Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 14 de 14
Filter
Add filters








Year range
1.
Chinese Medical Journal ; (24): 1337-1346, 2020.
Article in English | WPRIM | ID: wpr-827613

ABSTRACT

BACKGROUND@#Helicobacter pylori (HP) has been considered to be one of the primary causes of gastric mucosa-associated lymphoid tissue (MALT) lymphoma since 1993. Low-grade gastric MALT lymphoma with HP is widely treated with HP eradication therapy, according to each specific clinical situation. However, several studies and guidelines indicate that the modified HP eradication therapy is also valid for HP-negative gastric MALT lymphoma. The aim of this study was to perform a meta-analysis of the clinical efficacy of the modified HP eradication therapy for gastric MALT lymphoma without HP.@*METHODS@#We searched studies that reported the response rate of the modified HP eradication therapy regimen for gastric MALT lymphoma without HP by using PubMed, Medline, and Ebsco from January 1971 until February 2019. All statistical analyses were carried out using R 3.5.3 (Mathsoft Company, Cambridge, MA, USA). The pooled response rate was expressed as a decimal. The heterogeneity test was performed using the I-squared (I) statistic.@*RESULTS@#A total of 14 studies were selected with a total of 148 patients with HP-negative gastric MALT lymphoma. The overall pooled response rate was 0.38 (95% confidence interval [CI]: 0.29-0.47). The combined estimate is I = 57% (P < 0.01). The study subjects were categorized by factors (area of patients). The pooled response rate of the sub-groups (Korea, Japan, China, and Western countries) was 0.63 (95% CI: 0.50-0.76), 0.16 (95% CI: 0.05-0.30), 0.38 (95% CI: 0.20-0.55), and 0.57 (95% CI: 0.08-1.00). The response rate showed that the modified HP eradication therapy was effective for patients with HP-negative gastric MALT lymphoma, especially in Korea and Western countries.@*CONCLUSION@#Therefore, the modified HP eradication therapy can be considered an optional therapy for patients with low-grade HP-negative gastric MALT lymphoma. However, several limitations were revealed in the meta-analysis. Further systematic reviews and research are required.

2.
Article in Chinese | WPRIM | ID: wpr-779547

ABSTRACT

Objective To investigate the accessibility of HIV-related public health services among cross-border couples living in Dehong Prefecture and age differences. Methods A cross-sectional survey was conducted among cross-border couples in Dehong Prefecture from January 2017 to July 2019. Results In total, 32 182 participants were included. The proportion of people who had received HIV testing services, HIV-related intervention services in past year, care and help in the past year, and participated in new rural cooperative medical services (NCMS) were 57.8%, 92.7%, 6.5% and 94.5%, respectively; and the latter three services were significantly different across age groups (P<0.001). In multivariable Logistic regression model, variables significantly associated with having ever received HIV testing services older age (51-85 years: OR=0.71, 95% CI: 0.63-0.81), women (OR=1.14, 95% CI:1.03-1.25), county/city (Longchuan: OR=6.30, 95% CI: 5.72-6.93; Lianghe: OR=1.27, 95% CI: 1.11-1.44; Yingjiang: OR=0.88, 95% CI: 0.82-0.94), Dai ethnic minority (OR=1.60, 95% CI: 1.50-1.72), marriage registration (marriage registration for border inhabitants: OR=0.60, 95% CI: 0.56-0.65; non-registration: OR=0.66, 95% CI: 0.62-0.70), years of marriage (4-5 years: OR=1.21, 95% CI: 1.12-1.31; 6-60 years: OR=1.30, 95%CI:1.22-1.39), having not received care and help in the past year (OR=0.64, 95% CI: 0.58-0.71) and having not participated in NCMS (OR=0.58, 95% CI: 0.52-0.65). Conclusions The accessibility to HIV-related public health services are relatively high among cross-border couples in Dehong Prefecture. However, the relatively low proportion of receiving AIDS testing services, particularly among certain groups and counties/cities, need to be strengthened.

3.
Chinese Journal of Epidemiology ; (12): 220-225, 2012.
Article in Chinese | WPRIM | ID: wpr-269184

ABSTRACT

Objective To describe the genetic characterization of complete genome from a human coxsackievirus A16 (CA16) strain KMM08,isolated in Yunnan,China,in 2008.Methods By using RT-PCR,the seven fragments contained about 1000 nucleotides in the complete genome were sequenced.The sequences were aligned with other enterovirus sequences downloaded from GenBank using Mega 4.1,RDP3 and SimPlot 3.5.1 software.Results As in other human enterovirus,its genome was 7409 nucleotides in length,encoding for 2193 amino acids.KMM08 strain was closely related to other reference strains of B genotype.In the complete genome,the homology of nucleotide and amino acid among the eleven CA16 isolated strains were 79.0%-98.2% and 94.5%-99.3%,respectively.The rates of homology were 79.1% and 94.8% when comparing with that of G10 strains and 78.7% and 89.0% comparing with that of BrCr strains,respectively.SZ-HK08-3 strain had high homology when compared to other strains.In different segment of genome,the rates of homology were 97.0%-99.0% and 98.0%-100.0% when compared with that of SZ-HK08-3 strains,respectively.The rates of homology were 74.2%-86.9% and 90.9%-97.0% when compared with that of G10 strains,respectively and were 65.0%-84.9% and 71.0%-95.2% when compared with that of BrCr strains.Data from Phylogenetic analysis showed that KMM08 belong to genotype B.The putative recombinant Tainan-5079-98 was detected positive with RDP3 and SimPlot 3.5.1.Conclusion KMM08 strains isolated in Yunnan in 2008 belonged to B genotype of coxsackivirus A16.The possible occurrence of inter-typic recombination would involve EV71 and CA16.

4.
Chinese Journal of Pathology ; (12): 662-666, 2012.
Article in Chinese | WPRIM | ID: wpr-303495

ABSTRACT

<p><b>OBJECTIVE</b>To compare the differences in clinicopathologic features of invasive fungal rhinosinusitis caused by Aspergillus and Mucorales, and to discuss the pathogenesis of tissue injury induced by these two kinds of fungi.</p><p><b>METHODS</b>The clinical and pathologic features of 19 patients with invasive fungal rhinosinusitis due to Aspergillus (group A) and 16 patients with invasive fungal rhinosinusitis due to Mucorales (group M) were retrospectively reviewed. HE, PAS and GMS stains were performed on all the paraffin-embedded tissues. The diagnosis was confirmed by histologic examination and microbiological culture results.</p><p><b>RESULTS</b>Amongst the group A patients, the clinical course was acute in 4 cases and chronic in 15 cases. Thirteen cases had underlying predisposing conditions, including diabetes (number = 4), malignant tumor (number = 5), history of trauma (number = 1) and radical maxillary sinus surgery (number = 3). Follow-up information was available in 13 patients. Seven of them died, 4 due to fungal encephalopathy and 3 due to underlying diseases. Amongst the group M patients, the clinical course was acute in 14 cases and chronic in 2 cases. Fourteen cases had underlying predisposing conditions, including diabetes (number = 8), malignant tumor (number = 5) and history of wisdom tooth extraction (number = 1). Follow-up information was available in 14 patients. Four of them died of fungal encephalopathy. There was significant difference in clinical onset between the two groups (P = 0.01). There was however no difference in terms of underlying predisposing conditions and disease mortality. Histologically, the microorganisms in group A patients formed fungal masses and attached to the mucosal surface, resulting in necrotic bands (11/19). Epithelioid granulomas were conspicuous but multinucleated giant cells were relatively rare. Deep-seated necrosis, granulomatous inflammation against fungal organisms (3/19) and vasculitis with thrombosis (4/19) were not common. On the other hand, large areas of geographic necrosis involving deep-seated tissue could be seen in group M patients (13/16). Isolated multinucleated giant cells were commonly seen. Granulomatous inflammation against fungal organisms were identified (16/16). Vasculitis and thrombosis were also observed (10/16).</p><p><b>CONCLUSIONS</b>The invasiveness of Mucorales is remarkable; and when it causes invasive fungal rhinosinusitis, the clinical course is often acute and large areas of tissue necrosis can be seen. The invasiveness of Aspergillus in tissue is relatively mild. Granulomas are more common and the disease often runs a chronic clinical course. There is however no significant difference in long-term mortality. The pathogenesis may be related to the different components of the fungi.</p>


Subject(s)
Adolescent , Adult , Aged , Aspergillosis , Diagnosis , Microbiology , Pathology , Aspergillus , Virulence , Child , Female , Follow-Up Studies , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Mucorales , Virulence , Mucormycosis , Diagnosis , Microbiology , Pathology , Retrospective Studies , Rhinitis , Diagnosis , Microbiology , Pathology , Sinusitis , Diagnosis , Microbiology , Pathology , Young Adult
5.
Chinese Journal of Epidemiology ; (12): 370-375, 2011.
Article in Chinese | WPRIM | ID: wpr-273183

ABSTRACT

Objective To analyze genetic characterization of the small hydrophobic and hemagglutinin-neuraminidase genes of mumps virus(MuV)isolated in Yunnan province,China from 2007 to 2009.Methods Fourteen MuV strains were isolated in Yunnan,China from 2007 to 2009.Using RT-PCR,the SH gene fragments contained 316 nucleotides in all strains and HN gene of six strains were sequenced.The sequences were aligned with other mumps virus sequences downloaded from GenBank using Mega 4.1 software.Results Fourteen isolated strains were closely related to other reference strains of F genotypes.In SH gene,the homology of nucleotide and amino acid among the fourteen isolated strains were 98.3%-100.0%and 96.5%-100.0%,respectively,and 92.6%%-99.4%and 87.7%-100.0% of homology when compared with that of strains isolated from other provinces in China,respectively.Wsh1 and Wsh2 strains had less homology when compared to other strains of F genotypes.The fourteen strains had homology of 84.5%-85.1%and 77.2%compared to vaccine strains on nucleotide and amino acid,respectively,and had homology of 83.4%-90.9% and 70.1%-86.0% compared to that of other genotypes.In HN gene,the homology of nucleotide and amino acid among the six isolated strains were 99.3%-99.5% and 99.1%-99.7%,respectively,and also 99.8% and 99.8% of homology respectively when compared to the SP strain in China.All the six strains had homology of 92.4%-93.2% and 95.5%-96.4% when compared to the vaecine strains on nucleotide and amino acid,respectively,and had homology of 94.7%-96.8% and 95.5%-99.1%compared to other genotypes.Conclusion Fourteen strains isolated in Yunnan from 2007 to 2009belonged to F genotype of MuV while the HN gene seemed more conservative than SH gene.

6.
Chinese Journal of Pathology ; (12): 166-171, 2010.
Article in Chinese | WPRIM | ID: wpr-273410

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features, immunophenotype and ultrastructural features of sinonasal inflammatory myofibroblastic tumors (IMT).</p><p><b>METHODS</b>The clinical and histologic features of 5 cases of sinonasal IMT were reviewed. Immunohistochemical study for vimentin, MSA, SMA, calponin, h-caldesmon, desmin, ALK, fibronectin, CK, S-100 and Ki-67 was carried out. Ultrastructural examination was also performed in two of the cases.</p><p><b>RESULTS</b>The patients age ranged from 28 to 62 years (mean = 43 years). The male-to-female ratio was 2:3. The clinical presentation included nasal obstruction, nasal discharge, nasal bleeding, facial pain, facial swelling, toothache and tear overflow. All of the 5 patients suffered from disease relapses; and 4 of them had recurrences for more than 5 times. One patient had lymph node metastasis and 3 patients died of the disease. Histologically, the tumor cells were arranged in interlacing fascicles and sometimes haphazard in fashion. They were spindly in shape, cytoplasm eosinophilic with mild nuclear atypia and a low mitotic activity. The intervening stroma was myxoid in appearance accompanied by lymphocyte and plasma cell infiltration, abundant blood vessels and focal collagenized areas. In 3 of the recurrent cases, the tumor cells displayed increased nuclear atypia and mitotic activity (average about 5 to 6 per 10 high-power fields), accompanied by patchy necrosis, less inflammatory cell infiltration and focal sarcomatous changes. Immunohistochemical study showed that the tumor cells were diffusely positive for vimentin. SMA, MSA, calponin and fibronectin were variably expressed. Desmin was weakly positive in 1 case. The staining for h-caldesmon, ALK, S-100 and CK was negative. The Ki-67 proliferation index increased with tumor recurrences. Electron microscopy revealed abundant rough endoplasmic reticulum and dense body formation in the cytoplasm. There were an increased amount of collagen fibers in the stroma.</p><p><b>CONCLUSIONS</b>IMT rarely occurs in nasal cavity and paranasal sinuses. The tumor is prone to local invasion and recurrences, with subsequent progression to frank malignancy and distant metastasis, resulting in high mortality and poor prognosis. Complete surgical resection remains the main modality of treatment.</p>


Subject(s)
Actins , Metabolism , Adult , Calcium-Binding Proteins , Metabolism , Diagnosis, Differential , Female , Fibrosarcoma , Pathology , Humans , Ki-67 Antigen , Metabolism , Lymphatic Metastasis , Male , Microfilament Proteins , Metabolism , Middle Aged , Neoplasm Recurrence, Local , Neoplasms, Muscle Tissue , Metabolism , Pathology , General Surgery , Neurofibromatoses , Pathology , Paranasal Sinus Neoplasms , Metabolism , Pathology , General Surgery , Vimentin , Metabolism
7.
Chinese Journal of Pathology ; (12): 508-512, 2010.
Article in Chinese | WPRIM | ID: wpr-333263

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features of malignant tumors in head and neck region complicated by fungal infection.</p><p><b>METHODS</b>Twenty-one cases of malignant tumors occurring in head and neck region complicated by fungal infection were retrieved from the archival file. The light microscopic findings were reviewed. Histochemical (for PAS and GMS) and immunohistochemical (for MUC5B) studies were carried out. Fungal culture results were available in 13 of the 21 cases.</p><p><b>RESULTS</b>The age of the patients ranged from 12 to 72 years (median = 48 years). The male-to-female ratio was 17:4. Eight cases (38.1%) were complicated by invasive fungal sinusitis, with orbital involvement in 6 cases and brain involvement in 1 case. The primary tumors in such cases included leukemia (n = 7) and nasopharyngeal carcinoma (n = 1). The fungi belonged to Zygomycete in 5 cases and Aspergillus in 3 cases. These patients had history of chemotherapy/radiotherapy or antibiotics usage. The remaining 13 cases of fungal infection often affected necrotic tumor tissue in nasal cavity, paranasal sinuses, pharynx, larynx and palate. The fungi involved were Aspergillus (n = 6) and Candida (n = 4). Seven of such patients had received radiotherapy. Fungal culture was positive in 9 cases. Fourteen patients had follow-up information available and six of them died of the disease.</p><p><b>CONCLUSIONS</b>Malignant tumors occurring in head and neck region can be complicated by fungal infection. Invasive fungal sinusitis (due to Zygomycetes and Aspergillus) often occurs in patients with leukemia, tends to involve orbit and is associated with poor prognosis. On the other hand, Aspergillus and Candida are the commonest fungi found in the necrotic tumor tissue. Pathologic examination remains the hallmark in confirming the diagnosis and fungal typing.</p>


Subject(s)
Adolescent , Adult , Aged , Antifungal Agents , Therapeutic Uses , Aspergillosis , Drug Therapy , Microbiology , Pathology , Aspergillus , Candida , Candidiasis , Drug Therapy , Microbiology , Pathology , Carcinoma, Squamous Cell , Drug Therapy , Microbiology , Pathology , Child , Female , Follow-Up Studies , Head and Neck Neoplasms , Drug Therapy , Microbiology , Pathology , Humans , Leukemia , Drug Therapy , Microbiology , Pathology , Lymphoma, Extranodal NK-T-Cell , Drug Therapy , Microbiology , Pathology , Male , Middle Aged , Mycoses , Drug Therapy , Microbiology , Pathology , Retrospective Studies , Sinusitis , Drug Therapy , Microbiology , Pathology , Young Adult , Zygomycosis , Drug Therapy , Microbiology , Pathology
8.
Chinese Journal of Epidemiology ; (12): 460-463, 2007.
Article in Chinese | WPRIM | ID: wpr-294314

ABSTRACT

<p><b>OBJECTIVE</b>To examine the relationships between overweight, obesity and indices as satisfaction of life, trait anxiety, depression, self concept etc. in male and female junior middle school students.</p><p><b>METHODS</b>A questionnaire survey was administered to 1818 participants in a middle school in Hefei city, Anhui province. This contents of survey would include Multidimensional Students' Life Satisfaction Scale (MSLSS), Center for Epidemiologic Studies Depression Scale (CES-D), Trait Anxiety Inventory (TAI), and Piers-Harris Children's Self Concept Scale (PHSCS). Anthropometric measures were taken together with the calculation of body mass index (BMI). The students were divided into normal-weight, overweight and obesity groups by BMI separate criteria. Gender differences of mental health status and group differences of mental health among normal-weight, overweight obesity students were analyzed. Relationships between BMI and the scores of MSLSS, CES-D, TAI, PHSCS were estimated among boys and girls respectively.</p><p><b>RESULTS</b>The prevalence rates of overweight and obesity of boys (21.5%, 7.7%) were significantly higher than in girls (8.0%, 2.8%). Difference of the MSLSS scores in gender was significant among normal-weight group (P = 0.002). The difference of the CES-D scores in gender was significant among overweight group (P = 0.046). There were significant group differences of the CES-D scores, TAI scores, and PHSCS scores among normal-weight, overweight and obese girls (P < 0.05). Both the CES-D score and TAI score of obese girls were higher than normal-weight girls (P = 0.012 and P = 0.035). The PHSCS score of obese girls was lower than normal-weight girls (P = 0.014) and the group difference of the CES-D score was also significant between overweight and normal-weight girls (P = 0.025).</p><p><b>CONCLUSION</b>Both prevalence rates of overweight and obesity of boys were higher than that in girls. Nevertheless, level of mental health level among girls with overweight and obesity were lower than that in boys.</p>


Subject(s)
Adolescent , Body Mass Index , Child , China , Epidemiology , Depression , Epidemiology , Psychology , Female , Humans , Male , Mental Health , Obesity , Epidemiology , Psychology , Overweight , Epidemiology , Psychology , Sex Distribution , Students , Psychology , Surveys and Questionnaires
9.
Chinese Journal of Epidemiology ; (12): 1085-1088, 2007.
Article in Chinese | WPRIM | ID: wpr-322887

ABSTRACT

<p><b>OBJECTIVE</b>To describe the different sex, age and grade distribution of coping behavior and its psychological effects on weight, and to analyze the correlation between overweight, obesity and unhealthy psycho-behaviors related to weight control in adolescents.</p><p><b>METHODS</b>A survey was conducted among students of grade 1 and grade 2 at a junior middle school in Hefei. Students completed the anonymous. questionnaire including the demographic characteristics of adolescents and parents, self-assessed weight status and adolescents' psycho-behaviors related to weight control. Anthropometric measures were taken together with body mass index (BMI) calculated. Students were divided into obesity,overweight and control groups by BMI separate criteria and by self reporting, respectively.</p><p><b>RESULTS</b>Among all the students, 17.4% reported having unhealthy psychological feelings related to weight control including 3.8% reported having unhealthy behaviors related to weight control and 19.1% of them reported having unhealthy psycho-behaviors related to weight control. The rates of unhealthy psychological feelings, unhealthy behaviors and total unhealthy psycho-behaviors related to weight control among girls (20.6%, 4.3%, 22.6%) were all higher than those among boys (14.5%, 3.3%, 15.8%) (P < 0.05). The rates of unhealthy psychological feelings, unhealthy behaviors and total unhealthy psycho-behaviors related to weight control in obese individuals both assessed by BMI and assessed by self reporting appeared to be the highest (P < 0.05). In addition,data showed that obesity and overweight assessed both by BMI separate criteria and by self-reporting appeared positive correlations with the rates of unhealthy psycho-behaviors related to weight control. Moreover, the relationship between obesity or overweight assessed by the two methods and unhealthy psycho-behaviors related to weight control did not alter by other factors.</p><p><b>CONCLUSION</b>Unhealthy psycho-behaviors related to weight control of girls are more severe than boys. The relationship between obesity and unhealthy psychological feelings and behaviors related to weight control does not alter by other factors such as social factors.</p>


Subject(s)
Adolescent , Adolescent Behavior , Psychology , Body Mass Index , Female , Health Behavior , Humans , Male , Obesity , Psychology , Overweight , Psychology , Students , Psychology , Surveys and Questionnaires
10.
Article in Chinese | WPRIM | ID: wpr-686096

ABSTRACT

Objective:Establish mass-scale purification technology of cell-derived influenza virus. Methods: A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results: The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion: Providing a high effective purification technology for cell-derived influenza virus.

11.
Article in Chinese | WPRIM | ID: wpr-239073

ABSTRACT

<p><b>OBJECTIVE</b>To observe the thick basement membrane-like layer under the damaged epithelium in patients with chronic rhinosinusitis (CRS) and to study its principal components.</p><p><b>METHODS</b>Patients (n = 40) with CRS undergoing functional endoscopic sinus surgery were recruited for the study. Patients (n = 10) with no evidence of sinus disease were used as control subjects. Ethmoidal sinus mucosa specimen were stained by PAS staining to measure the thickness of basement membrane-like layer. Immunohistochemical staining, picrosirius-polarization method and transmission electron microscopy (TEM) were used to analyse the nature of the layer.</p><p><b>RESULTS</b>The thickness of basement membrane-like layer in CRS type I and CRS type II were significantly increased compared with normal control subjects (t = 5. 426, P < 0. 01; t = 8. 375, P < 0.01). The thickness of CRS type II was significantly greater compared with CRS type I (t = 3. 908, P < 0.01). There was no difference between atopic group and inotropic group (t = 0.803, P> 0.05). There was no expression of fibronectin, laminin, IgA, IgG and IgM in the thick basement membrane-like layer. A thick reticular structure containing collagen fibrils and devoid of cellular elements was observed by TEM.</p><p><b>CONCLUSIONS</b>This study demonstrates that a long-standing chronic inflammation leads to deposition of collagens type I , III and IV under the damaged epithelium, resulting in an increasingly thick sub-basement membrane layer.</p>


Subject(s)
Adolescent , Adult , Aged , Basement Membrane , Metabolism , Pathology , Case-Control Studies , Chronic Disease , Collagen , Metabolism , Female , Humans , Male , Middle Aged , Nasal Mucosa , Metabolism , Pathology , Sinusitis , Metabolism , Pathology , Young Adult
12.
Article in Chinese | WPRIM | ID: wpr-258748

ABSTRACT

<p><b>OBJECTIVE</b>To observe the relationship between histomorphological changes in mucosa of chronic sinusitis (CS) and aerosol particles.</p><p><b>METHODS</b>The ethmoidal sinus mucus specimens of 60 patients with CS type I, II and III, and 16 cases of normal controls were collected. Meanwhile the pulmonary specimens in autopsy of 5 adult and 5 fetus cases were also collected. The specimens were stained by HE staining and Warthin-Starry (W-S) special aerosol particles staining method respectively, the ethmoidal sinus mucosa basement membrane were displayed by Van Gieson's (VG) special staining method, and its thickness was measured.</p><p><b>RESULTS</b>By the W-S staining, some black aerosol particles were found depositing on the epithelial cells layer of damaged mucosa and in the plasm of the epithelial cells. The degree of deposition was gradually increased in type I, II and III of CS. The number of cases of slight, medium and heavy depositing in type I were 11/20, 7/20, 2/20, in type II 5/20, 9/20, 6/20, and in type III 3/20, 6/20, 11/20 respectively; the more particles deposited, the more thickening the basement membrane. The thickness of the basement membrane in type I, II and III of CS were (12.15 +/- 4.73), (16.67 +/- 2.22), (23.75 +/- 3.48) microm respectively. There were significant differences among type I, II and III (P < 0.01). The black particles in the mucosa epithelium tissues of normal control group and non-damaged mucosa epithelium and desquamation area were seldom seen. On the other hand, the aerosol particles were not seen in the pulmonary specimens of the fetal autopsy.</p><p><b>CONCLUSION</b>In damaged mucosa epithelial cells of the CS, there are aerosol particles existing which may relate with the morphological changes of CS.</p>


Subject(s)
Adolescent , Adult , Aged , Air Pollutants , Chronic Disease , Ethmoid Sinus , Pathology , Female , Humans , Male , Middle Aged , Nasal Mucosa , Pathology , Sinusitis , Pathology , Staining and Labeling
13.
Chinese Journal of Pathology ; (12): 432-436, 2003.
Article in Chinese | WPRIM | ID: wpr-242158

ABSTRACT

<p><b>OBJECTIVE</b>To observe the common characteristics of pathological morphology and immunohistochemical staining of olfactory neuroblastoma (ONB), and to raise the diagnostic ability for ONB.</p><p><b>METHODS</b>34 cases of ONB, 11 cases of rhabdomyosarcoma (RMS) and 76 cases of malignant lymphoma (ML) were collected, the clinical information were investigated, and the biopsy samples were stained and observed as follows: (1) Routine HE staining and viewed under the light microscope. (2) Expression of neuron-specific enolase (NSE), chromogranin-A (CgA), leukocyte common antigen (LCA), desmin, and sarcometic actin (S-actin) were determined in both ONB and RMS cases. In addition, S-100 protein, cytokeratin (AE1/AE3) were detected in the ONB and myoglobulin detected in the RMS. ML samples were stained for LCA, CD45RO, CD56, and CD20. The NSE, CgA, desmin and S-actin were stained in 10 cases of NK/T cell ML and 9 cases of B cell ML additionally. (3) 4 cases of ONB, RMS and ML were observed under transmission electron microscope respectively.</p><p><b>RESULTS</b>The ages and clinical manifestations of ONB, RMS and ML were similar. The morphological characteristics of ONB included epithelial nests; net of angioma-like fibrous connective tissues; small round cells and small short spindle cells and nucleus; glandular and squamous liked epithelium; Homer-Wright and Flexner rosette; bunch of neurofibrilla, etc. NSE and CgA were expressed in small cells. S-100 protein was positive in the area of bunch of neurofibrilla. AE1/AE3 was positive in epithelial cells, LCA, while desmin and S-actin were all negative. Ultrastructurelly, there were neurosecretory granules and neurofibrilla in the cytoplasm of a few tumor cells. Although there were some similarities among ONB, RMS and ML under the light microscope, their characteristics of pathologic morphology, immunohistochemical staining and transmission electron microscope were different.</p><p><b>CONCLUSIONS</b>The features of the morphological changes are the most important basis to make diagnosis for ONB. The results of immunohistochemical staining can verify it further and play an important role in its differential diagnosis. Transmission electron microscope is very important but not essential for its diagnosis.</p>


Subject(s)
Adolescent , Adult , Aged , Child , Diagnosis, Differential , Esthesioneuroblastoma, Olfactory , Diagnosis , Pathology , Female , Humans , Immunohistochemistry , Male , Middle Aged , Nasal Cavity , Nose Neoplasms , Diagnosis , Pathology , Rhabdomyosarcoma , Diagnosis , Pathology
14.
Article in Chinese | WPRIM | ID: wpr-676529

ABSTRACT

Objective To observe the effect of berberine on visfatin expression in 3T3-L1 adipocytes,and to explore the mechanism underlying berberine improving insulin resistance.Methods Visfatin mRNA and protein expressions were assayed by RT-PCR and Western blot after incubation of berberine at different concentrations and durations with 3T3-L1 adipocytes.Results In 3T3-L1 adipocytes,visfatin mRNA expression was increased at 0- 10?mol/L berberine with the maximum effect at 10?mol/L,being 4.96 folds of control group.While as the berberine concentrations were further increased above 10?mol/L,the visfatin mRNA level decreased.After incubation with berberine for 3 h,the visfatin mRNA level began to increase,and reached the peak when incubation for 12 h(4.57 fold of basal group).Then the mRNA level decreased,though it was still higher than that in control group after 48h intervention.5,10, 20?moL/L berberine could increase visfatin protein expression by 1.13,2.46,2.34 folds respectively as compared with control group(P

SELECTION OF CITATIONS
SEARCH DETAIL