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1.
Medicina (B.Aires) ; 68(6): 423-427, nov.-dic. 2008. tab
Article in English | LILACS | ID: lil-633581

ABSTRACT

The purpose of this study was to characterize and quantify cells involved in immune response in metastasis-free regional lymph nodes (RLNs) draining different human epithelial tumors and compare them (by immunohistochemistry) with control lymph nodes from patients with non malignant diseases. We showed that T cells number was decreased in RLNs as compared to the controls with reduction in both CD4+ T cells and CD8+ T cells subsets and an inverted ratio (CD4+: CD8+). B lymphocytes and follicular dendritic cells were decreased with respect to the controls. S100+ dendritic cells (DCs) and mature DCs were detected in T dependent areas. Their mean number was significantly lower as compared to control. Immature DCs were significantly diminished compared to RLN and control nodes. CD57+ cells, follicular T helper cells and/or NK cells, were localized in the clear zone of germinal centres and their mean number was significantly increased. There were no CD57+ cells in hypoplastic follicles. In this study we show that RLNs draining human cancer present reduction in almost all immune cells, except CD57+ cells. These findings may be related to the deficient anti-tumor immune response in patients with cancer and subsequent tumor progression.


El objetivo del trabajo fue caracterizar y cuantificar utilizando inmuno-histoquímica, las células involucradas en la respuesta inmune en ganglios linfáticos regionales (GLRs) que drenan distintos tumores epiteliales malignos humanos y compararlas con ganglios controles (GLCs) provenientes de pacientes sin enfermedad neoplásica maligna. Determinamos que los GLRs presentaban una marcada depleción de linfocitos B y T, células dendríticas (CD) foliculares y CD interdigitantes maduras respecto a los controles. En los linfocitos T, además de estar disminuidos, se observó una inversión de la relación T CD4+: T CD8+, a favor de los T CD8+. La depleción de CD inmaduras fue mayor respecto a las maduras. Las células CD57+, células foliculares T helper y/o células NK, localizadas en las zonas claras de los centros germinativos, presentaron un marcado incremento en los GLRs comparados con los GLCs, excepto en los casos de ganglios linfáticos con folículos hipoplásicos. En este estudio, demostramos que los GLRs que drenan carcinomas humanos presentan una significativa reducción en casi todas las células de la respuesta inmune, excepto las células NK. Estos hallazgos podrían estar relacionados con la deficiente respuesta antitumoral de los pacientes con cáncer y la subsiguiente progresión tumoral.


Subject(s)
Adult , Aged , Humans , Middle Aged , Dendritic Cells/cytology , Dendritic Cells/immunology , Lymph Nodes/immunology , Lymphocyte Subsets/immunology , Neoplasms/immunology , T-Lymphocytes/cytology , B-Lymphocytes/immunology , Case-Control Studies , Immunity, Cellular , Lymph Nodes/pathology , Lymphatic Metastasis/immunology , Lymphatic Metastasis/pathology , Neoplasms/pathology , T-Lymphocytes/immunology
3.
Medicina (B.Aires) ; 62(1): 73-82, 2002.
Article in Spanish | LILACS | ID: lil-305545

ABSTRACT

Breast cancer is the most frequently diagnosed cancer among women in the western world. In spite of a great deal of scientific information in relation to its origin, of new diagnostic methods and treatments, mortality rate due to breast cancer has virtually remained stable over the past 20 years. Recent advances in molecular biology have improved the understanding of the basic biology of breast cancer. This fact has led to the identification of new tumor markers the ultimate goal of which is to reduce mortality by identifying women at risk as well as predicting the prognosis of existing disease and the response to different therapies. This article focuses on both traditional and new molecular markers, their clinical utility and their relevance in the routine evaluation of patients with breast cancer.


Subject(s)
Humans , Female , Biomarkers, Tumor , Breast Neoplasms , Biomarkers, Tumor , Genetic Markers
4.
Quirón ; 30(3): 16-21, ago. 1999. ilus
Article in Spanish | LILACS | ID: lil-261797

ABSTRACT

Apoptosis is an active form of cell death that can be observed both in physiological and pathological conditions. The apoptotic process (programmed cell death) can be studied "in vivo" and "in vitro" conditions. It is a programmed and organized energy dependent response to cellular media changes, and sometimes it requires genetic expression The typical morphological changes of apoptosis are: condensation of the cytoplasm and the nuclear chromatin, early cytoplasmatic blebs, DNA degradation preferentially between the nucleosomes and generation of apoptotic bodies. The aim of this paper is to describe an experimental model of apoprosis using Hep-2 cul-tured cells, and as inductor agent a culture medium solution with Paclitaxel at the concentra-tion of 5 (M/ml during 20 hours. Paclitaxel is a potent antitumor drug which acts by stabilizing microtubules (irreversible tubulin polymerization), preventing normal mitosis, and resulting in a blockage of the cell cycle at G2 - M and cell death from apoptosis. In the present work, we could appreciate the presence ofi apoptotic cells, cells blocked in mitosis, multinucleated interphase cells, and cells resembling controls. Our findings support the use of Paclitaxel for the development of experimental model of apoptosis "in vitro".


Subject(s)
Apoptosis , In Vitro Techniques , Cell Line , Paclitaxel
5.
Medicina (B.Aires) ; 59(5,pt.1): 477-86, 1999. tab, ilus
Article in Spanish | LILACS | ID: lil-247915

ABSTRACT

Las proteínal del shock térmico (Hsp) constituyen una família que se encuentra en forma constitutiva en todas las células pro y eucariotas. Cumplen diversas funciones fisiológicas: colaboran en la adquisición de la estructura terciarua de las proteínas en formación, interviniendo en su ensamble, translocación y secreción así como también en la degradación o reparación de proteínas anormales, actuando como chaperonas moleculares. Cuando las células son sometidas a distintos estímulos como el estrés del shock calórico, radiaciones, diversas drogas, infecciones virales, etc, las Hsp se sobreexpresan. De esta manera confieren protección a las células, volviéndolas resistentes a la apoptosis. Esta familia de proteínas comprende numerosos miembros que se agrupan según su peso molecular. En los seres humanos, las Hsp se expresan también en tejidos neoplásicos de ovario, endometrio, mama, aparato digestivo, etc. En algunos casos, la sobreexpresión está asociada a mal pronóstico de la enfermedad debido a que podría favorecer el proceso metastásico. Algunos autores las correlacionan tanto con la proliferación como con la diferenciación de los tejidos neoplásicos. Recientes estudios muestran su influencia en el desarrollo de la resistencia a drogas quimioterapéuticas. En enfermedades autoimunes, como artritis reumatoidea, las Hsp pueden suprimir la respuesta inflamatoria. En otras enfermedades pueden resultar inmunógenas por sí mismas. Por consiguiente su papel en el sistema inmune aún no está bien definido.


Subject(s)
Heat-Shock Proteins/physiology
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