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1.
Article in English | WPRIM | ID: wpr-880618

ABSTRACT

OBJECTIVES@#Chondrocyte apoptosis is an important process in the pathogenesis of osteoarthritis. Mangiferin exerts multiple pharmacological effects such as anti-inflammatory and anti-apoptosis. However, the role of mangiferin in chondrocyte apoptosis is not clear. In this study, we aimed to explore the role of mangiferin in IL-1β-induced chondrocyte apoptosis.@*METHODS@#ATDC5 cells were randomly divided into a control group, a IL-1β group, a MFN-L group, a MFN-M group, a MFN-H group and a MFN+LY294002 group. Cells in the control group were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN-L group, the MFN-M group and the MFN-H group were pretreated with 5, 10 and 20 μmol/L mangiferin for 1 h respectively, and then they were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN+LY294002 group were treated with LY294002 (25 μmol/L) for 1 h, then mangiferin (20 μmol/L) and IL-1β (10 ng/mL) for 1 h and 24 h, respectively. Cell viability was detected by CCK-8 assay and cell apoptosis was measured by flow cytometry. Colorimetric assay was conducted to measure the caspase-3 activity. The protein levels of Bcl-2, Bax, and phosphoinositide 3-kinase (PI3K)/Akt signaling pathway related proteins were detected by Western blotting.@*RESULTS@#Compared to the control group, cell viability was significantly decreased; cell apoptosis, caspase-3 activity and Bax protein expression were significantly increased; the protein levels of Bcl-2, p-PI3K, and p-Akt were significantly decreased in the IL-1β group (all @*CONCLUSIONS@#Mangiferin could attenuate IL-1β-induced apoptosis of the mice chondrocytes, which is mediated by the activation of PI3K/Akt signaling pathway.


Subject(s)
Animals , Apoptosis , Chondrocytes , Interleukin-1beta , Mice , Phosphatidylinositol 3-Kinases , Proto-Oncogene Proteins c-akt , Xanthones
2.
Experimental Neurobiology ; : 138-149, 2020.
Article | WPRIM | ID: wpr-832439

ABSTRACT

Neuropsychiatric disorders are the leading cause of mental and intellectual disabilities worldwide. Current therapies against neuropsychiatric disorders are very limited, and very little is known about the onset and development of these diseases, and their most effective treatments. MIR137 has been previously identified as a risk gene for the etiology of schizophrenia, bipolar disorder, and autism spectrum disorder. Here we generated a forebrain-specific MIR137 knockout mouse model, and provided evidence that loss of miR-137 resulted in impaired homeostasis of potassium in mouse hippocampal neurons. KCC2, a potassium-chloride co-transporter, was a direct downstream target of miR-137. The KCC2 specific antagonist VU0240551 could balance the current of potassium in miR-137 knockout neurons, and knockdown of KCC2 could ameliorate anxiety-like behavior in MIR137 cKO mice. These data suggest that KCC2 antagonists or knockdown might be beneficial to neuropsychiatric disorders due to the deficiency of miR-137.

3.
Article in Chinese | WPRIM | ID: wpr-876378

ABSTRACT

Objective To explore associations between short-term exposure to ambient nitrogen dioxide (NO2) and hospital outpatient visits. Methods Daily outpatient visit data were obtained from 5 hospitals in Beijing, Shanghai, and Guangzhou, the three major cities in China.The study period was from 2013 to 2015.The causes of disease were determined in accordance to the International Classification of Disease 10th version, with the codes A00-R99.Daily air pollution data were from the nearest state-controlled environmental monitoring stations.City-level environmental factors were acquired, including temperature and relative humidity.A time-series generalized additive model (GAM) was adopted to explore the effect of daily NO2 exposure on hospital outpatient visits, after controlling the time trends, day of week (DOW), holiday, and environmental factors.Then a random-effect meta-analysis was used to pool the results across cities.We also explored the lag structure, two-pollutant models, and potential seasonal effect. Results During the study period, the average daily outpatient visits were 3 949.The average NO2 exposure level was 55.3 μg/m3.The lag analysis showed that NO2 exposure had the greatest effect on outpatient visits every day. For a 10 μg/m3 increase in NO2, outpatient visits remarkably increased by 0.85% (95%CI:0.51%-1.19%).The associations of NO2 remained robust after controlling co-pollutants, including fine particulate matter, ozone, sulfur dioxide and carbon monoxide.The effects of NO2 were more in cool seasons, and the exposure-response curves between NO2 and outpatient visits were nearly linear. Conclusions Short-term exposure to NO2 increases the risk of hospital outpatient visits in major cities, suggesting a need to further lower exposure levels of NO2 in China.

4.
Article in Chinese | WPRIM | ID: wpr-798963

ABSTRACT

Objective@#To evaluate the effect of aminolevulinic acid-based photodynamic therapy (ALA-PDT) on the expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) in condyloma acuminatum (CA) tissues.@*Methods@#A total of 56 patients with CA were enrolled from Department of Dermatology, The First Affiliated Hospital of Zhengzhou University from October 2016 to September 2017, and skin lesions were obtained before and 1 week after the first ALA-PDT treatment. Immunohistochemical SP method was used to determine the expression of VEGF and PCNA in keratinocytes in the CA tissues. Chi-square test and rank sum test were carried out to analyze differences between pre- and post-treatment expression rate and intensity of VEGF and PCNA, and Spearman correlation analysis was conducted to analyze the correlations between the protein expression of VEGF and PCNA.@*Results@#The expression rates of VEGF and PCNA in keratinocytes in the CA tissues were 71.43% (40/56) and 73.21% (41/56) respectively before ALA-PDT, and 44.64% (25/56) and 41.07% (23/46) respectively after ALA-PDT. There were significant differences between pre- and post-treatment expression rate and intensity of VEGF and PCNA (expression rate: χ2 = 8.25, 11.81 respectively, both P < 0.05; expression intensity: H = 11.29, 12.22 respectively, both P < 0.05) . The expression of VEGF was positively correlated with the expression of PCNA in the CA tissues before and after the ALA-PDT treatment (rs = 0.202, 0.273, respectively, both P < 0.05) .@*Conclusion@#The expression of VEGF and PCNA decreased in CA tissues after ALA-PDT treatment, which may be one of the mechanisms underlying the treatment of CA with ALA-PDT.

5.
Article in Chinese | WPRIM | ID: wpr-798726

ABSTRACT

The interventional treatment of congenital heart diseases has been developed for more than 50 years since Rashkind et al.performed balloon atrial septostomy in 1966.From the initial catheter examination and palliative operation to the current radical occlusion, stent implantation, valve replacement, etc, interventional catheterization has become one of main means of treating congenital heart disease.The interventional therapy of congenital heart disease in China has made great progress, but there is still a certain gap with developed countries.This article summarizes the progress of interventional treatments in septal defect diseases, aortic artery to pulmonary artery shunt diseases, valvular diseases, and hybrid therapy, in order to provide reference for the interventional treatment of congenital heart disease in China.

6.
Article in Chinese | WPRIM | ID: wpr-798724

ABSTRACT

Implantable cardioverter defibrillator (ICD) plays an irreplaceable role in preventing sudden cardiac death.There has been a continuous increase in the implantation rate of ICD in children along with technologic improvement.However, the choice of indication, device and implantation approaches of ICD still faces great challenges due to the particularity of children.Related issues on the clinical application of ICD in children, including common diseases, indications and the clinical application of ICD, are reviewed in this paper.

7.
Article in Chinese | WPRIM | ID: wpr-828664

ABSTRACT

OBJECTIVE@#To study the biomarkers for human coronary artery endothelial cell (HCAEC) injury induced by Kawasaki disease (KD) using isobaric tags for relative and absolute quantitation (iTRAQ) proteomics.@*METHODS@#HCAECs cultured with the serum of children with KD were used as the KD group, and those cultured with the serum of healthy children was used as the healthy control group. The iTRAQ technique was used to measure the expression of proteins in two groups. The data on proteins were analyzed by bioinformatics. Western blot was used for the validation of protein markers.@*RESULTS@#A total of 518 significantly differentially expressed proteins were identified (with an absolute value of difference fold of >1.2, P<0.05). The gene ontology analysis showed that the differentially expressed proteins were significantly enriched in biological processes (including cellular processes, metabolic processes, and biological regulation), cellular components (including cell parts, cells, and organelles), and molecular functions (including binding, catalytic activity, and molecular function regulators). The KEGG analysis showed that the proteins were significantly enriched in the signaling pathways of ribosomes, PI3K-Akt signaling pathway, and transcriptional dysregulation in cancer. The PPI network showed that the top 9 protein markers in relation density were PWP2, MCM4, MCM7, MCM5, MCM3, MCM2, SLD5, HDAC2, and MCM6, which were selected as the protein markers for coronary endothelial injury in KD. Western blot showed that the KD group had significantly lower expression levels of the protein markers HDAC2, PWP2, and MCM2 than the healthy control group (P<0.05).@*CONCLUSIONS@#The serum of children with KD significantly changes the protein expression pattern of HCAECs and affects the signaling pathways associated with the cardiovascular system, which provides a new basis for the pathophysiological mechanism and therapeutic targets of KD.


Subject(s)
Child , Computational Biology , Coronary Vessels , Endothelial Cells , Humans , Mucocutaneous Lymph Node Syndrome , Phosphatidylinositol 3-Kinases , Proteomics
8.
Article in Chinese | WPRIM | ID: wpr-827811

ABSTRACT

To investigate the effects of exogenous NaHS on myelin basic protein (MBP) and learning and memory of hippocampal neurons in mice with spinocerebellar ataxia type 3 (SCA3) and its therapeutic significance. Twelve male normal mice were randomly selected as normal control group (NC Group), and 48 SCA3 mice were randomly selected as SCA3 model group (M Group), low dose group (NL Group, 10 μmol/kg), medium dose group (NM Group, 50μmol/kg) and high dose group (NH Group, 100 μmol/kg), 12 rats in each group. The drug treated groups were injected with NaHS intraperitoneally once a day for 4 weeks. The changes of learning and memory ability of SCA3 mice before and after the intervention of different doses of NaHS were determined by Morris water maze, the content of hydrogen sulfide (HS) in hippocampus was measured by spectrophotometry, the expression of MBP was detected by immunohistochemistry, and the morphological changes of neuron myelin sheath were observed by electron microscope. Compared with the control group, the learning and memory ability of SCA3 mice was decreased significantly (P<0.05), and the content of HS in hippocampus was decreased (P<0.05). After different doses of exogenous NaHS treatment, the learning and memory ability was improved in different degrees (P<0.05), and the contents of HS and MBP in hippocampus of SCA3 mice were also improved in different degrees (P<0.05). Exogenous NaHS may increase the contents of HS and MBP in the hippocampus of SCA3 mice, which may have a protective effect on the neurons, and then improve the learning and memory ability of SCA3 mice, and provide a new idea for the treatment of SCA3.

9.
Article in Chinese | WPRIM | ID: wpr-873331

ABSTRACT

Objective::To clone p-coumaroyl quinate/shikimate 3' -hydroxylase gene from Lonicera macranthoides, and analyze its bioinformatics and expression patterns with chlorogenic acid content, in order to speculate the functions of LmC3H1 gene from L. macranthoides. Method::The full-length cDNA sequence of LmC3H1 gene was cloned by reverse trascription polymerase chain reaction(RT-PCR) and RACE techniques. The bioinformatics analysis of the gene sequence was carried out by using relevant software.Real-time fluorescence quantification PCR(Real-time PCR) and HPLC were used to determine relative expression of LmC3H1 and content of chlorogenic acid in stems, leaves and flowers of different flowering stages. Result::The LmC3H1 (GenBank: MN177695) gene was cloned, and the open reading frame (ORF) of it was 1 533 bp in length and encoded 510 amino acids. The molecular formula was C2618H4134N718O727S22, the relative molecular mass was 58 005.32, and the isoelectric point was 8.92.It was a hydrophilic protein located in the chloroplast with a transmembrane region LLLIPAVLFLISLVYPLI, and contained a conserved domain CYTOCHROME_P450(433-422 aa) in cytochrome P450.The results of Real-time PCR showed that LmC3H1 was expressed in different degrees in stems, leaves and different flowering stages of L. macranthoides. In the flower development stage, the relative expression of white bud stage was the highest, followed by flower buds and white flowering stage. The ratio of flower to stem and leaf was the highest, and the relative expression of flower was the highest. The HPLC results showed that the content of chlorogenic acid increased from greenish white to golden yellow in flowering stage and golden yellow flowering stage. Among the different organs, the flower had the highest chlorogenic acid, and the stem showed the lowest. Conclusion::The LmC3H1 gene of L. macranthoides is cloned, suggesting that LmC3H1 might be involved in the biosynthesis of L. macranthoides chlorogenic acid. This study provides a basis for further studying the functions of the gene and exploring the biosynthesis and regulation mechanism of L. macranthoides chlorogenic acid, while laying the foundation for the genetic improvement of L. macranthoides.

10.
Article in Chinese | WPRIM | ID: wpr-873256

ABSTRACT

Objective::To study on the content change and transformation rule of eight main characteristic components in stir-frying process of Glycyrrhizae Radix et Rhizoma. Method::The contents of liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, isoliquiritigenin, glycyrrhizic acid and glycyrrhetinic acid in Glycyrrhizae Radix et Rhizoma were determined simultaneously by HPLC. The chromatographic conditions were Waters Symmetry® C18 column (4.6 mm×250 mm, 5 μm), and the mobile phase of acetonitrile (A)-0.05%phosphoric acid solution (B) for gradient elution (0-9 min, 19%-25%A; 9-18 min, 25%-34%A; 18-38 min, 34%-51%A; 38-58 min, 51%-89%A), the flow rate of 1 mL·min-1, the detection wavelengths at 320 nm (0-16 min), 276 nm (16-25 min), 370 nm (25-28 min), 254 nm (28-58 min), the injection volume of 10 μL and the column temperature at 30 ℃. Result::After stir-frying, the total content of three components with dihydroflavone as mother nucleus was decreased, while the total content of three components with chalcone as mother nucleus showed an upward trend, the content change of glycyrrhizic acid was not obvious, but glycyrrhetinic acid content showed a slight upward trend. When the monomer heating temperature reached 130 ℃, dihydroflavones and chalcones could be isomerized with each other, and with the increase of temperature, the isomerization became more obvious. When the heating temperature rose to 180 ℃ (isoliquiritin apioside was 130 ℃), in addition to the isomerization, the glucosidic bond of flavonoid glycosides began to break and gradually transformed into the corresponding secondary glycosides or aglycones. Glucosidic bond of glycyrrhizic acid could also be broken to form glycyrrhetinic acid, which was detected at 150 ℃. Conclusion::The change of chemical composition is complex during stir-frying process of Glycyrrhizae Radix et Rhizoma, in addition to the isomerization and glucosidic bonds breaking observed in this experiment, there may be other complex reactions. The content of one compound in the herb is affected by many factors during its processing, such as the time and temperature of frying, the stability of the compound itself and so on.

11.
Cancer Research and Clinic ; (6): 781-785, 2020.
Article in Chinese | WPRIM | ID: wpr-872587

ABSTRACT

Objective:To explore the curative effect and safety of unilateral radical thyroidectomy via axillary approach endoscope without air insufflation.Methods:The clinical data of 80 patients with unilateral thyroid cancer in Huaibei People's Hospital in Anhui Province from June 2017 to June 2019 were retrospectively analyzed. The patients were divided into the control group (40 cases) and the observation group (40 cases) according to the surgical method. The observation group was treated with unilateral radical thyroidectomy via axillary approach endoscope without air insufflation, and the control group was treated with open surgery plan. The dissection number of lymph nodes in the central area, the time of flap separation and cavity establishment, the total operation time, the drainage volume and the days of hospital stay in patients of two groups were recorded, and the postoperative complications were evaluated and recorded. The neck and chest pain of patients were investigated with visual analogue scoring method (VAS) at 3 days, 1 month and 3 months after surgery. The white blood cell count (WBC), interleukin 6 (IL-6) and C-reactive protein (CRP) levels were measured before and 24 hours after treatment. The cosmetic satisfaction of the patients was investigated at 3 months after surgery.Results:The time of flap separation and cavity construction, total operation time, drainage volume and hospitalization days in the observation group were (34.27±4.38) min, (130.75±12.28) min, (143.49±15.48) ml, and (7.21±1.95) days, and they were (12.43±1.83) min, (90.38±8.65) min, (87.48±12.11) ml, and (5.48±1.32) days in the control group, and the differences between the two groups were statistically significant ( t values were 29.098, 17.002, 18.024, and 3.576, all P < 0.01). The VAS score of chest pain in the observation group was higher than that in the control group at 3 days after surgery ( P < 0.05); the CRP and WBC levels in the observation group were lower than those in the control group at 24 hours after treatment (both P < 0.05). The cosmetic satisfaction rating grade in the observation group was 6 cases of grade Ⅰ, 31 cases of grade Ⅱ, 2 cases of grade Ⅲ and 1 case of grade Ⅳ, and there were 1 case of grade Ⅰ, 10 cases of grade Ⅱ, 24 cases of grade Ⅲ and 5 cases of grade Ⅳ in the control group, the cosmetic satisfaction of the observation group was better than that of the control group ( P < 0.05). There was no significant difference in the incidence of postoperative wound infection, temporary recurrent laryngeal nerve paralysis and subcutaneous hematoma between the two groups (all P > 0.05). Conclusion:The radical thyroidectomy via axillary approach endoscope without air insufflation has good therapeutic and cosmetic effects, and it can effectively reduce the inflammatory response in patients.

12.
Article in Chinese | WPRIM | ID: wpr-870217

ABSTRACT

Objective To evaluate the effect of aminolevulinic acid-based photodynamic therapy (ALA-PDT) on the expression of vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) in condyloma acuminatum (CA) tissues.Methods A total of 56 patients with CA were enrolled from Department of Dermatology,The First Affiliated Hospital of Zhengzhou University from October 2016 to September 2017,and skin lesions were obtained before and 1 week after the first ALA-PDT treatment.Immunohistochemical SP method was used to determine the expression of VEGF and PCNA in keratinocytes in the CA tissues.Chi-square test and rank sum test were carried out to analyze differences between pre-and post-treatment expression rate and intensity of VEGF and PCNA,and Spearman correlation analysis was conducted to analyze the correlations between the protein expression of VEGF and PCNA.Results The expression rates of VEGF and PCNA in keratinocytes in the CA tissues were 71.43% (40/56) and 73.21% (41/56) respectively before ALA-PDT,and 44.64% (25/56) and 41.07% (23/46)respectively after ALA-PDT.There were significant differences between pre-and post-treatment expression rate and intensity of VEGF and PCNA (expression rate:x2 =8.25,11.81 respectively,both P < 0.05;expression intensity:H =11.29,12.22 respectively,both P < 0.05).The expression of VEGF was positively correlated with the expression of PCNA in the CA tissues before and after the ALA-PDT treatment (rs =0.202,0.273,respectively,both P < 0.05).Conclusion The expression of VEGF and PCNA decreased in CA tissues after ALA-PDT treatment,which may be one of the mechanisms underlying the treatment of CA with ALA-PDT.

13.
Article in Chinese | WPRIM | ID: wpr-868492

ABSTRACT

Objective:To screen the key miRNA downstream of TLR2 and explore the function of the miR-21.Methods:Wild type (WT) and TLR2 KO mice were irradiated with 60Co γ-ray to compare their survivals. The downstream miRNAs of TLR2 signaling pathway were screened by RNA sequence in BMCs, and their expressions were verified by QT-PCR. Cell lines with overexpression or knockdown of a miRNA were established to evaluate the function of miRNA. Results:The radiosensitivity of TLR2 KO mice was higher than that of TLR2 WT mice( χ2=4.490, 13.100, 7.928, P<0.05). The bone marrow transplantation experiment proved that the increased radiosensitivity of TLR2 KO mice was related to BMCs ( χ2=4.291, P<0.05). A total of 55 differentially expressed genes were screened by RNA sequence ([log2 Fold Change]>0.95, Q<0.05), of which 28 were up-regulated and 27 were down-regulated. QT-PCR assay determined that miR-21 was down-regulated in BMCs of TLR2 KO ( t=9.420, P<0.01) and MyD88 KO ( t=10.700, P<0.01) mice. It was proved by QT-PCR that the expressions of IL-6 ( t=13.790, P<0.05) and TNF-α ( t=14.280, P<0.05) were increased in a TLR2 dependent manner after PAM3CSK4 stimulation. Overexpression of miR-21 promoted viability of EL4 cells ( t=5.951, P<0.05) and NIH/3T3 cells ( t=4.786, P<0.05) and reduced BMCs apoptosis in WT ( t=4.842, P<0.05) and TLR2 KO ( t=10.520, P<0.05) mice after radiation. Inhibition of miR-21 decreased the viability of EL4 cells ( t=4.815, P<0.05) and NIH/3T3 cells ( t=4.042, P<0.05). Conclusions:miR-21 plays a key regulatory role in the process of TLR2 radioprotection, which may be related to the up-regulation of IL-6 and TNF-α.

14.
Journal of Chinese Physician ; (12): 1445-1448, 2020.
Article in Chinese | WPRIM | ID: wpr-867416

ABSTRACT

Chronic obstructive pulmonary disease (COPD) complicated with pulmonary tuberculosis (PTB) is becoming more and more common. The coexistence of two diseases brings difficulty to the control of the disease. Smoking, biofuel exposure, vitamin D deficiency and diabetes may be common risk factors for both diseases. Complementary prevention and control strategies can be designed for the above risk factors. This article contains review with tuberculosis as a risk factor for COPD, COPD combined with tuberculosis, and the prevention strategies for both diseases.

15.
Journal of Chinese Physician ; (12): 1168-1171, 2020.
Article in Chinese | WPRIM | ID: wpr-867367

ABSTRACT

Objective:To investigate the relationship between neutrophil / lymphocyte ratio (NLR) and cystatin C (Cys-C) and microvascular complications in type 2 diabetes mellitus.Methods:1 990 patients with type 2 diabetes mellitus were divided into simple diabetes group (N group), simple diabetic retinopathy group (DR group), simple diabetic nephropathy group (DN group) and diabetic retinopathy combined with diabetic nephropathy group (DRN group). The general data and biochemical indexes were collected to analyze the differences of NLR and Cys-C among the groups. The risk factors of diabetic microvascular complications were analyzed by multivariate logistic regression.Results:The NLR in DRN group was higher than that in N group and DN group ( P<0.05), but there was no difference between DRN group and DR group ( P>0.05). The serum Cys-C in DRN group was higher than that in N group, DN group and DR group, with statistical significance ( P<0.05). After adjusting for age, duration of diabetes and other factors, multivariate logistic regression showed that NLR was an independent risk factor for type 2 diabetic retinopathy complicated with diabetic nephropathy, while serum Cys-C was not. Subgroup analysis showed that NLR was an independent risk factor for both diabetic retinopathy and diabetic nephropathy, while Cys-C was only related to diabetic nephropathy, which was not related to retinopathy ( P<0.05). Conclusions:NLR is associated with type 2 diabetic retinopathy and diabetic nephropathy, while serum Cys-C is only associated with type 2 diabetic nephropathy, but not with diabetic retinopathy.

16.
Article in Chinese | WPRIM | ID: wpr-866015

ABSTRACT

The US naval continuing medical education is in a leading position all over the world, and to study the US naval continuing medical education plays an important reference role in the development of naval continuing medical education in China. This article expounds the characteristics, organization setting, curriculum setting, medical training and independent escort training of the US naval continuing medical education, and analyzes its enlightenments to the naval continuing medical education of China. We need to learn from the experience of the US naval continuing medical education, combined with the actual characteristics of our navy, improve the training system of continuing medical education, adopt diversified educational technologies, create a scientific education management system, and optimize curriculum reform, thus constantly improving the quality and effectiveness of naval continuing medical education in China.

17.
Article in Chinese | WPRIM | ID: wpr-864037

ABSTRACT

Kawasaki disease (KD) is one of the acute vasculitis diseases, and its most common complications are coronary artery lesions and myocarditis.Different degrees of myocardial injury can be observed in the acute phase of KD, and different abnormal waveforms can be detected in the electrocardiogram (ECG). As a noninvasive electrophysiological examination, ECG is of great significance for both early diagnosis and prognosis evaluation of KD.The advance in research enables ECG manifestations and electrophysiological significance in the early phase of KD to be gradually re-cognized.In this article, the foreign and domestic research on the changes and clinical significance of ECG in acute stage of Kawasaki disease in recent years was reviewed and summarized, and recent advance in the application of ECG in the acute phase of KD was also summarized.

18.
Article in Chinese | WPRIM | ID: wpr-864031

ABSTRACT

Objective:To investigate the histone acetylation of interleukin-4(IL-4) gene and its roles in immunological pathogenesis of Kawasaki disease (KD).Methods:Thirty-six children with KD and 28 age-matched healthy children in Shenzhen Children′s Hospital from October 2016 to December 2018 were recruited in this study.Peripheral venous blood samples were collected from healthy controls (28 cases) and patients with KD during acute phase and 4 to 5 days after effective intravenous immunoglobulin (IVIG) treatment.Co-immunoprecipitation followed by real-time PCR was used to assess histone H4 acetylation levels of IL-4 promoter and Va enhancer, and binding abilities of p300 and CREB-binding protein (CBP) with promoter and Va enhancer of IL-4 gene in peripheral blood CD4 + T cells.Flow cytometry was performed to analyze the proportion of CD4 + IL-4 + T cells (Th2) and protein le-vels of phosphorylated signal transducer and activator of transcription 6 (pSTAT6), GATA binding protein 3 (GATA3), nuclear factor 1 of activated T cells(NFAT1), transforming growth factor-β receptor Ⅱ (TGF-βRⅡ), and phosphorylated L-type amino acid transporter 1(pLAT1). Quantitative real-time PCR was used to evaluate the transcription levels of IL-4, IL-5, IL-13, IL-4 receptor α (IL-4Rα), transforming growth factor-β receptor Ⅰ (TGF-βRⅠ) and sex-determining region Y(SRY)-box 4 (SOX4) in CD4 + T cells.Plasma concentrations of IL-4 and transforming growth factor-β(TGF-β) were measured by enzyme-linked immunosorbent assay. Results:(1)Compared with control group, the proportion of Th2 cells, expression levels of Th2-associated cytokines (IL-4, IL-5 and IL-13) and histone H4 acetylation levels associating with IL-4 promoter and Va enhancer, increased remarkably during acute KD(all P<0.05), and restored after IVIG therapy(all P<0.05). Meanwhile, all the former items in KD patients with coronary artery lesions (CAL) were higher than those in patients with non-coronary artery lesions (NCAL) (all P<0.05). (2) Compared with control group, binding abilities of p300 and CBP with IL-4 promoter and Va enhancer in CD4 + T cells were up-regulated significantly during acute KD (all P<0.05), and decreased in varying degrees after IVIG treatment (all P<0.05). Positive correlations between binding abilities of p300 with IL-4 (promoter and Va enhancer) and the expression of IL-4 promoter and Va enhancer were detected in patients with acute KD ( r=0.72, 0.43, all P<0.05). Furthermore, binding abilities of p300 and CBP with IL-4 promoter and Va enhancer in CAL group were higher than those in NCAL group (all P<0.05). (3) Compared with control group, patients with acute KD had remarkably increased plasma concentration of IL-4, and expression levels of IL-4Rα/STAT6/GATA-3 and pLAT1/NFAT1 in CD4 + T cells (all P<0.05), and significantly down-regulated plasma concentration of TGF-β and expression level of TGF-βRⅡ/TGF-βRⅠ/SOX4 (all P<0.05). All the items mentioned above restored in varying degrees after IVIG treatment (all P<0.05). Simultaneously, the 6 items aforementioned in CAL group were found to be higher than those in NCAL group (all P<0.05), while the latter four items were lower than those in NCAL group (all P<0.05). Conclusion:Histone hyperacetylation of IL-4 gene may be related to immune dysfunction in patients with KD.

19.
Article in Chinese | WPRIM | ID: wpr-863967

ABSTRACT

The interventional treatment of congenital heart diseases has been developed for more than 50 years since Rashkind et al.performed balloon atrial septostomy in 1966.From the initial catheter examination and palliative operation to the current radical occlusion,stent implantation,valve replacement,etc,interventional catheterization has become one of main means of treating congenital heart disease.The interventional therapy of congenital heart disease in China has made great progress,but there is still a certain gap with developed countries.This article summarizes the progress of interventional treatments in septal defect diseases,aortic artery to pulmonary artery shunt diseases,valvular diseases,and hybrid therapy,in order to provide reference for the interventional treatment of congenital heart disease in China.

20.
Article in Chinese | WPRIM | ID: wpr-863965

ABSTRACT

Implantable cardioverter defibrillator (ICD) plays an irreplaceable role in preventing sudden cardiac death.There has been a continuous increase in the implantation rate of ICD in children along with technologic improvement.However,the choice of indication,device and implantation approaches of ICD still faces great challenges due to the particularity of children.Related issues on the clinical application of ICD in children,including common diseases,indications and the clinical application of ICD,are reviewed in this paper.

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