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1.
Chinese Journal of Biotechnology ; (12): 2991-3004, 2021.
Article in Chinese | WPRIM | ID: wpr-921401

ABSTRACT

Flowering is a critical transitional stage during plant growth and development, and is closely related to seed production and crop yield. The flowering transition is regulated by complex genetic networks, whereas many flowering-related genes generate multiple transcripts through alternative splicing to regulate flowering time. This paper summarizes the molecular mechanisms of alternative splicing in regulating plant flowering from several perspectives, future research directions are also envisioned.


Subject(s)
Alternative Splicing/genetics , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Flowers/genetics
2.
Chinese Journal of Biotechnology ; (12): 2645-2657, 2021.
Article in Chinese | WPRIM | ID: wpr-887830

ABSTRACT

Lysine acetylation is one of the major post-translational modifications and plays critical roles in regulating gene expression and protein function. Histone deacetylases (HDACs) are responsible for the removal of acetyl groups from the lysines of both histone and non-histone proteins. The RPD3 family is the most widely studied HDACs. This article summarizes the regulatory mechanisms of Arabidopsis RPD3 family in several growth and development processes, which provide a reference for studying the mechanisms of RPD3 family members in regulating plant development. Moreover, this review may provide ideas and clues for exploring the functions of other members of HDACs family.


Subject(s)
Arabidopsis/metabolism , Histone Deacetylases/metabolism , Histones , Plant Development/genetics
3.
Chinese Journal of Biotechnology ; (12): 253-265, 2021.
Article in Chinese | WPRIM | ID: wpr-878559

ABSTRACT

Based on observing the cytological characteristics of the flower buds of the functional male sterile line (S13) and the fertile line (F142) in eggplant, it was found that the disintegration period of the annular cell clusters in S13 anther was 2 days later than that of F142, and the cells of stomiun tissue and tapetum in F142 disintegrated on the blooming day, while it did not happen in S13. The comparative transcriptomic analysis showed that there were 1 436 differential expression genes (DEGs) (651 up-regulated and 785 down-regulated) in anthers of F142 and S13 at 8, 5 days before flowering and flowering day. The significance analysis of GO enrichment indicated that there were more unigene clusters involved in single cell biological process, metabolism process and cell process, and more catalytic activity and binding function were involved in molecular functions. Through KEGG annotation we found that the common DEGs were mainly enriched in the biosynthesis of secondary metabolites, metabolic pathway, protein processing in endoplasmic reticulum, biosynthesis of amino acids, carbon metabolism and plant hormone signal transduction. The fifteen genes co-expression modules were identified from 16 465 selected genes by weighted gene co-expression network analysis (WGCNA), three of which (Plum2, Royalblue and Bisque4 modules) were highly related to S13 during flower development. KEGG enrichment showed that the specific modules could be enriched in phenylpropanoid biosynthesis, photosynthesis, porphyrin and chlorophyll metabolism, α-linolenic acid metabolism, polysaccharide biosynthesis and metabolism, fatty acid degradation and the mutual transformation of pentose and glucuronic acid. These genes might play important roles during flower development of S13. It provided a reference for further study on the mechanism of anther dehiscence in eggplant.


Subject(s)
Flowers/genetics , Gene Expression Profiling , Gene Expression Regulation, Plant , Humans , Infertility, Male , Male , Metabolic Networks and Pathways/genetics , Solanum melongena/genetics , Transcriptome/genetics
4.
Chinese Journal of Biotechnology ; (12): 142-148, 2021.
Article in Chinese | WPRIM | ID: wpr-878549

ABSTRACT

WRKY transcription factors are one of the largest families of transcription factors in higher plants and involved in regulating multiple and complex growth and development processes in plants. WRKY12 is a typical member of WRKY family. This article summarizes recent research progresses on the regulatory mechanism of WRKY12 in multiple growth and development processes, and analyzes the functional differences between WRKY12 and WRKY13. It provides a useful reference for further studying the molecular mechanism of WRKY12 in plant complex developments. It also provides clearer research ideas and reference strategies for exploring the self-regulation of other WRKY member and the mutual regulatory relationships between different WRKY family genes.


Subject(s)
Gene Expression Regulation, Plant , Humans , Phylogeny , Plant Development/genetics , Plant Proteins/metabolism , Plants/metabolism , Stress, Physiological , Transcription Factors/metabolism
5.
Chinese Journal of Biotechnology ; (12): 2277-2286, 2020.
Article in Chinese | WPRIM | ID: wpr-878485

ABSTRACT

MYB transcription factor is one of the largest transcription families and involved in plant growth and development, stress response, product metabolism and other processes. It regulates the development of plant flowers, especially anther development, a key role in the reproduction of plant progeny. Here, we discuss the regulatory effects of MYB transcription factors on the development of anther, including tapetum development, anther dehiscence, pollen development, carbohydrates and hormone pathways. We provide a reference for the further study of the regulation mechanism and network of plant anther development.


Subject(s)
Arabidopsis/metabolism , Flowers/genetics , Gene Expression Regulation, Plant , Humans , Pollen/genetics , Reproduction , Transcription Factors/metabolism
6.
Chinese Journal of Biotechnology ; (12): 2051-2065, 2020.
Article in Chinese | WPRIM | ID: wpr-878465

ABSTRACT

Plant trichomes are special structures that originate from epidermal outgrowths. Trichomes play an important role in plant defense against pests and diseases, and possess economic and medicinal values. Study on molecular mechanism of plant trichomes will contribute to the molecular design breeding and genetic improvement of crops. In recent years, the regulation mechanism of trichome development has been basically clarified in the model plant Arabidopsis thaliana, while great progresses are also found in other plant species. In this review, we focus on the developmental regulation of trichome formation from gene and phytohormones levels in Arabidopsis and cotton (with unicellular trichomes), as well as in tomato and Artemisia annua (with multicellular trichomes). The research progress associated with trichomes is also introduced in other typical monocotyledons and dicotyledons. Finally, the research and application of plant trichomes are prospected.


Subject(s)
Arabidopsis/genetics , Gene Expression Regulation, Plant , Gossypium/genetics , Lycopersicon esculentum , Plant Growth Regulators/metabolism , Trichomes/genetics
7.
Chinese Journal of Biotechnology ; (12): 838-848, 2020.
Article in Chinese | WPRIM | ID: wpr-826892

ABSTRACT

DNA methylation is an epigenetic modification that forms an important regulation mechanism of gene expression in organisms across kingdoms. Aberrant patterns of DNA methylation can lead to plant developmental abnormalities. In this article, we briefly discuss DNA methylation in plants and summarize its functions and biological roles in regulating gene expression and maintaining genomic stability, plant development, as well as plant responses to biotic and abiotic stresses. We intended to provide a concise reference for further understanding of the mechanism of DNA methylation and potential applications of epigenetic manipulation for crop improvement.


Subject(s)
Crop Production , DNA Methylation , Epigenesis, Genetic , Gene Expression Regulation, Plant , Genomic Instability , Plants , Genetics , Research , Stress, Physiological
8.
Chinese Journal of Biotechnology ; (12): 1170-1180, 2020.
Article in Chinese | WPRIM | ID: wpr-826861

ABSTRACT

HDA9, a member of the deacetylase family, plays a vital role in regulating plant flowering time through flowering integrator SOC1 and AGL24. However, it remains elusive how HDA9 interacts with SOC1 and AGL24 in flowering time control. Here, HDA9 was cloned in Brassica juncea and then its three active sites were separately replaced with Ala via overlap extension PCR. Thus, mutants of HDA9(D172A), HDA9(H174A) and HDA9(D261A) were constructed and fused into the pGADT7 vector. The yeast one-hybrid assays indicated that HDA9 mutants remained the interactions with the promoters of SOC1 and AGL24. Furthermore, the aforementioned results were confirmed in the dual luciferase assays. Interestingly, the DNA-protein interactions were weakened significantly due to the mutation in the three active sites of HDA9. It suggested that flowering signal integrator SOC1 and AGL24 were regulated by the key amino acid residues of 172th, 174th and 261th in HDA9. Our results provide valuable information for the in-depth study of the biological function and molecular regulation of HDA9 in Brassica juncea flowering time control.


Subject(s)
Flowers , Genetics , Gene Expression Regulation, Plant , Genetics , Mustard Plant , Genetics , Mutation , Plant Proteins , Genetics , Metabolism , Promoter Regions, Genetic , Genetics
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