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1.
Article in Chinese | WPRIM | ID: wpr-931236

ABSTRACT

Chromium is a harmful contaminant showing mutagenicity and carcinogenicity.Therefore,detection of chromium requires the development of low-cost and high-sensitivity sensors.Herein,blue-fluorescent carbon quantum dots were synthesized by one-step hydrothermal method from alkali-soluble Poria cocos polysaccharide,which is green source,cheap and easy to obtain,and has no pharmacological ac-tivity due to low water solubility.These carbon quantum dots exhibit good fluorescence stability,water solubility,anti-interference and low cytotoxicity,and can be specifically combined with the detection of Cr(Ⅵ)to form a non-fluorescent complex that causes fluorescence quenching,so they can be used as a label-free nanosensor.High-sensitivity detection of Cr(Ⅵ)was achieved through internal filtering and static quenching effects.The fluorescence quenching degree of carbon dots fluorescent probe showed a good linear relationship with Cr(Ⅵ)concentration in the range of 1-100 μM.The linear equation was F0/F=0.9942+0.01472[Cr(Ⅵ)](R2=0.9922),and the detection limit can be as low as 0.25 μM(S/N=3),which has been successfully applied to Cr(Ⅵ)detection in actual water samples herein.

2.
Clinical Medicine of China ; (12): 1192-1195, 2011.
Article in Chinese | WPRIM | ID: wpr-422803

ABSTRACT

Objective To investigate the efficacy,safety and practicability of endoscope-controlled microneurosurgery operations for hypertension ventricular hemorrhage.Methods The efficacy and complications of three operation methods,including endoscope-controlled operation,Burr-hole craniotomy,ventricle puncture and drainage of urokinase infusion,were compared retrospectively.Results The complications of endoscopecontrolled operation was significantly lower than the other two methods(x2 =9.966,P < 0.05).Among the 32patients treated by endoscope-controlled operation,2 patients died after the surgery with a fatality rate of 6.25%.Six months after the surgery,the ADL score estimation showed grade Ⅰ 2 cases,grade Ⅱ 14 cases; grade Ⅲ 11cases ; grade Ⅳ3 cases; grade V2 cases.Compared to the other two groups,there was significant difference(x2 =10.499,P < 0.05).Conclusion Endoscope-controlled operation is an effective and safe method in treating patients with hypertension ventricular hemorrhage with less brain damage,better hemorrhage clearance,and less complications when compared with small bone window craniotomy and ventricle puncture and drainage of urokinase infusion operation methods.

3.
Chinese Journal of Surgery ; (12): 360-362, 2002.
Article in Chinese | WPRIM | ID: wpr-314880

ABSTRACT

<p><b>OBJECTIVE</b>To probe into the incidence, mechanism and clinical characteristics of chronic subdural hematoma (CSDH) evolving from traumatic subdural effusion (TSE).</p><p><b>METHODS</b>The clinical materials of 32 patients with CSDH evolving from TSH were analyzed retrospectively and the correlative literature was reviewed.</p><p><b>RESULTS</b>16.7% of the patients with TSH evolved into CSDH. The time of evolution was 22 - 100 days after head injury. All patients were cured with hematoma drainage.</p><p><b>CONCLUSIONS</b>TSE is one of the origins of CSDH. The clinical characteristics of TSE evolving into CSDH include polarization of patient age, and chronic small effusion. The patients are usually injured deceleratedly and accompanied with mild cerebral damage.</p>


Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Male , Middle Aged , Chronic Disease , Hematoma, Subdural , Retrospective Studies , Subdural Effusion , Wounds and Injuries
4.
Article in Chinese | WPRIM | ID: wpr-540867

ABSTRACT

Objective To construct genetically engineered cells that secrete human TH and GDNF at the same time by stable co-transfection pcDNA3.0/hTH and pcDNA3.1/hGDNF in SH-SY5Y cells and study their effects on the gene therapy of Parkinson′s disease (PD). Methods pcDNA3.0/hTH and pcDNA3.1/hGDNF were constructed by inserting human TH and GDNF cDNA into pcDNA3.0 and pcDNA3.1, respectively. SH-SY5Y cells were transfected with pcDNA3.0/hTH and pcDNA3.1/hGDNF and the positive cell clones of human TH and GDNF cDNA engineered cells could be identified by RT-PCR. The engineered cells were co-cultured with primary dopaminergic neurons in mouse. The number and growth condition of primary dopaminergic neurons were examined by Immunocytochemistry. Results The number of primary dopaminergic neurons protected by double-gene engineered cells increased at least by 2.8 times in comparison with the control cells(P

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