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1.
Intestinal Research ; : 224-230, 2022.
Article in English | WPRIM | ID: wpr-925131

ABSTRACT

Background/Aims@#Primary sclerosing cholangitis (PSC) is associated with inflammatory bowel disease (IBD). We aimed to evaluate the prevalence, clinical manifestation, and outcomes of PSC in Taiwanese patients with IBD. Methods: This retrospective study enrolled patients with IBD admitted from January 1, 1996, to December 31, 2018, to National Taiwan University Hospital. A case-matched analysis was performed comparing patients with IBD with and without PSC according to age, sex, and time of admission, with ratios of 1:4 and 1:2 in the adult and pediatric groups, respectively. @*Results@#In total, 763 patients with IBD were enrolled, 12 of whom were also diagnosed with PSC (1.57%). All these patients had ulcerative colitis (UC). A greater incidence of IBD with PSC was observed in younger patients than in older patients. Male sex was a risk factor for PSC in pediatric patients with IBD (P=0.015); 75% of these patients were diagnosed with PSC along with or after the diagnosis of UC. There was no significant difference in colitis extent and severity between the groups; however, a higher proportion of rectal sparing was observed in patients with PSC (P=0.001). There was no significant difference in cancer development between the groups (P=0.679). Conclusions: A 1.57% prevalence of PSC was observed in Taiwanese patients with IBD. The majority of patients with IBD and PSC were men and were diagnosed at a younger age. Hence, routine evaluation of biliary enzymes and liver imaging is recommended in young male patients with IBD.

2.
Gut and Liver ; : 396-403, 2022.
Article in English | WPRIM | ID: wpr-925029

ABSTRACT

Background/Aims@#Little is known about the clinical course of hepatitis B virus (HBV)-infected patients undergoing anti-tumor necrosis factor α (TNF-α) therapy for inflammatory bowel disease (IBD). We aimed to investigate the clinical course of HBV infection and IBD and to analyze liver dysfunction risks in patients undergoing anti-TNF-α therapy. @*Methods@#This retrospective multinational study involved multiple centers in Korea, China, Tai-wan, and Japan. We enrolled IBD patients with chronic or resolved HBV infection, who received anti-TNF-α therapy. The patients’ medical records were reviewed, and data were collected using a web-based case report form. @*Results@#Overall, 191 patients (77 ulcerative colitis and 114 Crohn’s disease) were included, 28.3% of whom received prophylactic antivirals. During a median follow-up duration of 32.4 months, 7.3% of patients experienced liver dysfunction due to HBV reactivation. Among patients with chronic HBV infection, the proportion experiencing liver dysfunction was significantly higher in the non-prophylaxis group (26% vs 8%, p=0.02). Liver dysfunction occurred in one patient with resolved HBV infection. Antiviral prophylaxis was independently associated with an 84% reduction in liver dysfunction risk in patients with chronic HBV infection (odds ratio, 0.16; 95% confidence interval, 0.04 to 0.66; p=0.01). The clinical course of IBD was not associated with liver dysfunction or the administration of antiviral prophylaxis. @*Conclusions@#Liver dysfunction due to HBV reactivation can occur in HBV-infected IBD patients treated with anti-TNF-α agents. Careful monitoring is needed in these patients, and antivirals should be administered, especially to those with chronic HBV infection.

3.
Chinese Journal of School Health ; (12): 1772-1775, 2021.
Article in Chinese | WPRIM | ID: wpr-906802

ABSTRACT

Objective@#To explore epidemiological characteristics and diagnosis delay among tuberculosis patients, and to provide reference basis for pulmonary tuberculosis prevention and control in schools.@*Methods@#Retrospective data of school based tuberculosis patients information and cluster epidemiological information in Hefei during Jan. 2019 to Dec. 2020 was collected. Changes of the epidemiological characteristics and diagnosis delay of school tuberculosis epidemic and possible role were explored.@*Results@#The reported incidence of Hefei school pulmonary tuberculosis was 14.04/10 5 in 2020. Two peaks of cases occurred during May to Jun. and Oct. to Nov. Teacher account for 8.00% of pulmonary tuberculosis cases in school, a significant increase was observed compared with 2019 ( χ 2=4.30, P <0.05). In 2020, the median length of treatment for cases reported by local medical institutions was 5.14 days, and the median length of diagnosis was 18 days, both of which were shorter than those in 2019 ( Z =22.45, 4.52, P <0.05). In multiple cases sporadic of school pulmonary tuberculosis, strong positive rate of PPD test was 13.50% among close contacts, and new case detectable rate was 0.62%. The median duration from exposure to symptoms onset among close contacts was 132 days, which significant increased compared to 2019 ( Z =251.50, P <0.05). The diagnosis delay among tuberculosis patients diagnosed by chest CT was 12.10%, and was 16.15% through supervision by school or parents. Chest radiograph was associated with higher risk of delayed diagnosis ( OR=4.34, P <0.05) compared to chest CT as the first medical radiology option. Low delayed diagnosis rate was associated with supervision of tuberculosis by school or parents than control ( OR=0.26, P <0.05).@*Conclusion@#Factors such as the selection of diagnostic radiology and case supervision are associated with delay diagnosis. It s necessary to strengthen the management and monitoring of the pulmonary tuberculosis epidemic in school.

4.
Article in Chinese | WPRIM | ID: wpr-906092

ABSTRACT

Objective:To explore the effect of natural decay of exogenously added fibrous roots on the growth and development of <italic>Paris polyphylla</italic> var. <italic>yunnanensis </italic>and its medicinal quality. Method:The effects of natural decay of fibrous roots at different amounts on mycorrhizal infection rate, physiological and biochemical indexes, and saponin contents of <italic>P. polyphylla</italic> var. <italic>yunnanensis </italic>were studied in pot culture experiments at room temperature. Result:The results showed that the infection rate of arbuscular mycorrhizal (AM) fungi in the root of <italic>P. polyphylla</italic> var. <italic>yunnanensis </italic>was not significantly affected by different fibrous root treatments, but there were significant differences in infection intensity. The photosynthetic pigment content in the leaves declined significantly with the increase in fibrous root amount, and the total chlorophyll was decreased by 78.7% at most. The contents of soluble protein, soluble sugar and malondialdehyde in the leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis </italic>showed an overall upward trend. The activities of the three protective enzymes varied. The peroxidase and malondialdehyde were reduced by 181.6% and 200.0% at most. In the root system of <italic>P. polyphylla </italic>var. <italic>yunnanensis</italic>, the contents of the above-mentioned six components decreased to varying degrees, with the largest reductions of peroxidase and malondialdehyde reaching 44.6% and 69.7%. Different fibrous root treatments resulted in a decrease in active component content of <italic>P. polyphylla </italic>var. <italic>yunnanensis</italic>. The total content of the four saponins was decreased by 58.9% at most, and the total saponin content by 46.9%. Conclusion:The natural decay of fibrous roots affects the soil microbial environment of root system, reduces the photosynthetic pigment content in leaves, and destroys the stability of cells, thus interfering with the growth and development of <italic>P. polyphylla </italic>var. <italic>yunnanensis</italic>, reducing its medicinal components, and causing continuous cropping obstacles.

5.
Article in Chinese | WPRIM | ID: wpr-887412

ABSTRACT

@#[摘 要] 目的:探究瑞戈非尼(regorafenib,Rego)对人肝癌SMMC-7721细胞增殖、凋亡的影响及其可能的机制。方法:将SMMC-7721细胞分为对照组及Rego组,分别用0、10 μmol/L Rego处理48 h后,流式细胞术检测各组细胞凋亡率,qPCR检测细胞中miR-122的表达。采用脂质体转染的方法将合成的hsa-miR-122-5p模拟物转染SMMC-7721细胞构建miR-122过表达的overExp-miR-122细胞,并将细胞分为对照组、Rego组、overExp-NC组、overExp-NC+Rego组、overExp-miR-122组及overExp-miR-122+Rego组,采用MTT法检测细胞活性,流式细胞术检测细胞凋亡率、WB法检测细胞中Bcl2、cleaved caspase-3、RAS、RAF1、p-ERK1蛋白表达水平。结果:与对照组相比,Rego处理后细胞凋亡率显著升高(P<0.05),且miR-122表达量显著上升(P<0.01);与overExp-NC组比较,overExp-miR-122组细胞增殖抑制率、凋亡率和cleaved caspase-3表达均显著升高(均P<0.01),RAS蛋白表达显著下降(P<0.05),Bcl2、RAF、p-ERK1蛋白表达均显著下降(均P<0.01);与overExp-miR-122组相比,overExp-miR-122+Rego组细胞中各检测指标变化进一步显著增加(P<0.01)。结论:Rego可抑制SMMC-7721细胞增殖、促进凋亡,其作用可能与调控miR-122、凋亡相关因子的表达和抑制RAS/RAF/ERK信号通路有关。

6.
Article in Chinese | WPRIM | ID: wpr-882193

ABSTRACT

@#[摘 要] 目的:探讨miR-125a-5p通过调控Bcl-2相关永生基因4(Bcl-2-associated athanogene 4,BAG4)的表达抑制胃癌细胞迁移和侵袭的分子机制。方法:选用2014年1月至2015年12月兰州大学第一医院手术切除的82例胃癌组织标本及配对的癌旁组织以及人胃癌细胞系MGC803、BGC823、SGC7901、HGC27及人胃黏膜上皮细胞(GES-1),qPCR法检测胃癌组织、癌旁组织及胃癌细胞系中miR-125a-5p的表达水平。分别将miR-125a-5p mimic、miR-125a-5p inhibitor、(si-BAG4)siRNA-BAG4及阴性对照质粒转染至胃癌细胞,划痕愈合实验和Transwell侵袭实验分别检测miR-125a-5p/BAG4信号轴对胃癌细胞迁移和侵袭能力的影响。WB检测胃癌细胞中BAG4蛋白的表达。荧光素酶报告基因实验验证miR-125a-5p和BAG4之间的靶向调控关系。结果:miR-125a-5p在胃癌组织和细胞系中均低表达(均P<0.01)。miR-125a-5p的表达与患者的性别(P=0.953)、年龄(P=0.772)、肿瘤部位(P=0.867)、组织学分级(P=0.745)和肿瘤大小(P=0.088)无相关性,与胃癌患者的T分期(P=0.003)、N分期(P=0.001)、M分期(P=0.027)和TNM分期(P=0.035)显著相关,差异有统计学意义。miR-125a-5p低表达是胃癌患者总生存时间的独立危险因素。过表达miR-125a-5p显著抑制胃癌细胞的迁移和侵袭能力(均P<0.01)。敲降BAG4可逆转miR-125a-5p inhibitor对胃癌细胞迁移和侵袭能力的抑制作用。荧光素酶报告基因实验证实miR-125a-5p可与BAG4 3'非翻译区(untranslated regions,UTR)结合抑制其表达。结论:miR-125a-5p通过靶向下调BAG4的表达水平进而抑制胃癌细胞的迁移和侵袭。

7.
Journal of Preventive Medicine ; (12): 250-254, 2021.
Article in Chinese | WPRIM | ID: wpr-876538

ABSTRACT

Objective@#To learn the awareness rate of adverse events following immunization ( AEFI ) among Chinese parents, so as to provide suggestions for promoting vaccination.@*Methods@#We searched relevant articles published before 24th June, 2020 from CNKI, Wanfang, VIP, CBM, PubMed and Web of Science, calculated the pooled awareness rate and 95% confidence interval ( CI ) , conducted Egger's test for publication bias and sensitivity analysis for stability of results. @*Results@#Eight articles using cross-sectional design were included after screening from 235 initial records. Among 5 433 subjects, the pooled awareness rate of AEFI was 66.76% ( 95%CI: 52.75%-78.33% ) . Non-immigrant population possessed a higher awareness rate ( 67.32% ) compared with the immigrant population ( 56.54% ) . The parents with different levels of education showed various awareness rate of AEFI ( P<0.05 ) . The awareness rates of "children should be observed for at least 30 minutes after vaccination","slight adverse effects were commonly seen after vaccination","local redness and induration might occur after diphtheria-tetanus-pertussis ( DTP ) immunization","polio vaccine might bring mild diarrhea" were 86.18%, 66.76%, 41.89% and 30.22%, respectively. Egger's test showed that there was no publication bias. Sensitivity analysis indicated that the results were robust.@*Conclusion@#The pooled awareness rate of AEFI among Chinese parents is 66.76%, with lower rates found in the parents who are immigrants and have lower level of education.

8.
Article in English | WPRIM | ID: wpr-875153

ABSTRACT

Background@#Goslings in several Taiwanese farms experienced gosling feather loss disease (GFL) at 21–35 days and goose broke feather disease (GBF) at 42–60 days. The prevalence ranges from a few birds to 500 cases per field. It is estimated that about 12,000 geese have been infected, the morbidity is 70–80% and the mortality is 20–30%. @*Objectives@#This study aims to investigate the pathogens that cause GFL and GBF. Focus on the study of the correlation between goose circovirus (GoCV) and goose parvovirus (GPV) with the goose feather loss in southern Taiwan. Furthermore, a phylogenetic tree was established to align the differences between southern and northern Taiwan and compare with virus strains from China and Europe. @*Methods@#Samples were collected from animal hospitals. Molecular and microscopy diagnostics were used to examine 92 geese. Specific quantitative polymerase chain reaction (Q-PCR) assays are performed to evaluate GPV and GoCV viral loads and simultaneously evaluated the feather loss conditions in geese with the scoring method. @*Results@#High prevalence of GoCV and GPV infection in geese showing signs of GFL and GBF. Inclusion body was detected in the feather follicles and Lieberkühn crypt epithelial cells. The Q-PCR showed the high correlation between feather loss and viruses during 3rd– 5th week. However, the infection was not detected using the same test in 60 healthy geese. @*Conclusions@#Thus, GFL and GBF appear to be significantly closely related to GoCV and GPV. The geese feathers showed increasing recovery after being quarantined and disinfected.

9.
Chinese Journal of School Health ; (12): 120-123, 2021.
Article in Chinese | WPRIM | ID: wpr-862610

ABSTRACT

Objective@#To evaluate the dynamic prevalence of dental fluorosis of children and levels of fluoride in drinking water after improvement of water in Xi an City, to provide scientific basis for water fluoridation improvement.@*Methods@#A total of 35 fluorosis endemic villages were selected as fixed monitor sites in 2014-2018, the ways of water improvement were surveyed, water fluorine content were detected and the prevalence of dental fluorosis in children aged 8 to 12 years were examined.@*Results@#Rates of excess fluoride in drinking water from 2014 to 2018 were 22.86%, 14.29%,11.43%, 11.43% and 8.57%, the difference were significant(χ2=16.44, P<0.01).The dental fluorosis detection rates of children aged 8 to 12 years were 20.89%,18.22%,17.46%,18.13% and 16.76% in 2014-2018 which showed a obvious descending trend by year(χ2=10.02, P<0.01). The detection rate of dental fluorosis in children aged 8 and 9 years showed a decreasing trend by year(χ2=6.53, 4.54, P<0.05).The difference of total rate of dental fluorisis,rate of mild cases rate of moderate-to-severe cases were statistically between the villages without qualified water and the villages with normal fluorine water(χ2=179.22, 167.93,10.35, P<0.01). The rate of detection in the villages with the water fluorine exceed standard in 2014-2018 showed a declining trend year by year(χ2=28.50, P<0.01). The detection rate were significant different across water improvement methods(χ2=197.76, P<0.01). Detection rate of dental fluorosis decreased from 2014 to 2018 in the areas with municipal water supply showed a decreasing after year(χ2=12.16, P<0.01).@*Conclusion@#The improvement of municipal water supply shows significant effects on water fluorosis control, the detection rate of water fluoride and children s dental fluorosis in some villages with the other ways of water improvement are still higher than expected, the continuously monitor of fluoride content in water and dental fluorosis in children should be strengthened.

10.
J Biosci ; 2020 Oct; : 1-7
Article | IMSEAR | ID: sea-214221

ABSTRACT

Herein, we found that serum concentration of superoxide dismutase 3 (SOD3) was significantly reduced inchildren with mycoplasma pneumonia (MP) infection. To study the roles of SOD3 in inflammatory regulationof MP infection, human A549 type II alveolar epithelial cells were stimulated with 107 CCU/ml of MP to buildMP infection in vitro. Secretion of pro-inflammatory cytokine interleukin (IL)-8 and tumor necrosis factor(TNF)-a were measured via enzyme-linked immunosorbent assay (ELISA) to assess the inflammatory responseof A549 cells. Levofloxacin (LVFX) was used as an anti-inflammatory drug while recombinant TNF-a wasused as an inflammatory promotor in MP-infected cells. Transcriptional activity of nuclear factor (NF)-rB wasassessed by detecting protein levels of nuclear NF-rB and cytoplasm NF-rB using Western blot analysis. Ourdata suggested that the expression of SOD3 mRNA and protein, as well as content of SOD3 in culturedsupernatant, were time-dependently inhibited in MP-infected A549 cells. However, lentiviruses-mediatedSOD3 overexpression alleviated inflammatory response of MP-infected A549 cells, and prevented the uncleartranslocation of NF-rB, as evidenced by obviously reducing the production of IL-8 and TNF-a in cell culturedsupernatant, as well as decreasing nuclear NF-rB while increasing cytoplasm NF-rB. Inspiringly, SOD3overexpression induced anti-inflammatory effect and the inactivation of NF-rB was similar to that of 2 lg/mlof LVFX, but reversed by additional TNF-a treatment. Therefore, we can conclude that transcriptional activityof NF-jB was the underlying mechanism, by which SOD3 regulated inflammatory response in MP infectionin vitro

11.
Electron. j. biotechnol ; 47: 1-9, sept. 2020. graf, tab
Article in English | LILACS | ID: biblio-1224606

ABSTRACT

BACKGROUND: γ-Aminobutyric acid (GABA) bypasses the TCA cycle via GABA shunt, suggesting a relationship with respiration. However, little is known about its role in seed germination under salt conditions. RESULTS: In this study, exogenous GABA was shown to have almost no influence on mungbean seed germination, except 0.1 mM at 10 h, while it completely alleviated the inhibition of germination by salt treatment. Seed respiration was significantly inhibited by 0.1 and 0.5 mM GABA, but was evidently enhanced under salt treatment, whereas both were promoted by 1 mM GABA alone or with salt treatment. Mitochondrial respiration also showed a similar trend at 0.1 mM GABA. Moreover, proteomic analysis further showed that 43 annotated proteins were affected by exogenous GABA, even 0.1 mM under salt treatment, including complexes of the mitochondrial respiratory chain. CONCLUSIONS: Our study provides new evidence that GABA may act as a signal molecule in regulating respiration of mungbean seed germination in response to salt stress.


Subject(s)
Seeds/growth & development , Vigna , gamma-Aminobutyric Acid , Respiration , Stress, Physiological , Proteins , Germination , Proteomics , Salt Tolerance , Salt Stress
12.
Int. braz. j. urol ; 46(supl.1): 19-25, July 2020. graf
Article in English | LILACS | ID: biblio-1134294

ABSTRACT

ABSTRACT Although urological diseases are not directly related to coronavirus disease 2019 (COVID-19), urologists need to make comprehensive plans for this disease. Urological conditions such as benign prostatic hyperplasia and tumors are very common in elderly patients. This group of patients is often accompanied by underlying comorbidities or immune dysfunction. They are at higher risk of COVID-19 infection and they tend to have severe manifestations. Although fever can occur along with urological infections, it is actually one of the commonest symptoms of COVID-19; urologists must always maintain a high index of suspicion in their clinical practices. As a urological surgeon, how we can protect medical staff during surgery is a major concern. Our hospital had early adoption of a series of strict protective and control measures, and was able to avoid cross-infection and outbreak of COVID-19. This paper discusses the effective measures that can be useful when dealing with urological patients with COVID-19.


Subject(s)
Humans , Male , Aged , Pneumonia, Viral/epidemiology , Urologic Diseases/complications , Coronavirus Infections/epidemiology , Pneumonia, Viral/prevention & control , Urologic Diseases/diagnosis , Urologic Diseases/therapy , China , Coronavirus Infections/prevention & control , Betacoronavirus , SARS-CoV-2 , COVID-19 , COVID-19/prevention & control
13.
Article in Chinese | WPRIM | ID: wpr-837763

ABSTRACT

Epidemic infectious diseases have become a major threat to public health safety. This paper reviews the previous researches and analyzes the psychological impact of infectious disease outbreak on medical staff, patients and the public, exploring the risk and protective factors of adverse psychological reactions, so as to provide references for future research and counseling after coronavirus disease 2019 outbreak. It is suggested that multi-center and large-scale follow-up investigation is needed, and more attention needs to be paid on influences of stigma and public opinion on internet and the change of positive psychological quality after the epidemic outbreak. Moreover, the research results of risk and protective factors should be fully used in psychological intervention.

14.
Gut and Liver ; : 685-698, 2020.
Article in English | WPRIM | ID: wpr-833161

ABSTRACT

The advent of biologics and biologic therapy has transformed the management of inflammatory bowel disease (IBD) with enhanced early and adequate responses to treatment, fewer hospitalizations, a reduced need for surgery, and unprecedented outcomes including complete mucosal and histologic healing. However, an important issue with the use of anti-tumor necrosis factor (anti-TNF) agents in IBD is the increased risk of tuberculosis (TB). This is compounded by the diagnostic dilemma when differentiating between Crohn’s disease and gastrointestinal TB, and the potentially serious consequences of initiating an incorrect treatment in the case of misdiagnosis. The interplay between IBD and TB is most relevant in Asia, where more than 60% of the 10.4million new TB cases in 2016 were reported. A number of studies have reported an increased risk of TB with anti-TNF agents, including in patients who had tested negative for TB prior to treatment initiation. The limited evidence currently available regarding adhesion molecule antagonists such as vedolizumab suggests a comparatively lower risk of TB, thus making them a promising option for IBD management in TBendemic regions. This comprehensive review examines the available literature on the risk of TB with the use of biologics in the TB-endemic regions of Asia, focusing on the diagnostic dilemma, the risk of reactivation, and the optimized management algorithms for latent and active disease.

15.
Article in English | WPRIM | ID: wpr-829495

ABSTRACT

@#A novel strain of coronavirus was first detected in Wuhan, China in December 2019. It is the causative agent of a respiratory disease known as the Coronavirus Disease 2019 (COVID-19). On 12th March 2020, it was declared as a global pandemic by the World Health Organization. The aim of the study was to study the impact of MCO in terms of the academic performance and finance on the student population and to measure the level of knowledge, attitude and practice towards prevention of coronavirus disease 19 (COVID-19) among a private university students in Malaysia. On 22nd April until 30th April 2020, a cross-sectional study using a convenient sampling method was conducted among 401 students using a self-administered online questionnaire survey. It was found that 272 (67.8%) of the respondents had a less negative impact of MCO while 129 (32.3%) of the respondent had a more negative impact of MCO. The findings show also 229 respondents (57.1%) have adequate knowledge. As for the attitude, about 237 respondents (59.1%) have an adequate attitude and only 191 respondents (47.6%) have adequate practice towards the prevention of COVID-19. There was a significant relationship between knowledge, attitude, and practice with family income, a significant relationship between knowledge and practice, a significant relationship between knowledge and attitude, a sig. relationship between knowledge, attitude, practice with university faculties, the relationship between knowledge and source of information. The MCO showed significant association with knowledge level and with practice, also MCO was less negatively impact on the medical faculty students with p-value 0.004. The MCO was more negatively impacting those with less family income. The study findings concluded that the majority of the participants had a fair level of knowledge, attitude, and a lesser level of good practice towards the prevention of COVID-19. However, the COVID-19 is still considered a global pandemic and has not resolved yet. Therefore, to minimize the risk of future COVID-19 cases current efforts to educate the public towards knowledge, attitude, and practice towards prevention of COVID-19 in public health should be done.

16.
Article in Chinese | WPRIM | ID: wpr-847391

ABSTRACT

BACKGROUND: Studies have confirmed that inflammatory factors secreted by synovitis that accelerate the catabolism of articular cartilage have become the main cause of osteoarthritis. Matrix metalloproteinase 3 and matrix metalloproteinase 13 play a key role in osteoarthritis. OBJECTIVE: To study the effective mechanism of platelet-rich plasma (PRP) in the treatment of osteoarthritis. METHODS: The rat knee synovial cells and chondrocytes were isolated and extracted separately. The blood samples of rats were extracted to prepare PRP preparation. Then, synovial cells were divided into a control group and an E. coli lipopolysaccharide (LPS) treatment group, where synovial cells were stimulated with LPS to create a synovitis model. The synovitis cells were further divided into a PRP treatment group and an untreated group. The cells in each group were cultured for 24 hours. A portion of the medium was taken, in which the levels of interleukin-1β, interleukin-6 and tumor necrosis factor-α was detected using ELISA. The other part of the medium was used to treat chondrocytes. After 48 hours of culture, the changes of type I, II collagens, matrix metalloproteinase 3 and matrix metalloproteinase 13 protein levels in chondrocytes were detected by western blot. The expression levels of matrix metalloproteinase 3 and matrix metalloproteinase 13 mRNA were detected by real-time PCR. RESULTS AND CONCLUSION: Determined by the ELISA, the levels of interleukin-1β, interleukin-6 and tumor necrosis factor-α in the synovial cell culture medium of the LPS treatment group were significantly higher than those of the control group and the PRP treatment group (P < 0.01). The levels of interleukin-1β, interleukin-6 and tumor necrosis factor-α in the medium of PRP treatment group were significantly higher than those in the control group (P < 0.05). Western blot showed that the expression of type I and II collagens in the chondrocytes of the PRP treatment group were significantly higher than those in the control and LPS treatment groups (P < 0.01); the expression of type I and II collagens in the chondrocytes of the LPS treatment group was significantly lower than that in the control group (P < 0.05). The expression levels of matrix metalloproteinase 3 and matrix metalloproteinase 13 proteins in the LPS treatment group were significantly higher than those in the PRP treatment and control groups (P < 0.01). PCR analysis showed that the mRNA expression of matrix metalloproteinase 3 and matrix metalloproteinase 13 in the LPS treatment group was significantly higher than that in the PRP treatment and control groups. The findings of this study reveal that PRP treatment can reduce interleukin-1β, interleukin-6 and tumor necrosis factor-α levels in synovial cells, decrease matrix metalloproteinase 3 and matrix metalloproteinase 13 protein and mRNA levels in chondrocytes, and increase type I and II collagen expression, thereby protecting the cartilage.

17.
Chinese Journal of School Health ; (12): 889-892, 2020.
Article in Chinese | WPRIM | ID: wpr-822534

ABSTRACT

Objective@#To explore determinants of childhood trauma among college students with left-behind experience, and to provide a reference for effective intervention among students with left-behind experience.@*Methods@#A total of 2 468 students selected from 5 universities and 2 higher vocational colleges in tianjin by stratified cluster sampling method were investigated by self-compiled questionnaire and childhood trauma questionnaire.@*Results@#The scores in emotional abuse, sexual abuse, emotional neglect, physical neglect and childhood trauma of students with left-behind experience were significantly higher than those without left-behind experience(t=3.01,3.13,3.24,2.27,3.60,P<0.05);parental separation times and the frequency of parental return had significant interaction effect on the total score of childhood trauma of students with left-behind experience (F=2.37, P<0.05);the gender had a significant major effect on the total score of childhood trauma of students with left-behind experience under the interaction with the place of origin, age at first separation,the cumulative time of leftbehind experiences and the frequency of parents contacting (F=4.49,5.23,5.93,5.11,P<0.05);the age of subjects when parents going out under the interaction with the place of origin, the gender, if only-child,parental separation times and the frequency of parental return;as well as the frequency of parents contacting under the interaction with the place of origin,the household registration, the gender, if only-child and the cumulative time of left-behind experiences also had significantly main effect(F=3.88,4.25,3.32,2.86,3.45;3.82,4.02,2.64,3.29,P<0.05).@*Conclusion@#It is necessary to attach great importance to demographic and context information regarding left-behind experiences,which lead to more specific and effective prevention and intervention strategy for individual with left-behind experiences.

18.
Article in Chinese | WPRIM | ID: wpr-879323

ABSTRACT

OBJECTIVE@#To compare clinical effect of movable external fixation and fusion fixation for the treatment of elbow tuberculosis.@*METHODS@#From October 2013 to June 2019, 52 patients with elbow tuberculosis treated with standard antituberculosis therapy were divided into movable external fixation group and fusion fixation group according to treatment methods. In group A, there were 25 patients, including 11 males and 14 females, aged from 24 to 75 years old with an average of (42.81± 9.01) years old; the courses of diseases ranged from 2 to 9 months with an average of (3.96±1.45) months. In group B, there were 27 patients, including 15 males and 12 females, aged from 23 to 77 years old with an averageof (44.08±7.44) years old; the courses of diseases ranged from 2 to 7 months with an average of (3.88±1.67) months. All patients were performed focus debridement. Intraoperative blood loss, operative time were compared between two groups. VAS score before operation, 2 weeks and 12 months after operation were applied to evaluate pain relieve;Mayo elbow performance score (MEPS) before operation, 1 and 12 months after operation were used to evaluate clinical effect;changes of erythrocyte sedimentation rate (ESR) and Creactive protein, CRP) before operation, 3 weeks after antituberculosis therapy, 1 week and 6 months after operation were compared between two groups.@*RESULTS@#All patients were followed up from 12 to 20 months with an average of (13.50±4.85) months. No mixed infection and recurrence of tuberculosis occurred. There were no statistical differences in intraoperative blood loss and operative time(@*CONCLUSION@#For elbow tuberculosis, movable external fixation and fusion fixation have equal effect in operative time, amount of bleeding and control of tuberculosis infection indicator. Movable external fixation need earlier functional exercise, not conducive to pain relief at early stage, which may be better than fusion fixation, it is worth clinical promoting.


Subject(s)
Elbow , Elbow Joint/surgery , External Fixators , Female , Fracture Fixation , Humans , Infant , Male , Treatment Outcome , Tuberculosis/surgery
19.
Chinese Journal of School Health ; (12): 1824-1826, 2020.
Article in Chinese | WPRIM | ID: wpr-862207

ABSTRACT

Objective@#To analyze the consistency between self-reported oral health and proposed standard in evaluating children oral health status, and to test whether self-reported oral health can be used in children population.@*Methods@#The clinical examination data and questionnaire data were 2 158 children aged 12-year-old in Beijing, China. Child oral health status was measured by self-reported oral health and proposed standard from World Health Organization (WHO), and was classified into "good, general, poor" based on each method. Kappa test was used to evaluate the consistency of two measurements. Using multivariate nonordinal logistic regression to identify the influencing factors of self-reported oral health, and to explore the possible reasons for the consistency differences of two measurements.@*Results@#According to the WHO standard, 25.5%, 65.3% and 9.2% children oral health were rated as good, general and poor, respectively. The proportion was 50.6%, 37.7% and 11.8% respectively based on self-report method. Kappa value for two measurements was 0.04, indicating poor agreement between two methods. Results from regression model show that both the untreated caries (OR=2.47, P<0.01) and toothache (OR=3.54, P<0.01) were associated with self-reported oral health, while not observed in periodontal health (OR=1.34, P=0.18) and oral hygiene status(OR=0.95, P=0.75).@*Conclusion@#Self-reported oral health results can reflect caries status to some extents among children, but it failed to reflect other aspects, such as periodontal health status and oral hygiene. Self-reported oral health among children should be considered based on the requirements of assessment content and accuracy in the future work.

20.
Braz. j. med. biol. res ; 53(8): e9469, 2020. tab, graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1132537

ABSTRACT

This is a retrospective, single-center observational study to explore the predictors of chest drainage for neonatal pneumothorax. A total of 183 neonates (age ≤28 days) who presented to the Children's Hospital of Soochow University between January 1, 2015 and December 31, 2018 for pneumothorax or developed pneumothorax during a hospital stay were included. Demographic data, clinical presentation, and imaging characteristics of neonatal pneumothorax were collected and analyzed. We used univariate and multivariate logistic regression analyses to determine significant predictors of chest drainage of pneumothorax in neonates. Pneumothorax occurred within 24 h after birth in 131 (71.6%) cases, between 24 and 48 h after birth in 41 (22.4%) cases, and 48 h after birth in 11 (6.0%) cases. Univariate and multivariate logistic regression analyses revealed that lung collapse ≥1/3 on initial chest X-ray (OR 4.99, 95%CI 2.25-11.07), chest retractions (OR 8.12, 95%CI 2.88-22.89), cyanosis (OR 2.25, 95%CI 1.08-4.66), and frothing from mouth (OR 2.49, 95%CI 1.12-5.49) (P<0.05 for all) were significant predictors of the need for chest drainage due to pneumothorax. In conclusion, the thorough evaluation of the above predictive factors can guide treatment and improve patient outcome.


Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Pneumothorax , Meconium Aspiration Syndrome , Retrospective Studies , Dyspnea , Length of Stay
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