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1.
Journal of Chinese Physician ; (12): 645-649, 2021.
Article in Chinese | WPRIM | ID: wpr-884099

ABSTRACT

Objective:To analyze the superficial retinal blood flow around the optic disc of optical coherence tomography angiography (OCTA) in non-arteritic anterior ischemic optic neuropathy (NA-AION) patients with affected eyes and contralateral healthy eyes, compared with healthy people.Methods:From May 2018 to may 2019, 30 patients with NA-AION in Xi′an No. 1 Hospital were selected. The changes of superficial retinal blood flow density and perfusion around the optic disc on OCTA of 30 patients with NA-AION, whose contralateral healthy eyes were compared. The pattern visual evoked potential (PVEP) of both eyes was also compared. 30 healthy people with no history of eye disease and history of eye surgery were selected as healthy control group, and differences between healthy control group and contralateral healthy eye group in NA-AION patients of the superficial retinal blood flow density and perfusion around the optic disc were compared.Results:The average values of serum homocysteine, apolipoprotein E and low density lipoprotein in 30 patients with NA-AION were higher than the normal reference range. Compared with the contralateral healthy eyes, the effected eyes of NA-AION patients had lower retinal blood flow density and perfusion in the center, inner layer and complete area of the optic disc, with significantly difference ( P<0.05); there was no significant difference in the latency and amplitude of PVEP-P100 between the two groups ( P>0.05). There was no significant difference in the density and perfusion of superficial retinal blood flow in the center, inner layer and complete area of the optic disc between the contralateral healthy eyes of NA-AION patients and healthy control ( P>0.05). Conclusions:OCTA is a safe, rapid and non-invasive test, and it can detect blood perfusion defects promptly, which can be used as the most commonly test method for patients with NA-AION in order to make auxiliary diagnosis and gain time for clinical treatment.

2.
Article in Chinese | WPRIM | ID: wpr-828321

ABSTRACT

OBJECTIVE@#To determine the type and carrier rate of deafness-related variants in Dongguan, China.@*METHODS@#A total of 16 182 subjects were screened. Heel blood samples were collected from newborns, while peripheral venous blood samples were collected from the remainders. For each individual, 100 variations of 18 deafness susceptibility genes were detected.@*RESULTS@#In total 1631 deafness-related variants (including 5 homozygous mutations) were detected, which gave a detection rate of 10.08%. The detection rate of SLC26A4 gene variants was the highest (845 cases, 5.22%), which was followed by GJB2 (673 cases, 4.16%), GJB3 (100 cases, 0.62%), TMC1 (12 cases, 0.07%), and MYO15A (1 case, 0.01%). The detection rate for GJB2 c.235delC variant was the highest (524 cases, 3.24%), which was followed by SLC26A4 IVS7-2A>G variant (270 cases, 1.67%). Thirty three individuals (0.20%) carried two variants at the same time, 7 of them (0.04%) carried compound heterozygous variants of the same gene.@*CONCLUSION@#To expand the range of screening can help with determination of the carrier status and provision of early intervention and genetic counseling for the examinees.


Subject(s)
China , DNA Mutational Analysis , Deafness , Genetics , Genes , Genetic Counseling , Genetic Predisposition to Disease , Genetic Testing , Genetic Variation , Humans , Infant, Newborn , Mutation , RNA, Ribosomal
3.
International Eye Science ; (12): 1468-1472, 2020.
Article in Chinese | WPRIM | ID: wpr-822983

ABSTRACT

@#AIM:To evaluate the short-term efficacy and safety of intravitreal dexamethasone implant(IDI)for patients with retinal vein occlusion-related macular edema(RVO-ME)by using optical coherence tomography(OCTA).<p>METHODS: Seventeen eyes in 17 patients with RVO-ME were treated with intravitreal injection of sustained-release IDI. The best corrected visual acuity(BCVA), central macular thickness(CMT)and superficial retinal vascular network were observed at baseline, 1d, 1mo, 2mo, 3mo, 4mo and 6mo.<p>RESULTS: CMT was significantly reduced from baseline by 24h after injection and improved further during the 3mo follow-up, and the improvement of visual acuity was consistent with that of CMT. OCTA showed improvement from baseline in terms of decreased number and size of cysts and restoration of the retinal vascular network. A slight increase in intraocular pressure was observed in 2 eyes after injection. There were no systemic side effects associated with injection or medication during follow-up.<p>CONCLUSION: CMT reduced as early as 24h after the injection of IDI, with further reduction during follow-up, and OCTA could visually observe the changes of retinal vascular network in macular area.

4.
International Eye Science ; (12): 200-203, 2019.
Article in Chinese | WPRIM | ID: wpr-712996

ABSTRACT

@#AIM: To investigate the protective mechanism of hydrogen on retinal senescence induced by oxidative stress.<p>METHODS: Mice were randomly divided into three groups: control group, model group and treatment group. Animal models of retinal oxidative stress injury were established by injecting sodium iodate solution into mice caudal vein. Harvesting the mice retina, Western-blot was used to detect the level of proteins related to DNA damage response such as ATM, NF-κB, cyclin D1 and HMGB1 that associated with DNA repair.<p>RESULTS: SA-β-gal staining showed that the blue-green deposits in treatment group were reduced than that in model group. The expression of DNA damage reactive protein in treatment group ATM, cyclin D1, NF-κB(0.10±0.009, 0.32±0.01, 0.19±0.002)were significantly lower than those in the model groups(0.77±0.08, 0.70±0.02, 0.36±0.01), and the differences were statistically significant(all <i>P</i><0.01). At the same time, the expression of DNA repair protein HMGB1 in treatment group(0.927±0.06)were notably higher than that in model group(0.383±0.07)and the difference was statistically significant(<i>P</i><0.01).<p>CONCLUSION: H<sub>2</sub> can attenuate senescence by inhibiting oxidative-stress induced DNA damage.

5.
Article in English | WPRIM | ID: wpr-763136

ABSTRACT

PURPOSE: Although the interferon α (IFNα) signaling and the paired-like homeodomain transcription factor 2 (PITX2) have both been implicated in the progression of breast cancer (BCa), it remains obscure whether these two pathways act in a coordinated manner. We therefore aimed to elucidate the expression and function of PITX2 during the pathogenesis of endocrine resistance in BCa. MATERIALS AND METHODS: PITX2 expression was assessed in BCa tissues using quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunohistochemistry and in experimentally induced letrozole-resistant BCa cells using RT-qPCR and immunoblotting. Effects of PITX2 deregulation on BCa progression was determined by assessing MTT, apoptosis and xenograft model. Finally, using multiple assays, the transcriptional regulation of interferon-inducible transmembrane protein 1 (IFITM1) by PITX2 was studied at both molecular and functional levels. RESULTS: PITX2 expression was induced in letrozole-resistant BCa tissues and cells, and PITX2 induction by IFNα signaling powerfully protected BCa cells against letrozole insult and potentiated letrozole-resistance. Mechanistically, PITX2 enhanced IFNα-induced AKT activation by transactivating the transcription of IFITM1, thus rendering BCa cells unresponsive to letrozoleelicited cell death. Additionally, ablation of IFITM1 expression using siRNA substantially abolished IFNα-elicited AKT phosphorylation, even in the presence of PITX2 overexpression, thus sensitizing BCa cells to letrozole treatment. CONCLUSION: These results demonstrate that constitutive upregulation of PITX2/IFITM1 cascade is an intrinsic adaptive mechanism during the pathogenesis of letrozole-resistance, and modulation of PITX2/IFITM1 level using different genetic and pharmacological means would thus have a novel therapeutic potential against letrozole resistance in BCa.


Subject(s)
Apoptosis , Breast Neoplasms , Breast , Cell Death , Heterografts , Immunoblotting , Immunohistochemistry , Interferons , Phosphorylation , Polymerase Chain Reaction , Reverse Transcription , RNA, Small Interfering , Transcription Factors , Transcriptional Activation , Up-Regulation
6.
International Eye Science ; (12): 1097-1100, 2018.
Article in Chinese | WPRIM | ID: wpr-695382

ABSTRACT

· AIM:To investigate the efficacy and safety of short-term application of pranoprofen eye drops in the treatment of dry eyes in senile patients.· METHODS:Totally 100 elderly patients with dry eyes treated in our hospital from June 2016 to May 2017 were randomly divided into 2 groups.All cases were bilateral onset,and the right eyes were marked as the observation eyes.The 50 eyes in the observation group were treated with 1 g/L pranoprofen eye drops combined with 1 g/L sodium hyaluronate eye drops,while 50 in the control group were treated with 1 g/L sodium hyaluronate eye drops.The results of Schirmer Ⅰ,ocular surface disease index (OSDI),break-up time (BUT),ocular surface staining(OSS),human leukocyte antigen-DR (HLA-DR) and CD11b in conjunctival epithelial cells before and at 2wk after treatment,and adverse reactions in 2 groups were observed.· RESULTS:Before treatment,results of Schirmer I,OSDI,BUT,OSS,HLA-DR and CD11b in the two groups were similar,there were no statistically significant differences (P>0.05).After treatment,OSDI,OSS,HLA-DR,CD11b significantly decreased,BUT significantly increased in both groups,the differences were statistically significant (P< 0.05).After treatment,OSDI,OSS and HLA-DR in the observation group were significantly lower than those in the control group,BUT in the observation group was significantly higher than those in the control group,the differences were statistically significant (P< 0.01).The expression of HLA-DR was positively correlated with OSDI and OSS,and negatively correlated with BUT,and the correlations were statistically significant (P<0.06).· CONCLUSION:Short term application of pranoprofen eye drops can effectively enhance the efficacy in treating elderly patients with dry eyes,release clinical symptoms,and the therapeutic mechanism may be related to the inhibitory effect on HLA-DR.

7.
International Eye Science ; (12): 1843-1846, 2018.
Article in Chinese | WPRIM | ID: wpr-688605

ABSTRACT

@#AIM: To investigate the effect of crystal turbidity on retinal oxygen saturation in patients with diabetic cataract. <p>METHODS:This was a cross-sectional study. Totally 68 patients with 68 eyes of diabetic cataract admitted to our hospital from June 2017 to December 2017 were selected as subjects. Retinal oximetry was used to measure the blood oxygen saturation of the retinal veins, veins, and their supraorbital, nasal, subnasal, and infraorbital branches. The objective scatter index(OSI)of the eye was measured by Visual Quality Analysis System II, and the degree of opacity of the lens was graded according to OSI. <p>RESULTS:The blood oxygen saturation of the retinal artery and its branches in this group were 101.39%±10.84%, 106.19%±11.40%, 103.22%±10.91%, 102.36%±20.31%, and 101.29%±13.88%, respectively. The oxygen saturation of the retinal vein and its branches were 62.51%±8.95%, 66.37%±10.74%, 64.81%±8.97%, 58.37%±13.85%, and 58.66%±19.94%, respectively. The difference between arteriovenous oxygen saturation was 40.72%±12.08%. In this group of patients, 68 patients with 68 eyes had an OSI value of 4.21±3.14. Among them, 15 eyes were turbid at the first stage, 14 eyes were turbid at level 2, 23 eyes were turbid at level 3, and 16 eyes were turbid at level 4. Pearson correlation analysis showed that the retinal veins, veins and their branches were negatively correlated with OSI(both <i>P</i><0.01). The difference in retinal arterial and venous oxygen saturation was positively correlated with OSI(<i>P</i><0.01). There were significant differences in the blood oxygen saturation between the retinal veins, veins and their branches in patients with different degrees of lens opacity(<i>P</i><0.05). The retinal arterial, venous and branch oxygen saturation of patients with opacity of lens 4 was significantly lower than that of patients with opacity of lens 1, 2, and 3, and the difference was statistically significant(<i>P</i><0.05). There were no significant differences in blood oxygen saturation between the patients with opacity of lens 1, 2, and 3(<i>P</i>>0.05). There was no significant difference in the difference of arteriovenous oxygen saturation between the patients with different degrees of lens opacity(<i>P</i>>0.05).<p>CONCLUSION: In patients with diabetic cataract, when the degree of lens opacity is 1 to 3, the degree of abnormality of retinal blood oxygen metabolism is not obvious. When the degree of lens opacity reaches 4, the blood oxygen saturation of the retinal veins, veins and their branches will decrease.

8.
Chinese Pharmacological Bulletin ; (12): 1226-1230, 2018.
Article in Chinese | WPRIM | ID: wpr-705180

ABSTRACT

Aim To explore the effect of rhynchophyl-line on the expression of tyrosine hydroxylase ( TH) in hippocampus of methamphetamine-induced condition place preference ( CPP) mice. Methods Metham- phetamine was injected intraperitoneally to mice, and the expression of TH was observed by immunohisto-chemistry and Western blot. Results The CPP mouse model was established successfully by methamphet-amine ( 4 mg·kg-1) . Ketamine ( 15 mg·kg-1) , rhynchophylline low dosage group (40 mg·kg-1) and rhynchophylline high dosage group ( 80 mg·kg-1) could remove the effect of methamphetamine on CPP mice. The result of immunohistochemistry showed that methamphetamine ( 4 mg·kg-1) could increase the number of TH positive cells in hippocampus while ket-amine (4 mg·kg-1), rhynchophylline (40, 80 mg· kg-1) group could attenuate the change. Western blot-ting indicated the expression of TH of model group in-creased significantly, whereas ketamine ( 15 mg· kg-1) , rhynchophylline ( 40, 80 mg·kg-1) group presented less expression. Conclusions The CPP in-duced by methamphetamine in mice may be inhibited to some extent by rhynchophylline, and its mechanism may be associated with the expression of TH.

9.
Chinese Pharmacological Bulletin ; (12): 756-759, 2018.
Article in Chinese | WPRIM | ID: wpr-705122

ABSTRACT

Exosomes are nanoscale vesicles produced and secre-ted into extracellular fluid by all cells. They mediate cell com-munication through carrying and transferring informational car-goes ( proteins, nucleic acids, lipids and so on ) to recipient cells. In central nervous system, exosomes can be released from all cell types including neurons, neural stem cells and neuroglia cells. These exosomes shuttle nucleic acids ( miRNAs, mRNAs and so on) and play an important role in nervous system devel-opment and function as well as diseases including Alzheimer's disease and drug addiction. Furthermore, the functional effects and targeting characteristics of exosomes-shuttle-RNAs suggest that exosomes-shuttle-RNAs can be diagnostic and therapeutic targets. In this review, we elaborate the effects, functions and mechanisms of exosomes-shuttle-RNAs in order to gain a new recognition of CNS development and diseases.

10.
Neurology Asia ; : 193-202, 2017.
Article in English | WPRIM | ID: wpr-629144

ABSTRACT

Objective: To assess fluid-attenuated inversion recovery (FLAIR) vascular hyper-intensity (FVH) and explore its relationship with CT perfusion (CTP) penumbral/infarct core mismatch ratio and diffusion weighted imaging (DWI) final infarct volume in acute ischemic stroke (AIS) patients with middle cerebral artery occlusion (MCAO). Methods: The CTP and MRI images of 38 AIS patients with MCAO were reviewed. The FVH score (longitudinal direction) [FVH score (L)] and FVH score (transverse direction) [FVH score (T)] were quantified on the FLAIR images. The FVH score (L) (range, 0-16) was based on a rostrocaudal extension of FVH and the FVH score (T) (range, 0-3) was based on FVH supply of the occluded MCA territory. The mismatch ratio was calculated from the ratio of the [mean transit time - cerebral blood volume (CBV)] lesion/CBV lesion on the CTP images. The DWI infarct volume was measured on the DWI images. Results: The mismatch ratio was larger for the group of FVH score (L)=7~8 than those of FVH score (L)=5~6 and FVH score (L)=3~4 (p=0.03), whereas the DWI infarct volume was smaller (p=0.04). Similarly, the mismatch ratio of FVH score (T)=2~3 group was larger than FVH score (T)=1 group (p=0.01), whereas the DWI infarct volume was smaller (p=0.02). Both FVH score (L) and FVH score (T) correlated positively with mismatch ratio (P=0.02, P=0.001, respectively), but negatively with DWI infarct volume (P=0.03, P=0.004, respectively). Conclusions: Higher FVH score is associated with larger mismatch ratio and smaller DWI infarct volume in AIS patients with MCAO. FLAIR vascular hyperintensity may represent collateral arterial circulation, and may play a role in protecting the ischemic penumbra.


Subject(s)
Infarction, Middle Cerebral Artery
11.
International Eye Science ; (12): 1449-1452, 2016.
Article in Chinese | WPRIM | ID: wpr-637901

ABSTRACT

?AIM:To investigate the effects of Bevacizumab on the proliferation and the expression of E -Cadherin and fibronectin in human retinal pigment epithelial cell ( ARPE-19) in vitro.?METHODS: Different concentrations (0, 0.625, 1.25, 2.5, 5.0mg/mL) of bevacizumab were exposed to ARPE-19 cells, then cell viability was analyzed by CCK-8, cell cycle was determined by flow cytometry, and the expression of E-Cadherin and fibornectin was detected by Western blot and RT-PCR.?RESULTS:The concentration as 2.5mg/mL or 5.0mg/mL of bevacizumab was shown to effectively suppress the proliferation and cell cycle of ARPE-19 cell (P<0.05). In addition, 2.5mg/mL or 5.0mg/mL of bevacizumab could downregulate the expression of E-cadherin and promote the transcription of fibronection gene (P<0.05).?CONCLUSION:High concentration of bevacizumab was able to inhibit ARPE-19 proliferation, downregulate E-Cadherin expression and promote fibronectin expression, indicating epithelial-mesenchymal transition induced by bevacizumab in ARPE-19 cell.

12.
Chinese Pharmacological Bulletin ; (12): 1352-1355, 2015.
Article in Chinese | WPRIM | ID: wpr-478092

ABSTRACT

Amphetamine-type stimulants ( ATS ) , a group of new-type synthetic drugs mainly in psychological dependence, are abused more and more severely in recent years. MicroRNAs ( MiRNAs ) are an important class of endogenous non-coding small RNAs that mediate posttranscriptional negatively regulation of gene expression by targeting specific mRNA sequences to in-hibit the translation of mRNAs or degrade the expression of mR-NAs. ATS can induce the changes in the expression of miRNAs in addiction-related brain regions which directly involve in the regulation of ATS-induced addictive behaviors. Therefore, to study the regulatory role of miRNAs in ATS-induced addiction has important implications for dependent mechanisms of new-type drugs and the discovery of the new targets of drug actions.

13.
Chinese Pharmaceutical Journal ; (24): 1111-1116, 2015.
Article in Chinese | WPRIM | ID: wpr-859523

ABSTRACT

OBJECTIVE: To investigate the effect and mechanism of allomatrine in proliferation and invasion in vitro inhibition of human lung cancer A549 cell line. METHODS: After treatment with allomatrine, MTS assay was employed to determine the proliferation of cancer cells, flow cytometry to determine the apoptosis rate, cell cycle distribution and intracellular ROS production, transwell assay to determine the cell invasion potential in vitro, adhesion assay to determine the cell adhesion potential in vitro, realtime PCR and Western blotting assay to determine the expression of apoptosis related gene Survivin, Bcl-2 and caspase-3/8, the cell cycle related protein CDK-2, adhesion molecule CD44, matrix metalloproteinases MMP-2/9 and the phosphorylation of AKT, report gene assay to determine the transcription activity of NF-KB and the activity of ubiquitin proteasome. RESULTS: Allomatrine significantly inhibited the proliferation and invasion in vitro of A549 cells, flow cytometry showed that apoptosis rate and ROS production was increased and the cell cycle was halted by G2/M phase, the expression of pro-apoptosis gene caspase-3/8 was upregulated while the anti-apoptosis proteins Survivin and Bcl-2 was downregulated, the expression of CDK-2, CD44 and MMP-2/9 was downregulated, and the phosphorylation of AKT was downregulated, the transcription activity of NF-KB and the activity of ubiquitin proteasome were inhibited after Allomatrine treatment. CONCLUSIONS: Allomatrine was able to inhibit proliferation and invasion in vitro of human lung cancer A549 cell line by inducing ROS production, promoting apoptosis, arresting cell cycle, inhibiting ubiquitin proteasome and regulating tumor related gene expression.

14.
Article in Chinese | WPRIM | ID: wpr-854387

ABSTRACT

Objective: To develop an UPLC-Q-TOF-MS method for the determination of seven components (naringin, hesperidin, strychnine, brucine, agrimol B, wogonin, and protocatechuic acid) in Pingxiao Tablets. Methods: The analysis was performed on a Waters Acquity BEH C18 column (100 mm×2.1 mm, 1.7 μm) with the mixture of acetonitrile-water-formic acid as mobile phase, and the flow rate was 0.45 mL/min; MS scanning mode: negativion; Sample volume: 2.0 μL. Results: The linear relationship between the concentration and peak areas of the seven compounds were all linear (r>0.9996). The average recoveries (n=6) were between 99%-101%. Conclusion: This method is simple, accurate, and reliable to determine the seven contents in Pingxiao Tablets for the quality control.

15.
Article in Chinese | WPRIM | ID: wpr-600706

ABSTRACT

Objective To understand healthcare-associated urinary tract infection (HA-UTI)and pathogens causing HA-UTI in intensive care unit (ICU)patients,so as to provide scientific basis for the prevention and control of HA-UTI. Methods Targeted surveillance data about HA-UTI in 32 hospitals in 2013 were analyzed.Results A total of 23 680 ICU patients were monitored,157 cases of HA-UTI occurred,HA-UTI rate was 0.66%;the usage rate of urinary tract cathe-ter was 80.83%,catheter-associated UTI was 1.25‰.A total of 162 pathogenic strains were detected,the percentage of fungi,gram-negative bacteria,and gram-positive bacteria was 40.74% (n=66);31.48 % (n=51),and 27.78% (n=45)respectively.Conclusion The main pathogens causing HAI-URI are fungi,comprehensive intervention measures should be taken to control HA-UTI in ICU patients.

16.
Neurology Asia ; : 15-21, 2015.
Article in English | WPRIM | ID: wpr-628389

ABSTRACT

The ABCD2 score is validated for evaluating short-term stroke risk after transient ischemic attack (TIA); however, whether it is able to predict the long-term risk of vascular outcome remains uncertain. Recently a new tissue-based definition of TIA has been proposed. The ABCD2 scores of 145 TIA patients admitted to our hospital were retrospectively calculated and stratified into two categories: ≤ 3 points (low risk); 4-7 points (moderate-high risk). At a median follow-up of 81 months, new vascular events were recorded. Follow-up data were available in 107 patients. Seventy one patients had a moderate-high ABCD2 score. Sixty six patients experienced a cerebral ischemic event; 8 a myocardial infarction; 7 died of cerebrovascular or cardiovascular cause. Moderate-high ABCD2 score was significantly associated with the further cerebral ischemic events (hazard ratio [HR], 1.755; 95% confidence interval [CI], 1.019 to 3.024) and with the combined endpoint (HR, 1.818; 95% CI, 1.079 to 3.063). Our study shows that the ABCD2 score may also be used to predict long-term vascular outcome after tissue-based definition of TIA. Moderate-high ABCD2 score is associated with an increased general vascular risk in the long-term follow-up after TIA.


Subject(s)
Stroke
17.
Acta Pharmaceutica Sinica ; (12): 1326-1330, 2014.
Article in Chinese | WPRIM | ID: wpr-299131

ABSTRACT

In order to solve the problem of selection and in vivo delivery problem in siRNA treatment, hepatitis B virus (HBV) HBx gene which could be targeted by siRNA was studied. The siRNA expression plasmid which specific inhibits HBx expression was obtained by in vitro selection via a dual-luciferase plasmid including HBx-Fluc fusion protein expression domain. The selected siRNA expression plasmid was then encapsulated in PEG-modified cationic liposome, which was devoted into pharmacodynamic studies at both cellular and animal level. The results illustrated that the cationic liposome which encapsulated siRNA expression plasmid could effectively inhibit HBx gene expression both in vitro and in vivo.


Subject(s)
Cations , Gene Expression Regulation, Viral , Hepatitis B virus , Genetics , Liposomes , Chemistry , Plasmids , RNA, Small Interfering , Chemistry , Trans-Activators , Genetics , Metabolism
18.
Acta Pharmaceutica Sinica ; (12): 256-259, 2014.
Article in Chinese | WPRIM | ID: wpr-297983

ABSTRACT

To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.


Subject(s)
Animals , Antineoplastic Agents , Blood , Pharmacokinetics , Chromatography, High Pressure Liquid , Immunoglobulin M , Blood , Liposomes , Blood , Pharmacokinetics , Male , Metabolic Clearance Rate , Mitoxantrone , Blood , Pharmacokinetics , Polyethylene Glycols , Chemistry , Pharmacokinetics , Rats , Rats, Wistar , Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry
19.
Acta Pharmaceutica Sinica ; (12): 256-9, 2014.
Article in Chinese | WPRIM | ID: wpr-448730

ABSTRACT

To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.

20.
Article in Chinese | WPRIM | ID: wpr-459823

ABSTRACT

This article was aimed to study anti-inflammatory effects of petroleum ether extract from Citrullus lanatus vine (PEECLV) and explore the mechanism. The methods of ear swelling with xylene, increasing of peritoneal capil-lary permeability, and paw edema with egg albumin were used in the establishment of mice models, respectively. Granuloma with cotton pellet, and paw edema with carrageenan were used in the establishment of rat models, re-spectively. Observation was made on anti-inflammatory effects of PEECLV. The content of nitrous oxide (NO) and nitric oxide synthase (NOS), as well as the level of TNF-α and IL-1β in blood serum were measured among granu-loma with cotton pellet rat models. Contents of malondialdehyde (MDA), prostaglandin E2 (PGE2), 5-HT, histamine (His), and protein in inflammatory exudates from the paw edema with carrageenan rat model, as well as contents of NO and NOS in blood serum, and activity of superoxide dismutase (SOD) were measured in order to study the possi-ble anti-inflammatory effects. The results showed that PEECLV can suppress the ear swelling with xylene, the in-creasing of peritoneal capillary permeability, and the paw edema with egg albumin in mice, as well as granuloma with cotton pellet, paw edema with carrageenan in rats. It had anti-inflammatory effects at different degrees. The content of NO and NOS, as well as the level of TNF-α and IL-1β in blood serum were reduced in granuloma with cotton pellet rat models. The content of MDA, PGE2, 5-HT, His, and protein content were reduced in inflammatory exudates from the paw edema with carrageenan rat model. The increasing of NO and NOS in blood serum was inhib-ited. And the activity of SOD was increased. It was concluded that PEECLV had certain anti-inflammatory effects. Its mechanism may be related to the influence of the production of inflammatory mediators and antioxidant.

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