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1.
Article in Chinese | WPRIM | ID: wpr-940455

ABSTRACT

ObjectiveTo investigate the inhibitory effect of Astragalus polysaccharide (APS) on epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β1 (TGF-β1) in cisplatin (DDP)-resistant lung adenocarcinoma cell line A549/DDP cells transplanted into nude mice and the molecular mechanism in improving DDP resistance. MethodBALB/c nude mice were randomly divided into a blank group, a model group, a DDP group, and a combination group (APS combined with DDP). A549/DDP cells were infected with TGF-β1-overexpressed lentiviral vector and the negative control. The infected cells were inoculated subcutaneously in nude mice. The A549/DDP cells with TGF-β1 gene overexpression were inoculated into all groups except the control group with negative TGF-β1 gene overexpression. The drug intervention was performed eight days after cell inoculation. The mice in the combination group received intragastric administration of APS (0.3 g·kg-1·d-1) and intraperitoneal injection of cisplatin (0.003 5 g·kg-1), and those in the cisplatin group received intraperitoneal injection of cisplatin (0.003 5 g·kg-1). After 32 days of cell inoculation, the nude mice were killed and the tumor tissues and lungs were collected. The tumor weight was recorded and the inhibition rate was calculated. The number of metastatic nodules of the lung tumor on the whole slide was counted under the microscope. Immunohistochemistry, Western blot, and real-time fluorescence-based quantitative polymerase chain reaction (Real-time PCR) were used to detect the protein and gene expression of EMT molecular markers α-catenin and N-cadherin, and tumor drug resistance markers human lung resistance protein (LRP), multidrug resistance-associated protein (MRP), and P-glycoprotein (P-gp) in the transplanted tumor. ResultCompared with the blank group, the model group showed increased tumor weight and metastatic nodules of the lung tumor (P<0.05), decreased protein and mRNA expression of α-catenin (P<0.05), and elevated protein and mRNA expression of N-cadherin, LRP, MRP, and P-gp (P<0.05). Compared with the model group and the cisplatin group, the combination group showed reduced tumor weight and metastatic nodules of the lung tumor (P<0.05), increased protein and mRNA expression of α-catenin (P<0.05), and decreased protein and mRNA expression of N-cadherin, LRP, MRP, and P-gp (P<0.05). ConclusionAPS can inhibit the growth and metastasis of the transplanted tumor of lung adenocarcinoma and improve cisplatin resistance, which may be related to the inhibition of EMT of tumor cells.

2.
Article in Chinese | WPRIM | ID: wpr-905862

ABSTRACT

Objective:To investigate the effect of Astragalus polysaccharide (APS) on transforming growth factor-<italic>β</italic><sub>1</sub> (TGF-<italic>β</italic><sub>1</sub>)-induced epithelial mesenchymal transition (EMT) of A549/DDP lung adenocarcinoma xenograft and its potential molecular mechanism. Method:BALB/c nude mice were randomly divided into the non-loading group (A549/DDP cells not loaded with TGF-<italic>β</italic><sub>1</sub>), model group, cisplatin group, and combined group (A549/DDP cells overexpressing TGF-<italic>β</italic><sub>1</sub>). Mice in the combined group were treated with intragastric administration of APS (0.3 g·kg<sup>-1</sup>·d<sup>-1</sup>) and intraperitoneal injection of cisplatin (0.003 5 g·kg<sup>-1</sup>), while those in the cisplatin group only received intraperitoneal injection of cisplatin (0.003 5 g·kg<sup>-1</sup>). After drug intervention, the nude mice were sacrificed and the xenograft and lung were harvested, followed by the weighing of tumor and the calculation of the inhibition rate. The number of tumors metastasizing to the lung was counted under the microscope. The pathological features of tumors and their metastasis to the lung tumor were observed by hematoxylin-eosin (HE) staining. The protein and mRNA expression levels of EMT molecular markers E-cadherin, Vimentin, <italic>α</italic>-smooth muscle actin (<italic>α</italic>-SMA), and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) in the xenograft were detected by immunohistochemistry, Western blot, and Real-time polymerase chain reaction (Real-time PCR). Result:Compared with the non-loading group, the model group exhibited increased tumor weight and pulmonary metastatic nodules (<italic>P</italic><0.05), sparse tumor cell junctions, long spindle cells, massive metastatic nodules in the lung, down-regulated E-cadherin protein and mRNA expression, and up-regulated Vimentin and <italic>α</italic>-SMA protein and mRNA expression and p-PI3K and p-Akt protein expression (<italic>P</italic><0.05). Compared with the model group and cisplatin group, the combined group displayed decreased tumor weight and pulmonary metastatic nodules (<italic>P<</italic>0.05), tight tumor cell junctions, round or oval cells, no obvious lung metastasis, up-regulated E-cadherin protein and mRNA expression (<italic>P</italic><0.05), and down-regulated Vimentin and <italic>α</italic>-SMA protein and mRNA expression (<italic>P</italic><0.05) and p-PI3K and p-Akt protein expression (<italic>P</italic><0.05). There was no significant difference in PI3K or Akt protein expression among groups. Conclusion:APS has a certain inhibitory effect against EMT in lung adenocarcinoma A549/DDP cells, which may be related to the inhibition of activated PI3K/Akt protein expression.

3.
Article in Chinese | WPRIM | ID: wpr-879867

ABSTRACT

OBJECTIVE@#To study the effect of astragaloside IV (AS-IV) on NOD-like receptor protein 3 (NLRP3) inflammasome in neonatal rats with hypoxic-ischemic brain damage (HIBD).@*METHODS@#A total of 24 Sprague-Dawley rats, aged 7 days, were randomly divided into a sham-operation group, an HIBD group, and an AS-IV treatment group, with 8 rats in each group. After 24 hours of modeling, brain tissue was collected for hematoxylin-eosin staining, yo-PRO-1 staining, and EthD-2 immunofluorescent staining in order to observe the cerebral protection effect of AS-IV in vivo. HT22 cells were used to prepare a model of oxygen-glycogen deprivation (OGD), and a concentration gradient (50-400 μmol/L) was established for AS-IV. CCK-8 assay was used to measure the viability of HT22 cells. RT-PCR and Western blot were used to observe the effect of different concentrations of AS-IV on the mRNA and protein expression of NLRP3, gasdermin D (GSDMD), caspase-1, and interleukin-1β (IL-1β).@*RESULTS@#Yo-Pro-1 and EthD-2 staining showed that compared with the sham-operation group, the HIBD group had an increase in pyroptotic cells with a small number of necrotic cells, and the AS-IV group had reductions in both pyroptotic and necrotic cells. Compared with the sham-operation group, the HIBD group had significantly higher protein expression levels of NLRP3, IL-1β, caspase-1, and GSDMD (@*CONCLUSIONS@#AS-IV may alleviate HIBD in neonatal rats by inhibiting the expression of NLRP3, GSDMD, caspase-1, and IL-1β.


Subject(s)
Animals , Animals, Newborn , Brain , Hypoxia-Ischemia, Brain/drug therapy , Inflammasomes , NLR Proteins , Rats , Rats, Sprague-Dawley , Saponins , Triterpenes
4.
Article in Chinese | WPRIM | ID: wpr-801765

ABSTRACT

Objective: To investigate the effect of Buzhong Yiqi Tang on cisplatin resistance tumor cells A549/DDP in nude mice with lung adenocarcinoma, and determine the optimal concentration. Method: The 36 BALB/C nude mice were randomly divided into no-load group, model group, cisplatin group, low-dose Buzhong Yiqi Tang group (1.46 g · kg-1), medium-dose Buzhong Yiqi Tang group (2.92 g · kg-1) and high-dose Buzhong Yiqi Tang group (5.84 g · kg-1). The no-load group was inoculated with A549/DDP cells transfected by uninfected lentiviral transforming growth factor-β1(TGF-β1), and the other groups were inoculated with TGF-β1 over-expression A549/DDP cells. After the intervention, the nude mice were put to death. The tumors of mice were weighed, and the inhibition rate was calculated, liver, spleen and lung metastases of tumors were counted under microscope, and pathological observation was made for lung histomorphological changes. Immunohistochemistry, Western blot and Real-time polymerase chain reaction(Real-time PCR) were used to detect the expressions of lung resistance-related protein (LRP), multidrug resistance-related protein (MRP), P-glycoprotein (P-gp) in transplantation tumor. Result: Compared with the model group, the weight of tumors and the number of pulmonary metastatic nodules decreased in the Buzhong Yiqi Tang intervention group. With the increase of the dosage of Buzhong Yiqi Tang, the weight of tumors gradually decreased, while the inhibition rate increased. The effect of the high-dose group was the most significant (PPPConclusion: Buzhong Yiqi Tang can inhibit the growth and metastasis of transplanted tumors, alleviate lung injury and improve the drug resistance of A549/DDP to cisplatin, with a certain dose-effect relationship. High-dose concentration (5.84 g ·kg-1) was the best concentration in nude mice.

5.
Chinese Journal of Pathophysiology ; (12): 1124-1128, 2018.
Article in Chinese | WPRIM | ID: wpr-701250

ABSTRACT

AIM:To observe the expression of Akt/GSK-3β/Snail signaling pathway-related molecules in cis-platin-resistant cell line A549/DDP mediated by transforming growth factor-β1 (TGF-β1), and to explore the association of Akt/GSK-3β/Snail signaling pathway with epithelial-mesenchymal transition (EMT). METHODS:The A549/DDP cells were divided into TGF-β1(+) group, TGF-β1(-) group and LY294002 group. The morphological changes of A549/DDP cells treated with TGF-β1 were observed under microscope. The protein expression of E-cadherin and N-cadherin was determined by the methods of immumofluorescence and Western blot. The protein levels of Akt, p-Akt, GSK-3β, p-GSK-3βSer9 and Snail were also detected by Western blot. RESULTS:The A549/DDP cells in TGF-β1(+) group were disper-sive, showed a spindle-like shape and developed pseudopodia. This transformation was conformed to classic EMT markers. Compared with TGF-β1(-) group, the protein expression of E-cadherin in TGF-β1(+) group was significantly decreased (P<0.05), and N-cadherin was significantly increased (P<0.05). The protein levels of p-Akt, p-GSK-3βSer9 and Snail were also significantly increased ( P<0.05). Compared with TGF-β1(+) group, the protein levels of p-Akt, p-GSK-3βSer9 and Snail were significantly decreased in LY294002 group (P<0.05). No difference of Akt and GSK-3β expression between TGF-β1(-) group and TGF-β1(+) group was observed. CONCLUSION:The mechanism of EMT in A549/DDP cells might be related to Akt/GSK-3β/Snail signaling pathway activated by TGF-β1.

6.
Article in Chinese | WPRIM | ID: wpr-699465

ABSTRACT

Objective To observe the expression of p57,CD34 and Ki-67 in complete hydatidiform mole (CHM),partial hydatidiform mole (PHM) and hydropic abortus (HA),so as to investigate the role of the expression in the diagnosis and differential diagnosis of edematous lesions of placental villi.Methods A total of 45 cases of CHM tissue,40 cases of PHM tissue,28 cases of HA tissue and 22 cases of normal pregnancy tissue were collected from January 2003 to December 2013 in Anqiu People's Hospital.The expression of p57,CD34 and Ki-67 in placental villi were detected by immunohistochemistry.Results The positive expression rate of p57 in CHM,PHM,HA and normal pregnancy tissues was 2.22% (3/45),85.00% (34/40),89.29% (25/28) and 95.45 % (21/22),respectively;the positive expression rate of p57 in CHM tissues was significantly lower than that in PHM,HA and normal pregnancy tissues (x2 =59.908,57.055,58.238;P < 0.01);there was no significant difference in the positive expression rate of p57 in PHM,HA and normal pregnancy tissues (x2 =0.022,0.681,0.074;P >0.05).The expression of CD34 in CHM,PHM,HA and normal pregnancy tissues was 10.27 ± 3.00,11.13 ±2.58,35.57 ± 2.36 and 35.55 ± 2.22 respectively;the expression of CD34 in CHM and PHM tissues was significantly lower than that in HA and normal pregnancy tissues (t =37.89,37.86,39.79,37.40;P < 0.01).There was no significant difference in the expression of CD34 between HA and normal pregnancy tissues (t =1.485,P > 0.05),and there was no significant difference in the expression of CD34 between CHM and PHM tissues (t =1.404,P > 0.05).The positive expression rate of Ki-67 in CHM,PHM,HA and normal pregnancy tissues was 64.44% (29/45),55.00% (22/40),14.29% (4/28) and 9.09% (2/22) respectively;the positive expression rate of Ki-67 in CHM and PHM tissues was significantly higher than that in HA and normal pregnancy tissues (x2 =18.21,12.61,17.53,11.56;P < 0.01);there was no significant difference in the positive expression rate of Ki-67 between HA and normal pregnant tissues (x2 =0.015,P > 0.05),and there was no significant difference in the positive expression rate of Ki-67 between CHM and PHM tissues (x2 =0.787,P > 0.05).Conclusion p57 helps to identify CHM and other edematous lesions of placental villi,CD34 and Ki-67 help to identify hydatidiform mole and HA.Combined detection of p57,CD34 and Ki-67 is helpful for differential diagnosis of CHM,PHM and HA.

7.
Article in Chinese | WPRIM | ID: wpr-275471

ABSTRACT

This study was aimed to explore the effects of Buzhong Yiqi decoction on the expression levels of Bad, NF-κB, caspase-9, Survivin, and mTOR in nude mice with A549/DDP transplantation tumors.Sixty BALB/C mice were randomly divided into blank control group, tumor-bearing control group, cisplatin group and Buzhong Yiqi decoction of high, medium and low doses+cisplatin groups (hereinafter referred to as the high,medium and low combined groups). A549/DDP cells (concentration of 5×106 cells/mL)were cultured and inoculated in various groups, then the tumor-forming situations were observed. Corresponding treatment was given in all groups. Fourteen days later, immunohistochemistry and Real-time PCR methods were used to detect the expression levels of Bad, NF-κB, caspase-9, Survivin, mTOR protein and mRNA in tumors.Results showed that Buzhong Yiqi decoction combined with cisplatin could reduce the volume of transplanted tumors, and there was significant difference between medium combined group and high combined group(P<0.05). As compared with the tumor-bearing control group, the expression levels of Bad, NF-κB, Survivin and mTOR were significantly reduced in medium and high combined groups(P<0.05); the protein and mRNA expression levels of caspase-9 were gradually increased in medium combined and high combined groups(P<0.05), with statistical difference with tumor-bearing control group(P<0.05). There were statistical difference in mRNA expression of Bad, NF-κB and caspase-9 between medium combined group, high combined group and cisplatin group, low-combined group, tumor-bearing control group(P<0.05), but there was no statistical difference between cisplatin group, low-combined group, and tumor-bearing control group. In addition, there was no statistical difference between medium combined group and high combined group in protein and mRNA expression levels of various factors. Experimental results showed that Buzhong Yiqi decoction combined with cisplatin can inhibit the growth of A549/DDP transplanted tumors, and the mechanism may be associated with regulating Bad, NF-κB, caspase-9, Survivin, and mTOR levels as well as promoting apoptosis.

8.
Article in Chinese | WPRIM | ID: wpr-850140

ABSTRACT

Objective To investigate the protective effect of prostaglandin E2 (PGE2), cyclooxygenase-2 (COX-2) and platelet activating factor (PAF) receptor antagonist on endotoxin-induced acute gastric mucosal injury in young rats. Methods Eighteen-day old Wistar rats were randomly divided into 4 groups: normal control group, model group (LPS group), PAF antagonist prevention group, and PAF antagonist treatment group. The model of endotoxemia in young rats was reproduced by intraperitoneal injection of endotoxin (5mg/kg of O55:B5 lipopolysaccharide). The rats in PAF prevention and treatment group received PAF antagonist BN52021 (Ginkgolide B, 5mg/kg) 0.5h before or after modeling. The rats in control group were given intraperitoneal injection of same amount of normal saline (1ml/kg). The animals were sacrificed 1.5, 3, 6, 24, 48 and 72h after intraperitoneal injection of endotoxin (8 in each group). The pathologic changes in gastric mucosa were observed after HE staining. The content of PGE2was measured by radioimmunoassay, the expression of COX-2 protein was determined by immunohistochemistry SP method, and the expression of COX-2 mRNA was assessed with RT-PCR method. Results Pathological changes in gastric mucosa were found to be edema of epithelial cells at 1.5h, and hyperemia and edema 3h after intraperitoneal injection of endotoxin in LPS group. The changes were most marked at 6h, including bleeding, karyorrhexis, pyknosis and apoptosis of epithelial cells of gastric mucosa. Exfoliation of the epithelium and neutrophil infiltration were observed at 24h, thinning of mucosa and a decrease in glands were observed at 48h, but no further changes were observed at 72h. However, all the above changes were significantly alleviated in prevention and treatment groups. The PGE2 content of gastric mucosa was lowered at 3h (P<0.05), and it was lowest at 6h (P<0.01) after endotoxin injection in LPS group, and significant difference was found between LPS group and control group. The PGE2 content of gastric mucosa was obviously increased at 3h and 6h in prevention group (P<0.05), and at 6h in treatment group (P<0.05). The differences at 6h were significant (P<0.01) among prevention group, treatment group and LPS group. No expression of COX-2 protein or mRNA was seen in gastric mucosal tissue of control group. In contrast with control group, cytoplasm COX-2 protein of gastric mucosal tissue was seen to express at 6h after endotoxin injection in LPS group, and it was obviously enhanced at 24, 48 and 72h (P<0.01), and the COX-2 mRNA level was also elevated. The expressions of COX-2 protein and mRNA were increased obviously at 6h in PAF antagonist prevention group and treatment group (P<0.01). The expressions of COX-2 protein at 6h and COX-2 mRNA at 24h were obviously elevated in prevention group and treatment group compared with those of LPS group (P<0.01). Conclusion PAF receptor antagonist may up-regulate the expression level of COX-2 protein and mRNA, increase PGE2 content, alleviate acute gastric mucosal injury, and promote the healing of gastric mucosal injury.

9.
Article in Chinese | WPRIM | ID: wpr-327905

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of Buzhong Yiqi decoction on PI3K/AKT signaling pathway in spleen, stomach and lung of nude mice with lung adenocarcinoma transplantation tumor.</p><p><b>METHOD</b>Totally 60 nude mice were randomly divided into the blank control group, the tumor-bearing control group, the cisplatin group, the low-dose Buzhong Yiqi decoction group, the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group. After the corresponding interventions, efforts were made to measure the transplanted tumor volume and calculate the tumor inhibiting rate. The immunohistochemical method and real time PCR were used to detect the expression of PI3K and AKT level in nude mice spleen, stomach and lung.</p><p><b>RESULT</b>Buzhong Yiqi decoction of different concentrations combined with cisplatin could inhibit the growth of the transplanted tumor, with the strongest inhibitory effect in the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group. All of the expressions of PI3K and AKT protein and gene in the spleen, stomach and lung increased, with the most significant increase in the tumor-bearing group. Along with the increase of the concentration of cisplatin and Buzhong Yiqi decoction, the expressions of PI3K and AKT gradually reduced. Compared with the tumor-bearing control group, there were statistical differences in spleen and stomach tissues (P < 0.05). Compared with the cisplatin group, the middle-dose Buzhong Yiqi decoction group and the high-dose Buzhong Yiqi decoction group showed statistical differences (P < 0.05), but without statistical difference compared with the blank control group.</p><p><b>CONCLUSION</b>Among nude mice with lung adenocarcinoma transplantation tumor, the PI3K and AKT protein and gene expressions in spleen, stomach and lung tissues increased, which might indicated the effect of cisplatin and Buzhong Yiqi decoction in reducing PI3K and AKT expressions and the relations between the reduction degree and the concentrations of Buzhong Yiqi decoction. Cisplatin combined with Buzhong Yiqi decoction could decrease the PI3K and AKT protein and gene expression in spleen, stomach and lung, and make the pathway closer to normal, so as to protect the functions of spleen, stomach and lung, there may be target spots of Buzhong Yiqi decoction in PI3K/AKT signal pathway.</p>


Subject(s)
Adenocarcinoma , Drug Therapy , Genetics , Animals , Cell Line, Tumor , Drugs, Chinese Herbal , Female , Humans , Lung , Lung Neoplasms , Drug Therapy , Genetics , Male , Mice , Mice, Inbred BALB C , Mice, Nude , Oncogene Protein v-akt , Genetics , Metabolism , Phosphatidylinositol 3-Kinases , Genetics , Metabolism , Signal Transduction , Spleen
10.
Article in Chinese | WPRIM | ID: wpr-313043

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the reversal effect of Buzhong Yiqi Decoction (BYD) on multidrug resistance of human adenocarcinoma of lung cell line A549/DDP, and to study its effect on the expression of survivin by using serum pharmacological methods in vitro. Methods Totally 24 SD rats were divided into the high, medium and low dose groups, and the blank control group by randomized controlled method. The high dose BYD containing serum (1. 134 g/mL, 2 mL), the middle dose BYD containing serum (0.576 g/mL, 2 mL), and the low dose BYD containing serum (0.284 g/mL, 2 mL) were prepared. The inhibitory effects of different dose and concentrations BYD on the proliferation of A549 and A549/DDP cells were detected by MTT assay, and the drug resistance reversal fold was calculated. The expression of Survivin in the two cell strains were detected respectively by immunohistochemical assay, Western blot, and immunofluorescence method.</p><p><b>RESULTS</b>BYD containing serum showed obvious inhibitory effect on the growth of A549 and 549/DDP. The inhibition rates of 10% dose groups were higher than those of 5% dose groups. Besides, it gradually increased along with increased concentrations. Compared with 10% blank control group, the inhibition rate increased in 10% middle and low dose groups (P <0.05). After acted with 10% middle dose BYD containing serum, IC50, of A549 and A549/DDP were both reduced (P <0.05), reversal fold (RF) both increased. Its reversal ratio on A549/DDP cells was 2. 46, decreasing the resistance of A549/DDP to DDP. Compared with A549 in the same group, the expression of Survivin was detected to decrease by immunocytochemical assay, Western blot, and immunofluorescence method (P<0.05). Compared with 10% blank control group, the inhibition rate decreased in 10% middle dose group (P <0. 05).</p><p><b>CONCLUSIONS</b>10% middle dose BYD containing serum could significantly inhibit the apoptosis of A549 and A549/DDP. Besides, it could moderately reverse the multidrug resistance of A549/DDP cells to DDP possibly through reducing the intracellular expression of Survivin and enhancing the sensitivity 549/DDP to chemotherapeutics.</p>


Subject(s)
Adenocarcinoma , Metabolism , Animals , Apoptosis , Cell Line, Tumor , Cisplatin , Pharmacology , Drug Resistance, Multiple , Drug Resistance, Neoplasm , Drugs, Chinese Herbal , Pharmacology , Inhibitor of Apoptosis Proteins , Metabolism , Lung Neoplasms , Metabolism , Microtubule-Associated Proteins , Pharmacology , Rats
11.
Article in Chinese | WPRIM | ID: wpr-252123

ABSTRACT

<p><b>OBJECTIVE</b>Growth, regeneration and reparation of gastric mucosal epithelium may relate to the expression of peptides. This study aimed to investigate the effect of pS2, TGF-alpha and PCNA in endotoxin-induced acute gastric mucosal injury in young rats.</p><p><b>METHODS</b>Eighteen-day-old Wistar rats were randomly injected intraperitoneally with lipopolysaccharide (LPS) (5 mg/kg) or normal saline (control). The gastric mucosal specimens were harvested 1.5, 3, 6, 24, 48, and 72 hrs after LPS or normal saline injection (n=8 each). The pathological changes of the gastric mucosa were observed by hematoxylin-eosin staining. The expression of pS2,TGF-alpha and PCNA was measured by immunohistochemistry SP method.</p><p><b>RESULTS</b>Gastric mucosal injuries were the most serious 6 hrs after LPS injection, characterized by massive erosion, bleeding and cord necrosis of the gastric mucosa paralleling with gastric longitudinal axis. PCNA expression in the gastric mucosa in the LPS group 3, 6, 24 and 48 hrs after LPS injection was significantly lower than that in the control group (P<0.01). pS2 expression in the gastric mucosa weakened 1.5 hrs after LPS injection, recovered to the control level at 3 hrs and was significantly higher than the control at 6, 24, 48 and 72 hrs of LPS injection (P<0.01). TGF-alpha expression in the gastric mucosa in the LPS group increased significantly 6, 24 and 48 hrs after LPS injection when compared with the control group (P<0.01).</p><p><b>CONCLUSIONS</b>PCNA expression may be associated with the proliferation activity of the gastric mucosa in the process of gastric mucosal injury/reparation. pS2 and TGF-alpha might participate in the defense and reparation of gastric mucosal cells through mediating cell proliferation following acute gastric mucosal injury.</p>


Subject(s)
Animals , Endotoxemia , Metabolism , Female , Gastric Mucosa , Chemistry , Pathology , Immunohistochemistry , Male , Peptides , Proliferating Cell Nuclear Antigen , Rats , Rats, Wistar , Transforming Growth Factor alpha , Trefoil Factor-2
12.
National Journal of Andrology ; (12): 531-534, 2007.
Article in Chinese | WPRIM | ID: wpr-297689

ABSTRACT

<p><b>OBJECTIVE</b>To study the correlation between the severity of psychic symptoms and that of clinical ones in CP patients.</p><p><b>METHODS</b>We investigated 300 CP patients with NIH Chronic Prostatitis Symptom Index (CPSI), Symptom Checklist 90 (SCL-90) and a self-designed questionnaire, and analyzed the correlation between the scores. Based on the scores on pain and urinary symptoms in CPSI, the patients were divided into a mild, a moderate and a severe group.</p><p><b>RESULTS</b>A total of 288 questionnaires were collected. Compared with normal males, the SCL-90 scores of the CP patients were not different in obsessive-compulsive (P > 0.05), lower in interpersonal-sensitivity (P < 0.01), higher in paranoid ideation (P < 0.05) and significantly higher in other factors and the number of positive factors (P < 0.01). There were no statistical differences among the mild, moderate and severe groups either in somatization and interpersonal-sensitivity (P > 0.05) or in phobic anxiety (P > 0.05), but there were between the mild and moderate groups in phobic anxiety (P < 0.05) and significantly among the three groups in other factors and the number of positive factors (P < 0.01 or P < 0.05). No correlation was found between CPSI and SCL-90 in the total scores.</p><p><b>CONCLUSION</b>There was a close correlation between the severity of psychic symptoms and that of clinical symptoms in CP patients. Psychotherapy, especially psychological intervention, plays an important role in the early stage of the disease.</p>


Subject(s)
Adult , Chronic Disease , Humans , Male , Middle Aged , Prostatitis , Psychology , Psychiatric Status Rating Scales
13.
Article in Chinese | WPRIM | ID: wpr-262790

ABSTRACT

<p><b>OBJECTIVE</b>Gastrointestinal dysfunction is closely correlated with the destruction of intestinal barrier function induced by serious infection. Platelet activating factor (PAF) may induce intestinal injuries. This study aimed to investigate the effect of PAF on the injury of intestinal mucosal immuno-barrier function in young rats.</p><p><b>METHODS</b>Eighteen-day-old Wistar rats were randomized to lipopolysaccharide (LPS) (5 mg/kg), LPS plus PAF receptor antagonist and normal saline injection (Control). PAF receptor antagonist BN52021 5 mg/kg was administered before or 30 minutes after LPS injection (pretreatment or treatment). The ileum specimens (n=8) were harvested at 1.5, 3, 6, 24, 48 and 72 hrs after LPS injection. Double antibody-PEG radioimmunoassay was used to determine the secretory IgA (sIgA) content in intestinal mucosa. Hematoxylin and erosin staining was used for histological evaluation. The ratio of wet and dry weight (W/D) of ileum tissues was calculated.</p><p><b>RESULTS</b>Intestinal villi edema, capillary congestion, extension of the subepithelial lympho channel, and polymorphonuclear infiltration in enteric cavity were noted in the LPS group at 1.5, 3, 6 and 24 hrs after LPS injection. In the PAF receptor antagonist group only villi edema was found. The W/D ratio in the LPS group was significantly higher than that in the Control group at all time points, but it was slightly reduced by the PAF receptor antagonist pretreatment or treatment. The sIgA content was obviously decreased after 1.5, 3, 6, 24 and 48 hrs of LPS challenge compared with that in the Control group (P < 0.01). It reached to a nadir at 6 hrs (0.15 +/- 0.04 microg/mL). The level of sIgA in the PAF receptor antagonist group was higher than that in the LPS group at each time point. There was no statistical difference in the sIgA level between the PAF receptor pretreatment and treatment groups.</p><p><b>CONCLUSIONS</b>PAF plays roles in the injury of intestinal immuno-barrier function. Preventive and remedial use of PAF receptor antagonist BN52021 may relieve intestinal injury.</p>


Subject(s)
Animals , Diterpenes , Pharmacology , Ginkgolides , Immunoglobulin A, Secretory , Intestines , Allergy and Immunology , Pathology , Lactones , Pharmacology , Lipopolysaccharides , Toxicity , Platelet Activating Factor , Physiology , Rats , Rats, Wistar
14.
Article in Chinese | WPRIM | ID: wpr-683524

ABSTRACT

Objective To investigate the changes in the glutamate transporter EEAC1 mRNA expression and the injured nerve cell apoptosis after rat cerebral ischemia/reperfusion injury is treated with mild hypothermia. Methods The middle cerebral arteries(MCA)of Sprague-Dawley rats were occluded for 30 minutes,and reper- fused for 90 minutes.Using DIG-labeled CRNA probe and TUNEL,the positive rate of glutamate transporter EE- AC1 mRNA expression and apoptotic cell rate were determined in the sham-operated group,the control group and the mild hypothermia group,respectively.Results The positive rate of EEAC1 mRNA expression and apoptotic cell rate were significantly lower in sham-operated group than those in the control group(P

15.
Microbiology ; (12)1992.
Article in Chinese | WPRIM | ID: wpr-685478

ABSTRACT

?-arbutin is biosynthesized by whole cell method with Xanthomona maltophilia BT-112.The conditions for cell biosynthesized ?-arbutin are investigated as follows:temperature,25℃;concentration of hydroquinone,30mmol/L;mol ratio of sucrose and hydroquinone,20∶1;time course of ?-arbutin biosynthesis,45 hours;rotational speed,160r/min;concentration of Xanthomona maltophilia BT-112,85g/L;concentration of K-2HPO-4-KH-2PO-4 buffer solution,25mmol/L;pH of K-2HPO-4-KH-2PO-4 buffer solution,8.0.Under the above optimal conditions,the maximum of molar conversion yield based on the amount of hydroquinone supplied reaches 86.7%.

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