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1.
Article in Chinese | WPRIM | ID: wpr-887936

ABSTRACT

Glycyrrhizae Radix et Rhizoma, a traditional Chinese herbal medicine, mainly contains triterpenoids, flavonoids, polysaccharides, coumarins and volatile oils with many pharmacological activities such as anti-tumor, anti-bacterial, anti-viral, anti-inflammatory, immune regulatory and anti-fibrotic effects. The widespread applications of Glycyrrhizae Radix et Rhizoma in food, medicine and chemical industries make its demand increase gradually. Therefore, the quality guarantee of the medicinal is of great value. Starting from the elaboration of chemical components and pharmacological effects of Glycyrrhizae Radix et Rhizoma and the introduction to the concept of quality marker(Q-marker), this study analyzed the Q-markers of Glycyrrhizae Radix et Rhizoma from the aspects of plant phylogene-tics, chemical component specificity, traditional efficacy, traditional medicinal properties, absorbed components, different processing methods and so on, which provides reference for quality evaluation, development and utilization of Glycyrrhizae Radix et Rhizoma.


Subject(s)
Drugs, Chinese Herbal/pharmacology , Glycyrrhiza , Rhizome , Triterpenes
2.
Article in Chinese | WPRIM | ID: wpr-796473

ABSTRACT

Objective@#To explore the genetic basis for a child affected with multiple malformations.@*Methods@#Genomic DNA was extracted from peripheral blood samples from the child and her parents. Tro whole exome sequencing and bioinformatics analysis were carried out. Suspicted mutations were verified by PCR and Sanger sequencing.@*Results@#The patient, a 2-year-old girl, presented with multiple malformations including dysmorphism, skeletal malformations and ambigulous genitalia. Through genetic testing, she was diagnosed with Antley-Bixler syndrome caused by compound heterozygous mutations of the POR gene (c.919G>T and c. 1615G>A), which were derived from her mother and father, respectively.@*Conclusion@#The compound heterozygous mutations of the POR gene probably underlie the Antley-Bixler syndrome in this patient.

3.
Article in Chinese | WPRIM | ID: wpr-776751

ABSTRACT

OBJECTIVE@#To explore the genetic basis for a child affected with multiple malformations.@*METHODS@#Genomic DNA was extracted from peripheral blood samples from the child and her parents. Tro whole exome sequencing and bioinformatics analysis were carried out. Suspicted mutations were verified by PCR and Sanger sequencing.@*RESULTS@#The patient, a 2-year-old girl, presented with multiple malformations including dysmorphism, skeletal malformations and ambigulous genitalia. Through genetic testing, she was diagnosed with Antley-Bixler syndrome caused by compound heterozygous mutations of the POR gene (c.919G>T and c.1615G>A), which were derived from her mother and father, respectively.@*CONCLUSION@#The compound heterozygous mutations of the POR gene probably underlie the Antley-Bixler syndrome in this patient.


Subject(s)
Abnormalities, Multiple , Genetics , Antley-Bixler Syndrome Phenotype , Genetics , Child, Preschool , Cytochrome P-450 Enzyme System , Genetics , Female , Humans , Mutation , Whole Exome Sequencing
4.
Article in Chinese | WPRIM | ID: wpr-850944

ABSTRACT

Objective: The HPLC fingerprints of water extracts and alcohol extracts of Glycyrrhiza uralensis from different producing areas were established in order to provide reference for the quality control and the extraction and processing methods. Methods: The HPLC fingerprints of water extracts and alcohol extracts of 10 batches of G. uralensis from different producing areas were established by HPLC method, and the similarity evaluation and hierarchical cluster analysis of the obtained fingerprint data were carried out. Results: The fingerprint of water extracts and ethanol extracts of G. uralensis was established. There were 16 common peaks were identified from fingerprints of water extracts, and six components were identified, meanwhile, the fingerprints of water extracts showed that the similarity of 10 batches of G. uralensis was in the range of 0.030 and 0.999. Sixty common peaks were identified from fingerprints of ethanol extracts, and seven components were identified, meanwhile, the fingerprints of ethanol extracts showed that the similarity of 10 batches of G. uralensis were all greater than 0.85. The results of hierarchical cluster analysis showed that the water extracts and alcohol extracts of G. uralensis in different areas could be well classified, suggesting that natural factors such as the climate, soil conditions and other natural factors had a significant impact on the internal quality of licorice. Conclusion: A stable and reliable HPLC fingerprint evaluation method for water extracts and alcohol extracts of G uralensis has been established, which can provide a powerful theoretical basis and a guidance for the study of quality standard of G. uralensis and the optimization of extraction and processing technology, and provide a scientific basis for the choice of clinical medication.

5.
Article in English | WPRIM | ID: wpr-690646

ABSTRACT

We sought to identify the differences between adolescents left behind in their home villages/towns (LBA) and non-left behind adolescents (NLB) on subjective well-being and family functioning due to parental migration in south China. We used a stratified cluster sampling method to recruit middle school students in a city experiencing population-emigration in Jiangxi Province in 2010. Participants included adolescents from families with: (1) one migrant parent, (2) both parents who migrated, or (3) non-left behind adolescents (i.e., no migrant parent). To determine predictors of subjective well-being, we used structural equation models. Adolescents left behind by both parents (LBB) were less likely to express life satisfaction (P = 0.038) in terms of their environments (P = 0.011) compared with NLB. A parent or parents who migrated predicts lower subjective well-being of adolescents (P = 0.051) and also lower academic performance. Being apart from their parents may affect family functioning negatively from an adolescent's viewpoint. Given the hundreds of millions of persons in China, many who are parents, migrating for work, there may be mental health challenges in some of the adolescents left behind.


Subject(s)
Adolescent , Aging , China , Emigration and Immigration , Female , Humans , Male , Mental Health , Parent-Child Relations , Parents , Psychology, Adolescent , Rural Population , Socioeconomic Factors , Surveys and Questionnaires , Transients and Migrants
6.
The Journal of Practical Medicine ; (24): 2371-2373, 2014.
Article in Chinese | WPRIM | ID: wpr-455204

ABSTRACT

Objective To discuss the anticancer role of arsenic trioxide (ATO) with cisplatin on human oral carcinoma HSQ-89 cells. Methods The human oral epidermoid HSQ-89 cells were chosen as the subjects. Different concentrations of ATO were added into Cisplatin(DDP)-treated cells. The inhibition rate of tumor cells was detected by MTT assay. Results Different concentrations of ATO (0,2.5,5,7.5,10,12.5 μmol/mL) were added into oral cancer HSQ-89 cells which have been treated with DDP (15 μg/mL). The inhibition rate of tumor cells were 26.9%, 67.5%, 73.0%, 88.5%, 90.4%, 98.7%respectively; The combined application of ATO with cisplatin could improve the inhibition rate of HSQ-89 cells in a dose-dependent relation. Conclusion The combined application of ATO and DDP can produce a synergistic action of inhibition on oral cancer cell.

7.
International Eye Science ; (12): 2147-2150, 2014.
Article in Chinese | WPRIM | ID: wpr-637038

ABSTRACT

AlM: To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells ( HRCECs ) proliferation and vascular endothelial growth factor ( VEGF) expression in high glucose environment. METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3rd ~ 4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose + ( 50ü L/mL, 100ü L/mL, 200ü L/mL ) different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression. RESULTS:MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group (P0. 05). lmmunocytochemical assay showed that:50ü L/mL, 100ü L/mL, 200ü L/mL Coix seed oil acted at HRCECs 48h, the expression of VEGF decreased significantly compared with the high glucose control group ( P CONCLUSlON:Coix seed oil can inhibit the HRCECs proliferation and suppress the VEGF expression in high glucose environment.

8.
Article in Chinese | WPRIM | ID: wpr-328044

ABSTRACT

As a potent anticoagulant, leech a traditional Chinese medicine, has become increasing topics. Hirudin, which is the primary effective component in leech, is a specific and efficient inhibitor of thrombin, mainly used in prevention and treatment of thrombus on the clinic practice. However, there is still no accurate and convenient method reported about the determination of it's biological activity. This paper reported a method for the determination of the biological activity the of extract from hirudo. The extra thrombin, which was not inhibited by hirudin in the extract from hirudo, reacted with N-benzoyl-L-arginine ethyl ester and was determined. The biological activity of the hirudo extract was determined, indirectly. The linear of calibration curve and accuracy were both perfect, the method was accurate and reliable.


Subject(s)
Animals , Arginine , Biological Factors , Pharmacology , Enzyme Assays , Methods , Enzyme Inhibitors , Pharmacology , Hirudins , Pharmacology , Hirudo medicinalis , Chemistry , Thrombin
9.
China Pharmacy ; (12)2005.
Article in Chinese | WPRIM | ID: wpr-533944

ABSTRACT

OBJECTIVE: To establish a method for the determination of the peroxide value of hydroxycamptothecin freeze-dried liposome. METHODS: Colorimetric method with ferric thiocyanate and UV spectrophotometry were adopted for the content determination of peroxide value at detection wavelength of 500 nm. RESULTS: The linear range of iron was 0.2~4 ?g?mL-1 (r=0.999 0) with an average recovery of 99.2% (RSD=1.2%, n=9). The peroxide value of 3 batches of hydroxycamptothecin freeze-dried liposome was 0.48,0.45,0.43 mmol?kg-1. CONCLUSION: The method is sensitive and precise for the determination of the peroxide value of liposome.

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