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1.
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 280-286, 2023.
Article in Chinese | WPRIM | ID: wpr-986028

ABSTRACT

Objective: To investigate the wearing of masks and the knowledge of masks among high-risk positions for overseas import and pollution transmission. Methods: From May 14 to 17, 2022, a convenient sampling method was used to conduct an online survey among 963 workers in high-risk positions for overseas import and pollution transmission in Beijing. The behaviors of individual use and wearing masks, the distribution and supervision of the unit, the knowledge of personal mask protection and the subjective feelings of wearing masks were analyzed. The χ(2) test and logistic regression model were used to analyze the influencing factors of the correct selection of masks. Results: The majority of the workers in high-risk positions for overseas import and pollution transmission were male (86.0%, 828/963), age concentration in 18-44 years old (68.2%, 657/963), and the majority of them had college or bachelor degrees (49.4%, 476/963). 79.4%(765/963) of the workers chose the right type of masks, female, 45-59 years old and high school education or above were the risk factors for correct selection of masks (P <0.05). Workers had good behaviors such as wearing/removing masks, but only 10.5% (101/963) could correctly rank the protective effect of different masks. 98.4% (948/963) of the workers believed that their work units had provided masks to their employees, and 99.1% (954/963) and 98.2%(946/963) of them had organized training and supervision on the use of masks, respectively. 47.4%(456/963) of the workers were uncomfortable while wearing masks. Conclusion: The overall selection and use of masks among occupational groups in high-risk positions for overseas import and pollution transmission in China need to be further standardized. It is necessary to strengthen supervision and inspection on the use of masks among occupational groups, and take improvement measures to improve the comfort of wearing masks.


Subject(s)
Humans , Male , Female , Adolescent , Young Adult , Adult , Middle Aged , Cross-Sectional Studies , Masks , China , Surveys and Questionnaires , Beijing
2.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 730-744, 2023.
Article in English | WPRIM | ID: wpr-1010986

ABSTRACT

Traditionally, Tripterygium hypoglaucum (Levl.) Hutch (THH) are widely used in Chinese folk to treat rheumatoid arthritis (RA). This study aimed to investigate whether the anti-RA effect of THH is related with the gut microbiota. The main components of prepared THH extract were identified by HPLC-MS. C57BL/6 mice with adjuvant-induced arthritis (AIA) were treated with THH extract by gavage for one month. THH extract significantly alleviated swollen ankle, joint cavity exudation, and articular cartilage destruction in AIA mice. The mRNA and protein levels of inflammatory mediators in muscles and plasma indicated that THH extract attenuated inflammatory responses in the joint by blocking TLR4/MyD88/MAPK signaling pathways. THH extract remarkably restored the dysbiosis of the gut microbiota in AIA mice, featuring the increases of Bifidobacterium, Akkermansia, and Lactobacillus and the decreases of Butyricimonas, Parabacteroides, and Anaeroplasma. Furthermore, the altered bacteria were closely correlated with physiological indices and drove metabolic changes of the intestinal microbiota. In addition, antibiotic-induced pseudo germ-free mice were employed to verify the role of the intestinal flora. Strikingly, THH treatment failed to ameliorate the arthritis symptoms and signaling pathways in pseudo germ-free mice, which validates the indispensable role of the intestinal flora. For the first time, we demonstrated that THH extract protects joint inflammation by manipulating the intestinal flora and regulating the TLR4/MyD88/MAPK signaling pathway. Therefore, THH extract may serve as a microbial modulator to recover RA in clincial practice.ver RA in clincial practice.


Subject(s)
Mice , Animals , Gastrointestinal Microbiome , Tripterygium , Myeloid Differentiation Factor 88/genetics , Toll-Like Receptor 4/genetics , Mice, Inbred C57BL , Arthritis, Experimental/drug therapy
3.
Chinese Journal of Radiological Health ; (6): 266-272, 2022.
Article in Chinese | WPRIM | ID: wpr-973402

ABSTRACT

Objective To provide scientific data for the study of tumor prevalence among medical X-ray workers. Methods In a cohort study, radiation exposure and tumor occurrence were collected from medical X-ray workers and normal persons (control) in Chongqing, China. SPSS 24 software was used to calculate the incidence density of tumor in the follow-up cohort, as well as the relative risk (RR) and 95% confidence interval of various malignant tumors. Results A total of 934 subjects were followed up. By the end of the investigation, 109 cases of malignant tumor were recorded. Compared with the control group, the RRs of liver cancer and colorectal cancer were both 3.4. Dose-specific RR was calculated for the worker groups. Compared with the two groups with < 80 mGy and 80~200 mGy cumulative doses, the group with > 200 mGy cumulative dose showed 2.05 and 2.1 RRs for solid cancer, and 1.89 and 2.17 RRs for whole cancer, respectively. Conclusion The risk of liver cancer and colorectal cancer is higher for medical X-ray workers in Chongqing, and the risk of solid cancer and whole cancer is higher in the high cumulative dose group. Therefore, radiation workers should pay attention to the optimization of protection.

4.
Chinese Journal of Biochemistry and Molecular Biology ; (12): 990-998, 2022.
Article in Chinese | WPRIM | ID: wpr-1015773

ABSTRACT

O⁃GlcNAcylation is an O⁃linked⁃β⁃N⁃acetylglucosamine modification attached to the hydroxyl group of serine or threonine residue within the nuclear or cytoplasmic proteins. O⁃GlcNAcylation profoundly influences important biological events, including kinase activity, transcription and translation, and protein degradation. However, there are few summarized reviews on how O⁃GlcNAcylation modulates signaling pathways associated with inflammatory responses. Due to the attachment and removal of the sugar group catalyzed by O⁃GlcNAc transferase and O⁃GlcNAcase, O⁃GlcNAcylation cycles rapidly with a short half⁃life time (within minutes). Therefore, O⁃GlcNAcylation plays a crucial role in various signaling pathways via intricate cross⁃talking with other post⁃translational modifications of protein, such as phosphorylation, acetylation, ubiquitylation, and methylation. Currently, most researchers focused on the Toll⁃like receptor (TLR)⁃initiated NF⁃κB signaling when it comes to the relationship between O⁃GlcNAcylation and inflammation. Evidence has shown that O⁃GlcNAcylation at T352 or at T305 on p65 promotes its nuclear translocation activity, while O⁃GlcNAcylation at S536 blocks the activation of p65 by competing with phosphorylation. Meanwhile, O⁃GlcNAcylation modulates upstream and downstream regulators of NF⁃κB and then governs the polarization of M1/ M2 macrophage and the progress of inflammation reactions. Furthermore, O⁃GlcNAcylation indirectly participates in the kinase activation of MAPKs by interfering with the proteins at the upper reaches (i. e. MEK2 and Ras proteins). Besides, O⁃GlcNAcylation has a profound influence on multiple kinases of PI3K/ AKT signaling. Nevertheless, O⁃GlcNAcylation manipulates inflammation⁃associated transcriptional factors on the JAK/ STAT pathway. Comparatively, the involved signaling transduction for the inflammatory response in vivo is far more complicated and multidimensional than that in vitro. And O⁃GlcNAcylation is widely involved with the onset and development of inflammatory diseases located at the pancreas, liver, lung, gut, and adipose tissues. Novel research has firstly found that gut bacteria expressing O⁃GlcNAcase⁃like hydrolases exert potent prevention on mouse colitis induced by different chemical drugs, which indicates the mediating role of O⁃GlcNAcylation in mutual interactions between gut microbiota and host inflammation. In summary, recent findings provided a novel strategy for preventing and treating inflammatory diseases by targeting O⁃GlcNAcylation.

5.
Chinese Journal of Pharmacology and Toxicology ; (6): 595-600, 2020.
Article in Chinese | WPRIM | ID: wpr-857511

ABSTRACT

OBJECTIVE: To study the distribution, excretion and other pharmacokinetic characteristics of dual target ligand-based lidamycin (DTLL) in rats. METHODS: [125l] radioactivity isotope tracing method was used to determine the pharmacokinetic parameters of DTLL which was injected intravenously via the tail vein of healthy Wistar rats at a single dose of [125l] DTLL 2625 kBq · 0.05 mg-1· kg-1. The samples from rat plasma, tissue, feces, urine and bile were obtained to analyze the distributions of DTLL in rats after administration and to calculate the cumulative excretion scores by using a liquid scintillation counting analyzer with the WinNolin program. RESULTS: The area under the concentration-time curve (AUC0-∞) of DTLL was (184±33) h·μg·L-1, with half time (t1/2) and cleanrance (Cl) at (3.98±0.75) h and (278±41) mL·h-1· kg-1, respectively. DTLL was mainly distributed in whole body tissues without any accumulation observed. The excretion rate of DTLL was (82.5±2.6)% for urine, compared with (10.4±6.6)% in feces and (92.9±7.4)% in total. CONCLUSION: DTLL is distributed rapidly in the whole body without obvious accumulation. Most of the DTLL administered is excreted through feces and urine.

6.
China Journal of Orthopaedics and Traumatology ; (12): 1022-1026, 2020.
Article in Chinese | WPRIM | ID: wpr-879345

ABSTRACT

Periprosthetic infection after hip replacement is a clinical catastrophic disease, which often leads to the failure of the prosthesis. It needs the combination of systemic antibiotics to cure the infection, which brings huge burden to doctors and patients. There are strict indications for debridement and one-stage revision of the prosthesis, and few cases meet the requirements. The second revision is still the gold standard for the treatment of periprosthetic infection. It is suitable for all infection conditions and has a high success rate. On the second phase of renovation, the antibiotic sustained release system plays a key role, and the carrier of antibiotic sustained-release system is the focus of current research, including classic bone cement and absorbable biomaterials. Bone cement has strong mechanical strength, but the antibiotic release shows a sharp decline trend; the absorbable biomaterials can continuously release antibiotics with high concentration, but the mechanical strength is poor, so it could not use alone. The combination of bone cement and absorbable biomaterials will be an ideal antibiotic carrier. PMMA is the most commonly used antibiotic carrier, but the antibiotic release concentration is decreased sharply after 24 hours. It will be difficult to control the infection and increase the risk of bacterial resistance if it is lower than the minimum inhibitory concentration. The biodegradable materials can release antibiotics completely, with long release time and high concentration, but low mechanical strength. Antibiotic spacer plays an important role in the control of infection. In the future, how to further extend the antibiotic release time of antibiotic sustained-release system, increase the amount of antibiotic release and maintain the mechanical strength of the material will be studied.


Subject(s)
Humans , Anti-Bacterial Agents/therapeutic use , Arthroplasty, Replacement, Hip/adverse effects , Bone Cements , Hip Prosthesis , Prosthesis-Related Infections/surgery , Reoperation
7.
Chinese Journal of Contemporary Pediatrics ; (12): 317-322, 2019.
Article in Chinese | WPRIM | ID: wpr-774079

ABSTRACT

OBJECTIVE@#To investigate the causes and clinical features of children with traumatic brain injury (TBI) who need hospitalization or emergency observation.@*METHODS@#A retrospective analysis was performed for the clinical data of 126 children with TBI who were admitted to the emergency department from January 1, 2014 to August 31, 2016, including causes of injury and clinical features.@*RESULTS@#Of the 126 children, there were 95 boys and 31 girls, with a mean age of 2.8 years (range 0.8-5.5 years). The children aged <1 year accounted for 38.1% (48/126), and 26 children died. The two most common types of TBI were epidural hematoma (54.0%) and subarachnoid hemorrhage (50.8%). Of the 126 children, 83 (65.9%) had a Glasgow Coma Scale score of ≤8 within 24 hours after admission. There were different causes of TBI and places where TBI occurred in different age groups. The two leading causes of TBI were falls (51.6%) and road traffic injuries (42.9%). Compared with those in the other age groups, the children in the age <1 year group were most likely to experience injury due to falls (46%; P=0.023). Thirty-five percent of all TBI due to road traffic injuries occurred in the children aged 3-6 years (P<0.001). Most TBI cases occurred at home (47.6%) or on roads/streets (45.2%). Among those who experienced TBI at home, the children aged <1 year accounted for the highest proportion of 48% (P=0.002), and 53% of the patients aged 3-6 years experienced TBI on roads/streets. The most common cause of death in children with TBI was road traffic injury, which accounted for 69%. Among those who died, the children aged <1 year accounted for the highest proportion (62%).@*CONCLUSIONS@#There are different causes of TBI and places where TBI occurs in different age groups. Among children with TBI, the children aged <1 year account for the highest proportion and have the highest number of deaths, with falls at home as the most common cause of TBI. Children aged 3-6 years tend to suffer TBI due to road traffic injury. Road traffic injury is the leading cause of death.


Subject(s)
Child , Child, Preschool , Female , Humans , Male , Brain Injuries, Traumatic , Glasgow Coma Scale , Hospitalization , Retrospective Studies
8.
Chinese Journal of Hematology ; (12): 889-894, 2019.
Article in Chinese | WPRIM | ID: wpr-1012091

ABSTRACT

Objective: To investigate the current status and real performance of the detection of RUNX1-RUNX1T1 fusion transcript levels and WT1 transcript levels in China through interlaboratory comparison. Methods: Peking University People's Hospital (PKUPH) prepared the samples for comparison. That is, the fresh RUNX1-RUNX1T1 positive (+) bone morrow nucleated cells were serially diluted with RUNX1-RUNX1T1 negative (-) nucleated cells from different patients. Totally 23 sets with 14 different samples per set were prepared. TRIzol reagent was added in each tube and thoroughly mixed with cells for homogenization. Each laboratory simultaneously tested RUNX1-RUNX1T1 and WT1 transcript levels of one set of samples by real-time quantitative PCR method. All transcript levels were reported as the percentage of RUNX1-RUNX1T1 or WT1 transcript copies/ABL copies. Spearman correlation coefficient between the reported transcript levels of each participated laboratory and those of PKUPH was calculated. Results: ①RUNX1-RUNX1T1 comparison: 9 samples were (+) and 5 were (-) , the false negative and positive rates of the 20 participated laboratories were 0 (0/180) and 5% (5/100) , respectively. The reported transcript levels of all 9 positive samples were different among laboratories. The median reported transcript levels of 9 positive samples were from 0.060% to 176.7%, which covered 3.5-log. The ratios of each sample's highest to the lowest reported transcript levels were from 5.5 to 12.3 (one result which obviously deviated from other laboratories' results was not included) , 85% (17/20) of the laboratories had correlation coefficient ≥0.98. ②WT1 comparison: The median reported transcript levels of all 14 samples were from 0.17% to 67.6%, which covered 2.6-log. The ratios of each sample's highest to the lowest reported transcript levels were from 5.3-13.7, 62% (13/21) of the laboratories had correlation coefficient ≥0.98. ③ The relative relationship of the reported RUNX1-RUNX1T1 transcript levels between the participants and PKUPH was not always consistent with that of WT1 transcript levels. Both RUNX1-RUNX1T1 and WT1 transcript levels from 2 and 7 laboratories were individually lower than and higher than those of PKUPH, whereas for the rest 11 laboratories, one transcript level was higher than and the other was lower than that of PKUPH. Conclusion: The reported RUNX1-RUNX1T1 and WT1 transcript levels were different among laboratories for the same sample. Most of the participated laboratories reported highly consistent result with that of PKUPH. The relationship between laboratories of the different transcript levels may not be the same.


Subject(s)
Humans , China , Core Binding Factor Alpha 2 Subunit , Leukemia, Myeloid, Acute , RUNX1 Translocation Partner 1 Protein , Real-Time Polymerase Chain Reaction , Transcription, Genetic , WT1 Proteins
9.
Chinese Journal of Hematology ; (12): 40-45, 2019.
Article in Chinese | WPRIM | ID: wpr-1011924

ABSTRACT

Objective: To analyze the percentage of myeloperoxidase (MPO)-positive acute myeloid leukemia (AML) blast cells, and to explore the correlation of MPO expression with the clinical features, gene alterations, therapeutic response and prognosis of AML. Methods: The expressions of MPO in BM blasts cells of 233 newly diagnosed AML were retrospectived analyzed, they were divided into two groups using the percentage of MPO-positive blast [low (≤70%) and high (>70%)], clinical features, gene alterations, chemotherapy efficacy and prognosis were compared between the two groups. Results: ①Of the 233 patients, 121(51.9%) were in the low MPO group, and the rest 112(48.1%) in the high MPO group. Favorable-risk group according NCCN guidelines of AML was always MPO-high (χ(2)=32.773, P<0.001), while MPO-low was closely related to poor-risk (χ(2)=7.078, P=0.008); ②DNMT3A mutation (χ(2)=6.905, P=0.009), spliceosome genes mutation (SF3B1/SRSF2/U2AF1) (χ(2)=5.246, P=0.022), RUNX1 mutation (χ(2)=4.577, P=0.032), ASXL1 mutation (χ(2)=7.951, P=0.005) and TP53 mutation (P=0.004) were more likely to be seen in the low MPO group, while C-KIT mutation (χ(2)=8.936, P=0.003) and CEBPA mutation (χ(2)=12.340, P<0.001) were more frequent in the high MPO group, especially CEBPA double mutation; ③The rates of first complete remission in the low MPO group were significantly lower than that in the high MPO group (38.8% vs 68.1%, χ(2)=15.197, P<0.001). Multivariate analysis showed that low MPO positivity significantly affected the CR(1) unfavourably. ④The overall survival (OS) and the progression-free survival (PFS) were significantly worse in the low MPO group (18.0% vs 89.4% for OS, and 11.5% vs 56.7% for PFS, P<0.001). Multivariate analysis disclosed that the low number of MPO was significantly unfavourable prognostic factor. ⑤The low MPO group still showed a worse survival even when restricted to the patients with normal karyotype, the OS and the PFS were 31.1% and 18.8% respectively. Conclusions: AML with different MPO expression percentage had a unique gene mutation spectrum. Low expression of MPO was an independent risk factor for CR(1), OS and PFS in AML patients, which may be a simple and highly significant factor for AML patients when evaluating the therapeutic efficacy and prognosis.


Subject(s)
Humans , Leukemia, Myeloid, Acute , Mutation , Peroxidase , Prognosis , Remission Induction
10.
West China Journal of Stomatology ; (6): 133-139, 2018.
Article in Chinese | WPRIM | ID: wpr-688049

ABSTRACT

<p><b>OBJECTIVE</b>To explore the regulatory mechanism of inducible nitric oxide synthase (NOS-2) expression related to proliferation of Tca8113 cells.</p><p><b>METHODS</b>RNAi mediated by short hairpin RNAs was utilized to knock down NOS-2, protein kinase C (PKC)-α, PKC-β and PKC-δ. Griess Reagent played a significant role on the detection of NO product after NOS-2 silence. The cell proliferation was determined by CCK8 method. Quantitative real-time polymerase chain reaction (q-PCR) was recruited to check the mRNA level of NOS-2, PKC-α, PKC-β and PKC-δ after treated by a variety of ways. Eventually, the measure of phosphorylation of extracellular regulated protein kinases (ERK)1/2 was performed by Western blotting in PMA-treated Tca8113 cells.</p><p><b>RESULTS</b>The cell viability of Tca8113 decreased obviously after transfected with NOS-2 siRNA (P<0.01). PKC reduced the expression level of NOS-2 mRNA (P<0.05). PKC-α, PKC-β and PKC-δ worked together to regulate the level of NOS-2 mRNA (P<0.01). Motigen-activated protein kinase kinase (MEK)/ERK signaling pathway regulated the level of NOS-2 mRNA negatively (P<0.05). PKC down regulated the level of NOS-2 mRNA through MEK/ERK signaling pathway (P<0.05).</p><p><b>CONCLUSIONS</b>PKC regulates the mRNA level of NOS-2 related to proliferation through MEK/ERK signaling pathway in Tca8113 cells.</p>

11.
Braz. j. med. biol. res ; 51(7): e7220, 2018. tab, graf
Article in English | LILACS | ID: biblio-889115

ABSTRACT

An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induced cytotoxicity in HepG2.2.15 cells, which was accompanied by the cleavage of caspase-3 protein and down-regulation of Bcl-2/Bax ratio. Moreover, urolithin A suppressed the protein expressions of Sp-1, Lin28a, and Zcchc11, and elevated the expression of microRNA let-7a. Importantly, urolithin A also regulated the Lin28a/let-7a axis in transient HBx-transfected HCC HepG2 cells. Furthermore, urolithin A decelerated the HepG2.2.15 cell invasion, which was involved in suppressing the let-7a downstream factors HMGA2 and K-ras. These findings indicated that urolithin A exerted the antiproliferative effect by regulating the Lin28a/let-7a axis and may be a potential supplement for HBV-infected HCC therapy.


Subject(s)
Humans , RNA-Binding Proteins/drug effects , Carcinoma, Hepatocellular/drug therapy , Coumarins/pharmacology , MicroRNAs/drug effects , Liver Neoplasms/drug therapy , Reference Values , Sincalide/analysis , Time Factors , Virus Replication/drug effects , Cell Survival/drug effects , Blotting, Western , Reproducibility of Results , Analysis of Variance , RNA-Binding Proteins/analysis , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/virology , MicroRNAs/analysis , Cell Proliferation/drug effects , Hep G2 Cells , Real-Time Polymerase Chain Reaction , Liver Neoplasms/genetics , Liver Neoplasms/virology
12.
Biomedical and Environmental Sciences ; (12): 829-833, 2017.
Article in English | WPRIM | ID: wpr-311342

ABSTRACT

Population-based cancer registration data were collected to estimate the cancer incidence and mortality in Wuwei, Hexi Corridor Region, China in 2018. We used the 2011-2013 data to predict the number of new cases and deaths in 2018 and the 2003-2013 data to analyze trends in cancer incidence and mortality. The goal is to enable cancer prevention and control directions. Our results indicated that stomach cancer is the most common cancer. For all cancers combined, the incidence and mortality rates showed significantly increasing trends (+2.63% per year; P < 0.05 and +1.9% per year; P < 0.05). This study revealed a significant cancer burden among the population of this area. Cancer screening and prevention should be performed after an epidemiological study of the cause of the cancer is completed.


Subject(s)
Female , Humans , Male , China , Incidence , Neoplasms , Classification , Epidemiology , Mortality , Population Surveillance , Registries , Rural Population , Urban Population
13.
Biomolecules & Therapeutics ; : 186-193, 2017.
Article in English | WPRIM | ID: wpr-32625

ABSTRACT

Tetrandrine (Tet), a bisbenzylisoquinoline alkaloid, has been reported to have a radiosensitization effect on tumors. However, its effects on human glioma and the specific molecular mechanisms of these effects remain unknown. In this study, we demonstrated that Tet has a radiosensitization effect on human glioma cells. It has been hypothesized that Tet has a radiosensitization effect on glioma cells by affecting the glioma cell cycle and DNA repair mechanism and that ERK mediates these activities. Therefore, we conducted detailed analyses of the effects of Tet on the cell cycle by performing flow cytometric analysis and on DNA repair by detecting the expression of phosphorylated H2AX by immunofluorescence. We used western blot analysis to investigate the role of ERK in the effect of Tet on the cell cycle and DNA repair. The results revealed that Tet exerts its radiosensitization effect on glioma cells by inhibiting proliferation and decreasing the expression of phosphorylated ERK and its downstream proteins. In summary, our data indicate that ERK is involved in Tet-induced radiosensitization of glioma cells via inhibition of glioma cell proliferation or of the cell cycle at G0/G1 phase.


Subject(s)
Humans , Blotting, Western , Cell Cycle , Cell Proliferation , DNA Repair , Fluorescent Antibody Technique , Glioma , Phosphorylation
14.
Chinese Medical Journal ; (24): 636-644, 2016.
Article in English | WPRIM | ID: wpr-328180

ABSTRACT

<p><b>BACKGROUND</b>Population-based cancer registry collects the data on cancer incidence and mortality deaths from covered population to describe and survey the epidemics in certain areas. The aim of this study was to estimate the cancer incidence and mortality in Wuwei, Gansu province, Northwestern China from 2003 to 2012. The goal is to better understand cancer distribution and long-term development of cancer prevention and treatment in Wuwei.</p><p><b>METHODS</b>Data were collected from the Wuwei Cancer Registry between 2003 and 2012. In this registry, data from 46 cancer report centers were included in this analysis. Incidence/mortality rates, age-specific incidence/mortality rates, age-standardized incidence/mortality rates, and cumulative incidence/mortality rates were calculated. Totally, 9,836,740 person-years (5,110,342 for males and 4,726,398 for females) had been monitored over this time period. The gender ratio of male/female was 1.08:1. The number of new cancer cases and related deaths was 24,705 and 17,287 from 2003 to 2012, respectively.</p><p><b>RESULTS</b>The proportion of morphological verification was 74.43%. The incidence of cases identified through death certification only was 1.21%, and the mortality to incidence ratio was 0.70. The average crude incidence was 251.15/100,000 persons (310.61 and 186.87 for males and females per 100,000 persons, respectively). The age-standardized rates by Chinese standard population (ASR-China) and by world standard population (ASR-world) were 207.76 and 245.42 per 100,000 persons, respectively. The crude cancer mortality was 175.74/100,000 persons (228.34 and 118.86 for males and females per 100,000 persons). ASR for China and the world was 149.57 and 175.13/100,000 persons, respectively. The most common cancers and leading causes of cancer-related deaths in Wuwei were as follows: cancers of stomach, esophagus, liver, lung, colorectum, breast, cervix, lymphoma, blood (leukemia), brain, and central nervous system. In Wuwei, during 2003 and 2012, cancer incidence and mortality rates increased by 1.32% and 1.31%/year, respectively. During this time, colorectum cancer incidence and mortality rates increased by 2.69% and 7.54%/year, respectively, in Wuwei. The incidence and mortality of other gastric, esophageal, liver, and lung cancers also all increased.</p><p><b>CONCLUSIONS</b>The results of this study report a more accurate cancer burden among the population of Wuwei, China. Active research of cancers etiology and effective prevention should be established to reduce the incidence and mortality associated with cancers.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Middle Aged , China , Epidemiology , Incidence , Neoplasms , Epidemiology , Mortality , Registries , Retrospective Studies , Time Factors
15.
Acta Pharmaceutica Sinica B ; (6): 222-228, 2016.
Article in English | WPRIM | ID: wpr-309964

ABSTRACT

Taking 12-N-p-chlorobenzyl sophoridinol 2 as a lead, a series of novel sophoridinic derivatives with various 3'-substituents at the 11-side chain were synthesized and evaluated for their anticancer activity from sophoridine (1), a natural antitumor medicine. Among them, the sophoridinic ketones 5a-b, alkenes 7a-b and sophoridinic amines 14a-b displayed reasonable antiproliferative activity with IC50 values ranging from 3.8 to 5.4 μmol/L. Especially, compounds 5a and 7b exhibited an equipotency in both adriamycin (AMD)-susceptible and resistant MCF-7 breast carcinoma cells, indicating a different mechanism from AMD. The primary mechanism of action of 5a was to arrest the cell cycle at the G0/G1 phase, consistent with that of parent compound 1. Thus, we consider 12-chlorobenzyl sophoridinic derivatives with a tricyclic scaffold to be a new class of promising antitumor agents with an advantage of inhibiting drug-resistant cancer cells.

16.
Biomedical and Environmental Sciences ; (12): 24-40, 2016.
Article in English | WPRIM | ID: wpr-258855

ABSTRACT

<p><b>OBJECTIVE</b>To study the nutrition habits among Chinese-Korean children and adolescents in Yanbian Autonomous Prefecture, Jilin, China.</p><p><b>METHODS</b>Data were obtained from the Chinese National Survey on Students' Constitution and Health in 1995, 2000, 2005, and 2010 for Chinese-Korean children and adolescents aged 7-18 years. The number of the subjects included was 4789, 4704, 5875, and 5315, respectively.</p><p><b>RESULTS</b>The rate of the occurrence of stunting showed a declining trend from 1995 to 2010 (for boys: urban, 6.3%; rural, 12.7% in 1995 and 3.5% for both in 2010. For girls: urban, 7.8%; rural, 13.4% in 1995 and 4.2% and 5.5%, respectively, in 2010). Although the ratio of wasting did not show significant differences between the urban and rural children and adolescents in 1995, 2000, 2005, and 2010 respectively, the ratio of occurrence of overweight or obesity increased (for boys: urban, 7.3% and 1.3% in 1995, 17.6% and 12.9% in 2010; rural, 7.0% and 1.3% in 1995, 14.6% and 12.8% in 2010, respectively. For girls: urban, 8.1% and 1.0% in 1995, 17.3% and 8.6% in 2010; rural 5.7% and 0.7% in 1995, 16.4% and 7.4% in 2010, respectively).</p><p><b>CONCLUSION</b>The ratio of malnutrition in children and adolescents in Chinese-Korean areas declined from 1995 to 2010, and the distinction in malnutrition between the urban and rural areas was negligible in 2010. Further, the ratio of overweight and obesity increased over this period.</p>


Subject(s)
Adolescent , Child , Female , Humans , Male , Adolescent Nutritional Physiological Phenomena , Asian People , Child Nutritional Physiological Phenomena , China , Epidemiology , Ethnology , Growth Disorders , Epidemiology , Nutritional Status , Overweight , Epidemiology
17.
Biomedical and Environmental Sciences ; (12): 429-436, 2015.
Article in English | WPRIM | ID: wpr-264566

ABSTRACT

<p><b>OBJECTIVE</b>To estimate the relationship between migration and HIV risky behavior when controlling for gender, age, and educational levels and to evaluate the gender differences in migration, HIV knowledge, and HIV risky behaviors among rural youth in China.</p><p><b>METHODS</b>A cross-sectional, anonymous, investigative questionnaire for 1710 unmarried, out-of-school rural youth, aged between 15 and 24 years, was handed out in Gongzhuling county of Jilin province, China.</p><p><b>RESULTS</b>58.5% of participants had a history of migration, irrespective of gender. There were gender differences observed in other factors such as drug abuse (4.3% for males and 5.5% for females, P<0.01), multiple sexual partners (24.1% for males and 44.1% for females, P<0.01), and HIV knowledge rate (35.2% for males and 25.5% for females, P<0.001). While controlling for gender, age, and educational levels, the relationships between migration and drug abuse, selling sex, and non usage of condoms during last instance of sexual activity were found to be significant. The cases of premarital sex and multiple sexual partners were both not found to be related to migration.</p><p><b>CONCLUSION</b>Among rural youth, the HIV risky behavior such as drug abuse, selling sex, and lack of condom use, is significantly related to migration, while premarital sex and multiple sexual partners seem unrelated to migration.</p>


Subject(s)
Adolescent , Female , Humans , Male , Young Adult , China , Epidemiology , Condoms , HIV Infections , Epidemiology , Health Knowledge, Attitudes, Practice , Human Migration , Risk-Taking , Rural Population , Sex Factors , Sex Work , Substance-Related Disorders , Epidemiology , Surveys and Questionnaires , Transients and Migrants , Psychology , Unsafe Sex , Psychology
18.
Biomedical and Environmental Sciences ; (12): 573-581, 2014.
Article in English | WPRIM | ID: wpr-270562

ABSTRACT

<p><b>OBJECTIVE</b>To analyze and assess secular change in stature in rural children and adolescents in China from 1985 to 2010.</p><p><b>METHODS</b>Data were obtained from the 6 rounds of the Chinese National Survey on Student's Constitution and Health. The subjects enrolled in the study were children and adolescents aged 7-18 years in rural areas of provincial capitals.</p><p><b>RESULTS</b>An overall positive secular trend in stature occurred in rural areas of provincial capitals in China from 1985 to 2010. The overall average increase rates were 3.1 and 2.4 cm/decade for boys and girls, respectively. The total body height increases for grown up boys and girls were 3.6 and 2.3 cm and the increase rates were 1.4 and 0.9 cm/decade, respectively. There were differences in body height increase among eastern, central and western regions. The average body height of the children or adolescents in eastern region was highest, followed by central region and western region. The overall increase rates in central region were highest among the three regions. The difference between eastern/central region and western region was obvious.</p><p><b>CONCLUSION</b>Positive secular trend in stature of children or adolescents has occurred in rural area of China, and rural boys and girls showed a great potential for continuous growth. More attention should be paid to the differences in children's body height between western region and eastern/central region.</p>


Subject(s)
Adolescent , Child , Female , Humans , Male , Asian People , Body Height , Physiology , Child Development , China , Rural Population
19.
Biomedical and Environmental Sciences ; (12): 614-626, 2014.
Article in English | WPRIM | ID: wpr-270559

ABSTRACT

<p><b>OBJECTIVE</b>To assess the prevalence of malnutrition among children and adolescents in Xizang (Tibet).</p><p><b>METHODS</b>We analyzed data from the Chinese National Survey on Students' Constitution and Health for the years 1995, 2000, 2005, and 2010 pertaining to Tibetan children and adolescents in lase (Lhasa), aged 7-18 years old. Numbers of survey subjects for these years were: 2393, 2754, 2397, and 2643, respectively.</p><p><b>RESULTS</b>Our results indicated that the rate of occurrence of stunting in Tibet has evidenced a gradual decline: for boys, from 26.8% in 2000 to 9.3% in 2010; and for girls, from 25.8% in 2000 to 10.8% in 2010. In general, the wasting rate for both boys and girls in Tibet has gradually decreased over time: for boys, from 17.7% in 1995 to 4.6% in 2005; and for girls from 12.5% in 1995 to 2.3% in 2005. The stunting rates of boys aged 7-13 years old and of girls aged 7-11 years old were 67.5% and 53.1%, respectively, while these rates for boys aged 14-18 years old and girls aged 12-18 years old were 32.5% and 46.9%, respectively.</p><p><b>CONCLUSION</b>Stunting and wasting rates of Tibetan children and adolescents indicate a gradual declining trend over time. The stunting rates of both boys and girls during early puberty were significantly higher than those during late puberty.</p>


Subject(s)
Adolescent , Child , Female , Humans , Male , Cross-Sectional Studies , Growth Disorders , Epidemiology , Malnutrition , Epidemiology , Nutritional Status , Physiology , Prevalence , Thinness , Epidemiology , Tibet , Epidemiology
20.
Biomedical and Environmental Sciences ; (12): 794-806, 2014.
Article in English | WPRIM | ID: wpr-270538

ABSTRACT

<p><b>OBJECTIVE</b>Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China.</p><p><b>METHODS</b>Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes (matK, rbcL, and ITS) were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment (PWG) distance, and Tree-Building were tested for discrimination power.</p><p><b>RESULTS</b>The primer universality of all the three markers was high. Except in the case of ITS for Hemerocallis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method.</p><p><b>CONCLUSION</b>DNA barcoding technique provides us a fast tool for clinical identification of poisonous plants in China. We suggest matK, rbcL, ITS used in combination as DNA barcodes for authentication of poisonous plants.</p>


Subject(s)
China , DNA Barcoding, Taxonomic , Reference Standards , DNA Primers , Genetics , DNA, Intergenic , Genetics , Plant Proteins , Genetics , Plants, Toxic , Classification , Genetics , Ribulose-Bisphosphate Carboxylase , Genetics , Sequence Analysis, DNA , Species Specificity
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