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1.
Acta Pharmaceutica Sinica ; (12): 2580-2589, 2022.
Article in Chinese | WPRIM | ID: wpr-941522

ABSTRACT

As an essential amino acid, tryptophan (Trp) has various physiological functions and is of great significance in the metabolic process of tumors. In the human body, tryptophan is mainly transformed through kynurenine metabolic pathway, which not only promotes the inherent malignant properties of tumor cells, but also leads to immune-suppressive tumor microenvironment. Changes in tryptophan metabolism often occur in tumors, accompanied by abnormal gene expression of tryptophan-related enzymes, among which indoleamine 2,3-bioxygenase (IDO)-related gene expression and tryptophan 2,3-dioxygenase (TDO)-related gene changes are the most significant. A large number of clinical trials on IDO inhibitors, TDO inhibitors and combination therapy have been carried out. This paper reviewed the tryptophan metabolic pathway, regulation of IDO (TDO), kynurenine (KYN) and other related genes in tumor cells, and outlined the development of therapeutic schedule targeting tryptophan-related genes. The new progress provides new ideas for the further exploration of tumor treatment options.

2.
Chinese Journal of Surgery ; (12): 159-163, 2022.
Article in Chinese | WPRIM | ID: wpr-935595

ABSTRACT

Objective: To examine the modalities of treatment and clinical outcomes of emphysematous pyelonephritis (EPN), in order to improve the survival rate of EPN patients. Methods: Totally 14 patients diagnosed as EPN between October 2011 and November 2020 at Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine were included in this article. Data collection including patient demographics, clinical manifestations, management and clinical outcomes were conducted by retrospective charts review, after receiving the institutional review board's approval. There were 11 females and 3 males with a median age of 59 years (range: 52 to 73 years). The lesions were located on the left side in 10 patients and right side in 4 patients. All the 14 patients suffered from fever, and present with severe sepsis or septic shock. The median time from symptom onset to admission to hospital was 3 days(range: 2 to 5 days). All cases had diabetes mellitus. Escherichia coli was the most common organism been cultured (11 cases), while Klebsiella pneumonia was the second (3 cases). CT scan showed bubbly or located gas in the renal parenchyma in 5 cases and presence of steaky or mottled gas in the renal parenchyma in 9 cases. All patients had been admitted to ICU for anti-septic shock therapy. Three patients had undergone percutaneous catheter drainage along with broad-spectrum antibiotics therapy while 3 patients had immediate nephrectomy, the other 8 cases had a combination of an initial percutaneous catheter drainage and second stage nephrectomy. Results: In this case series, 3 patients were died from EPN while the other 11 were survived. The median ICU stay time was 6 days (range: 3 to 11 days). Of the 3 patients died from EPN, 2 had undergone percutaneous catheter drainage along and 1 had received immediate nephrectomy. Among the 11 patients who were survived, only 1 had received percutaneous catheter drainage while the other 10 received nephrectomy (8 patients had staged nephrectomy). Follow-up was performed 6 months after discharge. Of the 11 surviving patients, 2 were lost to follow-up, and the remaining 9 patients had an creatine level of (118.4±29.4) μmol/L (range: 89 to 176 μmol/L). Conclusions: For patients coupled with diabetes who were initially diagnosed as acute pyelonephritis, the possibility of EPN should be considered when the disease progressed rapidly especially septic shock occurred. On the basis of empirical broad-spectrum antibiotics therapy and standardized anti-septic shock treatment, a combination of an initial percutaneous catheter drainage and second stage nephrectomy could be efficacious.


Subject(s)
Aged , Emphysema/therapy , Escherichia coli Infections , Female , Humans , Male , Middle Aged , Pyelonephritis/therapy , Retrospective Studies , Treatment Outcome
3.
Chinese Journal of School Health ; (12): 776-779, 2022.
Article in Chinese | WPRIM | ID: wpr-934753

ABSTRACT

Objective@#To investigate the prevalence and influencing factors of suicidal ideation and the effects of daily stress and positive mental health on suicidal ideation among college freshmen in Wuhan. This study aims to provide a scientific basis for mental health improvement and suicide prevention among college students from the perspective of positive psychology.@*Methods@#In December 2020, a convenient sampling method was used to select 2 592 freshmen from three universities in Wuhan. Demographic information, suicidal ideation, positive mental health, and daily stress were assessed. T test and Logistic regression analysis were used to analyze the status and influencing factors of suicidal ideation among college freshmen.@*Results@#The one year prevalence of suicidal ideation was 27.5%. Freshmen with suicidal ideation had higher daily stress scores and daily stress scores in different domains than freshmen without suicidal ideation( t =-13.00--4.68), the positive mental health scores of freshmen with suicidal ideation were lower than freshmen without suicidal ideation ( t =17.14, P <0.01). Female ( OR=1.72, 95%CI =1.44-2.05), the higher education level of the mother (OR=1.27, 95%CI=1.05-1.53) and total experience of daily stress ( OR=1.11, 95%CI =1.09- 1.13 ) were associated with risk of oneyear suicidal ideation. Positive mental health was negatively associated with suicidal ideation ( OR=0.88, 95%CI=0.87-0.90, P <0.01) and had a moderating effect on the association between daily stress and suicidal ideation.@*Conclusion@#Suicidal ideation among college freshmen is closely related to daily stress and positive mental health. It is necessary to pay close attention to daily stress of freshmen and reduce suicidal ideation by improving positive mental health.

4.
Article in Chinese | WPRIM | ID: wpr-931348

ABSTRACT

Objective:To assess the reliability, constructive validity and item characteristics of the general self-efficacy scale (GSES) among Chinese medical postgraduates using classical test theory and item response theory.Methods:A questionnaire survey was conducted among 1 021 medical postgraduates from 6 comprehensive level Ⅲ A hospitals in Beijing. Demographic characteristics and general self-efficacy were investigated. SPSS 24.0 software was used for statistical analysis. Internal consistency of the GSES was evaluated using Cronbach’s α coefficient and intraclass correlation coefficient. Factor analyses were implemented to test the structure of the scale. An item response theory (IRT) framework with a graded response model was performed to estimate the parameters of each item. Results:The overall Cronbach’s α coefficient of the GSES was 0.92. Parallel analysis and confirmatory factor analysis supported the unidimensional structure of the GSES. The discrimination parameter of the 10 items ranged from 1.82 to 3.65, and the difficulty parameter ranged from -3.51 to 1.66, with a monotonically increasing trend. The maximum test information of the scale among medical postgraduates was 19.16. Conclusion:The GSES has good reliability, validity and measurement precision, showing a good applicability in the survey of medical postgraduates. However, the difficulty of specific items is relatively low, and the scale may be further optimized and improved.

5.
Article in Chinese | WPRIM | ID: wpr-931096

ABSTRACT

As the number of cataract patients with high myopia increases, and the cataract surgery shifts from a rehabilitation procedure to a refractive procedure, achieving a good postoperative visual acuity has been the target of cataract patients with high myopia.Because of inaccurate axial length measurement, unpredictable effective lens position and improper selection of intraocular lens (IOL) calculation formulas, the precision of refractive prediction in cataract patients with high myopia remains low, which affects the visual acuity and satisfaction of patients.With the development of IOL calculation formulas, SRK/T, Holladay1 and other thin-lens vergence formulas have been modified in axial length, corneal curvature and other parameters, and the thick-lens vergence formulas such as Barrett Universal Ⅱ have been widely put into use.Meanwhile, new formulas including artificial intelligence-based formulas such as Hill-RBF, ray tracing formulas such as Olsen and OKULIX, and theoretical formulas such as Kane and EVO formulas have been developed.More choices and guarantees are available for refractive prediction after cataract surgery.This paper summarized the optimization and advance of different types of IOL calculation formulas so as to provide different available choices to improve the accuracy of IOL power calculation in cataract patients with high myopia.

6.
Article in English | WPRIM | ID: wpr-927677

ABSTRACT

Infectious diseases are an enormous public health burden and a growing threat to human health worldwide. Emerging or classic recurrent pathogens, or pathogens with resistant traits, challenge our ability to diagnose and control infectious diseases. Nanopore sequencing technology has the potential to enhance our ability to diagnose, interrogate, and track infectious diseases due to the unrestricted read length and system portability. This review focuses on the application of nanopore sequencing technology in the clinical diagnosis of infectious diseases and includes the following: (i) a brief introduction to nanopore sequencing technology and Oxford Nanopore Technologies (ONT) sequencing platforms; (ii) strategies for nanopore-based sequencing technologies; and (iii) applications of nanopore sequencing technology in monitoring emerging pathogenic microorganisms, molecular detection of clinically relevant drug-resistance genes, and characterization of disease-related microbial communities. Finally, we discuss the current challenges, potential opportunities, and future outlook for applying nanopore sequencing technology in the diagnosis of infectious diseases.


Subject(s)
Communicable Diseases/diagnosis , High-Throughput Nucleotide Sequencing , Humans , Microbiota/genetics , Nanopore Sequencing , Sequence Analysis, DNA , Technology
7.
Journal of Clinical Hepatology ; (12): 908-912, 2022.
Article in Chinese | WPRIM | ID: wpr-923307

ABSTRACT

Autoimmune hepatitis (AIH) is an inflammatory disease of the liver mediated by autoimmune response, and in the diagnosis and treatment of AIH, it is of great importance to accurately assess the progression of liver inflammation, screen out the patients requiring corticosteroid therapy, and evaluate the therapeutic outcome. This article introduces a variety of new noninvasive techniques which have been discovered by clinical and experimental studies in recent years and have the potential to evaluate the progression of AIH, as well as the advantages and disadvantages of each technique. It is concluded that the new noninvasive techniques have more advantages in guiding the corticosteroid therapy for AIH, but further clinical studies are still needed for verification.

8.
Acta Pharmaceutica Sinica ; (12): 757-765, 2022.
Article in Chinese | WPRIM | ID: wpr-922876

ABSTRACT

UHPLC-Q-TOF/MS metabonomics technology was used to clarify the metabolic regulation pathways by which Platycodon total saponins (PTS) exert antitussive and expectorant effects in a mouse cough model, in which coughing is induced by concentrated ammonia, and in a phenol red excretion model. After approval by the Experimental Animal Ethics Committee of Jiangxi University of Chinese Medicine (Approval No. JZLLSC-20190235), the mice were randomly divided into a normal group, a model group, a positive drug group and a PTS group. Endogenous metabolites in mouse serum were identified by UHPLC-Q-TOF/MS. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used for multivariate analysis. Metabolic pathways were analyzed by the Metaboanalyst platform. The results show that PTS can significantly prolong the cough latent period and cough frequency of mice, and significantly increase phenol red excretion. UHPLC-Q-TOF/MS identified 19 metabolites related to cough, and PTS significantly decreased 16 of them; 17 metabolites related to expectoration were identified, and PTS decreased the levels of all. Metabolic pathway analysis showed that linoleic acid metabolism, arachidonic acid metabolism and glycerophospholipid metabolism were the main pathways involved in serum metabolite changes in this mouse cough model. Linoleic acid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, arachidonic acid metabolism, phenylalanine metabolism and α-linolenic acid metabolism were the main pathways involved in serum metabolite changes in the phenol red excretion model. This study is the first to elucidate the regulation of antitussive and expectorant metabolic pathways and the effect of PTS on these pathways.

9.
China Pharmacy ; (12): 1825-1832, 2022.
Article in Chinese | WPRIM | ID: wpr-936486

ABSTRACT

OBJECTIVE To explore the the reg ulation of intestinal flora and effects of Qingjie huagong decoction on intestinal mucosal barrier in severe acute pancreatitis (SAP)mode rats . METHODS SAP rat model was induced by intraperitoneal injection of caerulein and lipopolysaccharide.The survival state of rats in each group were observed.The levels of serum amylase ,interleukin 10(IL-10),IL-18 and IL- 1β in serum were all detected. The pathological changes of pancreatic and small intestinal tissue were observed. The expressions of Occludin,ZO-1 and HMGB1 were detected in small intestinal tissue of rats. The structure and relative abundance of intestinal microflora in rats were detected by 16S rRNA high throughput sequencing. RESULTS After the intervention of Qingjie huagong decoction ,abdominal distension symptoms of SAP model rats were significantly relieved ,and their mental state recovered better ;the levels of serum amylase and IL- 18 in serum were decreased significantly (P<0.05),while the level of IL- 10 was increased significantly (P<0.05). The necrotic area of pancreatic tissue and the infiltration of inflammatory cells were reduced , the degree of intestinal epithelial cell structural disorder was alleviated ,and the shedding of intestinal mucosal epithelium was reduced.The protein expression of HMGB 1 in small intestinal tissue was decreased significantly (P<0.05),and the protein expression of Occludin and ZO- 1 were increased significantly . Results of 16S rRNA high throughput sequencing showed that Qingjie huagong decoction could increased the relative abundance of probiotics such as Bacteroidea and Lactobacillus in rat intestine ,reduced the colonization of harmful bacteria such as Firmicutes. CONCLUSIONS Qingjie huagong decoction can improve the intestinal barrier by up-regulating the expression of Occludin and ZO- 1 in small intestinal tissue and down-regulating the protein expression of HMGB 1. It can also adjust the relative abundances of different flora to protect the intestinal tract.

10.
International Eye Science ; (12): 975-979, 2021.
Article in Chinese | WPRIM | ID: wpr-876738

ABSTRACT

@#AIM:To explore the effects of recombinant human growth hormone(rHGH)on early rehabilitation in rabbit corneal epithelial wound. <p>METHODS:Thirty-two New Zealand rabbits were selected to establish the corneal epithelial defects models. One eye was treated with normal saline(NC group)and the other eye was treated with 20nmol/L rHGH(rHGH group)in a randomized double-blinded way. The corneal healing process was monitored by the corneal fluorescein staining scores at 0h, 24h, 48h, and 72h after the surgery. The central corneal sensitivity was detected by Cochet-Bonnet corneal esthesiometer and the concentrations of inflammatory mediators interlecukin-1α(IL-1α), interlecukin-17(IL-17), interlecukin-21(IL-21), Leptin, matrix metalloproteinase-9(MMP-9)and tumor necrosis factor-α(TNF-α)in collected tears were measured by multiplex antibody microarray. <p>RESULTS: The corneal epithelial healing rates of the NC group and rHGH group were(62.52±6.73)% and(79.62±10.62)%(<i>P</i><0.05),(90.56±9.57)% and(98.43±3.65)%(<i>P</i><0.05)at 48h and 72h postoperatively. The central corneal sensitivity of rHGH group(4.22±0.26)cm was better than that in NC group(3.22±0.42)cm at 48h after surgery(<i>P</i><0.05). The expressions of TNF-α and IL-1α increased in both groups at each time point after operation, and the expressions in NC group were higher than those in the rHGH group. Both groups had higher MMP-9 concentrations in the tear fluid at 24 and 48h postoperation in comparison with the point before the operation. The MMP-9 expression in NC group was higher than that in the rHGH group at 48h postoperatively. The expressions of IL-21 in NC group were higher than those in the rHGH group at 24 and 48h postoperation in comparison with the point before the operation(<i>P</i><0.05). No significant differences in tearIL-17 and Leptin were observed between groups before and after surgery(<i>P</i>>0.05).<p>CONCLUSION: Topical application of rHGH can accelerate the early stage of rabbit corneal epithelial wound healing <i>in vivo</i>.

11.
Article in English | WPRIM | ID: wpr-880491

ABSTRACT

Biological complexity and the need for personalized medicine means that biomarker development has become increasingly challenging. Thus, new paradigms for research need to be created that bring together a different classifier of individuals. One potential solution is collaboration between biomarker development and Chinese medicine pattern classification. In this article, two examples of rheumatoid arthritis are discussed, including a new biomarker candidate casein kinase 2 interacting protein 1 (CKIP-1) and a micro RNA 214. The authors obtained a "snapshot" of pattern classification with disease in biomarker identification. Bioinformatics analyses revealed underlying biological functions of two biomarker candidates, in varying degrees, are correlated with Chinese medicine pattern of rheumatoid arthritis. The authors' initial attempt can provide a new window for studying the win-win potential correlation between the biomarkers and pattern classification in Chinese medicine.

12.
Article in Chinese | WPRIM | ID: wpr-907335

ABSTRACT

Due to the narrow time window of traditional reperfusion therapy and the presence of the risk of reperfusion injury, it is of great significance to study the pathogenesis of ischemic stroke and explore methods to reduce reperfusion injury from the perspective of pathophysiology. This article expounds the functions of von Willebrand factor (vWF) and platelet glycoprotein (GP) Ⅰb and their roles in hemostasis, thrombosis, and inflammation. It is believed that pharmacological blockade of the interaction of vWF-GP Ⅰb may contribute to the treatment of ischemic stroke. In addition, its clinical significance in ischemic stroke and ischemic brain injury was further discussed.

13.
Article in Chinese | WPRIM | ID: wpr-906519

ABSTRACT

Objective:To explore the clinical efficacy of Shugan Lidan decoction(SLD) combined with laparoscopic cholecystectomy on acute calculous cholecystitis(ACC),and explore its mechanism based on network pharmacology. Method:In a retrospective analysis, 121 patients with ACC were divided into laparoscopic cholecystectomy+SLD group (observation group, 68 cases) and laparoscopic cholecystectomy group(control group,53 cases). The postoperative recovery of the patients (time to first exhaust,time to temperature recovery,antibiotic use time and hospitalization time),serum inflammatory factor levels [white blood cell count(WBC),C-reactive protein(CRP),interleukin-6(IL-6)],immune index levels[claster of differentiation(CD)3<sup>+</sup>,CD4<sup>+</sup>/CD8<sup>+</sup>,immunoglobulin M(IgM)] and safety were compared between these two groups. The TCMSP,BATMAN-TCM and TCMIP databases were used to collect all chemical components and targets of SLD. GeneCard and OMIM databases were combined to search ACC-related targets,and then the intersection ones of SLD-related targets and ACC-related targets were extracted to obtain the potential action targets of SLD for treatment of ACC. The STRING database platform was used to establish and analyze the protein-protein interaction (PPI) network,and Bioconductor software package was used for Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment of the targets. Result:The time to first exhaust,antibiotic use time and hospitalization time of observation group were shorter than those of control group,and the differences were statistically significant(<italic>P</italic><0.05). After treatment,the postoperative WBC,CRP,IL-6,CD4<sup>+</sup>/CD8<sup>+</sup> and IgM in the two groups of patients were lower than those before treatment,and the postoperative CD3<sup>+</sup> was higher than that before treatment,with statistically significant differences(<italic>P</italic><0.05). The postoperative WBC,CRP,IL-6,CD4<sup>+</sup>/CD8<sup>+</sup> and IgM in the observation group were lower than those in control group,and the CD3<sup>+</sup> was higher than that of control group,with statistically significant differences(<italic>P</italic><0.05). There was no statistical difference in adverse reactions between the two groups. Totally 159 components in SLD were screened,which acted synergistically on key targets such as IL-6,vascular endothelial growth factor A(VEGFA),insulin(INS),and epidermal growth factor receptor(EGFR),and participated in the regulation of HIF-1 signaling pathway,EGFR tyrosine kinase inhibitor resistance and PI3K-Akt signaling pathway in the treatment of ACC. Conclusion:SLD may regulate HIF-1 pathway and other signaling pathways by acting on IL-6,VEGFA,INS,EGFR and other targets,thereby reducing postoperative inflammatory factors,improving immune function,and promoting postoperative recovery in patients with ACC.

14.
Article in Chinese | WPRIM | ID: wpr-906306

ABSTRACT

Objective:To investigate the relationship between the single nucleotide polymorphism(SNP)of function genes and effective components of <italic>Salvia miltiorrhiza</italic> and the molecular mechanism of specific quality formation of <italic>S. miltiorrhiza</italic>. Method:The fingerprints of components in <italic>S. miltiorrhiza</italic> from eight different habitats and varieties were obtained by high-performance liquid chromatography (HPLC). The full-length cDNA of three functional genes<italic> </italic>acetyl-CoA C-acetyltransferase(<italic>SmAACT</italic>),4-diphosphocytidyl-2-C-methyl-<italic>D</italic>-erythritol kinase(<italic>SmCMK</italic>) and isopentenyl diphosphate isomerase(<italic>SmIPPI</italic>) in tanshinone metabolic pathway were amplified by polymerase chain reaction(PCR),cloned, and sequenced,followed by bioinformatics analysis. Result:The full-length cDNA sequences of three functional genes <italic>SmAACT</italic>,<italic>SmCMK</italic>, and <italic>SmIPPI</italic> in tanshinone metabolic pathway were obtained from 23 strains of <italic>S. miltiorrhiza</italic> from eight different habitats and varieties. As revealed by the analysis of SNP and amino acid polymorphisms of three functional genes,18,16, and 14 SNP sites were found respectively. HPLC results showed the samples from Beijing,Hubei,Shandong (No. SDB),Shanxi,Henan, and Shandong (No. SDZ) were clustered into one branch,and those from Hebei and Inner Mongolia were clustered into another branch, which suggested that the variation trend of <italic>S. miltiorrhiza</italic> components had little correlation with geographical distance,but the variety was a critical factor for the quality. Conclusion:There was an obvious genetic differentiation trend in <italic>S. miltiorrhiza</italic> from different habitats,and different origin-specific genotypes were formed. The molecular mechanism of the formation of the specific quality of <italic>S. miltiorrhiza</italic> from different habitats was discussed,which laid a foundation for the stability and effectiveness of clinical medication,and guided the breeding of excellent varieties of <italic>S. miltiorrhiza</italic>.

15.
Article in Chinese | WPRIM | ID: wpr-906298

ABSTRACT

Objective:To investigate the effects of oxymatrine(OM) on proliferation,migration, and invasion of non-small cell lung cancer(NSCLC) A549 and H1299 cells and to explore the possible mechanism. Method:A549 and H1299 cells were treated by OM of different concentrations(0, 1.0,2.0,4.0,8.0,16.0, 32.0, and 64.0 mmol·L<sup>-1</sup>) and the cell viability was detected by cell counting kit-8 (CCK-8) assay. Transwell invasion and wound healing assays were applied to determine the effect of OM of different concentrations (8.0,16.0, and 32.0 mmol·L<sup>-1</sup>) on the invasion and migration of A549 and H1299 cells. Western blot was adopted to detect the changes in the expression of proteins related to the Notch signaling pathway after the treatment by OM of different concentrations (8.0,16.0, and 32.0 mmol·L<sup>-1</sup>). Result:Compared with the control,OM could inhibit the proliferation (<italic>P</italic><0.05,<italic>P</italic><0.01) and hinder the cell invasion and migration of A549 and H1299 cells (<italic>P</italic><0.01) in a dose-dependent manner. The results of Western blot showed that OM(32.0 mmol·L<sup>-1</sup>) could effectively counteract the expression levels of Notch1 intracellular domain(NICD),transcriptional complex proteins [TNF-alpha converting enzyme(TACE) and recombining binding protein suppressor of hairless(RBPSUH)], and Hes family hairy and enhancer of split 1(Hes1) in A549 and H1299 cells. Conclusion:OM was capable of inhibiting the proliferation,migration, and invasion of A549 and H1299 cells and also hindering the expression of proteins related to Notch signaling pathway.

16.
Article in Chinese | WPRIM | ID: wpr-921825

ABSTRACT

The human spine injury and various lumbar spine diseases caused by vibration have attracted extensive attention at home and abroad. To explore the biomechanical characteristics of different approaches for lumbar interbody fusion surgery combined with an interspinous internal fixator, device for intervertebral assisted motion (DIAM), finite element models of anterior lumbar interbody fusion (ALIF), transforaminal lumbar interbody fusion (TLIF) and lateral lumbar interbody fusion (LLIF) are created by simulating clinical operation based on a three-dimensional finite element model of normal human whole lumbar spine. The fusion level is at L4-L5, and the DIAM is implanted between spinous process of L4 and L5. Transient dynamic analysis is conducted on the ALIF, TLIF and LLIF models, respectively, to compute and compare their stress responses to an axial cyclic load. The results show that compared with those in ALIF and TILF models, contact forces between endplate and cage are higher in LLIF model, where the von-Mises stress in endplate and DIAM is lower. This implies that the LLIF have a better biomechanical performance under vibration. After bony fusion between vertebrae, the endplate and DIAM stresses for all the three surgical models are decreased. It is expected that this study can provide references for selection of surgical approaches in the fusion surgery and vibration protection for the postsurgical lumbar spine.


Subject(s)
Biomechanical Phenomena , Finite Element Analysis , Humans , Lumbar Vertebrae/surgery , Pedicle Screws , Range of Motion, Articular , Spinal Fusion , Vibration
17.
Article in Chinese | WPRIM | ID: wpr-912817

ABSTRACT

Objective:To analyze the filing mechanism for cross provincial immediate reimbursement of medical insurance in China, so as to provide reference for optimizing the filing mechanism and improving the filing accessibility of insured personnel.Methods:Taking the filing policy of cross provincial immediate reimbursement of medical insurance in 2019 as the research object, on the basis of a comprehensive understanding of the national filing policy background, 90 coordinating regions in Zhejiang Province, Hubei Province and Ningxia Hui Autonomous Region were taken as survey samples to evaluate the relevant policies and extract key parameters, including filing identification methods, filing-related settlement benefits and filing ways. The parameters were compared and analyzed by using descriptive statistical methods.Results:The results of the survey showed that in terms of identity recognition methods, the provision of various supporting materials(residence permit, work certificate, etc.)was still the main way to carry out identity recognition for medical insured persons in different places.Filing-related reimbursement benefits were mainly adjusted by limiting the area of medical insurance treatment and adjusting the benefits parameters(reimbursement ratio). In terms of filing channels, 51(56.7%)sample co-ordination areas had realized at least one remote filing mode.Conclusions:The inclusiveness of filing identity verification mechanism for the floating population needs to be further improved, the filing-related treatment policies need to be further improved, and the convenience and standardization of filing channels need to be strengthened.

18.
Article in Chinese | WPRIM | ID: wpr-912501

ABSTRACT

Objective:To explore the age-related changes of Pole2 expression levels in peripheral blood lymphocytes of healthy people, and analyze the differences of Pole2 expression levels in peripheral blood lymphocytes of four common senile disease patients between normal peers.Methods:Healthy people and patients in the physical examination center of Xuanwu Hospital of Capital Medical University from December 2018 to December 2019 were selected as the research objects, the mRNA and protein level of Pole2 in heathy individual as 20-29,30-39,40-49,50-59,60-69,70-79 y were checked by RT-PCR and Western blot,and the age-related curve was drew. The mRNA and protein expression levels of Pole2 were also detected in the peripheral blood lymphocytes of patients of DM,CHD,AD,CVD. And the deviation with healthy people of the same age was analyzed from one way ANOVA and repeated measurement were used to compare the mean of multiple groups.Results:The mRNA and protein levels of Pole2 increased in the lymphocytes of healthy people at 20-29 y and 50-59 y grouy, and decreased after 60-69 y and 70-79 y ( P<0.001). The Pole2 levels of mRNA and protein in the lymphocytes of patients of CHD,AD,DM and CVD cerebral atherosclerosis were significantly higher than that of healthy people of the same age. Moreover, the Pole2 level of lymphocytes of AD patients was significantly higher than that of other patient groups ( P<0.001). Conclusion:The expression level of Pole2 in peripheral blood lymphocytes increases with age before the age of 60 years old and decreases with age after 60 years old ;and the expression in four common senile diseases was higher than that in normal people.

19.
Article in Chinese | WPRIM | ID: wpr-911745

ABSTRACT

In order to provide efficient medical care to atrial fibrillation patients in the community, the Huamu Community Health Service Center in association with its medical consortium, Renji Hospital have developed a novel atrial fibrillation management system. With the collaboration of general practitioners and specialist team from the tertiary hospital, a special clinic for atrial fibrillation has been set up in the community health service center, which is based on the internet technology and the medical consortium platform. This article introduces the development of this novel system and the initial outcome of the measures, to provide a reference for the management of atrial fibrillation patients in the community.

20.
International Eye Science ; (12): 1644-1648, 2021.
Article in Chinese | WPRIM | ID: wpr-886453

ABSTRACT

@#AIM:To build prediction model of dry eye with data mining techniques.<p>METHODS: From March 2020 to January 2021, 218 patients(436 eyes)with dry eye were selected as dry eye group, and 212 patients(424 eyes)without dry eye were selected as control group. Schirmer Ⅰ test(SⅠt), fluorescein staining tear film break-up time(FBUT), non-contact tear film break-up time(NI-BUT), tear meniscus height(TMH), corneal fluorescein staining(FL)and meibomian gland function score(MG-SCORE)were performed in both groups. Totally 200 eyes of 100 samples were randomly selected from the dry eye group and the control group to form a test set of 400 eyes of 200 samples. The remaining 118 samples(236 eyes)in the dry eye group and 112 samples(224 eyes)in the control group were used as the training set. Correlation feature searching(CFS)feature selection algorithm was used to search the factors related to the detection of dry eye. C4.5, Random Forest, Rondom Tree, Naïve Bayes, KNN, SVM, Decision Stump and Bagging methods were used to construct the prediction model, respectively.<p>RESULTS:By using CFS feature selection algorithm, an optimal sub-feature set including SⅠt, NI-BUT, TMH and FL were obtained. Based on the four features, eight machine learning algorithms were employed to build the prediction model, respectively. The results show that the prediction accuracies were all higher than 75%. Among the eight prediction models, the prediction accuracy model by using Random Forest is the highest, which achieved 91.8% and 88.3%, respectively. And the total prediction accuracy reached 90.1%. In addition, through the analysis of single factor modeling, we found that FL and NI-BUT had the highest prediction accuracy, which exceeded 74%.<p>CONCLUSION: Random Forest could be considered as a stable and well generalization algorithm to build prediction model for dry eye with well generalization. NI-BUT and FL have a strong correlation with dry eye, which can be considered as the standard for clinical examination of dry eye.

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