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1.
China Medical Equipment ; (12): 120-122, 2018.
Article in Chinese | WPRIM | ID: wpr-706472

ABSTRACT

Objective:To explore the countermeasure of strengthening the electronic archives construction of digital medicine in medical industry so as to impel the establishment of electronic archives of digital medicine.Methods: The current situation and improvement of electronic files of digital medicine in medical industry are discussed and the countermeasure in the construction of electric archives of digital medicine was strengthened through analyzing the important role of medical electronic files in the development of hospitals and the existed insufficient in medically electronic file management.Results: The existed shortcomings in the management of medically electronic archives mainly included the lack of precise elaboration and accountability in the existed management system of medically electronic archives, the lack of professional electronic archivists and the hardware facilities should be improved in the management of electronic archives. The countermeasures of strengthening the construction of electronic archives of digital medicine in the medical industry mainly included that improving the management of electronic archives, improving the quality of managers, synchronizing the management of hospital electronic documents and paper documents, strengthening the technical maintenance for electronic archives, increasing the capital investment of electronic archives management and improving the storage of electronic files.Conclusion: Strengthening the construction of electronic files of digital medicine in medical industry is imperative. In order to improve the quality of electronic files, the existed problems in management measures should be clarified and corresponding countermeasures that aimed at problems should be formulated to solve the problems.

2.
Chinese Journal of Neuromedicine ; (12): 226-230, 2013.
Article in Chinese | WPRIM | ID: wpr-1033731

ABSTRACT

Objective To investigate the neuroprotective effect of microRNA-124 on dopaminergic neurons in Parkinson's disease models and its inflammation-related regulation mechanism.Methods The inflammation cell models were prepared by microglial BV2 murine cells; real-time quantitative PCR (qRT-PCR) was performed to analyze the expressions of inflammation-related miRNAs,including miR-21,miR-124,miR-155,miR-146a,miR-181c and miR-221-3p.The C57BL/6 mouse models of Parkinson's disease were established by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MTPT)intraperitoneal injection,and then,administration of miR-124 via tail vein injection was performed;immunohistochemistry was performed to observe the apoptosis of dopaminergic neurons (TH-staining)and the activation ofmicroglial cells (Iba1-staining) in the substantia nigra of animal models before and after treatment; additionally,Western blotting and qRT-PCR were performed to analyze the expressions of the apoptosis-related proteins (caspase-3 and caspase-8).Results As compared with other 5 miRNAs,miR-124 showed significantly higher expression in BV2 cells (P<0.05),and presented higher down-regulation after the induction of inflammatory.As compared with those in the Parkinson's disease models,significantly increased TH-positive cells,decreased Iba1-positive cells and down-regulated expressions of caspase-3 and caspase-8 in the substantia nigra of animal models after miR-124 ministration were observed (P<0.05).Conclusion MiR-124 can slow down the apoptosis of dopaminergic neurons though inhibiting the activation of microglial cells,and maybe it is the key molecule ofpathogenesis of Parkinson's disease.

3.
Acta Pharmaceutica Sinica ; (12): 1053-1059, 2008.
Article in Chinese | WPRIM | ID: wpr-232644

ABSTRACT

This paper is aimed to develop rapid, sensitive and convenient HPLC-MS/MS methods for the quantification of levosimendan and its metabolites OR-1855 and OR-1896 in human plasma. According to the different natures of the compounds, two sets of liquid chromatography and ionization modes were used for determination the concentration of levosimendan and its metabolites OR-1855 and OR-1896 in human plasma, separately. Following protein precipitation with methanol, the levosimendan and internal standard (rosuvastatin) were separated on a Capcell MG III C18 column (35 mm x 2.0 mm ID, 3 microm) with the mobile phase consisted of methanol-15 mmol x L(-1) ammonium acetate-formic acid (55: 45: 0.02, v/v/v). A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated in the negative ion mode. Its metabolites OR-1855, OR-1896 and internal standard doxofylline were extracted from plasma by liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Zorbax Extend C18 column (150 mm x 4.6 mm ID, 5 microm) with the mobile phase consisted of methanol-15 mmol x L(-1) ammonium acetate-formic acid (65 :35 :0.1, v/v/v). A tandem mass spectrometer equipped with electrospray ionization source was used as the detector and operated at the positive ion mode. The linear concentration ranges of the calibration curves for levosimendan and OR-1855 and OR-1896 were 0.10-50.0 ng x mL(-1), 0.20-100 ng x mL(-1), 0.20-100 ng x mL(-1), respectively. The lower limits of quantification of levosimendan and OR-1855 and OR-1896 were 0.10 ng x mL(-1), 0.20 ng x mL(-1), 0.20 ng x mL(-1), respectively. The methods proved to be sensitive, simple and rapid, and suitable for the pharmacokinetic study of levosimendan injection.


Subject(s)
Humans , Male , Acetamides , Blood , Cardiotonic Agents , Blood , Metabolism , Chromatography, High Pressure Liquid , Methods , Hydrazones , Blood , Metabolism , Pyridazines , Blood , Metabolism , Spectrometry, Mass, Electrospray Ionization , Methods , Tandem Mass Spectrometry , Methods
4.
Article in Chinese | WPRIM | ID: wpr-248771

ABSTRACT

<p><b>OBJECTIVE</b>To investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).</p><p><b>METHODS</b>Collecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear. In situ RT-PCR and PCR were used to detect and locate HV RNA and Ot DNA in the primary cultured cells.</p><p><b>RESULTS</b>Positive signals of HV RNA and Ot DNA distributed mostly in epithelial cells of digestive system and ovary cells of larva and nymph. The positive rate increased by the generation of passages.</p><p><b>CONCLUSION</b>Coinfection of HV and Ot did exist in wild Leptotrombidium scutellare.</p>


Subject(s)
Animals , Female , Mice , Cells, Cultured , DNA, Bacterial , Orthohantavirus , Mites , Microbiology , Virology , Orientia tsutsugamushi , RNA, Viral , Reverse Transcriptase Polymerase Chain Reaction
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