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1.
Article in Chinese | WPRIM | ID: wpr-701264

ABSTRACT

Objective To master the condition of drinking water fluorosis in Jiangsu Province and the operation of improved-water project,and to provide a scientific basis for further prevention and treatment of drinking water fluorosis.Methods In 2015,17 drinking water fluorosis counties were selected in Jiangsu Province,and 3 villages in each county were selected as monitoring sites,when the number of drinking water fluorosis villages was less than 3,all of the drinking water fluorosis villages were selected as monitoring sites.To know the progress and running conditions of the improved-water facilities,water samples were collected to determine the fluorosis level.All children aged 8-12 years were monitored for dental fluorosis.Two monitoring counties were selected to monitor the X-ray changes and urinary fluoride of adults with skeletal fluorosis.Results A total of 47 villages were surveyed,46 of which had water improved,and 1 had water unimproved.The rate of water improved was 97.87% (46/47).The number of improved-water facilities that were operating normally was 45,and the rate was 97.83% (45/46).The number of intermittent operation was 1.The number of improved-water facilities with qualified water fluoride was 42,and the rate was 91.30% (42/46).In the villages with normal improved-water facilities,and qualified water fluoride,the total rate of dental fluorosis of children aged 8 to 12 was 15.56% (600/3 855),and the dental fluorosis index was 0.29.In the villages with normal improved-water facilities,but unqualified water fluoride,the total rate of dental fluorosis of children aged 8 to 12 was 57.30% (267/466),and the dental fluorosis index was 1.21.The range of water fluoride in water unimproved villages was 1.2-2.0 mg/L,and the total rate of dental fluorosis of children aged 8 to 12 was 40.91% (36/88),and the dental fluomsis index was 0.66.The detection rate of skeletal fluorosis in adults was 5.16%(16/310),the geometric mean of urinary fluoride was 0.67 mg/L,and the range of urinary fluoride was 0.02-2.76 mg/L.Conclusions The improved-water projects in drinking-water fluorosis areas in Jiangsu Province have achieved a certain effect,but water fluoride is unqualified in some water projects.We should strengthen the maintenance and supervision of the water projects to ensure the normal operation of water projects.

2.
Chinese Journal of Endemiology ; (12): 596-598, 2015.
Article in Chinese | WPRIM | ID: wpr-480236

ABSTRACT

Objective To know the state of endemic fluorosis,running state of water improvement project in Jiangsu Province,and to provide a scientific basis for adjusting control measures against the disease.Methods Nine key counties were selected from the endemic fluorosis areas as survey spots in Jiangsu in 2012.Three villages in each spot were selected as survey villages.To known the progress and running conditions of water facilities,water samples were collected to determine the fluorosis level.All children aged 8 to 12 were tested for dental fluorosis at the survey villages.The fluorine content in water was determined with F-selective electrode (WS/T 106-1999).Dental fluorosis was diagnosed byased on the Dean method.Results Twenty-seven villages were investigated,the number of villages that had water quality improved was 26,the rate was 96.30% (26/27);the number of villages that did not have water quality improved was 1,the rate was 3.70% (1/27).Among the 26 facilities,21 improved-water facilities ran normally,the rate was 80.77% (21/26);2 facilities ran with intermittently water supply,the rate was 8.70% (2/26);3 facilities were scraped,the rate was 11.54% (3/26).In the villages with normal improved-water facilities and qualified water fluorosis,the total rate of dental fluorosis of children aged 8 to 12 was 22.74% (380/1 761),the index of dental fluorosis was 0.45.In the villages with abnormal improved-water facilities and excessive water fluorosis,the total rate of dental fluorosis of children aged 8 to 12 was 66.51% (143/215),the index of dental fluorosis was 1.45.The total rate of dental fluorosis of children aged 8 to 12 was 42.86% (12/28) in the villages which had not changed water source,the index of dental fluorosis was 0.86.Conclusion Improvement of water decreasing fluorine project in Jiangsu Province has a certain effect on children's dental fluorosis,but some of the water fluorosis projects are scrapped,and excess fluorine water should be taken seriously.

3.
Chinese Journal of Endemiology ; (12): 142-144, 2015.
Article in Chinese | WPRIM | ID: wpr-470376

ABSTRACT

Objective To evaluate the effect of health education on endemic fluorosis in Jiangsu Province,and to provide reference data for the further prevention and control.Methods In 2012,according to the past prevalence of endemic fluorosis in Jiangsu Province,a total of 12 counties (districts) were selected,and 3 townships were chosen in each project county.Health educational activities were carried out iu the classes of grade 4-6 in the central primary school.In the meantime,3 villages were chosen in each selected township where the health educational activities in the community were carried out.Before and after the health education activitics,thirty students in one class of the fifth grade in the central primary school were extracted,and 15 housewives near the central primary school were extracted too.Questionnaire surveys on knowledge of endemic fluorosis were conducted in each project country.Results After the health educational activities,the number of questions of knowledge awareness in the students and the housewives was 3 240,1 620; the number of correct answer was 3 101,1 501;the rates were 95.71% and 92.65% respectively,and increased significantly compared with those before the intervention [65.52% (2 123/3 240),68.64% (1 112/1 620)],the differences were significant (x2=944.480,299.160,all P < 0.05).Before the health educational activities,the rate of knowledge awarenes in the students was lower than that in the housewives (x2 =4.714,P < 0.05).After the health educational activities,the rate of knowledge awareness in the students was higher than that in the housewives,the difference was significant (x2 =20.055,P < 0.05).Conclusion The rates of knowledge awareness in the students and the housewives are increased significantly after the health educational activities,and the expected goal is reached.

4.
Article in Chinese | WPRIM | ID: wpr-474142

ABSTRACT

BACKGROUND:Exosomes are membrane vesicles secreted by mesenchymal stem cells. Increasing studies have shown that mesenchymal stem cells can secrete exosomes via paracrine function to play a role in tissue injury. However, reports on how to isolate and identify exosomes derived from human umbilical cord blood mesenchymal stem cells are few. OBJECTIVE:To extract, purify and identify exosomes derived from human umbilical cord blood mesenchymal stem cells. METHODS:The cellculture supernatant of human umbilical cord blood mesenchymal stem cells was col ected. Exosome was extracted and purified with ultrafiltration and gradient centrifugation methods. The morphology of exosome was observed by transmission electronic microscope, and the expressions of CD63, CD81, CD90, CD73, CD105, CD29, and CD166 in exosome of mesenchymal stem cells were analyzed by fluorescent activated cellsorting. RESULTS AND CONCLUSION:Mesenchymal stem cells from human umbilical cord blood secreted exosome which exhibited el iptic or saucer-like shape and its diameter ranged from 40 to 100 nm with membrane structure. Exosome could express the common surface adhesion molecules CD63, CD81 and the surface adhesion molecules CD90, CD73, CD105, CD29, CD166 of mesenchymal stem cells. These findings indicate that exosome may be secreted by mesenchymal stem cells of human umbilical cord blood, which contains plasma membrane proteins of umbilical cord blood mesenchymal stem cells.

5.
Article in Chinese | WPRIM | ID: wpr-547743

ABSTRACT

Objective To know the influencing factors for the popularization of tap water in endemic fluorosis areas. Methods The families were randomly selected from Dongdingwang and Xinzhuang in Donghai county,Jiangsu province and were investigated with questionnaire and all members from these families were diagnosed for dental fluorosis with Dean’s method on May 2006. The influencing factors were analyzed with multifactor non-conditional Logistic regression. Results There was no significant difference on the popularization of tap water between the 2 villages (P=0.454). The relative location of the home in village was the risk factor. The size and the economic condition of the families and the householder’s knowledge on the tap water were protective factors. Conclusion The popularization of tap water should be extremely improved based on the improvement of the main influencing factors.

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