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1.
Article in Chinese | WPRIM | ID: wpr-928456

ABSTRACT

OBJECTIVE@#To explore the molecular reasons of weak expression of B antigen on the red cell.@*METHODS@#Serological test for blood group was carried out, including red cell and plasma grouping, and anti-A1 and anti-H testing, and confirming weak A or B antigens by adsorption and elution. Exons 1-7 were sequenced directly, and one of them was cloned and sequenced.@*RESULTS@#All of the 23 samples showed the weak B antigen by serological method. The alleles of the subgroups were identified by DNA sequencing, including 2 Bel subgroup, 4 B3 subgroup, 14 Bw subgroup, 2 CisAB subgroup and a novel allele. The novel allele showed a nucleotide substitution 662G>A in the exon 7, and the sequence was submitted to Blood Group Antigen Gene Mutation Database, and the novel allele was named Bel10.@*CONCLUSION@#Nucleotide substitution in exon results in blood subgroup, which showed that the antigens were weakened, and Bw phenotype was the most frequently subgroup.


Subject(s)
ABO Blood-Group System/genetics , Alleles , Exons , Genotype , Humans , Nucleotides , Phenotype
2.
Article in Chinese | WPRIM | ID: wpr-928032

ABSTRACT

Since the implementation of drug registration in China, the classification of Chinese medicine has greatly met the needs of public health and effectively guided the transformation, inheritance, and innovation of research achievements on traditional Chinese medicine(TCM). In the past 30 years, the development of new Chinese medicine has followed the registration transformation model of " one prescription for single drug". This model refers to the R&D and registration system of modern drugs, and approximates to the " law-abiding" medication method in TCM clinic, while it rarely reflects the sequential therapy of syndrome differentiation and comprehensive treatment with multiple measures. In 2017, Opinions on Deepening the Reform of Review and Approval System and Encouraging the Innovation of Drugs and Medical Devices released by the General Office of the CPC Central Committee and the General Office of the State Council pointed out that it is necessary to " establish and improve the registration and technical evaluation system in line with the characteristics of Chinese medicine, and handle the relationship between the traditional advantages of Chinese medicine and the requirements of modern drug research". Therefore, based on the development law and characteristics of TCM, clinical thinking should be highlighted in the current technical requirements and registration system of research and development of Chinese medicine. Based on the current situation of registration supervision of Chinese medicine and the modern drug research in China, the present study analyzed limitations and deficiency of " one prescription for single drug" in the research and development of Chinese medicine. Additionally, a new type of " series prescriptions" was proposed, which was consistent with clinical thinking and clinical reality. This study is expected to contribute to the independent innovation and high-quality development of the TCM industry.


Subject(s)
China , Drugs, Chinese Herbal/therapeutic use , Medicine, Chinese Traditional , Prescriptions , Public Health
3.
Article in Chinese | WPRIM | ID: wpr-927882

ABSTRACT

Air pollution has severe detrimental effects on public health.A substantial number of studies have demonstrated that air pollution exposure is a risk factor for the occurrence of cardiovascular and cerebrovascular diseases and a cause of non-communicable diseases.Both long-term and short-term exposure to air pollution are associated with respiratory diseases,stroke,coronary artery disease,and diabetes.Aiming to better understand the association,we reviewed the latest studies about the association of air pollution with cardiovascular and cerebrovascular diseases,especially stroke,coronary heart disease,arrhythmia,hypertension,and heart failure,and summarized the underlying mechanisms of the health damage caused by long-term and short-term exposure to air pollution.


Subject(s)
Air Pollutants/analysis , Air Pollution/adverse effects , Cardiovascular Diseases/etiology , Cerebrovascular Disorders/etiology , Environmental Exposure/analysis , Humans , Particulate Matter/analysis , Stroke/complications
4.
Chinese Acupuncture & Moxibustion ; (12): 1303-1307, 2021.
Article in Chinese | WPRIM | ID: wpr-921049

ABSTRACT

OBJECTIVE@#To observe the effect of nape cluster acupuncture on swallowing function and respiratory function in patients with post-stroke dysphagia, and to explore its relationship to cerebral arterial flow and neurotrophic factors.@*METHODS@#A total of 120 patients with post-stroke dysphagia were randomized into an observation group and a control group, 60 patients in each one. The conventional swallowing rehabilitation therapy and respiratory function training were adopted in the control group. On the basis of treatment in the control group, nape cluster acupuncture was applied at Fengchi (GB 20), Tianzhu (BL 10), Wangu (GB 12), Lianquan (CV 23), Panglianquan (Extra), once a day; pricking blood was applied at Jinjin (EX-HN 12) and Yuye (EX-HN 13), once every 2 days. Both groups were treated for 2 weeks. The therapeutic efficacy was compared between the two groups, and the swallowing function (scores of Kubota water swallowing test, standardized swallowing assessment [SSA] and video fluoroscopic swallowing study [VFSS]), the respiratory function indexes (forced vital capacity [FVC], maximal voluntary ventilation [MVV] and maximal expiratory time), the bilateral cerebral arterial hemodynamics parameters (systolic peak velocity [Vs], mean flow velocity [Vm] and vascular resistance index [RI]) and the serology indexes (brain-derived neurotrophic factor [BDNF], nerve growth factor [NGF] and insulin-like growth factors-1 [IGF-1]) before and after treatment were observed in the both groups.@*RESULTS@#The total effective rate was 80.0% (48/60) in the observation group, which was superior to 60.0% (36/60) in the control group (@*CONCLUSION@#On the basis of the conventional rehabilitation training, nape cluster acupuncture can effectively improve the swallowing function and respiratory function in patients with post-stroke dysphagia, its mechanism may be related to the improvement of cerebral hemodynamics and the regulation of neurotrophic factors.


Subject(s)
Acupuncture Points , Acupuncture Therapy , Deglutition , Deglutition Disorders/therapy , Humans , Treatment Outcome
5.
Journal of Breast Cancer ; : 428-442, 2021.
Article in English | WPRIM | ID: wpr-914831

ABSTRACT

Purpose@#Triple-negative breast cancer (TNBC) is a subtype of breast cancer. Increasing evidence supports that dysregulation of long noncoding RNAs (lncRNAs) plays a vital role in cancer progression. RNA component of mitochondrial RNA processing endoribonuclease (RMRP), a lncRNA, is characterized as a tumor-propeller in some cancers, but its mechanism in TNBC remains poorly understood. This study aimed to determine whether and how RMRP functions in TNBC. @*Methods@#Cell proliferation was determined by cell counting kit-8 (CCK-8) and colony formation assays and cell apoptosis by flow cytometry analysis and terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay. Cell migration and invasion were determined by transwell assays. RNA-binding protein immunoprecipitation (RIP), luciferase reporter, and RNA pulldown assays were implemented to assess the interaction of RMRP with other molecules in TNBC cells. @*Results@#RMRP expression was elevated in TNBC cells. RMRP knockdown repressed cell proliferation, migration, and invasion, but induced apoptosis in TNBC. In addition, RMRP was found to target microRNA-766-5p (miR-766-5p) in TNBC cells. Silencing miR-766-5p enhanced cell viability and decreased apoptosis, whereas miR-766-5p overexpression had opposite effects. Furthermore, miR-766-5p was found to bind to yes-associated protein 1 (YAP1). Moreover, miR-766-5p inhibition reversed the repressive effect of RMRP knockdown on the malignant progression of TNBC. @*Conclusion@#The present study manifested that RMRP promotes the growth, migration, and invasion of TNBC cells via the miR-766-5p/YAP1 axis. These findings provide novel perspectives for TNBC treatment.

6.
Article in Chinese | WPRIM | ID: wpr-912152

ABSTRACT

Objective:To evaluate endoscopic ultrasonography (EUS) in the diagnosis and preoperative assessment of hilar cholangiocarcinoma.Methods:Data of consecutive patients with hilar biliary strictures who underwent EUS and were confirmed by postoperative pathology from April 2016 to December 2019 were collected in the retrospective study. The clinical information, EUS image characteristics and lymph nodes of patients were recorded and analyzed.Results:A total of 58 patients were finally included in our study. Hilar cholangiocarcinoma of EUS image was characterized by heterogeneous hypoechoic, non-rich blood supply masses and (or) asymmetric thickening of bile duct wall. Among the 58 cases, 45 cases (77.6%) were manifested as masses and 32 cases (55.2%) were presented as thickening of bile duct wall. Nineteen cases (32.8%) had both manifestations above. There were 10 cases of vascular invasion detected by EUS scanning, including 3 cases of portal vein invasion, 4 cases of hepatic artery invasion, 3 cases of invasion of both loci. Postoperative pathology confirmed 14 cases of vascular invasion, with the diagnostic coincidence rate of 71.4% (10/14). A total of 101 lymph nodes were found in 53 patients by EUS scanning. The malignant lymph nodes presented hypoechoic, round or oval shape, and homogeneous echo. Compared with benign lymph nodes, malignant lymph nodes had higher morphological score (11.41±0.6 VS 9.01 ± 0.15, P<0.001), but there was no significant difference in size (13.29±0.90 mm VS 11.87±0.56 mm, P=0.28). According to the malignancy criteria of EUS lymph nodes (morphological score≥12), the accuracy, the sensitivity, the specificity, the positive predictive value and the negative predictive value of EUS for malignant lymph nodes were 92.1%(93/101), 76.5% (13/17), 95.2% (80/84), 76.5% (13/17) and 95.2% (80/84) , respectively. Conclusion:EUS can show the whole extrahepatic bile duct and part of intrahepatic bile duct, which is helpful to determine the location of tumor in the diagnosis of hilar cholangiocarcinoma. Moreover, EUS is helpful to diagnose hilar cholangiocarcinoma, which is of guiding significance in operative decisions.

7.
China Pharmacy ; (12): 2640-2646, 2021.
Article in Chinese | WPRIM | ID: wpr-904524

ABSTRACT

OBJECTIVE:To explore t he risk factors that may lead to the ineff ectiveness of using palonosetron combined with dexamethasone to prevent chemotherapy-induced nausea and vomiting (CINV),and to provide a reference for the rational choice and use of antiemetic drugs. METHODS :In a retrospective case-control study ,871 patients who used palonosetron combined with dexamethasone to prevent CINV in a tertiary cancer hospital from 2016 to 2020 were selected as the object. Totally 32 related data such as demographic data ,living habits ,medical history ,examination information and treatment information were counted as variables. Combined with single factor regression ,multi-factor regression, likelihood ratio forward or backward stepwise 163.com regression were used to comprehensively screen the factors for many times. The standard target factors screened by stepwise E-mail:kongtiandong@126.com regression were included in the multivariate Logistic regression analysis,and the regression model was evaluated by the ROC c urve. RESULTS :The multivariate Logistic regression model fitted well(AUC in ROC was 0.83,but 0.82 after screening ). The results showed that there were 15 statistically significant independent influential factors ,including 12 independent risk factors ,ie. poor nutritional status (OR=2.11,95%CI(1.05,4.22),P=0.036), history of gastrointestinal disease (OR=2.76,95%CI(1.87,4.07),P<0.001),abnormal electrolyte level (OR=2.54,95%CI (1.74,3.69),P<0.001),nausea and vomiting 24 h before chemotherapy (OR=8.47,95%CI(3.28,21.91),P<0.001),history of chemotherapy-induced vomiting (OR=3.20,95% CI (2.18,4.71),P<0.001),high risk level of vomiting caused by chemotherapy(OR=3.16,95%CI(2.38,4.20),P<0.001),application of opioid combined with non-steroidal analgesics (OR= 4.18,95%CI(2.06,8.49),P<0.001),the use of other drugs that stimulate the intestine and stomach (OR=2.49,95%CI(1.28, 4.83),P=0.007),history of surgery (OR=1.88,95%CI(1.34,2.63),P<0.001),high level of albumin (OR=1.05,95%CI (1.01,1.08),P=0.015),multiple days of single chemotherapy (OR=1.69,95%CI(1.11,2.56),P=0.014),and opioid analgesia medicine (OR=1.71,95%CI(1.15,2.53),P=0.007);and the following 3 independent protective factors included long time of diagnosis (OR=0.65,95%CI(0.46,0.93),P=0.019),non-first chemotherapy (OR=0.52,95%CI(0.33,0.83),P= 0.006),and drugs combined chemotherapy (OR=0.55,95%CI(0.34,0.90),P=0.018). CONCLUSIONS :Patients with the following conditions are more likely to experience CINV prevention ineffectiveness ,ie. single long-term chemotherapy ,application of chemotherapy plan with a higher risk of emesis ,history of chemotherapy-induced vomiting ,history of gastrointestinal diseases , nausea and vomiting 24 hours prior to chemotherapy ,history of surgery ,within 1 year of diagnosis ,chemotherapy for the first time,use of opioids ,use of 5-HT3 reuptake inhibitors ,malnutrition and electrolyte disorders.

8.
Article in Chinese | WPRIM | ID: wpr-848019

ABSTRACT

BACKGROUND: Digital impression technology has been widely used in the oral field. Compared with traditional impression technology, it is more convenient and accurate. OBJECTIVE: To explore the application of digital prosthesis for repairing palatal fistula in rabbits. METHODS: The rabbit models of palatal fistula were established by surgery. One month later, 10 rabbit models of palatal fistula were scanned by digital intraoral scanning system. The medical silicone rubber prosthesis for palatal fistula was manufactured by digital impression technology and digital design. After wearing the prosthesis, SUN LB, LAN YY, ZHOU HY, HUY. Application of digital prosthesis In me repair of palatal fistula In rabbits.  Zhongguo Zuzhi Gongcheng Yanjiu. 2020;24(14):2192-2196. DPI:10.3969/j.issn.2095-4344.2228 the accuracy, retention and sealing properties of the prosthesis were examined. The study was approved by the Laboratory Animal Ethical Committee of Hospital of Stomatology, Southwest Medical University. RESULTS AND CONCLUSION: After digital prosthesis repaired palatal fistula, the accuracy, retention and sealing properties of the prosthesis were good. To conclude, the digital prosthesis for palatal fistula repair by digital impression technology achieves accurate repair, and has certain clinical values.

9.
Article in Chinese | WPRIM | ID: wpr-888132

ABSTRACT

Xanthoceras sorbifolia, an excellent oil-rich woody species, has high comprehensive economic value in edible, medicinal, and ornamental fields. The chemical composition, pharmacological effect, and quality control of X. sorbifolia were introduced, and its development and application were reviewed in this study. As revealed by the previous research, the main chemical constituents of X. sorbifolia were triterpenoids, flavonoids, fatty acids, phenylpropanoids, steroids, phenolic acids, organic acids, etc. It possesses pharmacological effects, such as neuroprotection, bacteriostasis, anti-oxidation, anti-tumor, anti-inflammation, analgesia, anti-HIV, and anti-coagulation. X. sorbifolia is widely applied in medical, food, chemical industry, and other fields, and deserves in-depth research and development.


Subject(s)
Anti-Inflammatory Agents , Flavonoids , Research , Sapindaceae , Triterpenes
10.
Article in Chinese | WPRIM | ID: wpr-883551

ABSTRACT

Objective:To explore the application and effect of team-based learning (TBL) combined with flipped classroom in the teaching of physiology.Methods:A total of 70 medical students were selected and randomly divided into two groups, experimental group ( n=34) and control group ( n=36). Both groups studied digestion physiology by different method. In the experimental group, the students were taught by TBL combined with flipped classroom. The control group was given traditional teaching. The students studied and discussed problems in a team, and shared the answers in the flipped classroom. The effects of teaching were evaluated by the final test scores and the self-made questionnaire. SPSS 17.0 was used for t test on data comparison between the two groups. Results:The test scores of digestion physiology in the experimental group were (5.47±1.02) points, which were significantly higher than those of the control group (4.42±1.63) points, with significant differences ( P=0.020). A total of 34 questionnaires were issued and 34 valid questionnaires were collected, with an effective rate of 100%. The questionnaire results showed that TBL combined with flipped classroom was accepted and approved by about 82% of students in the experimental group who agreed that the teaching model helps enhance students' initiative and interest in learning, develop their sense of cooperation and comprehensive application ability. Conclusion:TBL combined with flipped classroom is feasible and effective in the physiological teaching, and it can be popularized in medical courses.

11.
Article in Chinese | WPRIM | ID: wpr-873282

ABSTRACT

Objective::To investigate the protective effect of salvianolic acid B on HepaRG hepatocyte injury induced by arsenic trioxide (As2O3 ) and its mechanism. Method::HepaRG cells were incubated with 5μmol·L-1 As2O3 for 24 h to induce hepatocyte injury. The cells were divided into control group, model group, salvianolic acid B 10 μmol·L-1 group, salvianolic acid B 10 μmol·L-1+ As2O3 group, salvianolic acid B 5 μmol·L-1+ As2O3 group, and salvianolic acid B 2.5 μmol·L-1+ As2O3 group. HepaRG cells were preincubated with salvianolic acid B for 2 h and then incubated with As2O3 for 24 h. At the end of the incubation, cell viability was detected by thiazolyl blue tetrazolium bromide assay, apoptosis was observed by Hoechst33342 fluorescence staining, apoptosis rate was detected by annexin V-FITC/propidium iodide double staining flow cytometry, and mitochondrial membrane was observed by JC-1 fluorescence staining. Western blot was used to detect the protective effect of expressions of relevant proteins Bcl-2, Bax, Akt, p-Akt on salvianolic acid B in the liver. Result::As2O3 concentration-dependently reduced the survival rate of HepaRG cells(P<0.01), salvianolic acid B had no effect on normal cell viability for 2 h, pre-incubation with salvianolic acid B(5, 10 μmol·L-1) for 2 h significantly increased the decreased cell survival rate caused by As2O3 (P<0.01). As2O3 significantly increased hepatocytes apoptosis rate(P<0.01), while pre-incubation with salvianolic acid B(10 μmol·L-1) deceased apoptosis rate(P<0.01). Incubation with As2O3 for 24 h caused decrease of mitochondrial membrane potential, pre-incubation with salvianolic acid B maintained mitochondrial membrane potential, indicating that the anti-apoptotic effect of salvianolic acid B were related to the mitochondrial pathway modulation. Western blot analysis showed that salvianolic acid B promoted the ratio of Bcl-2/Bax and promoted p-Akt/Akt compared with As2O3 group(P<0.01). Conclusion::Salvianolic acid B has a protective effect on hepatocyte injury induced by As2O3, and its mechanism is related to maintenance of mitochondrial function and inhibition of hepatocyte apoptosis.

12.
Article in Chinese | WPRIM | ID: wpr-873007

ABSTRACT

Shaoyao Gancaotang, first seen in Treatise on Febrile and Miscellaneous Diseases, is composed of Paeoniae Radix Alba and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle in equal proportion.It has the functions of preserving Yin to nourish blood, harmonizing liver and spleen, relieving spasm and pain.This formula is applied in leg and foot spasm and abdominal pain caused by blood deficiency, body fluid consuming and the unmoistened muscles and veins.It has been highly praised and used by medical experts throughout the ages and has extended its application scope.Modern pharmacological studies have shown that Shaoyao Gancaotang has significant effects in antispasmodic, analgesic, antitussive and other areas, and is used to treat spastic diseases, painful diseases, inflammatory diseases and so on.This paper will systematically elaborate the historical evolution, compatibility analysis, pharmacological and pharmacodynamic studies, modern clinical application of Shaoyao Gancaotang, in order to provide theoretical basis and reference for the development of this famous classical formula.

13.
Article in Chinese | WPRIM | ID: wpr-872746

ABSTRACT

Scutellarin is a flavonoid extracted from breviscapus, a traditional Chinese medicine. Pharmacological studies have shown that scutellarin has anti-inflammatory, antioxidant, anti-fibrosis, anti-tumor, improving cardiac and cerebral ischemia. In recent years, with the deepening of research on scutellarin, it was found that it could inhibit the tumor through multi-target and multi-pathway, and the anti-human colorectal cancer was related to the regulation of p53 pathway, Hedgelog pathway and erythropoietin generates liver cancer interactivator B2(EphrinB2).The anti-esophageal squamous cell carcinoma is related to protein kinaseB1 /protein kinaseB2( Akt1/Akt2).Anti-renal carcinoma and melanoma are associated with phosphatase and tension protein homologues(PTEN) and phosphatidylinositol 3-kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR) pathway. Anti-lung cancer is related to Akt/mTOR/4E binding protein1(4EBP1) and signal transduction and transcriptional activator(STAT3 )signaling pathway. Anti-cervical cancer is related to pyruvate kinase 2(PKM2).Anti-breast cancer is associated with Hippo/YAP pathway. At the same time, scutellarin was found to prevent diabetic microangiopathy, atherosclerosis and non-alcoholic fatty liver disease by regulating glucose and lipid metabolism, but the mechanism of action was not well studied. A review of the literature found that scutellarin anti-tumor, atherosclerosis, diabetic microangiopathy, non-alcoholic fatty liver disease, osteoarthritis, osteoporosis mechanism of action lack of detailed summary. In this paper, the research progress of pharmacological action and mechanism of scutellarin in recent 5 years is reviewed, and Suggestions on its current research status and future direction are put forward, in order to speed up the discovery of pharmacological mechanism of scutellarin and provide scientific basis for its further development and utilization.

14.
Clinical Medicine of China ; (12): 513-519, 2020.
Article in Chinese | WPRIM | ID: wpr-867575

ABSTRACT

Objective:To explore the related risk factors of stress hyperglycemia in patients with acute cerebral infarction and the effect of stress hyperglycemia on short-term cognitive function and prognosis of acute cerebral infarction.Methods:A prospective study was used to select non-diabetic acute cerebral infarction patients who were hospitalized in Department of Neurology, North China University of Science and Technology Affiliated Hospital from June 2016 to November 2019 for observation.According to the increase in blood sugar, the study subjects were divided into stress hyperglycemia group (107 cases) and normal blood sugar group (202 cases), record two groups of general information.After single-factor and multi-factor analysis, independent risk factors for stress hyperglycemia were screened.The degree of neurologic impairment and cognitive function were evaluated on admission and 30 days after onset of the disease in the two groups.The incidence of complications within 30 days after onset of the disease was recorded, and the outcome evaluation of 30 days after onset was completed.The incidence of adverse prognosis was compared between the two groups.Results:The incidence of stress hyperglycemia was 34.6%.By univariate analysis, the proportion of hypertension history, smoking history and multifocal infarction in stress hyperglycemia group was higher than that in normal blood glucose group (all P<0.05), and age, body mass index (BMI), national institute of health stroke scale(NIHSS) score, admission systolic blood pressure, total cholesterol, low density lipoprotein cholesterol and hypersensitive C-reactive protein (hs-CRP) in the blood glucose group were higher than those in the normal blood glucose group (all P<0.05). Multivariate logistic regression analysis showed that age, admission systolic blood pressure, BMI, NIHSS score and multifocal infarction were independent risk factors of stress hyperglycemia in patients with non-diabetic acute cerebral infarction (all P<0.05); the incidence of cognitive impairment in stress hyperglycemia group was higher than that in normal blood glucose group (21.8% vs.12.7%; χ 2=4.155, P=0.042), and the 30-day MOCA score was lower than that of the normal blood glucose group.According to multivariate Logistic regression analysis, stress hyperglycemia was independently associated with cognitive impairment in the 30 days after acute cerebral infarction( OR=1.788, 95% CI: 1.127-2.836, P=0.014). The results showed that the incidence of poor prognosis in stress hyperglycemia group was significantly higher than that in normal blood glucose group ( P<0.05); multivariate logistic regression analysis showed that stress hyperglycemia was independent of other factors related to disease outcome, and closely related to poor prognosis ( OR=1.824, 95% CI1.410-2.664, P=0.003). In addition, disease progression ( OR=2.208, 95% CI1.542-3.104, P<0.001) and severity of admission ( OR=2.340, 95% CI1.670-3.279, P<0.001) were also independent risk factors for poor prognosis. Conclusion:The occurrence of stress hyperglycemia after acute cerebral infarction in non-diabetic patients is the result of multiple factors.It is an independent influencing factor of poor prognosis, and can be used as one of the important reference indicators to predict the disease condition.

15.
Article in Chinese | WPRIM | ID: wpr-864256

ABSTRACT

Objective:To explore the clinical characteristics and treatment strategies of upper gastrointestinal perforation caused by ingested foreign bodies in children.Methods:A retrospective analysis of 32 patients with upper gastrointestinal perforation secondary to foreign bodies in Henan Children′s Hospital from January 2011 to February 2019 was performed.Some basic information was collected, including age, gender, type of foreign bodies, location of perforation, time of swallowing, clinical symptoms, treatment methods and outcomes.Results:Among the 32 children, there were 20 cases (62.5%) were male and 12 cases (37.5%) were female.The predilection age was 0 to <3 years old [21 cases (65.6%)]. The most common type of upper gastrointestinal foreign bodies was jujube pits [13 cases (40.6%)], followed by button batteries [10 cases (31.2%)], magnets [3 cases (9.4%)], fish bones [2 cases (6.3%)], lollipop stick [2 cases (6.3%)], button battery combined with magnet [1 case (3.1%)] and metal pin [1 case (3.1%)]. Majority of perforation was located in the esophagus [25 cases (78.2%)], especially in the upper esophagus [15/25 cases, (60.0%)]. The common symptoms of upper gastrointestinal perforation include fever [22 cases (68.8%)], dysphagia and/or salivation [21 cases (65.6%)], vomiting [24 cases (75%)], abdominal pain [5 cases (15.6%)] and expiratory dyspnea [1 case (3.1%)]. All the foreign bodies were successfully extracted by endoscopy [28 cases (87.5%)] and surgery [4 cases (12.5%)]. Moreover, all the patients recovered with both treatments of indwelling gastrointestinal decompression tube and enteral nutrition, including nasojejunal tube [24 cases (75.0%)], endoscopic hemoclip and nasojejunal nutrition [2 cases (6.3%)], and surgical operation [6 cases (18.7%)].Conclusions:The upper gastrointestinal foreign body is a common emergency in children, and might cause perforation, especially jujube pits, button batteries and magnets.The endoscopic procedure is safe and effective for the removal of foreign bodies.However, the surgical intervention is required as soon as possible due to the failure of endoscopic treatment.

16.
Article in Chinese | WPRIM | ID: wpr-863847

ABSTRACT

objective:To investigate the protective effect of oleanolic acid (OA) on the heart of diabetic cardiomyopathy (DCM) rats and its mechanism.Methods:Fifty healthy 4-week-old male SD rats were randomly (random number) divided into the normal control group (CON), diabetes mellitus group (DM), diabetes mellitus + oleanolic acid group (DM+OA), diabetes mellitus + chloroquine group (DM+CQ) and diabetes mellitus + oleanolic acid + chloroquine group (DM+OA+CQ), with 10 rats in each group. The diabetic animal model was established by feeding high-fat and high-sugar food (35.5% wt/ wt), intra-abdominal injection of streptozotocin (STZ) 50 mg/kg 4 weeks later, and feeding high-fat and high-sugar food for another 16 weeks. OA was orally administered at 80 mg/(kg·d) for 4 weeks, and CQ was intraperitoneal injected at 10 mg/kg per day for 4 weeks. The rats were anesthetized after echocardiographic examination at the end of the 20th week. Echocardiography was used to detect the heart function of SD rats, and the autophagy related proteins LAMP2, p-ULK1, p-p70s6k, p-raptor, LC3 and p62 were semi-quantitatively analyzed by Western blot. Changes in cardiac function and expression of autophagy related proteins in cardiac myocytes of rats were observed. One-way analysis of variance was used for comparison among groups of normally distributed measurement data, SNK- q test was used for pairwise comparison between two groups. Results:Compared with the CON group, the end diastolic wall thickness, left ventricular end diastolic volume, and end left ventricular systolic volume [(7.58±0.25) mm, (376.6±13.6) μL, and (132.6±10.8) μL] of the DM group were lower than those of the CON group [(8.37±0.11) mm, (458.3±16.4) μL, and (166.6±12.8) μL] (all P<0.05). Compared with the DM group, the end diastolic wall thickness, left ventricular end diastolic volume, and left ventricular end systolic volume [(8.63± 0.14) mm, (473.6±18.6) μL, and (174.6±12.7) μL] of the DM+OA group were higher than those of the DM group (all P<0.05). The anterior wall thickness, left ventricular end-diastolic volume, and left ventricular end-systolic volume [(7.97± 0.17) mm, (393.6±15.6) μL, and (147.6±10.2) μL] of the DM+OA+CQ group were lower than those of the DM+OA group (all P<0.05). The expression of autophagy-related protein p-ULK1 was increased and the expression of p-p70s6k and p-raptor was decreased in the DM group (all P<0.05). Compared with the DM group, cardiac dysfunction was reduced in the OA group, accompanied by decreased p62 expression and increased LC3Ⅱ/Ⅰ expression (both P<0.05). Compared with the DM+CQ group, p62 expression was decreased and LC3 Ⅱ/Ⅰ expression was increased in the DM+OA+CQ group (both P<0.05). Conclusions:OA has a protective effect on the myocardium of diabetic rats, which may be related to enhanced autophagy.

17.
Article in Chinese | WPRIM | ID: wpr-862694

ABSTRACT

<b>Objective::To study the protective effect and mechanism of Qidong Yixin oral liquid on doxorubicin-induced myocardial toxicity in mice. <b>Method::Ninety male ICR mice were randomly divided into normal group, model(DOX) group, DOX+ Qidong Yixin oral liquid group (9.55, 23.88, 47.75 g·kg<sup>-1</sup>) and high dose group (47.75 g·kg<sup>-1</sup>) with 15 mice in each group. The normal group and model group were given pure water by gavage, and each dose group of Qidong Yixin oral liquid was given different doses of Qidong Yixin oral liquid once a day for 21 days. On the seventh day, normal saline was injected into the abdominal cavity of the normal group and the high dose group of Qidong Yixin oral liquid. Doxorubicin was injected into the abdominal cavity of the other groups (15 mg·kg<sup>-1</sup>). After 21 days, the weight and heart weight of mice were weighed and cardiac index was calculated. Serum was taken for the detection of lactate dehydrogenase (LDH), creatine kinase (CK), aspartate aminotransferase (AST). Heart was taken for hematoxylin-eosin (HE) staining, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) in myocardial tissue were detected. The expression of nuclear factor NF-E2 related factor (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western blot. <b>Result::Compared with normal group, adriamycin could significantly reduce the body weight of mice (<italic>P</italic><0.01), increase the activities of LDH, CK and AST in serum(<italic>P</italic><0.01), and decrease the activities of antioxidant enzymes (<italic>P</italic><0.01). Compared with DOX group, high dose Qidong Yixin oral liquid could significantly increase the weight of mice (<italic>P</italic><0.05, <italic>P</italic><0.01), decrease the level of myocardial three enzymes(<italic>P</italic><0.01), increase the activity of antioxidant enzymes(<italic>P</italic><0.05, <italic>P</italic><0.01), and increase the expression of Nrf2 and HO-1(<italic>P</italic><0.01). <b>Conclusion::Qidong Yixin oral liquid has a good protective effect on doxorubicin myocardial toxicity. Its mechanism may be related to activating Nrf2/HO-1 signaling pathway and alleviating oxidative stress injury.

18.
Article in Chinese | WPRIM | ID: wpr-862681

ABSTRACT

Erigeron breviscapus, a species within the genus of Erigeron, is mainly distributed in Southwest China. It is cold in property, slightly bitter in taste, and has the effect of dispersing cold table, removing wind and dehumidification, promoting blood circulation and removing blood stasis, relieving pain and inflammation. Breviscapine is the extract of E. breviscapus. It is mainly consisted of flavonoids, lignans, coumarins, terpenes, phytosterols, etc. As the major components of breviscapine, the content of breviscapine b (4′-hydroxybaicalin-7-O-glucuronide) and breviscapine a (apigenin-7-O-glucuronide) is greater than 90%. Modern pharmacological studies have shown that breviscapine has a wide range of pharmacological effects, including anti-oxidation, anti-fibrosis, anti-inflammation, anti-aging, anti-platelet aggregation, lowering blood lipid, increasing blood flow, improving microcirculation, preventing and treating tumors, and resisting brain injury. In clinical, breviscapine has been widely used in the treatment of diabetes, cerebral insufficiency, sequelae caused by cerebral hemorrhage, hypermucolipemia, cerebral thrombosis, kidney disease, liver disease, Alzheimer's disease, and some other complex diseases. Specially, in the treatment of diabetes and its chronic complications, such as diabetic nephropathy, diabetic cardiomyopathy, diabetic foot, diabetic retinopathy, breviscapine has showed significant efficacy. In addition, studies have demonstrated that the combined application of breviscapine, mecobalamine, and micopol can improve the therapeutic effect. In this work, the application of breviscapine in the treatment of chronic complications of diabetes mellitus and its related combination drugs were reviewed, by which we attempted to provide some valuable clues for the clinical application of breviscapine in the treatment of diabetes mellitus and its chronic complications.

19.
Article in Chinese | WPRIM | ID: wpr-862669

ABSTRACT

Objective::To prepare 15 batches of Banxia Xiexintang substance benchmark and lyophilized powder from different places, and the lyophilized powder was analyzed by ultra-high performance liquid chromatography with diode array detection (UHPLC-DAD) and desorption electrospray ionization-mass spectrometry imaging (DESI-MSI) in order to investigate the advantages of DESI-MSI in quality control of famous classical formulas. Method::Taking Banxia Xiexintang as the research model, fingerprints of the substance benchmark and lyophilized powder were established by UHPLC-DAD, and the content of index components and the yield of dry extract were also investigated. Meanwhile, as the research carrier, the lyophilized powder corresponding to Banxia Xiexintang was dissolved in methanol and dotted on qualitative filter paper with 5 μL quantitative capillary, and fixed it on the slide to make samples. The samples were analyzed on a DESI-MSI system in positive and negative ion mode with methanol-formic acid (1 000∶1, flow rate of 3 μL·min-1) as spray solvent, N2 as spray gas (pressure of 0.5 MPa). The scanning range was 100-1 200 Da, the spatial resolution was 300 μm, the spray voltage was 3 kV, the sampling cone voltage was ±40 V, incidence angle of sprayer was 60 degree, its collection angle was 10 degree, the ion source temperature was 120 ℃. Result::DESI-MSI could not only detect the index components of liquiritin, baicalin and wogonoside, as well as the common peaks of liquiritin apioside, berberine and glycyrrhizic acid, but also analyzed them semi-quantitatively, the analysis results were basically consistent with UHPLC-DAD. At the same time, DESI-MSI could detect 16 other components from Glycyrrhizae Radix et Rhizoma, Coptidis Rhizoma, Scutellariae Radix, Jujubae Fructus and Ginseng Radix et Rhizoma, such as licoricesaponin G2, palmatine, coptisine, rutin and ginsenoside Rg1, and present their relative content visually. The qualitative analysis ability of DESI-MSI was much better than UHPLC-DAD. Conclusion::DESI-MSI can be used as the quality control method for substance benchmark and lyophilized powder and dispensing granules of famous classical formulas with advantages of high sensitivity, strong analytical ability, no complex sample processing, qualitative and relative content analysis of complex samples without reference substance.

20.
Article in Chinese | WPRIM | ID: wpr-861549

ABSTRACT

Objective: We aimed to compare mammography features between breast cancers patients older and younger than 60 years and to investigate the correlation between rate of microcalcification and age. Methods: A retrospective analysis was conducted on-mammography data of 1,959 patients with breast cancer diagnosed based on pathology findings from June 2012 to April 2013 at the Tianjin Medical University Cancer Institute and Hospital. Our study population was divided into 2 groups: older patient group (n=512) and younger patient group (n=1,447). Results: The proportions of cases involving high mass density were 73.0% (330/452) and 46.0% (464/1,008) in the older and younger patient groups, respectively. Furthermore, the proportions of cases involving equal or low mass density were 27.0% (122/ 452) and 54.0% (544/1,008) in the corresponding groups, respectively (P<0.05). The older and younger patient groups included 178 (34.8%) and 603 (41.7%) patients with microcalcification, respectively (P<0.05). We further divided our patient population (1,959 patients) into various age groups: <30, 30-39, 40-49, 50-59, 60-69, and ≥70 years. The proportions of cases involving microcalcification in the corresponding age groups were 58.8% (10/17), 42.8% (74/173), 41.0% (259/632), 41.6% (260/625), 38.2% (137/359), and 26.8% (41/153), respectively (P=0.008). Conclusions: Although the proportions of mass density and microcalcification were different between the older and younger patient groups, the fact that microcalcified foci decreased with age deserves further attention.

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