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1.
Frontiers of Medicine ; (4): 284-292, 2020.
Article in English | WPRIM | ID: wpr-827865

ABSTRACT

The features of myocardial strains from speckle-tracking echocardiography (STE) have not been well defined in fulminant myocarditis (FM) patients. In this study, changes in the left ventricular ejection fraction (LVEF) and global and layer-specific myocardial strains over time were monitored. We aimed to determine the echocardiographic patterns of FM and ascertain their significance in FM treatment. Twenty patients who were clinically diagnosed with FM and received mechanical life support were prospectively enrolled. Conventional echocardiographic measurements were obtained, and serial strain echocardiography was performed from admission to hospital discharge until LVEF recovery (> 50%). Global/regional peak systolic longitudinal strains (GLS/RLS) and layer-specific longitudinal strains were quantified, and their changes with time were monitored in 14 FM patients. All patients had severely impaired cardiac function. Steep improvement in LVEF and GLS were observed within 6 days. Layer-specific strain analysis showed that reduction at admission or recovery at discharge in the endocardium and epicardium strains were equal. In conclusion, FM patients who received mechanical circulatory supports exhibited steep improvement in ventricular function within 6 days. The patchy and diffused distribution pattern of reduced RLS and equally and severely impaired strain in the endocardium and epicardium are valuable features in the diagnosis of FM.

2.
Article in Chinese | WPRIM | ID: wpr-815974

ABSTRACT

In the 2017 AHA Hypertension Guidelines, BP≥130/80 mm Hg was used as the new diagnostic criterion for hypertension, and risk stratification was emphasized according to the 10-year CVD risk. Lifestyle intervention, without pharmacological treatment, was recommended for low-risk patients whose BP≥130/80 mmHg. However, high risk patients should receive antihypertensive pharmacological treatment as soon as their BP≥130/80 mmHg. In the 2018 European Guidelines for the management of hypertension, the definition and classification of office hypertension still followed the standard of the 2013's edition, but more aggressive therapeutic strategies were recommended.Hypertension can be divided into primary hypertension and secondary hypertension. To screen and diagnose secondary hypertension, it is necessary not only to construct a systematic and standardized method to avoid missed diagnosis and misdiagnosis but also to avoid screening blindly.

3.
Article in Chinese | WPRIM | ID: wpr-735750

ABSTRACT

Global longitudinal strain (GLS) at rest on two-dimensional speckle tracking echocardiography (2D STE) was demonstrated to help detect coronary artery disease (CAD).However,the optimal cut-off point of GLS and its diagnostic power for detecting critical CAD in non-diabetes mellitus (DM) patients are unknown.In the present study,211 patients with suspected CAD were prospectively included,with DM patients excluded.All patients underwent echocardiography and subsequently coronary angiography within 3 days.Left ventricular (LV) GLSs were quantified by 2D STE.Territorial peak systolic longitudinal strains (TLSs) were calculated based on the perfusion territories of the 3-epicardial coronary arteries in a 17-segment LV model.Critical CAD was defined as an area stenosis ≥70% in ≥1 epicardial coronary artery (≥50% in left main coronary artery).Totally 145 patients were diagnosed as having critical CAD by coronary angiography.Significant differences were observed in all strain parameters between patients with and without critical CAD.The area under the receiver operating charcteristic (ROC) curve (AUC) for GLS in the detection of left main (LM) or threevessel CAD was 0.875 at a cut-off value of-19.05% with sensitivity of 78.1% and specificity of 72.7%,which increased to 0.926 after exclusion of apical segments (cut-off value-18.66%;sensitivity 84.4% and specificity 81.8%).The values of TLSs were significantly lower in regions supplied by stenotic arteries than in those by non-stenotic arteries.The AUC for the TLSs to identify critical stenosis of left circumflex (LCX) artery,left anterior descending (LAD) artery and right coronary artery (RCA),in order of diagnostic accuracy,was 0.818 for LCX,0.764 for LAD and 0.723 for RCA,respectively.In conclusion,in non-DM patients with suspected CAD,GLS assessed by 2D STE is an excellent predictor for LM or three-vessel CAD with high diagnostic accuracy,and a higher cut-off point than reported before should be used.Excluding apical segments in the calculation of GLS can further improve the predictive accuracy of GLS.It is unsatisfactory for TLSs to be used to identify stenotic coronary arteries.

4.
Frontiers of Medicine ; (4): 319-323, 2018.
Article in English | WPRIM | ID: wpr-772755

ABSTRACT

Antithrombin and protein C are two crucial members in the anticoagulant system and play important roles in hemostasis. Mutations in SERPINC1 and PROC lead to deficiency or dysfunction of the two proteins, which could result in venous thromboembolism (VTE). Here, we report a Chinese 22-year-old young man who developed recurrent and serious VTE in cerebral veins, visceral veins, and deep veins of the lower extremity. Laboratory tests and direct sequencing of PROC and SERPINC1 were conducted for the patient and his family members. Coagulation tests revealed that the patient presented type I antithrombin deficiency combined with decreased protein C activity resulting from a small insertion mutation c.848_849insGATGT in SERPINC1 and a short deletion variant c.572_574delAGA in PROC. This combination of the two mutations was absent in 400 healthy subjects each from southern and northern China. Then, we summarized all the mutations of the SERPINC1 and PROC gene reported in the Chinese Han population. This study demonstrates that the combination of antithrombin deficiency and decreased protein C activity can result in severe VTE and that the coexistence of different genetic factors may increase the risk of VTE.


Subject(s)
Antithrombin III , Genetics , Antithrombin III Deficiency , Genetics , China , Female , Humans , Male , Middle Aged , Mutation , Pedigree , Protein C , Genetics , Metabolism , Venous Thromboembolism , Genetics , Young Adult
5.
Acta Physiologica Sinica ; (6): 591-599, 2018.
Article in English | WPRIM | ID: wpr-777226

ABSTRACT

Kidney diseases are important causes of mortality world widely. Renal microvascular dysfunction plays a pivotal role in the development of kidney diseases. Pharmacological and biochemical tools have been used to conduct detailed studies on the metabolization of arachidonic acid by cytochrome P450 (CYP450) in renal microvasculature. CYP450 epoxygenase metabolites epoxyeicosatrienoic acids (EETs) are mainly produced in renal microvessels. EETs exhibit renoprotective effects through vasodilation, anti-hypertension, anti-apoptosis and anti-inflammation, and were reported as therapeutic targets of renal diseases. However, the ability of the kidney in generating EETs is reduced in renal diseases. Recently, the studies from transgenic animal overexpressing CYP450 epoxygenases and application of soluble epoxide hydrolase inhibitors revealed that increasing of EETs exhibits renoprotective effects in vivo. The present review focuses on the protective mechanisms of EETs in kidney physiology and diseases.


Subject(s)
Animals , Animals, Genetically Modified , Arachidonic Acid , Metabolism , Cytochrome P-450 Enzyme System , Physiology , Disease Models, Animal , Humans , Inflammation , Kidney , Physiology , Kidney Diseases , Vasodilation
6.
Article in Chinese | WPRIM | ID: wpr-737218

ABSTRACT

Global longitudinal strain (GLS) at rest on two-dimensional speckle tracking echocardiography (2D STE) was demonstrated to help detect coronary artery disease (CAD).However,the optimal cut-off point of GLS and its diagnostic power for detecting critical CAD in non-diabetes mellitus (DM) patients are unknown.In the present study,211 patients with suspected CAD were prospectively included,with DM patients excluded.All patients underwent echocardiography and subsequently coronary angiography within 3 days.Left ventricular (LV) GLSs were quantified by 2D STE.Territorial peak systolic longitudinal strains (TLSs) were calculated based on the perfusion territories of the 3-epicardial coronary arteries in a 17-segment LV model.Critical CAD was defined as an area stenosis ≥70% in ≥1 epicardial coronary artery (≥50% in left main coronary artery).Totally 145 patients were diagnosed as having critical CAD by coronary angiography.Significant differences were observed in all strain parameters between patients with and without critical CAD.The area under the receiver operating charcteristic (ROC) curve (AUC) for GLS in the detection of left main (LM) or threevessel CAD was 0.875 at a cut-off value of-19.05% with sensitivity of 78.1% and specificity of 72.7%,which increased to 0.926 after exclusion of apical segments (cut-off value-18.66%;sensitivity 84.4% and specificity 81.8%).The values of TLSs were significantly lower in regions supplied by stenotic arteries than in those by non-stenotic arteries.The AUC for the TLSs to identify critical stenosis of left circumflex (LCX) artery,left anterior descending (LAD) artery and right coronary artery (RCA),in order of diagnostic accuracy,was 0.818 for LCX,0.764 for LAD and 0.723 for RCA,respectively.In conclusion,in non-DM patients with suspected CAD,GLS assessed by 2D STE is an excellent predictor for LM or three-vessel CAD with high diagnostic accuracy,and a higher cut-off point than reported before should be used.Excluding apical segments in the calculation of GLS can further improve the predictive accuracy of GLS.It is unsatisfactory for TLSs to be used to identify stenotic coronary arteries.

7.
JCPSP-Journal of the College of Physicians and Surgeons Pakistan. 2015; 25 (9): 640-643
in English | IMEMR | ID: emr-168743

ABSTRACT

To systemically investigate the association between the polymorphism [rs3118869] in cathepsin L enzyme gene with hypertension in three ethnic groups [Han, Kazak and Uygur] in China. Case-control study. Department of Cardiology, The First Affiliated Hospital, Shihezi Medical College, Shihezi University and Department of Internal Medicine and Genetic Diagnosis Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, from January 2013 to May 2014. This case-control study included 1224 patients [422 Uygur, 425 Kazak and 377 Han individuals] with hypertension and 967 healthy unrelated individuals [339 Uygur, 337 Kazak and 291 Han individuals] as controls. The participants came from three ethnic groups [Han, Kazak and Uygur] which were recruited from Xinjiang Province of China. The polymorphism [rs3118869] of the human cathepsin L gene was genotyped using the TaqMan 5' nuclease assay. Binary logistic regression was built to determine the association of polymorphism with hypertension. The genotype distribution of polymorphism was not significantly different in three ethnic groups. The rs3118869 polymorphism was significantly associated with Essential Hypertension [EH] in co-dominant model [A/C vs. C/C] in total people [OR = 0.697, 95% CI = 0.520 -0.932, p = 0.015], the same result was obtained in recessive model [C/C + A/C vs. A/A] in total people [OR = 0.689, 95% CI = 0.522 -0.910, p = 0.009]. Similar finding of rs3118869 in recessive model [C/C + A/C vs. A/A] was also observed after adjusting the variable to the covariates age [OR = 0.629, 95% CI = 0.464 - 0853, p = 0.003]. The study results indicate the A-allele of rs3118869 is a protective factor in hypertension

8.
Chinese Journal of Cardiology ; (12): 477-481, 2012.
Article in Chinese | WPRIM | ID: wpr-275021

ABSTRACT

<p><b>OBJECTIVE</b>To establish the high-resolution melting curve (HRM) approach for genotyping CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) and explore its value on estimation of the Warfarin initial dose in comparison with various traditional genotyping methods.</p><p><b>METHODS</b>CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) genotyping was detected in 100 patients receiving Warfarin therapy by the newly developed HRM method and traditional genotyping methods including PCR-restriction fragment length polymorphism (PCR-RFLP), TaqMan probe and DNA sequencing.</p><p><b>RESULTS</b>The results of the genotypes obtained from above mentioned methods to detect CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) were similar and consistent. The HRM method is simpler, more economical, and faster compared to the traditional methods. The frequencies of the VKORC1-1639 AA, AG, GG genotypes from these 100 clinical samples were 73 (73%), 23 (23%), 4 (4%), respectively; Frequencies of the CYP2C9 1075 AA, AC, CC genotypes were 94(94%), 6 cases (6%), 0, respectively.</p><p><b>CONCLUSIONS</b>HRM approach can effectively detect CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) polymorphisms and this method is simpler, more economical, and faster compared to the traditional methods for detecting CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) polymorphisms.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Aryl Hydrocarbon Hydroxylases , Genetics , Cytochrome P-450 CYP2C9 , Dose-Response Relationship, Drug , Female , Freezing , Genotype , Genotyping Techniques , Humans , Male , Middle Aged , Mixed Function Oxygenases , Genetics , Polymorphism, Restriction Fragment Length , Vitamin K Epoxide Reductases , Warfarin , Therapeutic Uses , Young Adult
9.
Chinese Journal of Cardiology ; (12): 1101-1104, 2011.
Article in Chinese | WPRIM | ID: wpr-268250

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the association between rs751141 gene polymorphisms in EPHX2 gene and essential hypertension in Kazak and Han in Xinjiang.</p><p><b>METHODS</b>A total of 267 essential hypertensive patients in Kazaks, 368 essential hypertensive patients in Hans, 284 normotensive controls in Kazaks and 348 normotensive controls in Hans were enrolled in this study. TaqMan assay was used to detect the rs751141 G/A gene polymorphisms of EPHX2 gene.</p><p><b>RESULTS</b>The rs751141 G/A genotype frequencies for GA + AA genotypes was 40.2 percent in essential hypertensive subjects and 52.0 percent in control subjects in Hans, respectively. The genotype frequencies were significant difference between the two groups in Hans in Xinjiang (P < 0.01). The rs751141G/A gene polymorphism had no significant difference between essential hypertensive patients and normotensive controls in Kazaks in Xinjiang (P > 0.05).</p><p><b>CONCLUSION</b>The essential hypertension in Kazaks in Xinjiang is not associated with rs751141G/A gene polymorphism of EPHX2 gene, but the essential hypertension in Hans in Xinjiang is associated with rs751141G/A allele gene polymorphism of EPHX2 gene. A type of rs751141 allele gene polymorphism may be the independent protective factor of essential hypertension in Hans in Xinjiang.</p>


Subject(s)
Adult , Alleles , Asian Continental Ancestry Group , Genetics , China , Epidemiology , Epoxide Hydrolases , Genetics , Gene Frequency , Genotype , Humans , Hypertension , Epidemiology , Genetics , Middle Aged , Polymorphism, Single Nucleotide
10.
Chinese Journal of Cardiology ; (12): 798-800, 2010.
Article in Chinese | WPRIM | ID: wpr-244143

ABSTRACT

<p><b>OBJECTIVE</b>To compare plasma asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase inhibitor, and cystatin C levels in patients with or without coronary artery disease (CAD).</p><p><b>METHODS</b>We recruited 87 CAD patients (39 with acute myocardial infarction and 48 with unstable angina pectoris) and 51 non-CAD controls. Plasma ADMA was measured by HPLC, cystatin C by particle-enhanced immunonephelometric assay (N Latex cystatin C, Dade Behring) with nephelometer (BNII, Dade Behring). CAD patients were further divided into low cystatin C group (< 1.0 mg/L, 36 cases) and high cystatin C group (> 1.0 mg/L, 51 cases).</p><p><b>RESULTS</b>(1) The plasma levels of ADMA [(0.47 ± 0.15) µmol/L vs. (0.37 ± 0.15) µmol/L], SDMA [(0.39 ± 0.19) µmol/L vs. (0.28 ± 0.12) µmol/L] and cystatin C [(1.16 ± 0.32) mg/L vs. (0.73 ± 0.16) mg/L] were significantly higher in CAD patients than in controls (all P < 0.05). The plasma L-Arg was significantly lower in CAD patients than in controls [(59.4 ± 19.4) µmol/L vs. (83.7 ± 19.6) µmol/L, P < 0.05]. (2) Plasma ADMA was similar in CAD patients with low cystatin C level and controls [(0.42 ± 0.12) µmol/L vs. (0.39 ± 0.15) µmol/L, P = 0.251] and Plasma ADMA was significantly higher in CAD patients with high cystatin C level than in controls [(0.50 ± 0.17) µmol/L vs. (0.39 ± 0.15) µmol/L, P < 0.05].</p><p><b>CONCLUSION</b>ADMA levels were significantly increased only in CAD patients with elevated cystatin C levels but not in CAD patients with normal renal function. The reported relationship between coronary heart disease and ADMA may not be direct, but could be secondary due to reduced renal function.</p>


Subject(s)
Aged , Arginine , Blood , Case-Control Studies , Coronary Disease , Blood , Cystatin C , Blood , Female , Humans , Male , Middle Aged
11.
Chinese Journal of Cardiology ; (12): 147-151, 2010.
Article in Chinese | WPRIM | ID: wpr-341265

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the characteristics of a new clinical syndrome, including throat infection, neck spinal disease, chest pain and cardiac response.</p><p><b>METHODS</b>A total of 165 patients with above mentioned symptoms admitted to Tongji hospital from 2003 to 2005 were included in this study and underwent further medical history inquiry, physical examination and laboratory tests. Eighty-five healthy subjects served as controls. Serum myocardial auto-antibodies against beta(1)-adrenoceptor, alpha-myosin heavy chain, M(2)-muscarinic receptor and adenine-nucleotide translocator were detected, inflammatory cytokines, high sensitivity C-reaction protein, serum antibodies against Coxsackie virus-B, cytomegalovirus, Mycoplasma pneumoniae and Chlamydia pneumoniae were determined and lymphocyte subclasses were assayed by flow cytometry.</p><p><b>RESULTS</b>All patients had a complex of four symptoms or tetralogy: (1) persistent throat or upper respiratory tract infection; (2) neck pain; (3) chest pain; (4) chest depression or dyspnea, some of them with anxiety. Anti-myocardial auto-antibodies (AMCA) were present in all patients vs. 8% in controls. TNF-alpha, IL-1 and IL-6 were significantly higher in patients than controls (P < 0.01). CD3(+) and CD4(-)CD8(+) lymphocytes were significantly higher and CD56(+) lymphocytes lower in patients than those in controls (P < 0.01). The ratios of serum pathogen antibodies positive against Coxsackie virus-B, cytomegalovirus, Mycoplasma pneumoniae and Chlamydia pneumoniae were all significantly higher in patients than in controls.</p><p><b>CONCLUSIONS</b>These data led to identification of a persistent respiratory infection-related clinical syndrome, including persistent throat infection, neck spinal lesion, rib cartilage inflammation, symptoms of cardiac depression and dyspnea with or without anxiety.</p>


Subject(s)
Adolescent , Adult , Aged , Anxiety , Diagnosis , Case-Control Studies , Chest Pain , Diagnosis , Female , Heart Diseases , Diagnosis , Humans , Inflammation , Male , Middle Aged , Neck Pain , Diagnosis , Respiratory Tract Diseases , Diagnosis , Microbiology , Spinal Diseases , Diagnosis , Syndrome , Young Adult
12.
Chinese Journal of Cardiology ; (12): 354-356, 2010.
Article in Chinese | WPRIM | ID: wpr-341217

ABSTRACT

<p><b>OBJECTIVE</b>To observe the impact of growth-factor hormone control on the outcome of acromegalic cardiomyopathy by reviewing cases from our own center and from literatures.</p><p><b>METHODS</b>Two cases of acromegalic cardiomyopathy from Tongji hospital and 29 acromegalic cardiomyopathy cases with fully accessible data retrieved from PubMed and CNKI websites were included in present study for analysis. They were divided into "Controlled (< 5 microg/L)" or "Uncontrolled" group according to the serum level of growth factor hormone after treatments. Outcome of patients was evaluated by symptom, NYHA class, LV size and function status.</p><p><b>RESULTS</b>Incidence of patients with improved symptoms and cardiac performance was significantly higher in "Controlled" group (18/19) compared to those in "Uncontrolled" group (0/12; P < 0.01, chi(2) = 27.1). Post-treatment growth-factor hormone level < 5 microg/L is significantly associated with a satisfactory outcome of acromegalic cardiomyopathy (r = 0.935, P < 0.01).</p><p><b>CONCLUSIONS</b>Control of serum growth-factor concentration to a value < 5 microg/L is critical and associated with a favorable outcome for patients with acromegalic cardiomyopathy.</p>


Subject(s)
Acromegaly , Therapeutics , Aged, 80 and over , Cardiomyopathies , Therapeutics , Human Growth Hormone , Therapeutic Uses , Humans , Male , Middle Aged , Treatment Outcome
13.
Chinese Journal of Cardiology ; (12): 537-541, 2009.
Article in Chinese | WPRIM | ID: wpr-236459

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the efficacy of CD151 gene delivery in promoting blood perfusion in swines after myocardial infarction.</p><p><b>METHODS</b>Swines received coronary artery ligation and intramyocardial injection with rAAV-CD151, rAAV-anti-CD151 or rAAV-GFP. Eight weeks after vector injection, Western blot, immunostaining and 13N-labeled NH3 PET were performed to detect gene expression and biological effects of various treatments.</p><p><b>RESULTS</b>High level of CD151 protein expression was detected in the rAAV-CD151 group. The capillary density in the rAAV-CD151 group [(83.8 +/- 6.7) n/mm2] was significantly higher than that in the control group [(33.2 +/- 4.5) n/mm2] and rAAV-GFP group [(41.6 +/- 5.6) n/mm2] (all P<0.05); the arteriole density in the rAAV-CD151 group [(16.4 +/- 2.5) n/mm2] was also higher than that in the control group [(6.6 +/- 2.3) n/mm2] and the rAAV-GFP group [(8.4 +/- 1.6) n/mm2] (all P<0.05). However, the lowest capillary density and arteriole density were evidenced in rAAV-anti-CD151 group. Myocardial blood perfusion was significantly increased in rAAV-CD151 group and significantly reduced in rAAV-anti-CD151 group (all P<0.05 vs. control).</p><p><b>CONCLUSION</b>Intramyocardial injection of rAAV-CD151 could enhance the myocardial express of CD151 protein, increase capillary and arteriole densities and improve blood perfusion in swine with myocardial infarction.</p>


Subject(s)
Animals , Antigens, CD , Genetics , Coronary Occlusion , Therapeutics , Dependovirus , Genetics , Female , Gene Transfer Techniques , Genetic Therapy , Genetic Vectors , Humans , Male , Myocardial Infarction , Therapeutics , Neovascularization, Physiologic , Swine , Swine, Miniature , Tetraspanin 24 , Treatment Outcome
14.
Chinese Journal of Biotechnology ; (12): 1107-1111, 2007.
Article in Chinese | WPRIM | ID: wpr-276153

ABSTRACT

According to published nucleotide sequences, ORF4 gene of barley yellow dwarf virus GAV (BYDV-GAV) was synthesized by reverse transcription-polymerase chain reaction (RT-PCR). The BYDV-GAV ORF4 gene was expressed in baculovirus -insect cell expression system efficiently, and western bolt analysis confirmed its expression product. Confocal laser scanning microscopy showed that GFP: ORF4 fusion protein was associated with the nuclear envelope of insect cells. By expressing the N- and C-terminal regions of ORF4-encoding product (P4) in insect cells combined with structure prediction, it was found that the N-terminal region of P4 containing four a-helices is required for targeting P4 to the nuclear envelope. These results provide a base for biological function of ORF4 gene during systemic infection of BYDV-GAV in host plants further.


Subject(s)
Amino Acid Sequence , Animals , Baculoviridae , Genetics , Metabolism , Genes, Plant , Genetics , Green Fluorescent Proteins , Genetics , Insecta , Genetics , Metabolism , Luteovirus , Genetics , Molecular Sequence Data , Open Reading Frames , Genetics , Recombinant Fusion Proteins , Genetics , Metabolism
15.
Chinese Journal of Cardiology ; (12): 873-876, 2006.
Article in Chinese | WPRIM | ID: wpr-238500

ABSTRACT

<p><b>OBJECTIVE</b>To screen primary aldosteronism cases with ARR (aldosterone/plasma renin activity, ARR) from patients with hypertension, and to evaluate the diagnosis value of ARR in primary aldosteronism cases and analysis the clinical characters of primary aldosteronism cases.</p><p><b>METHODS</b>Nine hundred and two patients with hypertension were collected, the plasma aldosterone concentration to plasma renin activity ratio were detected by radio-immunity method, after that, ARR were calculated. Retrospective analysis was made of clinical data in 126 primary aldosteronism cases, which ARR were over 25.</p><p><b>RESULTS</b>One hundred and twenty-six cases (14%) were diagnosed as primary aldosteronism, and of them, 49 cases had hypokalemia. 25 patients received surgical operation and the rate of efficiency and cure of surgery treatment were 100% and 48%, respectively. The rate of efficiency and cure of drug treatment was 89% and 24% respectively.</p><p><b>CONCLUSIONS</b>Primary aldosteronism affects over 10% of patients with hypertension in China. Patients with hypertension and most patients with treatment-resistant hypertension should undergo screening for primary aldosteronism with ARR. A high ARR is a positive screening test result, a finding that warrants confirmatory testing.</p>


Subject(s)
Aldosterone , Blood , Clinical Chemistry Tests , Follow-Up Studies , Humans , Hyperaldosteronism , Diagnosis , Hypertension , Blood , Male , Middle Aged , Potassium , Blood , Renin , Blood , Renin-Angiotensin System
16.
Chinese Journal of Cardiology ; (12): 54-59, 2006.
Article in Chinese | WPRIM | ID: wpr-252983

ABSTRACT

<p><b>OBJECTIVE</b>To selectively knockdown the expression of Angiotensin II receptor subtype 1a (AT1aR) in rat vascular smooth muscle cells (VSMCs) by RNA interference and the sequential effects on cellular viability and proliferation.</p><p><b>METHODS</b>The primary cultured rat aortic VSMCs were transfected by plasmids pAT1a-shRNA1 and pAT1a-shRNA2, each carrying an U6 promoter and an AT1a-specific shRNA-coding template sequence, or by a control plasmid pGenesil-Control (pCon) carrying a nonspecific shRNA-coding sequence. The mRNA and protein expressions of AT1a, AT2 were analyzed by semi-quantified RT-PCR and Western blot, respectively and normalized to the internal control gene beta-actin. Cellular viability and proliferation were determined with methylthiazoletetrazolium (MTT) assay.</p><p><b>RESULTS</b>AT1a mRNA and protein were reduced by 82% and 69% by pAT1a-shRNA1, 77% and 56% by pAT1a-shRNA2, respectively while no change was found in pCon treated VSMCs. AT2 receptor level in VSMCs remains unchanged after various treatments. The A(490nm) values obtained by MTT measurements were similar among groups in the absence of Ang II but decreased significantly in pAT1a-shRNA1 and pAT1a-shRNA2 treated VSMCs in the presence of Ang II.</p><p><b>CONCLUSION</b>RNA interference can selectively knockdown AT1a expression in cultured VSMCs and attenuate the Ang II induced cell proliferation. Future studies are warranted to explore the potential role of RNA interference on AT1 function and as a new gene therapy tool for cardiovascular diseases.</p>


Subject(s)
Animals , Cells, Cultured , Gene Knockdown Techniques , Male , Muscle, Smooth, Vascular , Metabolism , Plasmids , RNA Interference , RNA, Small Interfering , Rats , Rats, Sprague-Dawley , Receptors, Angiotensin , Metabolism , Transfection
17.
Chinese Journal of Cardiology ; (12): 530-536, 2006.
Article in Chinese | WPRIM | ID: wpr-295281

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects and related mechanisms of bezafibrate, a ligand of peroxisome proliferator-activated receptors alpha (PPARalpha), on endothelial nitric oxide synthase (eNOS).</p><p><b>METHODS</b>Firstly, in cultured bovine aorta endothelial cells (BAEC), the effects of bezafibrate on eNOS mRNA and protein levels were investigated by Northern blot and Western blot. The half-life of eNOS mRNA and NO production determined from BAECs treated with bezafibrate were performed by real-time quantitative PCR and NO assay. Next, the effects of bezafibrate on signal pathways in BAEC, through inhibitors of PPARalpha, PI3 kinase and MAPK, were investigated by Western blot. Then luciferase reporter gene driven by human eNOS promoter were cloned and transfected endothelial cells to see the effects of bezafibrate on eNOS promoter-driven luciferase activity.</p><p><b>RESULTS</b>In cultured BAEC, bezafibrate significantly upregulated eNOS expressions at protein and mRNA levels in a concentration-dependent fashion (50 - 200 micromol/L) (P < 0.05), and increased nitric oxide (NO) production, respectively[control (14.97 +/- 1.29) micromol/L, different concentration groups (25.12 +/- 1.25) micromol/L, (30.12 +/- 1.85) micromol/L, (33.47 +/- 1.22) micromol/L], and enhanced phosphorylation of eNOS-ser-1179 site (P < 0.05), but not eNOS-thr-497 site. Through real-time quantitative PCR, bezafibrate prolonged eNOS mRNA half-life from 3.1 hour to 6.1 hour were demonstrated. Further studies showed that bezafibrate treatments significantly enhanced dose-dependently luciferase activity (relative luciferase activity in 100 micromol/L and 200 micromol/L groups 4429.43 +/- 391.41 and 6187.5 +/- 307.53), compared with untreated luciferase reporter gene group (3361.81 +/- 316.85) (P < 0.05 and P < 0.01, respectively), and induced MAPK phosphorylation expression (P < 0.05 and P < 0.01, respectively). Then these effects of bezafibrate upregulated eNOS expressions could be blocked by PPARalpha antagonist, MAPK and PI3K inhibitor while not affected by PKC and MEK inhibitor (P < 0.01).</p><p><b>CONCLUSIONS</b>Bezafibrate can upregulate eNOS expression, enhance eNOS-ser-1179 site phosphorylation, increase NO production and stabilize eNOS mRNA through PPARalpha dependent pathway and PPARalpha independent MAPK and PI3K pathways. This mechanism provides additional anti-atherosclerotic and anti-hypertension benefits of bezafibrate in addition of lipid-lowering effects.</p>


Subject(s)
Animals , Bezafibrate , Pharmacology , Cattle , Cells, Cultured , Endothelial Cells , Metabolism , Endothelium, Vascular , Gene Expression Regulation , MAP Kinase Signaling System , Nitric Oxide Synthase Type III , Genetics , Metabolism , PPAR alpha , Metabolism , Phosphatidylinositol 3-Kinases , Metabolism
18.
Chinese Journal of Cardiology ; (12): 1122-1131, 2005.
Article in Chinese | WPRIM | ID: wpr-252999

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the angiogenetic effects of endogenous and exogenous epoxyeicosatrienoic acids (EET) and the relevant signaling mechanisms involved.</p><p><b>METHODS</b>Bovine aortic endothelial cells (BAEC) were incubated with synthetic EETs or infected with recombinant adeno-associated viruses (rAAV) containing CYP2C11-CYPOR, CYP2J2 or CYP102 F87V mutant to increase endogenous expression levels of EETs. BAEC proliferation measured by cell counting and chromatometry, migration assessed by transwell analysis, and capillary formation determined by chicken embryo chorioallantoic membrane assays (CAM) and tube formation tests on matrigel and angiogenesis were analysed in vivo. The potential involvement of various signaling pathways were explored using selective inhibitors.</p><p><b>RESULTS</b>Transfection with rAAV-2C11OR, rAAV-2J2 or rAAV-F87V promoted BAEC proliferation, migration, and capillary tubule formation. However, the effects of EETs on proliferation, migration and capillary tubule formation were attenuated by inhibitors of mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3 kinase)/Akt pathways, and partially attenuated by endothelial nitric oxide synthase (eNOS) inhibitor, but not by a protein kinase C inhibitor. In a rat ischemic hind limb model, rAAV-mediated epoxygenase transfection induced angiogenesis.</p><p><b>CONCLUSIONS</b>Arachidonic acid epoxygenase and its metabolites can promote angiogenesis through activating MAPK and PI3 kinase/Akt signaling pathways, and to some extent, the eNOS pathway, and the angiogenic effects may provide protection to ischemic tissues.</p>


Subject(s)
8,11,14-Eicosatrienoic Acid , Metabolism , Pharmacology , Animals , Cattle , Cell Proliferation , Cells, Cultured , Chick Embryo , Cytochrome P-450 Enzyme System , Metabolism , Endothelial Cells , Metabolism , Humans , Male , Mitogen-Activated Protein Kinases , Metabolism , Neovascularization, Physiologic , Phosphatidylinositol 3-Kinases , Metabolism , Rats , Rats, Wistar , Signal Transduction , Transfection
19.
Chinese Journal of Cardiology ; (12): 534-535, 2005.
Article in Chinese | WPRIM | ID: wpr-334665

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between microalbuminuria (MA) and plasma high sensitivity C reactive protein (hsCRP).</p><p><b>METHOD</b>The plasma hsCRP level and urine albumin level of 746 Chinese people were detected with ELISA and analyzed, respectively.</p><p><b>RESULT</b>The hsCRP level of MA group increased significantly compared with the control group (2.23 +/- 3.35 mg/L vs 1.68 +/- 2.58 mg/L, P < 0.05), the incidence of MA increased significantly when the plasma hsCRP level was beyond 0.77 mg/L (P < 0.05); logistic analysis showed that the occurrence of MA was associated with the plasma hsCRP level (OR = 1.153, P < 0.05).</p><p><b>CONCLUSION</b>Increased plasma hsCRP was probably involved in the occurrence of MA, suggesting that inflammation may deteriorate endothelial dysfunction.</p>


Subject(s)
Aged , Albuminuria , Urine , Asian Continental Ancestry Group , C-Reactive Protein , Enzyme-Linked Immunosorbent Assay , Female , Humans , Inflammation , Male , Middle Aged
20.
Chinese Journal of Epidemiology ; (12): 945-948, 2004.
Article in Chinese | WPRIM | ID: wpr-324985

ABSTRACT

<p><b>OBJECTIVE</b>To study the serum homocysteine (Hcy) distribution and characteristics in different sex and age groups in the community residents in Wuhan, and to analyse its associated factors with multi-stepwise regression analysis.</p><p><b>METHODS</b>The population under study was from three community areas in Wuhan. Demographic distribution and the correlation with other risk factors of serum Hcy were analyzed statistically.</p><p><b>RESULTS</b>(1) Geometric mean of serum Hcy was 14.43 micromol/L in males and 10.89 micromol/L in females with P <0.001. (2) Hcy of per age level in males was also higher (P <0.001). (3) The prevalence rate of hyperhomocysteinemia was 23.94% in the general population in Wuhan. The prevalence rate of hyperhomocysteinemia in males was 2.62 times higher than in females. (4) Multi-stepwise regression analysis showed that Hcy had different affecting factors in males and females. The affecting factors of Hcy in males were daily cigarettes smoking, urine micro-albumin (UMALB) and times of exercise per week. The affecting factors of Hcy in females were duration of exercise each time, weight, triglyceride (TG), high-density lipoprotein (HDL), urine micro-albumin (UMALB) and age.</p><p><b>CONCLUSIONS</b>(1) Hcy at the population level was significantly different by sex and age. (2) Population living in the community in Wuhan had a higher serum level and prevalence rate of Hcy comparing to some other cities in China and even in developed countries. (3) The important affecting factors of Hcy in population also showed sex difference, unlike the reports from other countries or other areas in China. Serum Hcy seemed to be affected by environmental and other factors.</p>


Subject(s)
Adolescent , Adult , Age Factors , Aged , China , Female , Homocysteine , Blood , Humans , Male , Middle Aged , Population Groups , Reference Values , Regression Analysis , Sex Factors
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