before therapeutic drug monitoring (TDM) of tacrolimus concentration as input factor, mean prediction error and mean absolute prediction error of ANN were 0.02 +/- 2.40 ng x mL(-1) and 1.93 +/- 1.37 ng x mL(-1), respectively. The absolute prediction error of 84.6% of testing data sets was less than 3.0 ng x mL(-1). Accuracy and precision of ANN are superior to those of MLR. The correlation, accuracy and precision of ANN are good enough to predict blood tacrolimus concentration.


Subject(s)
Adult , Aged , Drug Monitoring , Methods , Female , Humans , Immunosuppressive Agents , Blood , Linear Models , Liver Transplantation , Male , Middle Aged , Neural Networks, Computer , Tacrolimus , Blood
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