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1.
Chinese Journal of Laboratory Medicine ; (12): 253-259, 2017.
Article in Chinese | WPRIM | ID: wpr-608613

ABSTRACT

Objective To verify and valuate the performance of small dense low-density lipoprotein cholesterol (sdLDL-C) detection by the direct clearance method and evaluate its preliminary clinical application in acute coronary syndrome (ACS).Methods Case control study:The performance (accuracy,precision,linearity) of sdLDL-C was assessed by direct clearance method.In 143 cases of ACS patients selected from Cardiology Department and Emergency Department of Shangdong Provincial Hospital from April to October in 2016,with 100 cases male,female 43 cases,including acute myocardial infarction (AMI)group of 59 cases,unstable angina pectoris (UAP) group of 84 cases;83 cases of healthy volunteers as a control group selected from health physical examination center of Shandong Provincial Hospital,with 59cases male,female 24 cases.Levels of sdLDL-C,total cholesterol (TCH),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C),apolipoprotein A (ApoA I),apolipoprotein B (ApoB),lipoprotein (a) (Lpa) and high sensitive C-reactive protein (Hs-CRP) were detected separately by automatic biochemical analyzer.Non high density lipoprotein cholesterol (non-HDL-C) equals TCH minus HDL-C.x2 test,t test,one-way ANOVA,Pearson correlation and multiple linear regression analysis were used as statistical methods.Results The within-lot or between-lot variation was 2.85% and 3.36%.Methodological comparison:regression equation Y =0.984X + 0.018,r2 =0.966,t =-0.191,P =0.850.There was a good linear correlation (Y =1.026X + 0.007,r2 =0.999) between theoretical values and actual detection results in range of 0.15-2.65 mmol/L.SdLDL-C concentrations were positive correlated with TCH,non-HDL,LDL-C,TG,ApoB (r =0.758,0.848,0.839,0.514,0.885,respectively,P <0.01),and negative correlated with HDL-C (r =-0.224,P =0.001),but no correlation with APOA I,Lpa and Hs-CRP(r =-0.021,0.050,0.003,respectively,P > 0.05).Multiple linear regression analysis showed that the factors influencing sdLDL-C level were HDL-C,ApoB,LDL-C and TG.The levels of sdLDL-C,TG in the ACS group were significantly higher than those in the control group (t =3.415,4.660,respectively,P < 0.01),but no difference between the two groups in the levels of TCH,non-HDL-C and LDL-C (t=-1.831,-0.452,-1.398,respectively,P >0.05).Comparing AMI group with control group,sdLDL-C,TG and Hs-CRP were significantly higher than the control group (P =0.000,0.000,0.000,respectively),but TCH,LDL-C and non-HDL were similar between the two groups (P =0.800,0.320,0.120,respectively);Comparing UAP group with control group,TG and Hs-CRP were higher than control group (P =0.001,0.047,respectively),TCH and LDL-C were significantly lower than the control group (P =0.003,0.008,respectively),but sdLDL-C had no difference (P =0.305);Comparing AMI group with UAP group,sdLDL-C,TCH,LDL-C and Hs-CRP were significantly higher than UAP group (P =0.000,0.003,0.001,0.000,respectively),and TG were no statistical significance (P =0.473).Conclusions Direct clearance method can meet the requirement of sdLDL-C detection.sdLDL-C level can assess the metabolism of blood lipids and be used as an independent risk factor and predictive index of ACS,superior to LDL-C.

2.
Chinese Journal of Current Advances in General Surgery ; (4): 841-844, 2017.
Article in Chinese | WPRIM | ID: wpr-703767

ABSTRACT

Objective:To evaluate the activity of superoxide dismutase (SOD) in patients with hepatitis B and Hepatocellular carcinoma.Methods:63 cases of patients with hepatitis B,101 cases of patients with Hepatocellular carcinoma (HCC) and 58 cases of normal control were collected,serum enzyme activity of SOD was detected by enzyme method.Variance analysis were applied to analyze the difference of SOD activity in different groups.Receiver operating characteristic (ROC) were applied to analyze the diagnosis performance of SOD in hepatitis B and HCC patients.Results:SOD activity from high to low in turn was hepatitis B group191.500 U/mL,healthy contol group(179.766 ± 13.546 U/mL) and HCC group 150.000 U/mL,there were significant difference when compareing any two grougs (P<0.05).SOD=187.20 U/mL could be used as diagnosis threshold in hepatitis B patients,the sensitivity was 65.08% and the specificity was 75.86%.SOD=166.00 U/mL can be used as diagnosis threshold in HCC patients,the sensitivity was 82.18% and the specificity was 84.48%.SOD=170.40 U/mL could be used as diagnosis threshold in HCC patients with AFP<20 ng/mL,the sensitivity was 82.76% and the specificity was 79.31%.Conclusions SOD activity varies in healthy person,hepatitis B and HCC patients.The changes of SOD activity could help us to understand whether there was transformation from hepatitis B to HCC.

3.
Chinese Journal of Current Advances in General Surgery ; (4): 680-683, 2017.
Article in Chinese | WPRIM | ID: wpr-665796

ABSTRACT

Objective:To study the activity of superoxide dismutase (dismutase superoxide,SOD) in peripheral blood of patients with thyroid associated diseases.Methods:84 cases of patients with thyroid cancer,21 cases of patients with nodular goiter,75 cases of patients with hyperthyroidism,56 cases of patients with hypothyroidism and 63 cases of normal control were collected,serum enzyme activity of SOD was detected by enzyme method,FT3,FT4 and TSH levels were detected by electrochemiluminescence assay.Student-t,Mann-whitney-U or Kruskal-Wallis was applied to analyse the different of SOD activity in different groups.Pearson correlation analysis were applied to analyze the correlation of SOD activity with thyroid hormone levels.Results:SOD activity in thyroid cancer group (164.536 ± 18.095) U/ml was significantly lower than that in hyperthyroidism group (173.376 ± 15.942) U/ml,hypothyroidism group (174.827 ± 19.895) U/ml and normal control group(179.529 ±11.625) U/ml(P<0.05),SOD activity in nodular goiter group(157.667 ± 15.189) U/ml was significantly lower than that in hyperthyroidism group (173.376 ± 15.942) U/ml,hypothyroidism group (174.827 ± 19.895) U/ml and normal controls (179.529 ± 11.625) U/ml (P<0.05).There were positive correlation of FT3,FT4 levels respectively with SOD activity in thyroid cancer group(r=0.346 P<0.05,r=0.3278,P<0.05),and there was positive correlation of FT3 level with SOD activity in normal control group (r=-0.5522.P<0.05).Conclusions:SOD activity decreased in nodular goiter and thyroid cancer,so it could be used to observe the damage of thyroid tissue.Furtherly,it might be used as an auxiliary index in the diagnosis of thyroid tumors.

4.
Chinese Journal of Primary Medicine and Pharmacy ; (12): 961-962,963, 2014.
Article in Chinese | WPRIM | ID: wpr-598996

ABSTRACT

Objective To explore the characterization of antibiotic resistance in hospitalized elderly patients with lower respiratory tract infection caused by Pseudomonas Aeruginosa ,and provide scientific basis for clinical ra-tional use of antimicrobial drugs and prevent the emergence of pan-drug resistant bacteria .Methods The drug sensi-tive tests of 347 hospitalized elderly patients with lower respiratory tract infection caused by Pseudomonas Aeruginosa in Shandong provincial hospital affinited to Shandong university from May 2010 to June 2012 were analyzed retrospec-tively.Results The antimicrobial resistance of the 347 cases was rather serious .The resistant rates of ampicillin and cefazolin were both 100.0%.Followed by ampicillin/sulbactam,tobramycin,sulfamethoxazole/trimethoprim,the re-sistant rates were 95.1%,86.8%and 83.6%respectively.The resistant rate of imipenem has reached 19.6%,and the detection rate of pan-drug bacteria was 4.9%.Conclusion The antibiotic resistance in hospitalized elderly pa-tients with lower respiratory tract infection caused by Pseudomonas Aeruginosa has become more and more serious .In order to prevent the emergence and prevalence of pan-drug resistant bacteria , antibacterial agents should be chosen reasonably according to the results of drug sensitive tests .

5.
Chinese Journal of Microbiology and Immunology ; (12): 309-314, 2012.
Article in Chinese | WPRIM | ID: wpr-428842

ABSTRACT

Objective To construct series of reporter plasmids with truncated and deleted hTERT promoter.Methods Gene fragments of hTERT promoter was amplified by PCR and cloned into pGL3-Basic to construct luciferase reporter vectors.Dual luciferase assays were performed with cell lysates of HepG2 and COS-7 cells cotransfected with hTERT promoter reporter plasmids and pRL-TK.Results Series of luciferase reporter plasmids with truncated and deleted hTERT promoter were successfully constructed and respectively named pGL3B-895,pGL3B-371,pGL3B-DELS2,pGL3B-349,pGL3B-329,pGL3B-318,pGL3B-306.Dual luciferase reporter assays showed that all the reporter vectors have promoter activity both in HepG2 and COS-7.Conclusion Series of luciferase reporter plasmids with truncated and deleted hTERT promoter were successfully constructed,and their promoter activity were verified.These plasmids provide necessary experimental naterials for further investigation of regulation of hTERT during hepatocarcinoma development.

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