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1.
Article in Chinese | WPRIM | ID: wpr-862702

ABSTRACT

<b>Objective::To investigate the feasibility of near-infrared spectroscopy for detecting the coating film thickness of Tianshu tablets. <b>Method::Nine batches of Tianshu tablets were taken during the coating process. Then, their near-infrared diffuse reflection spectra were collected. The sample set was divided into calibration set and validation set by Kennard-Stone algorithm. The preprocessing method was selected. The synergy interval partial least squares (siPLS) and moving window partial least squares (mwPLS) were employed to screen the optimal spectral interval. And the corresponding quantitative calibration model of partial least squares (PLS) were established. Some evaluation parameters were adopted to assess the performance of the model. <b>Result::The method of first derivative and Norris Derivative smoothing combined with standard normal variate transformation was suitable for processing the spectra. The optimal PLS model was established in the preferred band interval of siPLS. The correlation coefficient between the predicted value and the measured value of calibration set was 0.966, and the correlation coefficient between the predicted value and the measured value of validation set was 0.991.Both root mean square error of calibration (RMSEC) and root mean square error of prediction (RMSEP) values were small, which showed the fitness and predictive performance of the model were favorable. <b>Conclusion::The near-infrared spectroscopy technique can be used to determine coating film thickness of Tianshu tablets with high accuracy, which provides technical supports for the in-line determination of coating thickness in the production process of traditional Chinese medicine tablets.

2.
Article in Chinese | WPRIM | ID: wpr-850737

ABSTRACT

Objective: To investigate how to rapidly predict coating terminal point in the thin film coating of Tianshu Tablets by near-infrared spectroscopy (NIRS). Methods: Firstly, synergy interval partial least square (siPLS) was used to optimize the modeling intervals and the pretreatment methods were screened. Secondly, according to the optimal modeling parameters, the conformity test and similarity matching were used to establish calibration models based on the established reference spectra library. Subsequently, by comparing the similarities between the test spectra and the reference spectra, the threshold value was set. The relation among the similarities, threshold value and the coating end point was obtained. Finally, the performance of the calibration model was verified by the validation set. Results: During the coating process, the similarities between the test spectra and the reference spectra were gradually increasing until the similarities of continuous test spectra exceeded the threshold, thus, indicating the end point. Conclusion: Conformity test and similarity matching can sensitively monitor the change of similarities among spectra and the corresponding trend. Both models have high performance, which can accurately predict the coating terminal point. It’s of great significance to reduce batch-to-batch variation in film coating and the loss of coating materials, and improve the coating efficiency and the quality uniformity of solid preparation.

3.
Article in Chinese | WPRIM | ID: wpr-840862

ABSTRACT

Hepatic ischemia reperfusion injury (IRI) is a critical problem of liver surgery, especially when comes to liver transplantation. Presently, there are no effective measures for diagnosis, prevention and therapy of IRI, as the mechanisms of IRI still remain unclear. This review summarizes several new hepatic ischemia-reperfusion markers related to cell signal transduction pathway, including transcription factor STAT, HIF-1 and PPARs, transmission factor MAPK, membrane receptor TLR4 and PARs, and iNOS. Animal studies have indicated that IRI was ameliorated by activating or blockading these markers, which might serve as targets for diagnosis, prevention and therapy of IRI.

4.
Article in Chinese | WPRIM | ID: wpr-840525

ABSTRACT

Objective: To observe the expression of local angiotensin-converting enzyme 2 (ACE-2) in rat liver and its relationship with preservation injury (PI) after transplantation, so as to determine the possible role of local renin-angiotensin system (RAS) in PI caused by the cold-preservation in liver transplantation. Methods: The donor liver grafts were divided into cold preservation (CP) and non-cold preservation (NCP) groups; we also included a sham operation group without transplantation. The mRNA and protein levels of ACE-2 in the transplanted livers were detected by quantitative real-time PCR and Western blotting assay, respectively. The location of ACE-2 protein and pimonidazole in liver was detected by immunohistochemistry. Furthermore, histological evaluation was applied to verify the extent of liver damage. Results: Histological injuries of different degrees existed after liver transplantation; the injury was more obvious in the CP group. Compared with the sham operation group, the mRNA and protein expression of ACE-2 was significantly increased after liver transplantation (P<0.05 or P<0.01), and the expression of the CP group was significantly higher than that of the NCP group (P<0.05). Pimonidazole staining was positive around the hepatic veins in the transplantation groups, and the intensity of staining was positively correlated with the expression of ACE-2 mRNA (r=0. 78, P<0.001). Conclusion: It is indicated that ACE-2 expression is closely associated with tissue hypoxia during cold preservation-induced PI, and local RAS may play an important role in PI during liver transplantation.

5.
Article in Chinese | WPRIM | ID: wpr-680421

ABSTRACT

Hepatic ischemia-reperfusion injury(IRI)is a critical problem of liver surgery,especially when comes to liver transplantation.Presently.there are no effective measures for diagnosis,prevention and therapy of IRI,as the mechanisms of IRI still remain unclear.This review summarizes several new hepatic ischemia-reperfusion markers related to cell signal transduetion pathway.including transcription factor STAT,HIF-1 and PPARs,transmission factor MAPK,membrane receptor TLR4 and PARs.and iNOS.Animal studies have indicated that IRI was ameliorated by activating or blockading these markers,which might serve as targets for diagnosis,prevention and therapy of IRI.

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