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1.
China Pharmacy ; (12): 313-318, 2022.
Article in Chinese | WPRIM | ID: wpr-913089

ABSTRACT

OBJECTIVE To study different extraction fractions of Agrimonia pilosa against h epatic fibrosis. METHODS Using hepatic stellate cells HSC-T 6 of rats as objects ,the effects of different extraction fractions (total extract ,ethyl acetate fraction , petroleum ether fraction and n-butanol fraction )with different concentrations (0.5,5,50,500,5 000 μg/mL,calculated by raw drug)of A. pilosa on the proliferation of HSC-T 6 cells were detected (after treated for 24,48,72 h);median inhibition concentration(IC50)was also caculated. Platelet-derived growth factor (PDGF-BB)was used to induce the activation of HSC-T 6 cells to establish hepatic fibrosis cell model. Flow cytometry was used to detect the effects of different extraction fractions of A. pilosa on apoptosis of HSC-T 6 cells. The expression of collagen Ⅰ(Col-Ⅰ)in the supernatant was detected by enzyme linked immunosorbent assay. The expressions of α-smooth muscle actin (α-SMA),Col-Ⅰ,B-cell lymphoma- 2(Bcl-2),Bcl-2-associated X protein (Bax)and caspase- 3 were detected by Western blot assay. RESULTS Total extract ,ethyl acetate fraction ,petroleum ether fraction and n-butanol fraction of A. pilosa could significantly increase the apoptotic rate of HSC-T 6 cells(P<0.01). After treated for 24 h,IC50 of above fractions were 50.17,20.75,5.82,4.09 μg/mL,respectively. After intervened with PDGF-BB ,the expression of Col- Ⅰ in supernatant of HSC-T 6 cells as well as protein expression of Col- Ⅰ,α-SMA,Bcl-2,Bax and caspase- 3 in HSC-T6 cells were increased significantly (P<0.01). After intervened with different extraction fractions of A. pilosa ,most of the expressions of above proteins in HSC-T 6 cell culture supernatant or cells were significantly reversed compared with PDGF-BB group (P<0.05 or P<0.01), and the intervention effect of n-butanol fraction of A. pilosa was the most significant. CONCLUSIONS Different extraction fractions of A. pilosa can inhibite the proliferation of HSC-T 6 cells and induce their apoptosis;n-butanol fraction from A. pilosa may be an effective fraction to exert the effect of anti-hepatic fibrosis.

2.
Chinese Journal of General Surgery ; (12): 896-902, 2022.
Article in Chinese | WPRIM | ID: wpr-994530

ABSTRACT

Objective:To investigate the clinical outcomes of patients with intrahepatic cholangiocarcinoma (ICC) undergoing surgical resection.Methods:Patients who undergoing radical surgical resection for ICC from Jan 2015 to Apr 2021 at the Department of General Surgery, the First Affiliated Hospital of Anhui Medical University were included in this retrospective cohort study.Results:There were 67 patients in the final analysis, The median follow-up duration was 14 months (range: 1-60 months). Firty three patients (79.1%) had tumor recurrence, 52 patients (77.6%) died, Among them, 49 patients (73.1%) died from tumor recurrence. The 1-、2-、and 3-year accumulated disease-free and overall survival rate were 35.6%, 19.6%, 16.8% and 53.7%, 32.4%, 20.8%. respectively. The overall survival rate of the group without microvascular invasion was significantly better than those of the group with microvascular invasion ( χ2=5.916, P=0.015). CA19-9≥1 000 U/ml was the only independent risk factor for the disease-free survival. CA19-9≥1 000 U/ml、blood loss≥600 ml、microvascular invasion and tumor recurrence were the independent risk factors for the overall survival. Conclusion:For ICC patients with single tumor, when the tumor diameter is less than 5 cm and has no microvascular invasion, surgical resection is recommended, and a satisfactory prognosis could be achieved.

3.
Protein & Cell ; (12): 417-432, 2020.
Article in English | WPRIM | ID: wpr-828761

ABSTRACT

Vision formation is classically based on projections from retinal ganglion cells (RGC) to the lateral geniculate nucleus (LGN) and the primary visual cortex (V1). Neurons in the mouse V1 are tuned to light stimuli. Although the cellular information of the retina and the LGN has been widely studied, the transcriptome profiles of single light-stimulated neuron in V1 remain unknown. In our study, in vivo calcium imaging and whole-cell electrophysiological patch-clamp recording were utilized to identify 53 individual cells from layer 2/3 of V1 as light-sensitive (LS) or non-light-sensitive (NS) by single-cell light-evoked calcium evaluation and action potential spiking. The contents of each cell after functional tests were aspirated in vivo through a patch-clamp pipette for mRNA sequencing. Moreover, the three-dimensional (3-D) morphological characterizations of the neurons were reconstructed in a live mouse after the whole-cell recordings. Our sequencing results indicated that V1 neurons with a high expression of genes related to transmission regulation, such as Rtn4r and Rgs7, and genes involved in membrane transport, such as Na/K ATPase and NMDA-type glutamatergic receptors, preferentially responded to light stimulation. Furthermore, an antagonist that blocks Rtn4r signals could inactivate the neuronal responses to light stimulation in live mice. In conclusion, our findings of the vivo-seq analysis indicate the key role of the strength of synaptic transmission possesses neurons in V1 of light sensory.

4.
Protein & Cell ; (12): 417-432, 2020.
Article in English | WPRIM | ID: wpr-828597

ABSTRACT

Vision formation is classically based on projections from retinal ganglion cells (RGC) to the lateral geniculate nucleus (LGN) and the primary visual cortex (V1). Neurons in the mouse V1 are tuned to light stimuli. Although the cellular information of the retina and the LGN has been widely studied, the transcriptome profiles of single light-stimulated neuron in V1 remain unknown. In our study, in vivo calcium imaging and whole-cell electrophysiological patch-clamp recording were utilized to identify 53 individual cells from layer 2/3 of V1 as light-sensitive (LS) or non-light-sensitive (NS) by single-cell light-evoked calcium evaluation and action potential spiking. The contents of each cell after functional tests were aspirated in vivo through a patch-clamp pipette for mRNA sequencing. Moreover, the three-dimensional (3-D) morphological characterizations of the neurons were reconstructed in a live mouse after the whole-cell recordings. Our sequencing results indicated that V1 neurons with a high expression of genes related to transmission regulation, such as Rtn4r and Rgs7, and genes involved in membrane transport, such as Na/K ATPase and NMDA-type glutamatergic receptors, preferentially responded to light stimulation. Furthermore, an antagonist that blocks Rtn4r signals could inactivate the neuronal responses to light stimulation in live mice. In conclusion, our findings of the vivo-seq analysis indicate the key role of the strength of synaptic transmission possesses neurons in V1 of light sensory.

5.
China Pharmacy ; (12): 878-882, 2019.
Article in Chinese | WPRIM | ID: wpr-817006

ABSTRACT

OBJECTIVE: To investigate the effects of adiponectin (APN) on the expression of myocardial AMPK in myocardial insulin resistance (IR) model dogs during cardiopulmonary bypass (CPB). METHODS: Totally 24 dogs were randomly divided into control group, model group, APN group (36 μg/kg), AMPK inhibition group (APN 36 μg/kg+AMPK inhibitor compound C 0.5 mg/kg), with 6 dogs in each group. All dogs underwent CPB; except for control group without medicine, CPB myocardial IR model were established in other groups, and perfused with St.Thomas cardiac cardioplegia lipid no medicine or containing relevant drugs after main artery block. Coronary sinus blood and carotid artery blood samples were collected before bypass and after 15, 90 min reperfusion following 60 min myocardial ischemia. Left ventricular apical tissue was taken, and the uptake rate of myocardial glucose and insulin resistance index (IRI) were determined and calculated; the changes of myocardial injury indexes (cTnT concentration) and cardiac function indexes (LVSP, +dp/dtmax) were monitored. The level of p-AMPK was detected. RESULTS: There was no statistical significance in above indexes of dogs before bypass (P>0.05). Compared with control group, the rate of myocardial glucose uptake, the levels of LVSP, +dp/dtmax and p-AMPK in model group were decreased significantly after 15, 90 min reperfusion (P<0.05), and the concentrations of IRI and cTnT were increased significantly (P<0.05). Compared with model group, the rate of myocardial glucose uptake, LVSP, +dp/dtmax and p-AMPK were increased significantly in APN group and AMPK inhibitor group (P<0.05), while the concentrations of IRI and cTnT were decreased significantly (P<0.05); moreover, the effect of APN group was better than that of AMPK inhibitor group (P<0.05). CONCLUSIONS: APN can promote myocardial glucose uptake and metabolism, and contribute the recovery of cardiac function, the mechanism of which may be associated with increasing the activity of AMPK.

6.
Chinese Journal of Tissue Engineering Research ; (53): 1455-1460, 2019.
Article in Chinese | WPRIM | ID: wpr-743866

ABSTRACT

BACKGROUND: Myocardial ischemia/reperfusion injury is one of the most common complications in ischemic cardiomyopathy and open heart surgery. The development of bone marrow mesenchymal stem cells provides a new method for clinical prevention and treatment of myocardial ischemia/reperfusion injury. OBJECTIVE: To review the therapeutic effect and potential mechanisms of bone marrow mesenchymal stem cells in the treatment of myocardial ischemia/reperfusion injury, in order to provide a theoretical basis for the clinical application of bone marrow mesenchymal stem cells. METHODS: Chinese Journal Full-text Database (CNKI) , WanFang, and PubMed were retrieved for articles related to the use of bone marrow mesenchymal stem cells for myocardial ischemia-reperfusion injury published from January 2000 to October 2018. The search terms were "bone marrow mesenchymal stem cells; myocardial ischaemia/reperfusion; research process" in Chinese and "bone marrow mesenchymal stem cells; myocardial ischaemia/reperfusion; cell therapy; clinical trial studies" in English. Old and repetitive viewpoints were excluded, the searched literatures were sorted out, and finally 56 articles were included for further analysis and discussion. RESULTS AND CONCLUSION: (1) In this paper, we summarize paracrine factors, exosomes miRNA and their effects in the treatment of myocardial ischemia/reperfusion injury with bone marrow mesenchymal stem cells, such as anti-inflammation, anti-apoptosis, anti-fibrosis, repair of myocardium and neovascularization. (2) We also summarize the possible molecular mechanisms of bone marrow mesenchymal stem cells involved in the treatment of myocardial ischemia/reperfusion injury, such as the role of mitochondrial fusion protein 2, regulation of myocardial autophagy, and regulation of AMPK/mTOR signaling pathway. Overall, we attempt to provide a theoretical basis for the clinical application of bone marrow mesenchymal stem cells in the treatment of myocardial ischemia/reperfusion injury.

7.
Chinese Journal of Tissue Engineering Research ; (53): 1455-1460, 2019.
Article in Chinese | WPRIM | ID: wpr-743814

ABSTRACT

BACKGROUND: Myocardial ischemia/reperfusion injury is one of the most common complications in ischemic cardiomyopathy and open heart surgery. The development of bone marrow mesenchymal stem cells provides a new method for clinical prevention and treatment of myocardial ischemia/reperfusion injury. OBJECTIVE: To review the therapeutic effect and potential mechanisms of bone marrow mesenchymal stem cells in the treatment of myocardial ischemia/reperfusion injury, in order to provide a theoretical basis for the clinical application of bone marrow mesenchymal stem cells. METHODS: Chinese Journal Full-text Database (CNKI), WanFang, and PubMed were retrieved for articles related to the use of bone marrow mesenchymal stem cells for myocardial ischemia-reperfusion injury published from January 2000 to October 2018. The search terms were "bone marrow mesenchymal stem cells; myocardial ischaemia/reperfusion; research process" in Chinese and "bone marrow mesenchymal stem cells; myocardial ischaemia/reperfusion; cell therapy; clinical trial studies" in English. Old and repetitive viewpoints were excluded, the searched literatures were sorted out, and finally 56 articles were included for further analysis and discussion. RESULTS AND CONCLUSION: (1) In this paper, we summarize paracrine factors, exosomes miRNA and their effects in the treatment of myocardial ischemia/reperfusion injury with bone marrow mesenchymal stem cells, such as anti-inflammation, anti-apoptosis, anti-fibrosis, repair of myocardium and neovascularization. (2) We also summarize the possible molecular mechanisms of bone marrow mesenchymal stem cells involved in the treatment of myocardial ischemia/reperfusion injury, such as the role of mitochondrial fusion protein 2, regulation of myocardial autophagy, and regulation of AMPK/mTOR signaling pathway. Overall, we attempt to provide a theoretical basis for the clinical application of bone marrow mesenchymal stem cells in the treatment of myocardial ischemia/reperfusion injury.

8.
Chinese Journal of Postgraduates of Medicine ; (36): 131-134, 2017.
Article in Chinese | WPRIM | ID: wpr-508482

ABSTRACT

Objective To explore the effect of infrared spectral analysis in analyzing of the chemical composition of renal staghorn calculi and its relationship with urinary tract infections. Methods From June 2014 to June 2016, the clinical data of 186 patients with renal staghorn calculi were collected. The stone composition were analyzed by infrared spectroscopy and traditional chemical titration, and the stones infection were detected by microbial analysis system. The relation between stones infection, urinary tract infection and stone composition were analyzed. Results The results of infrared spectroscopy and traditional chemical titration in detecting renal staghorn calculi ingredient had no significant differences (P>0.05). In 186 patients, 56 patients (30.11%) was in infected group, and 130 patients (69.89%)was in non-infected group. The abnormal urine rate, urinary tract infection rate, medistream urine positive infection rate and cotton swabs positive infection rate in infected group were was significantly higher than those in non-infected group: 73.21%(41/56) vs. 50.77%(66/130), 19.64%(11/56) vs. 3.85%(5/130), 50.00%(28/56) vs. 6.15%(8/130), 67.86%(38/56) vs. 8.46%(11/130), P<0.01. The carbonate apatite stones rate and six water magnesium ammonium phosphate rate in infected group were significantly higher than those in non-infected group: 21.43%(12/56) vs. 5.37%(7/130), 57.14%(32/56) vs. 2.31%(3/130), P<0.01. The calcium oxalate rate and uric acid rate in non-infected group were significantly higher than those in infected group:50.00%(65/130) vs. 5.36%(3/56), 24.62%(32/130) vs. 1.79%(1/56), P<0.01. Conclusions Analysis of staghorn calculi ingredient caused by urinary bacterial infection with infrared spectroscopy is simple, reliable and easy to operate. It is important for postoperative infection prevention.

9.
Chinese Journal of Biochemical Pharmaceutics ; (6): 357-359, 2017.
Article in Chinese | WPRIM | ID: wpr-620543

ABSTRACT

Objective To investigate the MRI findings of recurrent supurative colangitis complicated by intrahepatic cholangiocarcinoma and clinical diagnostic value.Methods 264 patients with recurrent suppurative cholangitis who were admitted into the hospital during June 2010 to July 2014 were selected as the research objects.All of the patients were given magnetic resonance imaging(MRI)and surgical pathological examination or biopsy.The Resultsof surgical pathological examination certified that there 43 patients(52 lesions)complicated by intrahepatic cholangiocarcinoma.The results of pathological examination were taken as the golden standard to evaluate the accuracy, specificity and sensitivity of MRI in the clinical diagnosis of recurrent supurative colangitis complicated by intrahepatic cholangiocarcinoma.The MRI findings were analuzed.ResultsAmong the 264 patients with recurrent supurative cholangitis in this group, there were 43 patients(52 lesions)with complicated intrahepatic cholangiocarcinoma confirmed by surgical pathological examination or biopsy, accounting for 16.3% of total number of subjects;MRI results showed that 218 cases were true negative, 41 cases true positive, 2 cases false negative and 3 cases false positive.The diagnostic sensitivity was 95.3%(41/43), specificity 98.6%(218/221)and the accuracy 98.1%(259/264).Conclusion MRI in the diagnosis of recurrent supurative cholangitis complicated by intrahepatic cholangiocarcinoma is of higher accuracy, sensitivity and specificity.MRI findings are obvious.It is worthy of clinical promotion.

10.
Chinese Journal of Biochemical Pharmaceutics ; (6): 114-116, 2017.
Article in Chinese | WPRIM | ID: wpr-612912

ABSTRACT

Objective To study treatment effect of qingre shugan tuihuang tang in the treatment of newly diagnosed hepatitis B with jaundice.Methods88 patients newly diagnosed hepatitis B with jaundice in our hospital from August 2015 to December 2016 were randomly divided into control group and treatment group according to different modes of treatment, control group was given protecting liver and reducing enzyme, antivirus and other conventional therapy, treatment group added qingre shugan tuihuang tang on the basis of control group, changes of liver function indexes, changes of bilirubin, changes of clinical syndromes and treatment effect in the two groups were compared.ResultsSerum total bilirubin (TBIL), direct bilirubin (DBIL), Alanine transaminase (ALT) and prothrombin time (PT) in the two groups were significantly decreased (P<0.05), albumin was significantly increased (P<0.05), and rangeability of TBIL, DBIL, ALT and albumin in treatment group after the treatment were significantly higher than control group, the difference was statistically significant (P<0.05);hepatitis B virus DNA copy numbers in the two groups were significantly decreased (P<0.05), treatment group was significantly lower than control group, the difference was statistically significant (P<0.05);yellow tint of sclera and skin, gastric distention, rib-side pain, anorexia, drowsiness and fatigue symptom scores in the two groups were significantly decreased (P<0.05), decrease of treatment group was significantly higher than control group, the difference was statistically significant (P<0.05);total effective rate of treatment group was 97.73%, while the control group was 81.82%, the two groups had significant difference (P<0.05).ConclusionQingre shugan tuihuang tang in the treatment of newly diagnosed hepatitis B with jaundice can improve symptoms of jaundice significantly, improve liver function injury, effect is remarkable, its clinical application is very important.

11.
Chinese Journal of cardiovascular Rehabilitation Medicine ; (6): 497-500, 2014.
Article in Chinese | WPRIM | ID: wpr-473773

ABSTRACT

Objective:To explore the correlation among serum uric acid (UA) level and severity of coronary heart disease (CHD) ,adverse cardiovascular events .Methods :Clinical data of 403 patients undergoing coronary angiog-raphy in our hospital were retrospectively analyzed .They were divided into CHD group [n=308 ,including 137 cases with stable angina pectoris (SAP) and 171 cases with acute coronary syndrome (ACS)] and normal control group (n=95 ,non-CHD patients) .UA level was compared among all groups and its correlation with CHD severity was fur-ther analyzed .According to UA level ,CHD patients were divided into low UA group (n=147) and high UA group (n=161) ,after 12-month follow-up ,incidence rate of major adverse cardiovascular events (MACE) uring follow-up was compared between two groups .Results:Serum UA level of ACS group was significantly higher than that of nor-mal control group [ (318.44 ± 69.07)μmol/L vs .(295.38 ± 80.08)μmol/L ,P=0.003];along with number of dis-eased coronary vessels increased and CHD severity aggravated (Gensini score rose ) ,serum UA level showed an in-creasing trend [single-branch (316.58 ± 95.27 ) μmol/L vs . double-branch (335.26 ± 43.26 ) μmol/L vs . multi-branch (346.53 ± 86.74) μmol/L ;low score group (312.42 ± 48.26) μmol/L vs .middle score group (346.58 ± 47.36)μmol/L vs .high score group (363.84 ± 54.68)μmol/L , P<0.05 or <0.01] .Pearson correlation analysis indicated that serum UA level was positively correlated with Gensini score (r=0.583 ,P<0.05);MACE incidence rate in high UA group was significantly higher than that of low UA group (58.38% vs .36.24% , P=0.012) .Con-clusion:Serum uric acid level is positively correlated with severity of coronary heart disease ,which may be an inde-pendent risk factor for coronary heart disease .

12.
Chinese Journal of Infectious Diseases ; (12): 321-325, 2009.
Article in Chinese | WPRIM | ID: wpr-394241

ABSTRACT

Objective To study a functional variable fragment of heavy chain(VH)antibody against the terminal protein(TP)region of hepatitis B virus(HBV)polymerase introduced by human immunodeficiency virus Tat protein transduction domain(TAT)and the inhibitive activity of TAT-VH on the replication of HBV in vitro.Methods The gene encoding TAT-VH was cloned into prokaryotic expression vector pET28a(+).Recombinant plasmid was transduced into E coli BL21(DE3)LysS,then the protein was expressed and purified.The purified TAT-VH fusion protein was added into HepG2.2.15 cell culture.The transduction efficiency was evaluated by indirect fluorescence assay(IFA).The cytotoxicity of TAT-VH was detected by Methabenzthiazuron(MTT)assay.HBV DNA level in HepG2.2.15 cell culture was measured using quantitative polymerase chain reaction(PCR).The data were analyzed by one-factor analysis of variance and t test.Results TAT-VH fusion protein was successfully expressed and purified.It was confirmed by IFA and MTT assay that TAT-VH was introduced into HepG2.2.15 cells and the cell growth was not affected.The level of HBV DNA in supernatant of HeDG2.2.15 cell culture with 5 000 nmol/L TAT-VH was(1.211±0.132)lg copy/mL,which was significantly lower than control group[(5.325±0.041)lg copy/mL,t=72.91,P<0.05].Meanwhile,the level of intracellular HBV DNA was(3.521±0.411)lg copy/mL,which was significantly lower than control group[(8.532±0.132)lg copy/mL.t=28.41,P<0.05].Conclusion The HBV replication is inhibited by anti-TP TAT-VH antibodies in vitro,which provides valuable experimemal basis for developing therapy of HBV infection with intracellular antibody.

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