ABSTRACT
OBJECTIVE To study the protective mechanism of Bawei chenxiang powder containing serum on H9c2 cells injured by oxygen-glucose deprivation (OGD). METHODS H9c2 cells were divided into blank group, model group and Bawei chenxiang powder low-dose, medium-dose and high-dose groups (the dose of drug containing serum 2.5, 8, 12 g/kg). H9c2 cells were cultured in vitro to establish OGD model. After intervention with drug-containing serum, survival rate of cell was detected. The cell morphology was observed; the levels of lactate dehydrogenase (LDH), creatine kinase (CK), superoxide dismutase (SOD), catalase (CAT), respiratory chain complexⅠ (ComplexⅠ), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were detected. The contents of reactive oxygen species (ROS), mitochondrial membrane potential and apoptosis were also detected. The expressions of oxidative stress-related proteins [Kelch ECH association protein 1 (Keap1), nuclear factor erythroid 2- related factor 2 (Nrf2), heme oxygenase 1 (HO-1), NADH oxidoreductase coenzyme 10 (Ndufa10), thioredoxin (Trx)] and apoptosis-related proteins [B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax), Caspase-3 and cytochrome C (Cytc)] were detected. RESULTS Compared with blank group, the cell morphology of model group was damaged; the levels of LDH, CK and MDA were significantly increased (P<0.01), while the levels of CAT, ComplexⅠ, SOD and GSH-Px and mitochondrial membrane potential were significantly decreased (P<0.01). The content of intracellular ROS and apoptotic rate were significantly increased (P<0.01); the expressions of oxidative stress-related proteins (Keap1, Nrf2, HO-1, Ndufa10 and Trx) and pro- apoptosis proteins (Bax, Caspase-3 and Cytc) were significantly increased (P<0.05), while the expression of anti-apoptotic protein Bcl-2 was significantly decreased (P<0.05). After administration of Bawei chenxiang powder containing serum, the cell morphology improved, and most of the above indexes were significantly reversed (P<0.05 or P<0.01).CONCLUSIONS Bawei chenxiang powder containing serum E-mail:345783110@qq.com has a good protective effect on H9c2 cells damaged by OGD,the mechanism of which is related to the reduction of oxidative damage and inhibition of cell apoptosis.