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1.
Article in Chinese | WPRIM | ID: wpr-936070

ABSTRACT

Objective: To summarize short-term postoperative complications of transanal total mesorectal excision (taTME) in the treatment of middle-low rectal cancer. Methods: A descriptive case series of cases was constructed. Clinical data of consecutive 83 patients with mid-low rectal cancer who received taTME treatment from November 2016 to April 2021 at Department of General Surgery of Beijing Friendship Hospital, Capital Medical University were collected. Among 83 patients, 58 (69.9%) were males, with a mean age of (61.4±11.8) years; 42 (50.6%) were low rectal cancer, 41 (49.4%) were middle rectal cancer. Short-term postoperative complication was defined as complication occurring within 30 days after operation. The complication was graded according to the Clavien-Dindo classification. At the same time, the morbidity of short-term postoperative complication in the first 40 patients and that in the last 43 patients were compared to understand the differences before and after passing the taTME learning curve. Results: Two patients (2.5%) were converted to laparotomy ; 78 (94.0%) completed anastomosis.While 5 (6.0%) underwent permanent stoma. The total operation time of transabdominal+ transanal procedure was (246.9±85.0) minutes, and the median intraoperative blood loss was 100 (IQR: 100) ml. Seventy-five cases (75 /78, 96.2%) underwent defunctioning stoma, including 74 cases of diverting ileostomy, 1 case of diverting transverse colostomy and 3 cases without stoma. The morbidity of complication within 30 days after operation was 38.6% (32/83), and the morbidity of complication after discharge was 8.4% (7/83). Minor complications accounted for 31.3% (26/83) and major complications accounted for 7.2% (6/83). No patient died within 30 days after operation. The incidence of anastomotic leakage was 15.4% (12/78). Eight patients (9.6%) were hospitalized again due to complications after discharge. The median postoperative hospital stay was 7 (IQR: 3) days. All the patients with minor (I-II) complications received conservative treatment. One patient with grade C anastomotic leakage was transferred to intensive care unit and received a second operation due to sepsis and multiple organ dysfunction. Two patients with paralytic ileus (Clavien-Dindo IIIa) underwent endoscopic ileus catheter placement. There were 3 patients with Clavien-Dindo III or above respiratory complications, including 1 patient with pleural effusion and ultrasound-guided puncture, 2 patients with respiratory failure who were improved and discharged after anti-infection and symptomatic treatment. One patient underwent emergency ureteral stent implantation due to urinary infection (Clavien-Dindo IIIb). The morbidity of postoperative complication in the first 40 cases was 50.0% (20/40), and that in the latter 43 cases decreased significantly (27.9%, 12/43), whose difference was statistically significant (χ(2)=4.270, P=0.039). Conclusions: The procedure of taTME has an acceptable morbidity of short-term postoperative complication in the treatment of mid-low rectal cancer. The accumulation of surgical experience plays an important role in reducing the morbidity of postoperative complication.


Subject(s)
Aged , Anal Canal/surgery , Anastomotic Leak/etiology , Female , Humans , Male , Middle Aged , Operative Time , Proctectomy/methods , Rectal Neoplasms/surgery
2.
Article in Chinese | WPRIM | ID: wpr-928266

ABSTRACT

OBJECTIVE@#To investigate the effect of tibial coronal curvature on the alignment of tibial prosthesis in patients undergoing total knee arthroplasty (TKA).@*METHODS@#From July 2019 to April 2021, 100 patients with knee osteoarthritis were treated with total knee arthroplasty. Before operation, the full-length films of lower limbs were taken and the tibial bowing angle(TBA) was measured. TBA more than 2° was tibial bending, which was divided into tibial bending group and non bending group. There were 40 cases in tibial bending group, 9 males and 31 females, aged 56 to 84 years old with an average of (69.22±7.10) years. There were 60 cases in the non bending group, 19 males and 41 females, aged from 51 to 87 years old with an average of (70.80±7.21) years. The preoperative tibial length (TL) and medial proximal tibial angle (MPTA) were measured and compared between the two groups. The full-length X-rays of the lower limbs were taken again 3 days to 1 week after operation. The medial angle of the tibial component coronal aligement angle (TCCA) and the outilier rate of force line of the tibial prosthesis were measured and compared between the two groups. Pearson method was used to analyze the correlation between TCCA and age, TCCA and height, TCCA and weight, TCCA and BMI, TCCA and TBA, TCCA and TL, TCCA and MPTA; Spearman method was used to analyze the correlation between TCCA and gender, TCCA and Kellgren-Lawrence(K-L) grade.@*RESULTS@#All 100 patients successfully completed the operation and obtained satisfactory full-length X-rays in standing position. There was no significant difference in TL, MPTA and TCCA between bending group and non bending group(P>0.05). The outilier rate of force line in tibial bending group was 22.5%, and that in non bending group was 6.67%, the difference was statistically significant(P<0.05). The correlation study found that TCCA was strongly correlated with TBA(r=-0.702, P<0.01), weakly correlated with MPTA(r=0.311, P<0.01), and had no correlation with other parameters(P>0.05).@*CONCLUSION@#In patients with knee osteoarthritis undergoing total knee arthroplasty, tibial bending will lead to poor force line of tibial prosthesis. During operation, attention should be paid to osteotomy of proximal tibial vertical tibial mechanical axis and correct installation of prosthesis to avoid poor alignment of prosthesis.


Subject(s)
Aged , Aged, 80 and over , Arthroplasty, Replacement, Knee/adverse effects , Female , Humans , Knee Joint/surgery , Knee Prosthesis/adverse effects , Male , Middle Aged , Osteoarthritis, Knee/surgery , Retrospective Studies , Tibia/surgery
3.
Article in English | WPRIM | ID: wpr-929237

ABSTRACT

Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale, afforded nine citrinin derivatives (1-9) and one peptide-polyketide hybrid GKK1032B (10). The structures of these compounds were determined by spectroscopic methods. The absolute configurations of 1 and 2 were determined for the first time by calculation of electronic circular dichroism (ECD) data. Among them, GKK1032B (10) showed significant cytotoxicity against human osteosarcoma cell line MG63 with an IC50 value of 3.49 μmol·L-1, and a primary mechanistic study revealed that it induced the apoptosis of MG63 cellsvia caspase pathway activation.


Subject(s)
Apoptosis , Bone Neoplasms , Caspases , Humans , Osteosarcoma/drug therapy , Penicillium
4.
Journal of Clinical Hepatology ; (12): 2696-2700, 2021.
Article in Chinese | WPRIM | ID: wpr-905024

ABSTRACT

Acute-on-chronic liver failure (ACLF) is a life-threatening disease with a high risk of multiple organ failure, sepsis, and death. ACLF activates innate and acquired immune responses in human body and thus leads to the progression of persistent systemic inflammatory response syndrome and multiple organ dysfunction, leading to the high mortality rate of this disease. Dysregulated immune response plays a key role in disease progression, and immunotherapy may help to target immune-mediated organ damage and inhibit the progression of liver failure. This article reviews the role and mechanism of drugs and means with a potential immune regulatory effect in ACLF, in order to provide a reference for immunotherapy for ACLF.

5.
Article in Chinese | WPRIM | ID: wpr-879445

ABSTRACT

OBJECTIVE@#To investigate the expression and clinical significance of receptor interacting protein serine-threonine kinases 1 (RIPK1) in the nucleus pulposus of patients with lumbar disc herniation (LDH).@*METHODS@#Nucleus pulposus tissue specimens of 40 patients with LDH patients underwent surgical treatment from January 2016 to January 2018 as the case group, and nucleus pulposus tissue specimens of 30 patients with lumbar spine fracture underwent surgical treatment at the same time as the control group. The expression of RIPK1 mRNA and protein of receptor interaction were detected by polymerase chain reaction (PCR) and Western blot, respectively. The expression of RIPK1 protein in the nucleus pulposus were detected by immunohistochemical staining. The concentrations of RIPK1 and tumor necrosis factor-α (TNF-α) in nucleus pulposus were detected by ELISA method. The relationship between the concentrations of RIPK1, TNF-α in nucleus pulposus and the Pearce grade of LDH patients was analyzed by one-way ANOVA. The correlation between RIPK1 and TNF-α was analyzed by Pearson.@*RESULTS@#RIPK1 was weakly positively expressed in nucleus pulposus of control group, and RIPK1 protein was positively or strongly positively expressed in case group. The expression of RIPK1 mRNA in nucleus pulposus of case group was higher than that of control group (@*CONCLUSION@#The expression levels of RIPK1 mRNA and protein in the intervertebral disc tissues of LDH patients are higher than those of normal intervertebral disc tissues, and increased with the increase of Pearce grade, which may be an important factor involved in LDH inflammatory disease.


Subject(s)
Humans , Intervertebral Disc/metabolism , Intervertebral Disc Degeneration , Intervertebral Disc Displacement/genetics , Nucleus Pulposus , Receptor-Interacting Protein Serine-Threonine Kinases/genetics , Tumor Necrosis Factor-alpha/metabolism
6.
Journal of Clinical Hepatology ; (12): 2375-2379, 2020.
Article in Chinese | WPRIM | ID: wpr-829422

ABSTRACT

At present, hepatic encephalopathy has a relatively high mortality and thus greatly affects patients’ quality of life. This article describes the changes of intestinal flora in patients with hepatic encephalopathy and analyzes the mechanism of action of intestinal flora in hepatic encephalopathy and related treatment methods. It is pointed out that the development of hepatic encephalopathy is closely associated with intestinal flora, and clinical treatment by regulating intestinal flora has achieved a marked effect in patients with hepatic encephalopathy. In the future, the research on intestinal flora in patients with hepatic encephalopathy can be deepened to provide better regimens for the treatment of hepatic encephalopathy.

7.
Journal of Clinical Hepatology ; (12): 1874-1879, 2020.
Article in Chinese | WPRIM | ID: wpr-825048

ABSTRACT

The development and progression of nonalcoholic fatty liver disease (NAFLD) have complex potential mechanisms. The traditional “two-hit” pathophysiological theory has been challenged, and in recent years, an increasing number of studies have been performed to investigate the interaction between insulin resistance, adipokines, and other unknown pathogenic factors in various organs. This article summarizes the factors of the liver, intestinal tract, hypothalamus, and extracellular cysts, as well as genetic factors, with an emphasis on the synergistic mechanism of action of the liver and extrahepatic organs in the pathogenesis of NAFLD, in order to provide a reference for obtaining new insights into NAFLD regulatory network and determining new targets for the prevention and treatment of NAFLD.

8.
Journal of Clinical Hepatology ; (12): 2847-2850, 2020.
Article in Chinese | WPRIM | ID: wpr-837663

ABSTRACT

As a novel form of programmed cell death different from cell necrosis, apoptosis, and autophagy discovered in recent years, pyroptosis is characterized by cell membrane rupture and release of cell contents and proinflammatory factors mediated by gasdermin, thus leading to cell death. Pyroptosis signaling pathways can be classified into classical pathways dependent on caspase-1 and non-classical pathways dependent on caspase-4/5/11; the activation of caspase-1 in classical pathways depends on the function of inflammasome, while the direct activation of caspase-4/5/11 is observed in non-classical pathways, which leads to the lysis of gasdermin D and induce the formation of membrane pores, the maturation and release of interleukin-1β and interleukin-18, and the rupture of cell membrane to cause pyroptosis. Latest research has shown that pyroptosis plays an important role in the development and progression of chronic liver diseases. This article introduces the mechanism of pyroptosis and summarizes the role of pyroptosis in the development and progression of nonalcoholic fatty liver disease, alcoholic liver disease, viral hepatitis, liver cirrhosis, and hepatocellular carcinoma, in order to provide new ideas and methods for the prevention and treatment of liver diseases in clinical practice.

9.
Journal of Clinical Hepatology ; (12): 1415-1419, 2020.
Article in Chinese | WPRIM | ID: wpr-822204

ABSTRACT

Liver failure is a syndrome of severe liver diseases commonly seen in clinical practice, and it has a high mortality rate and thus becomes one of the critical diseases in internal medicine. Massive hepatocyte death and the extent of hepatocyte death exceeding the liver’s regenerative capacity are considered the core events in the development and progression of liver failure, and direct injury and immune-mediated inflammatory injury are the two main factors in this process. An increasing number of evidence has shown that host immune response and inflammatory cascade play an important role in the pathogenesis of liver failure. This article reviews the mechanism of action of immunoregulation (congenital and adaptive) and inflammatory injury (inflammation inducers, receptor cells, and inflammatory mediators) in the process of hepatic failure, as well as the interactions between immune response and immune cells and between inflammatory response and inflammatory factors, in order to help understand the pathogenesis of liver failure and provide new ideas for the diagnosis and treatment of liver failure and drug research and development.

10.
Article in Chinese | WPRIM | ID: wpr-813030

ABSTRACT

The advent of catheter ablation technology has changed the treatment strategy for atrial fibrillation, and the efficacy of catheter ablation is accurate with small surgical trauma. Catheter ablation treatment of atrial fibrillation is significantly better than pharmacologic therapy of anti-arrhythmia and rate control. However, the clinic data of catheter ablation of atrial fibrillation show that the recurrence rate is high. The risk factors for recurrence after catheter ablation include age, sex, body mass index, related primary disease, left atrial volume, pulmonary vein volume, gene, atrial fibrillation types, surgery and so on. Regulation of the above factors is crucial in improving the clinical efficacy and prognosis of catheter ablation of atrial fibrillation.


Subject(s)
Anti-Arrhythmia Agents , Atrial Fibrillation , Catheter Ablation , Humans , Pulmonary Veins , Recurrence , Risk Factors , Treatment Outcome
11.
Chinese Journal of Geriatrics ; (12): 528-531, 2018.
Article in Chinese | WPRIM | ID: wpr-709299

ABSTRACT

Objective To investigate the correlation between the expression levels of miRNAs and the pathogenesis of diabetes mellitus.Methods A total of 120 patients with type 2 diabetes recruited from October 2014 to October 2016 and 40 healthy individuals as a control group were included in this study,and diabetic patients were further divided into newly diagnosed group(n=40),prediabetes group(n =40),and susceptibility group (n =40).The fasting blood of four groups were collected,and real-time RT-PCR was applied to measure the expression levels of miRNAs including miR-9,miR-124a,miR-375,miR-29a,miR-126,miR-197,miR-233,and miR-320.The relationship between the pathogenesis of disease and miRNAs expression levels was analyzed.Results The levels of miRNA in the three diabetes groups were obviously higher than in the control group(P< 0.05).The miRNA expression level showed a certain trend in diabetic patients.The higher levels of miR-9,miR-124a,miR-375,and miR-29a were observed in the three diabetes groups,while the levels of miR-126,miR-197,miR-233,and miR-320 were decreased in these three groups.The expression levels of miR-9,miR 124a,miR-375,and miR-29a of miRNAs were correlated with the onset of diabetes(RR =5.96,6.32,5.24.5.69,all P< 0.05).Conclusions The level of miRNA is associated with the onset of diabetes that suggests it may be used to predict the occurrence and development of diabetes.

12.
Article in Chinese | WPRIM | ID: wpr-698754

ABSTRACT

BACKGROUND: The recombinant adenovirus has certain toxic reactions to cell growth and survival, but the determination of the optimal multiplicity of infection (MOI) is not well documented. OBJECTIVE: To study the effectiveness and toxicity of transfection of bone marrow mesenchymal stem cells (BMSCs) by adenovirus vectors with different MOIs, and to explore the effect on osteogenic capability of the cells. METHODS: BMSCs at passage 5 were infected with enhanced green fluorescent protein (EGFP) via adenovirus vectors with different MOI (0, 50, 100, 150, 200 and 250). Cell morphology and mortality rate were observed and calculated using trypan blue staining method at 24 hours after cell transfection. The transfection efficiency and the relative mRNA expression of EGFP and Runx2 were analyzed respectively by inverted florescent microscopy and real-time quantitative PCR at 48 hours after cell transfection. The activity of BMSCs transfected with different MOIs was evaluated by MTT, and the optimal MOI was determined thereafter. RESULTS AND CONCLUSION: With the increase of MOI, BMSCs showed decreased adherent ability, and even some cells aged and died. The mortality rate of BMSCs transfected for 24 hours at a MOI of 0 to 250 was 5.80%, 6.67%, 7.95%, 7.76%, 10.35% and 11.18%, respectively, indicating that the mortality rate of BMSCs is positively correlated with the MOI. The transfection efficiency changed insignificantly when the MOI was greater than 100. On the contrary, the cellular viability and osteogenic differentiation capability of rabbit BMSCs were receded when the MOI level was up to 200-250. The study discovered that the suitable scope of MOI to transfect BMSCs is 50-150, and the optimal MOI is 100.

13.
Article in Chinese | WPRIM | ID: wpr-698110

ABSTRACT

Objective To investigate the neural activity in the central auditory pathway by using a tinnitus an-imal model .Methods Twenty -four rats were randomly divided into the control ,acute salicylate treatment ,chronic salicylate treatment ,and recovery groups .The gap prepulse inhibition of acoustic startle test was used to confirm tinnitus -like behavior .After delivery of an intravenous bolus of fluorine -18 fluorodeoxyglucose (18F -FDG ) , small animal positron emission tomography scans were performed on rats .Results Only rats in chronic salicylate -treatment group showed evidence of experiencing tinnitus .The SUV ratios of the AC were significantly greater in the acute salicylate treatment group than in the control group (P<0 .01) ,suggesting relatively increased metabolism in the two brain regions of the rats in this group .The SUV ratios of the IC and AC (P<0 .01) ,but not of the CRB (P>0 .05) were greater in the chronic salicylate treatment group than in the control groups .There was a significant difference in whole brain SUVs between the control and acute salicylate treatment groups (P<0 .01) ,the whole brain SUVs in chronic salicylate treatment group were a little higher but showed no significant difference (P>0 .05) .There was no significant difference in the SUVs between the control and recovery groups (P>0 .05) .Conclu-sion These findings indicate that long -term salicylate administration induced tinnitus in rats and may have en-hanced neural activity corresponded to the up -regulated metabolic rate in our study .Alterations to neuroplasticity of the CNS may lead to tinnitus .

14.
Article in English | WPRIM | ID: wpr-773561

ABSTRACT

A series of new hybrids of dehydroandrographolide (TAD), a biologically active natural product, bearing nitric oxide (NO)-releasing moieties were synthesized and designated as NO-donor dehydroandrographolide. The biological activities of target compounds were studied in human erythroleukemia K562 cells and breast cancer MCF-7 cells. Biological evaluation indicated that the most active compound I-5 produced high levels of NO and inhibited the proliferation of K562 (IC 1.55 μmol·L) and MCF-7 (IC 2.91 μmol·L) cells, which were more potent than the lead compound TAD and attenuated by an NO scavenger. In conclusion, I-5 is a novel hybrid with potent antitumor activity and may become a promising candidate for future intensive study.


Subject(s)
Antineoplastic Agents , Chemistry , Cell Proliferation , Diterpenes , Chemistry , Pharmacology , Drug Design , Drug Screening Assays, Antitumor , Humans , K562 Cells , MCF-7 Cells , Nitric Oxide , Chemistry , Pharmacology , Structure-Activity Relationship
15.
Article in English | WPRIM | ID: wpr-776917

ABSTRACT

Cisplatin and other platinum-based drugs are used frequently for treatment of lung cancer. However, their clinical performance are usually limited by drug resistance or toxic effects. Carnosic acid, a polyphenolic diterpene isolated from Rosemary (Rosemarinus officinalis), has been reported to have several pharmacological and biological activities. In the present study, the combination effect of cisplatin plus carnosic acid on mouse LLC (Lewis lung cancer) xenografts and possible underlying mechanism of action were examined. LLC-bearing mice were treated with intraperitoneal injection with cisplatin, oral gavage with carnosic acid, or combination with cisplatin and carnosic acid, respectively. Combination of carnosic acid and cisplatin yielded significantly better anti-growth and pro-apoptotic effects on LLC xenografts than drugs alone. Mechanistic study showed that carnosic acid treatment boosted the function of CD8 T cells as evidenced by higher IFN-γ secretion and higher expression of FasL, perforin as well as granzyme B. In the meantime, the proportion of MDSC (myeloid-derived suppressor cells) in tumor tissues were reduced by carnosic acid treatment and the mRNA levels of iNOS2, Arg-1, and MMP9, which are the functional markers for MDSC, were reduced. In conclusion, our study proved that the functional suppression of MDSC by carnosic acid promoted the lethality of CD8 T cells, which contributed to the enhancement of anti-lung cancer effect of cisplatin.


Subject(s)
Animals , Antineoplastic Agents , CD8-Positive T-Lymphocytes , Allergy and Immunology , Carcinoma, Lewis Lung , Drug Therapy , Genetics , Allergy and Immunology , Cell Line, Tumor , Cisplatin , Abietanes , Drug Synergism , Humans , Interferon-gamma , Genetics , Allergy and Immunology , Lung Neoplasms , Drug Therapy , Genetics , Allergy and Immunology , Matrix Metalloproteinase 9 , Genetics , Mice , Mice, Inbred C57BL , Myeloid-Derived Suppressor Cells , Allergy and Immunology , Plant Extracts , Rosmarinus , Chemistry
16.
Article in English | WPRIM | ID: wpr-812350

ABSTRACT

A series of new hybrids of dehydroandrographolide (TAD), a biologically active natural product, bearing nitric oxide (NO)-releasing moieties were synthesized and designated as NO-donor dehydroandrographolide. The biological activities of target compounds were studied in human erythroleukemia K562 cells and breast cancer MCF-7 cells. Biological evaluation indicated that the most active compound I-5 produced high levels of NO and inhibited the proliferation of K562 (IC 1.55 μmol·L) and MCF-7 (IC 2.91 μmol·L) cells, which were more potent than the lead compound TAD and attenuated by an NO scavenger. In conclusion, I-5 is a novel hybrid with potent antitumor activity and may become a promising candidate for future intensive study.


Subject(s)
Antineoplastic Agents , Chemistry , Cell Proliferation , Diterpenes , Chemistry , Pharmacology , Drug Design , Drug Screening Assays, Antitumor , Humans , K562 Cells , MCF-7 Cells , Nitric Oxide , Chemistry , Pharmacology , Structure-Activity Relationship
17.
Article in English | WPRIM | ID: wpr-812339

ABSTRACT

Cisplatin and other platinum-based drugs are used frequently for treatment of lung cancer. However, their clinical performance are usually limited by drug resistance or toxic effects. Carnosic acid, a polyphenolic diterpene isolated from Rosemary (Rosemarinus officinalis), has been reported to have several pharmacological and biological activities. In the present study, the combination effect of cisplatin plus carnosic acid on mouse LLC (Lewis lung cancer) xenografts and possible underlying mechanism of action were examined. LLC-bearing mice were treated with intraperitoneal injection with cisplatin, oral gavage with carnosic acid, or combination with cisplatin and carnosic acid, respectively. Combination of carnosic acid and cisplatin yielded significantly better anti-growth and pro-apoptotic effects on LLC xenografts than drugs alone. Mechanistic study showed that carnosic acid treatment boosted the function of CD8 T cells as evidenced by higher IFN-γ secretion and higher expression of FasL, perforin as well as granzyme B. In the meantime, the proportion of MDSC (myeloid-derived suppressor cells) in tumor tissues were reduced by carnosic acid treatment and the mRNA levels of iNOS2, Arg-1, and MMP9, which are the functional markers for MDSC, were reduced. In conclusion, our study proved that the functional suppression of MDSC by carnosic acid promoted the lethality of CD8 T cells, which contributed to the enhancement of anti-lung cancer effect of cisplatin.


Subject(s)
Abietanes , Animals , Antineoplastic Agents , CD8-Positive T-Lymphocytes , Allergy and Immunology , Carcinoma, Lewis Lung , Drug Therapy , Genetics , Allergy and Immunology , Cell Line, Tumor , Cisplatin , Drug Synergism , Humans , Interferon-gamma , Genetics , Allergy and Immunology , Lung Neoplasms , Drug Therapy , Genetics , Allergy and Immunology , Matrix Metalloproteinase 9 , Genetics , Mice , Mice, Inbred C57BL , Myeloid-Derived Suppressor Cells , Allergy and Immunology , Plant Extracts , Rosmarinus , Chemistry
18.
Article in Chinese | WPRIM | ID: wpr-692201

ABSTRACT

OBJECTIVE To observe the changes of synaptic ultrastructures in the rat auditory center after long-term salicylate administration and to elucidate the role of neuroplasticity in some areas of the CNS and its involvement in tinnitus. METHODS The rats were divided into 4 groups: the control group, the acute treatment group, the chronic treatment group, and the recovery group. We investigated ultrastructural alterations in the synapses of inferior colliculus (IC), auditory center (AC) and cerebellum (CRB) by transmission electron microscopy. RESULTS There were more synaptic vesicles (tIC=-4. 61, tAC=-7. 00, P<0. 01), with greater postsynaptic densities(tIC=-4. 72,P<0. 01; tAc=-3. 15, P<0. 05), longer synaptic active zone (tIC=-4. 89, tAC=-3. 48, P< 0. 01), and increased synaptic interface curvature (tIC=-2. 32, tAC=-3. 17, P<0. 05) in the chronic treatment group, as compared with the control group. There were more synaptic vesicles but no other changes in the acute salicylate-treatment group(tIC=-10. 57, tAC=-8. 34, tCRB=-9. 18,P <0. 01). CONCLUSION These findings showed that long-term salicylate administration have induced synaptic ultrastructural changes in the IC and AC because of neuroplasticity. These structural changes may result in increased speed and efficacy of chemical synaptic transmission. Alterations to neuroplasticity of the auditory center pathway may lead to tinnitus.

19.
Article in Chinese | WPRIM | ID: wpr-691118

ABSTRACT

<p><b>OBJECTIVE</b>To determine the association of sarcopenia with short-term postoperative function after hip replacement for femoral neck fractures.</p><p><b>METHODS</b>A prospective study of 181 consecutive patients with femoral neck fractures who underwent hip replacement from May 2014 to January 2017 were performed, including 58 males and 123 females aging from 53 to 92 years old. The general conditions were collected before surgery, skeletal muscle index(ASMI), handgrip strength were measured. Clinical outcomes were followed up including postoperative complications, time of on-site, Harris score (postoperative 2 weeks, 3, 6 months), hospitalization costs, and hospital stay. According to handgrip strength and ASMI, the patients were divided into the sarcopina group and the non-sarcopina group; according to the Harris score at the 6-month follow-up, the patients were divided into good prognosis group and poor prognosis group. Univariate analysis and binary logistic regression analysis were used to investigate whether sarcopenia was a risk factor for poor postoperative hip joint surgery.</p><p><b>RESULTS</b>All patients were followed up at 2 weeks, 3 and 6 months, postoperative early complication included wound infection in 16 cases, thrombus of lower extremity veins in 14 cases, no dislocation, prosthetic loosening and prosthesis related infections occurred. Sarcopenia was present in 82 of 181 patients(45%), Compared with non-sarcopenic patients, sarcopenic patients had a higher risk of postoperative complications, longer postoperative hospital stay, more hospital costs and lower harris scores. In Binary logistic analysis revealed that sarcopenia(=0.08), hemiarthroplasty(<0.001), diabetes(=0.016) and infection(=0.018) were important predictors of unsatisfactory postoperative function.</p><p><b>CONCLUSIONS</b>Sarcopenia is an important predictor of poor postoperative prognosis in patients with femoral neck fractures after hip replacement. The treatment for sarcopenia maybe an important way to protect patients with femoral neck fractures from poor prognosis after hip replacement.</p>

20.
Article in Chinese | WPRIM | ID: wpr-506985

ABSTRACT

Precision medicine is an emerging approach for disease treatment and prevention , which attempts to explore the effective means for protecting human health by synthetical consideration of individual variability in genes , environment and life style.Precision medicine has the prominent property of multidisciplinary intercrossing and fusion , the development of which claims rapid clinical application of advanced technology in the research of basic medical science.What kind of development opportunities are laboratory medicine confronted with under the novel medical mode? How can laboratory medicine and its researchers do to seize these opportunities and meet the challenges of precision medicine ? These questions are preliminary discussed in this paper.

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