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Article in Chinese | WPRIM | ID: wpr-790789


Objective To develop a method for the determination of total anthraquinones in Xiaofan lotion by HPLC.Methods Rhubarb anthraquinones were simultaneously separated and assayed on a Kromasil C18 column(250 mm×4.6 mm,5 μm) with the mobile phase of 0.1%phosphoric acid-methanol (15∶85) at 30 ℃.The detection wavelength was 207 nm.Results The linear relationship is good for Aloe-emodin and other five standard ingredients.The average recovery was 99.45%, RSD 1.68%.Conclusion The method is simple, rapid and accurate.It is suitable for determination of total anthraquinones in Xiaofan lotion.

Article in Chinese | WPRIM | ID: wpr-251188


<p><b>OBJECTIVE</b>To establish the unitive multicomponent quality system bridged macrostate mathematic model parameters of material quality and microstate component concentration for Chinese materia medica (CMM).</p><p><b>METHOD</b>According to law of biologic laws of thermodynamics, the state functions of macrostate qulity of the CMM were established. The validation test was carried out as modeling drug as alcohol extract of Radix Rhozome (AERR), their enthalpy of combustion was determined, and entropy and the capability of information by chromatographic fingerprint were assayed, and then the biologic apparent macrostate parameters were calculated.</p><p><b>RESULT</b>The biologic macrostate mathematic models, for the CMM quality controll, were established as parameters as the apparent equilibrium constant, biologic enthalpy, Gibbs free energy and biologic entropy etc. The total molarity for the 10 batchs of AERR were 0.153 4 mmol x g(-1) with 28.26% of RSD, with the average of apparent equilibrium constants, biologic enthalpy, Gibbs free energy and biologic entropy were 0.039 65, 8 005 J x mol(-1), -2.408 x 10(7) J x mol(-1) and - 8.078 x 10(4) J x K(-1) with RSD as 6.020%, 1.860%, 42.32% and 42.31%, respectively.</p><p><b>CONCLUSION</b>The macrostate quality models for CMM can represent their intrinsic quality for multicomponent dynamic system such as the CMM, to manifest out as if the forest away from or tree near from to see it.</p>

Drugs, Chinese Herbal , Materia Medica , Models, Theoretical , Quality Control , Thermodynamics
Article in Chinese | WPRIM | ID: wpr-281675


<p><b>OBJECTIVE</b>To establish the theories and methods to determine apparent solubility paraneters of multiple components for the Chinese materia medica (CMM) with HPLC fingerprint.</p><p><b>METHOD</b>The mathematical functional expresses to determine the apparent solubility parameters for multiple constituents were established according to total quantum geometrical average retention time (TQGART) for HPLC fingerprint that characterized the entirety tendencies for all-over chromatographic peaks, validated by the aloe-emodin's solubility parameters which had been determined. The HPLC for the aloe-emodin's solubility parameters were carried out with an Alltech Apollo C18 as column, Acetone: Water as mobile phase, gradient elution,flow rate as 1.0 mL x min(-1), the detection wave-length as 430 nm and the temperature as 30 degrees C.</p><p><b>RESULT</b>The mathematical functional model between the TQGART of HPLC chromatographic fingerprint and the total quantum apparent solubility parameters was established and used to determine the aloe-emodin's mixture solubility parameter as 36.12 J(1/2) x cm(-3/2), nearly equal to 35.57, 36.07 J(1/2) x cm(-3/2) calculated by ration of peak area ratios and molecular fraction, respectively.</p><p><b>CONCLUSION</b>The TQGART of HPLC fingerprint can be used to determine simultaneously the apparent or single intrinsic solubility parameters for total quantum or intrinsic solubility parameters for single in multiple constitute systems, by which theoretical and technologic platform to study the compatibility rule and dosage form reform of the single CMM will be established.</p>

Anthraquinones , Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Models, Theoretical , Solubility