Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 12 de 12
Filter
Add filters








Year range
1.
Article in English | WPRIM | ID: wpr-903805

ABSTRACT

Background@#The present investigation explored the therapeutic actions of oleuropein along with the possible signaling pathway involved in attenuating neuropathic pain in chronic constriction injury (CCI) and vincristine-induced neuropathic pain in male rats. @*Methods@#Four loose ligatures were placed around the sciatic nerve to induce CCI, and vincristine (50 μg/kg) was injected for 10 days to develop neuropathic pain.The development of cold allodynia, mechanical allodynia, and mechanical hyperalgesia was assessed using different pain-related behavioral tests. The levels of H2S, cystathionine-γ-lyase (CSE), cystathionine-β-synthase (CBS), orexin, and nuclear factor erythroid-2-related factor 2 (Nrf2) were measured in the sciatic nerve. @*Results@#Treatment with oleuropein for 14 days led to significant amelioration of behavioral manifestations of neuropathic pain in two pain models. Moreover, oleuropein restored both CCI and vincristine-induced decreases in H2S, CSE, CBS, orexin, and Nrf2 levels. Co-administration of suvorexant, an orexin receptor antagonist, significantly counteracted the pain-attenuating actions of oleuropein and Nrf2 levels without modulating H2S, CSE and CBS. @*Conclusions@#Oleuropein has therapeutic potential to attenuate the pain manifestations in CCI and vincristine-induced neuropathic pain, possibly by restoring the CSE, CBS, and H2S, which may subsequently increase the expression of orexin and Nrf2 to ameliorate behavioral manifestations of pain.

2.
Article in English | WPRIM | ID: wpr-896101

ABSTRACT

Background@#The present investigation explored the therapeutic actions of oleuropein along with the possible signaling pathway involved in attenuating neuropathic pain in chronic constriction injury (CCI) and vincristine-induced neuropathic pain in male rats. @*Methods@#Four loose ligatures were placed around the sciatic nerve to induce CCI, and vincristine (50 μg/kg) was injected for 10 days to develop neuropathic pain.The development of cold allodynia, mechanical allodynia, and mechanical hyperalgesia was assessed using different pain-related behavioral tests. The levels of H2S, cystathionine-γ-lyase (CSE), cystathionine-β-synthase (CBS), orexin, and nuclear factor erythroid-2-related factor 2 (Nrf2) were measured in the sciatic nerve. @*Results@#Treatment with oleuropein for 14 days led to significant amelioration of behavioral manifestations of neuropathic pain in two pain models. Moreover, oleuropein restored both CCI and vincristine-induced decreases in H2S, CSE, CBS, orexin, and Nrf2 levels. Co-administration of suvorexant, an orexin receptor antagonist, significantly counteracted the pain-attenuating actions of oleuropein and Nrf2 levels without modulating H2S, CSE and CBS. @*Conclusions@#Oleuropein has therapeutic potential to attenuate the pain manifestations in CCI and vincristine-induced neuropathic pain, possibly by restoring the CSE, CBS, and H2S, which may subsequently increase the expression of orexin and Nrf2 to ameliorate behavioral manifestations of pain.

3.
Article in Chinese | WPRIM | ID: wpr-863181

ABSTRACT

Cerebral microbleeds (CMBs) is a imaging manifestation of cerebral small vessel disease. At present, more and more opinions believe that vascular endothelial injury plays an important role in the pathogenesis of CMBs. The destruction of the blood-brain barrier and inflammatory response caused by vascular endothelial dysfunction may promote the occurrence and development of CMBs. At the same time, the deposition of hemosiderin around the lesion of CMBs may also trigger an inflammatory response. However, the relevant mechanisms and causality have not yet been fully elucidated. This article reviews the vascular endothelial inflammatory factors related to CMBs and their mechanism in the pathogenesis of CMBs.

4.
Article in Chinese | WPRIM | ID: wpr-697573

ABSTRACT

Objective To evaluate the clinical efficacy and safety of Chaigan Jieyou decoction in treating Parkinson's disease depression (PDD).Methods Applying the method of simple random contrast,this research collects 70 patients who meet the study criteria and divide the patients into two groups of 35 peoples each:Chaigan Jieyou decoction group,which received treatment of Chaigan Jieyou decoction and fluoxetine group with treatment of fluoxetine.Treatment period was 2 months.Apply the HAMD-17 (Hamilton Depression Scale),Beck Depression Scale (Beck Depression Inventory,BDI),Parkinson's composite score (unified Parkinson disease rating scale,UPDRS) and TCM symptoms rating scale to evaluate the clinical efficacy before and after treatment.Resuits Compared the two groups by HAMD Score reduction rate,there is no statistical significance in the general efficacy rate (P > 0.05);compared by the curative effect of TCM syndrome,Chaigan Jieyou decoction group is better than fluoxetine group (P < 0.05).There is statistical significance in the differences of all the HAMD,BDI,UPDRS Ⅱ,UPDRS Ⅲ scores between the two groups after the treatment (P < 0.01,P < 0.05).Compared the treatment efficacy by the HAMD scores,Chaigan Jieyou decoction can alleviate the clinical symptoms of patients of varying degrees.Conclusions Chaigan Jieyou decoction can significantly relieve depression of PDD patients,and there is exact curative effect to the mild and moderate PDD patients,improving their quality of life to a certain extent.

5.
Article in Chinese | WPRIM | ID: wpr-619223

ABSTRACT

Objective:To observe the clinical efficacy of CAD/CAM all-ceramic onlay in the restoration of molars with extensive defect after root canal therapy(RCT).Methods:260 molars with extensive defect were restored by all-ceramic onlay after RCT.The patients were followed up for 18 months.The restoration effects were assessed using USPHS criteria.Results:The success rate of CAD/CAM all-ceramic onlay was 98%.Restoration exfoliation was found in 3 cases,fractured in 2 cases.Conclusion:CAD/CAM all-ceramic onlay can be applied in restoring extensive postenor tooth-defection after RCT.

6.
Chinese Journal of General Surgery ; (12): 1008-1010, 2016.
Article in Chinese | WPRIM | ID: wpr-506182

ABSTRACT

Objective To investigate factors impacting on local recurrence in cases of primary retroperitoneal liposarcoma (RPLS) after complete enbloc resection.Methods The clinical data of 47 cases of primary retroperitoneal liposarcoma were retrospectively analyzed.Results 3-year local recurrence rate was 62%.Single and multiple regression analysis showed preoperative needle biopsy,histological subtype,tumor size and capsular invasion were all risk factors related to postoperative local recurrence in primary retroperitoneal liposarcoma undergoing complete resection (P < 0.05).Conclusion Preoperative aspiration biopsy,tumor poor differentiation,large tumor size tumor extracapsular spread all lead to high local recurrence rate after complete resection of primary retroperitoneal liposarcoma.

7.
Chinese Journal of Anesthesiology ; (12): 1435-1438, 2015.
Article in Chinese | WPRIM | ID: wpr-672307

ABSTRACT

Objective To investigate the role of mitochondrial apoptotic pathway in intrathecal doxepin?induced apoptosis in rat neurons. Methods Twenty?four adult male Wistar rats, weighing 250-300 g, in which intrathecal catheters were successfully implanted without complications, were randomly divided into 4 groups ( n=6 each) using a random number table: control group ( group C) and different concentrations of doxepin groups (D5, D10 and D20 groups). In D5, D10 and D20 groups, 5, 10, and 20 mmol∕L doxepin 0. 2 μl∕g were injected intrathecally, respectively. In group C, the equal volume of normal saline was given instead. At 6 h after intrathecal administration, the animals were sacrificed, and the lumbar segments of the spinal cords were obtained for detection of the cell apoptosis ( by TUNEL assay) , expression of Bax and Bcl?2 ( by immunohistochemistry) , release of cytochrome c ( Cyt c) , and expression of caspase?3, caspase?8 and caspase?9 mRNA ( using real?time reverse transcriptase polymerase chain reaction) . Apoptosis rate and Bcl?2∕Bax ratio were calculated. Results Compared with group C, the expression of Bax was significantly up?regulated, the expression of Bcl?2 was down?regulated, Bcl?2∕Bax ratio was decreased, the release of Cyt c and apoptosis rate were increased, and the expression of caspase?3 and caspase?9 mRNA was up?regulated in D10 and D20 groups, the expression of Bcl?2 was down?regulated in group D20 ( P0.05). The expression of Bax was significantly up?regulated, Bcl?2∕Bax ratio was decreased, the release of Cyt c and apoptosis rate were increased, and the expression of caspase?3 and caspase?9 mRNA was up?regulated in D10 and D20 groups, and the expression of Bcl?2 was down?regulated in group D20 as compared with group D5 ( P0.05) . Conclusion Mitochondrial apoptotic pathways, but not death receptor pathway, is involved in intrathecal doxepin?induced apoptosis in rat neurons.

8.
Article in Chinese | WPRIM | ID: wpr-602459

ABSTRACT

Objective Comparing with traditional teaching methods of oral histopathology, to probe whether the interactive teaching system of the digital microscope can improve the teaching effi-ciency of experiment of oral histopathology courses. Methods The subjects were the 2010 grade students (40 students) and the 2011 grade students (38 students) majoring in stomatology of South Medical University, and they were evenly divided into 2 groups at random. In the first part of the ex-periment of Grade 2010, the teaching of traditional optical microscope was used in a group of experi-mental courses, and interactive teaching with digital microscope was used in the other group. In the second part of the experiment the two groups swapped the teaching method. While in the whole exper-iment of Grade 2011, traditional optical microscopes were adopted in one group, and in contrast, the interactive teaching system of the digital microscope was adopted in the other group. The P values of the 5 indexes were less than 0.05. After the courses, the 2010 grade students received the questionnaire survey and the 2011 grade students received the quiz about the experimental course, and then the evaluation results of the two groups were compared for t inspection, using SPSS software t test. Result The questionnaire results of Grade 2010 showed that the scores of digital microscope interactive group were higher than those of traditional teaching group in satisfaction with teaching , teacher-student interaction, classroom atmosphere active degree, learning interest, and the understanding of the teach-ing content. The experiment course content test results of Grade 2011 showed that the digital interactive group students averaged 46.26, while the average score of the traditional teaching group was 42.78%. The difference between the two groups had statistical significance, P=0.001. Conclusion Compared with the traditional teaching methods, the interactive teaching system of the digital microscope can enhance the academic grades, improve the teaching efficiency of experiment of oral histopathology courses, and elevate the teaching quality.

9.
Article in Chinese | WPRIM | ID: wpr-472980

ABSTRACT

Objective To investigate the craniofacial morphologies of Sagliker syndrome.Methods Sixteen patients were selected in this study that was diagnosed by the Department of Nephrology and the Center of Dentistry of China-Japan Friendship Hospital.They were divided into 2 groups:Sagliker syndrome group(SS) including 8 patients,and secondary hyperparathyroidism group (SHPT) including 8 patients.Cephalometric radiographs were taken from all the 16 patients.Results The hard issue measurement results had shown that in ∠SNA,∠ANB,∠NA/PA,∠MP/SN,∠MP/ FH,∠SArGo,and ∠ArGoGn,there were significantly statistical differences between the two groups.The measurements of SS were greater than that of SHPT.The soft issue measurement results had shown that in ∠LL-Bs-Pos,∠AsUL-FH,UL-EP,and ∠AsUL-BsUL,there were also significant differences between the two groups.The measurements of SS were greater than that of SHPT.In ∠BsLL-FH,there were evidently statistical differences between the two groups:the measurements of SS were greater than that of SHPT.Conclusions The facial hard and soft tissue of the patients with Sagliker syndrome have the special changes in morphologies.

10.
The Journal of Practical Medicine ; (24): 3732-3735, 2014.
Article in Chinese | WPRIM | ID: wpr-461668

ABSTRACT

Objective To build rat models and observe changes of behavior and monoamine of PDD. Methods The rats were injected with 6-OHDA and accepted CUMS for the establishment of PDD model , so as to assess its behavior changes and detect brain tissue monoamine contents by ELISA. Results Significant behavioral and monoamine abnormalities can be observed in model rats. Behavioral and monoamine improvement can be observed after treated with Chaigan Jieyou. Conclusion The PDD model can be established by 6-OHDA and CUMS. Chaigan Jieyou decoction may reach therapeutic purposes by increasing monoamine contents.

11.
Article in Chinese | WPRIM | ID: wpr-454846

ABSTRACT

Objective-To-investigate-the-feasibility-of-total-laparoscopic-hysterectomy-for-complicated-hysteromyoma-,-such-as-broad-ligament-myoma,-lower-uterine-fibroids,-or-cervical-myoma.-Methods-From-December-2009-to-December-2012,-we-performed-laparoscopic-hysterectomy-on-166-patients-with-complicated-hysteromyoma-and-on-170-patients-with-common-hysteromyoma-.-The-operation-time-,-intraoperative-blood-loss-,-recovery-time-of-gastrointestinal-function-,-postoperative-morbidity-,-and-the-rate-of-conversion-to-laparotomy-were-compared-between-the-two-groups-respectively-.-Results-No-significant-differences-were-detected-in-operation-time-[(112.2-±15.3)-min-vs.(110.3-±16.1)-min,-t=1.088,-P=0.277],-intraoperative-blood-loss-[(117.0-±35.3)-ml-vs.(110.8-±37.8)-ml,-t=-1.525,-P=0.128],-recovery-time-of-gastrointestinal-function-[(20.8-±2.7)-h-vs.(21.3-±2.3)-h,-t=1.797,-P=0.073],-postoperative-morbidity-[6.3%(10/159)-vs.7.3%(12/165),χ2-=0.124,-P=0.725],-and-the-rate-of-conversion-to-laparotomy-(4.2%vs.2.9%,χ2-=0.397,-P=0.529)-between-the-two-groups-.Both-the-groups-were-followed-up-at-1,-2,-and-6-months-after-procedure-,-and-none-of-the-patients-showed-severe-postoperative-complications-.-Conclusions-Total-laparoscopic-hysterectomy-for-complicated-hysteromyoma-is-feasible-.To-prevent-complications-,-it-should-be-converted-to-laparotomy-timely-when-the-myoma-has-a-wide-base-or-is-located-at-lower-position-.

12.
Article in Chinese | WPRIM | ID: wpr-405597

ABSTRACT

To investigate the feasibility of construction of engineered skin substitutes with adipose tissue derived stem cells ( ADSCs)from GFP-mouse and fibrin glue. Methods: Adipose tissues were isolated from GFP-C57BL mouse groin, the fat was cut into pieces and incubated in a collagenase solution to establish ADSCs culture. The differentiation ability into adipocytes and osteoblasts of the ADSCs were investigated. The amplified cells in vitro were seeded in fibrin glue to form tissue engineered skin substitutes. Fifteen C57BL mice were randomly divided into three groups; experimental group was treated with ADSCs-fibrin glue tissue engineered active skin substitutes. Single fibrin glue and blank were served as two control groups. The materials were grafted onto total skin defects on the back of mice. The effect of wound repair was observed histologically. Results; The cultured ADSCs grew well in vitro. The cells showed characteristics of multipotent adult stem cells. ADSCs-fibrin glue shortened the wound healing time. Histological observation showed that thicker epithelium was formed and collagen fibers arranged in order in the ADSCs-fibrin glue experimental group,the number of fibroblasts was significantly increased as compared with those in single fibrin glue group or in the blank control group. Conclusion : GFP- ADSCs combined with fibrin glue can accelerate the mouse skin wound healing. It suggests that ADSCs with fibrin glue maybe an optional method of engineered skin reconstruction for wound repair.

SELECTION OF CITATIONS
SEARCH DETAIL